嗜铬细胞瘤分子遗传学研究进展

嗜铬细胞瘤分散发型和家族型两大类。近年来 ,家族型嗜铬细胞瘤的分子遗传学研究取得了重要进展 ,RET原癌基因突变和 VHL 基因的失活可能分别是 MEN2和 VHL 病的致病因素。散发型嗜铬细胞瘤发病的分子机制尚未阐明 ,可能涉及癌基因的激活 ,抑癌基因的缺失以及凋亡障碍和端粒酶活性增强等诸多因素     

嗜铬细胞瘤的分子遗传学研究

Pheochromocytomas and paragangliomas are tumors of the autonomic nervous system. It haspreviously been estimated that approximately 10% ～ 15% of pheochromocytomas are caused by hereditary, butrecent studies indicate that the frequencies of germline mutations in RET, VHL, SDHB, SDHD together approa-ches 30%. According to this situation, genetic testing is suggested. In this article the known genetic syndromesare commonly associated with pheochromocytoma, its genes'structure, germline mutations, the association be-tween the genotype and the phenotype, and the possible pathogenesis are reviewed.     

嗜铬细胞瘤的分子遗传学研究

Pheochromocytomas and paragangliomas are tumors of the autonomic nervous system. It haspreviously been estimated that approximately 10% ～ 15% of pheochromocytomas are caused by hereditary, butrecent studies indicate that the frequencies of germline mutations in RET, VHL, SDHB, SDHD together approa-ches 30%. According to this situation, genetic testing is suggested. In this article the known genetic syndromesare commonly associated with pheochromocytoma, its genes'structure, germline mutations, the association be-tween the genotype and the phenotype, and the possible pathogenesis are reviewed.     

嗜铬细胞瘤的分子遗传学研究

Pheochromocytomas and paragangliomas are tumors of the autonomic nervous system. It haspreviously been estimated that approximately 10% ～ 15% of pheochromocytomas are caused by hereditary, butrecent studies indicate that the frequencies of germline mutations in RET, VHL, SDHB, SDHD together approa-ches 30%. According to this situation, genetic testing is suggested. In this article the known genetic syndromesare commonly associated with pheochromocytoma, its genes'structure, germline mutations, the association be-tween the genotype and the phenotype, and the possible pathogenesis are reviewed.     

嗜铬细胞瘤的分子遗传学研究

Pheochromocytomas and paragangliomas are tumors of the autonomic nervous system. It haspreviously been estimated that approximately 10% ～ 15% of pheochromocytomas are caused by hereditary, butrecent studies indicate that the frequencies of germline mutations in RET, VHL, SDHB, SDHD together approa-ches 30%. According to this situation, genetic testing is suggested. In this article the known genetic syndromesare commonly associated with pheochromocytoma, its genes'structure, germline mutations, the association be-tween the genotype and the phenotype, and the possible pathogenesis are reviewed.     

嗜铬细胞瘤的分子遗传学研究

Pheochromocytomas and paragangliomas are tumors of the autonomic nervous system. It haspreviously been estimated that approximately 10% ～ 15% of pheochromocytomas are caused by hereditary, butrecent studies indicate that the frequencies of germline mutations in RET, VHL, SDHB, SDHD together approa-ches 30%. According to this situation, genetic testing is suggested. In this article the known genetic syndromesare commonly associated with pheochromocytoma, its genes'structure, germline mutations, the association be-tween the genotype and the phenotype, and the possible pathogenesis are reviewed.     

嗜铬细胞瘤的分子遗传学研究

Pheochromocytomas and paragangliomas are tumors of the autonomic nervous system. It haspreviously been estimated that approximately 10% ～ 15% of pheochromocytomas are caused by hereditary, butrecent studies indicate that the frequencies of germline mutations in RET, VHL, SDHB, SDHD together approa-ches 30%. According to this situation, genetic testing is suggested. In this article the known genetic syndromesare commonly associated with pheochromocytoma, its genes'structure, germline mutations, the association be-tween the genotype and the phenotype, and the possible pathogenesis are reviewed.     

