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1.
Hu Y; Maxson W; Hoffman D; Ory S; Eager S; Dupre J; Worrilow K 《Human reproduction (Oxford, England)》1998,13(1):165-168
Commercially obtained Buffalo rat liver (BRL) cells were grown in monolayer
culture. The effect of BRL cell co-culture with assisted hatching on embryo
development, implantation and pregnancy was investigated in a population of
200 'first-time' in-vitro fertilization (IVF) patients, subdivided into
three groups according to the methods of fertilization [IVF;
intracytoplasmic sperm injection (ICSI); ICSI/IVF]. Assisted hatching was
performed on all embryos chosen for transfer. Following co-culture, the
overall embryo quality, implantation rate and pregnancy rates were not
significantly different from the controls. However, when grouped according
to fertilization method, co-culture was found to have an impact on
pregnancy and implantation rates in the group undergoing conventional IVF.
Using co- culture with assisted hatching, we were able to achieve a 58%
(38/65) clinical pregnancy rate with a 49% (32/65) live birth rate and a
26% (60/235) implantation rate. No changes in the pregnancy and
implantation rates were apparent in ICSI or ICSI/IVF subgroups. This is the
first prospective, randomly controlled study which reports the use of BRL
cell co-culture for human IVF for a large number of patients undergoing IVF
for the first time.
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2.
Pelinck MJ; De Vos M; Dekens M; Van der Elst J; De Sutter P; Dhont M 《Human reproduction (Oxford, England)》1998,13(4):960-963
The aim of the present study was to investigate pregnancy rates ensuing
from transfer of embryos with multinucleated blastomeres. In our in- vitro
fertilization (IVF)/intracytoplasmic sperm injection (ICSI) programme, 1735
embryo transfers were performed from January 1, 1995 to August 31, 1996. In
136 of these transfers at least one embryo with one or more multinucleated
blastomeres was present per transfer (study group). For each of these 136
transfers, two matched controls with transfer of exclusively mononucleated
embryos were selected (control group). Matching was carried out according
to age, method of fertilization (IVF or ICSI), number of transferred
embryos and quality score of transferred embryos. In the study group, there
were eight transfers of exclusively multinucleated embryos from which one
pregnancy ensued and 128 transfers in which multinucleated and
mononucleated embryos were transferred together leading to 23 pregnancies.
The overall clinical pregnancy rate per transfer was 16.9% in the study
group versus 28.7% in the control group (P = 0.01). The ongoing pregnancy
rate per transfer was 13.2% in the study group versus 23.2% in the control
group (P = 0.03). The implantation rate per transferred embryo was 6.0% in
the study group versus 11.3% in the control group (P = 0.003). This study
shows that embryos with one or more multinucleated blastomeres have a
poorer implantation potential than embryos with mononucleated blastomeres.
Transfer of embryos with multinucleated blastomeres should hence only be
considered when insufficient numbers of embryos with only mononucleated
blastomeres are present.
相似文献
3.
H I Abdalla R J Baber T Leonard A Kirkland A Mitchell M Power E Owen J W Studd 《Human reproduction (Oxford, England)》1989,4(8):927-930
Three-hundred-and-twenty-five patients on an assisted conception programme underwent 378 cycles of oocyte retrieval (OPU) following ovarian stimulation using a GnRH analogue and human menopausal gonadotrophins (HMG), a regimen which allows programmed cycles and delayed oocyte retrieval. Eighteen cycles were excluded (failed OPU in three and failure of fertilization in 15). In 360 cycles, patients completed their treatment with either in-vitro fertilization/embryo transfer (IVF/ET) (116) or gamete intra-Fallopian transfer (GIFT) (244), of which 241 took place at the normal time and 119 were delayed for 24 h or more to avoid weekend operating. The overall pregnancy rate per OPU was 29.5%, with the IVF group being 24.1% and the GIFT group being 32.8%. In the group of patients in whom OPU was delayed, the pregnancy rate was significantly higher in each sub-group than in the corresponding non-delayed sub-group (overall, 37.0 versus 25.7%; IVF/ET, 38.5 versus 16.9%; GIFT, 36.3 versus 31.1%). There was a significantly higher number of oocytes collected, gametes/embryos transferred in the group whose OPU had been delayed. In patients receiving GnRH analogue and HMG for ovarian stimulation, delaying oocyte retrieval is not harmful, may result in an improved outcome and allows OPU to be performed on routine operating lists. This facility, together with the improved pregnancy rates associated with this protocol of ovarian stimulation should improve the cost-effectiveness of assisted conception programmes. 相似文献
4.
Laverge H; De Sutter P; Desmet