嗜铬细胞瘤的分子遗传学研究

Pheochromocytomas and paragangliomas are tumors of the autonomic nervous system. It haspreviously been estimated that approximately 10% ～ 15% of pheochromocytomas are caused by hereditary, butrecent studies indicate that the frequencies of germline mutations in RET, VHL, SDHB, SDHD together approa-ches 30%. According to this situation, genetic testing is suggested. In this article the known genetic syndromesare commonly associated with pheochromocytoma, its genes'structure, germline mutations, the association be-tween the genotype and the phenotype, and the possible pathogenesis are reviewed.     

嗜铬细胞瘤的分子遗传学研究

Pheochromocytomas and paragangliomas are tumors of the autonomic nervous system. It haspreviously been estimated that approximately 10% ～ 15% of pheochromocytomas are caused by hereditary, butrecent studies indicate that the frequencies of germline mutations in RET, VHL, SDHB, SDHD together approa-ches 30%. According to this situation, genetic testing is suggested. In this article the known genetic syndromesare commonly associated with pheochromocytoma, its genes'structure, germline mutations, the association be-tween the genotype and the phenotype, and the possible pathogenesis are reviewed.     

嗜铬细胞瘤的分子遗传学研究

Pheochromocytomas and paragangliomas are tumors of the autonomic nervous system. It haspreviously been estimated that approximately 10% ～ 15% of pheochromocytomas are caused by hereditary, butrecent studies indicate that the frequencies of germline mutations in RET, VHL, SDHB, SDHD together approa-ches 30%. According to this situation, genetic testing is suggested. In this article the known genetic syndromesare commonly associated with pheochromocytoma, its genes'structure, germline mutations, the association be-tween the genotype and the phenotype, and the possible pathogenesis are reviewed.     

嗜铬细胞瘤的分子遗传学研究

嗜铬细胞瘤和副神经节瘤为自主神经系统肿瘤。以前估计约10%-15%的嗜铬细胞瘤由遗传因素导致,但近年来的研究显示RET,VHL,SDHB, SDHC, SDHD等易感基因的胚系突变率已近30%。鉴于此,建议进行遗传检查。本文就各遗传综合症,相关基因结构,与嗜铬细胞瘤相关的基因变异,基因型与表型的关联以及可能的发病机制等研究进展等作一综述。     

嗜铬细胞瘤的分子遗传学研究

Pheochromocytomas and paragangliomas are tumors of the autonomic nervous system. It haspreviously been estimated that approximately 10% ～ 15% of pheochromocytomas are caused by hereditary, butrecent studies indicate that the frequencies of germline mutations in RET, VHL, SDHB, SDHD together approa-ches 30%. According to this situation, genetic testing is suggested. In this article the known genetic syndromesare commonly associated with pheochromocytoma, its genes'structure, germline mutations, the association be-tween the genotype and the phenotype, and the possible pathogenesis are reviewed.     

良恶性嗜铬细胞瘤鉴别诊断的分子生物化学研究进展

恶性嗜铬细胞瘤大约占嗜铬细胞瘤的10%,副神经节瘤中恶性比例更高些.由于从临床表现、组织病理上进行嗜铬细胞瘤的良恶性诊断均存在一定的局限性,近几年来不同国家的研究组运用分子生物学的手段对嗜铬细胞瘤的良恶性鉴别进行了研究,并取得了一定的进展:SDHB突变可能与恶性嗜铬细胞瘤关系较大,其他一些基因与分子标记物的联合应用也可能成为判断嗜铬细胞瘤潜在恶性的标志.本文就近几年在分子生物学方面对于良恶性嗜铬细胞瘤进行鉴别诊断所取得的进展作一综述.     

肺癌分子遗传学的新进展

本回顾子近年来与肺癌有关的癌基因,抑癌基因及其产物、凋亡相关基因在肺癌分型、发生、发展等方面的研究成果,以其对肺癌这个由多基因,多阶段,多步骤而导致的疾病的发生机制有进一步的认识。     

胃癌的遗传学研究进展

胃癌是人类常见的恶性肿瘤。本文就与胃癌相关的染色体异常、癌基因激活和抑癌基因失活、转移抑制基因异常表达、细胞周期调控紊乱、细胞粘附分子和信号传导异常及遗传不稳定性等作一综述，旨在深入探讨胃癌发生发展的遗传学机制。     

肿瘤分子遗传学研究方法进展

肿瘤的发生是具有其分子生物学基础的。自80年代以来许多技术相继开发应用到肿瘤的分子遗传学研究，并取得了显著的成效。这些检测DNA变异的手段按检测对象分为两类，一类主要用于基因组DNA的检测，如肿病原癌基因和抑癌基因的定位克降、比较基因组杂交、代表性差异分析；用于cDNA或mRNA水平的技术包括消减杂交、差异显示PCR、cDNA代表性差异分析对、DNA芯片及探针微列冲等。这些技术的应用为肿瘤的分子造传学研究开拓了一个崭新的领域。     

肿瘤分子遗传学研究方法进展

肿瘤发生是具有其分子生物学基础的，自８０年代以来许多技术相继开发应用到肿瘤的分子遗传学研究，并取得了显的成效，这些检测ＤＮＡ变异的手段按检测对象分析为两类：一类主要用于基因组ＤＮＡ的检测，如肿瘤原癌基因和抑癌基因的定位克隆，比较基因组杂交，代表性差异分析；用于ｃＤＮＡ或ｍＲＮＡ水平的技术包括消减杂交，差异显示ＰＣＲ，ｃＤＮＡ代表性差异分析，ＤＮＡ芯片及探针微列阵等，这些技术的应用为肿瘤的分子遗传     

膀胱嗜铬细胞瘤临床病理分析

1　材料与方法收集本院手术治疗的膀胱嗜铬细胞瘤 3例标本 ,均用10 %福尔马林液固定 ,常规制片 ,ＨＥ染色 ,光镜观察。石蜡切片做免疫组化 (ＡＢＣ法标记 ,ＤＡＢ显色 ) ,所用抗体Ｓ 10 0蛋白、ＮＳＥ、ＣｇＡ、ＣＫ均为Ｄａｋｏ公司产品。2　结果2 .1　临床资料　 3例均为女性 ,30～ 6 4岁。血尿 1例 ,高血压 2例 ,其中月经不调 1例。 1例自主无症状 ,在体检时偶然发现。肿瘤位于膀胱左、右侧壁黏膜下或肌层内。2 .2　病理形态2 .2 .1　眼观　肿瘤直径 1 5～ 2 5ｃｍ ,切面棕黄色 ,质细 ,部分有包膜 ,灶性区有陈旧性出血。2 .2 .2　镜检　…     

嗜铬细胞瘤切除术中的巡回配合

嗜铬细胞瘤是发生于嗜铬组织的肿瘤,约90％来源于肾上腺髓质。由于肿瘤或肾上腺髓质的嗜铬细胞分泌大量儿茶酚胺包括肾上腺素、去甲肾上腺素和多巴胺,表现为阵发性或持续性高血压以及代谢紊乱症候群等。手术切除肿瘤是唯一有效的治疗方法,手术中由于多种因素如气管插管和体位改变,挤压肿瘤,手术探查等均可刺激瘤体,使其立即释放大量的儿茶酚胺。在结扎肿瘤血管或切除肿瘤后,体内内源性儿茶酚胺突然减少,周围血管扩张致有效循环量不足可发生低血容量性休克,同时术中血压大幅度升降可导致心律失常甚至心力衰竭等。我院从1991年2月至1995年10月共行35例嗜铬细胞瘤切除术,均安全渡过手术关并痊愈出院,现将术中配合的方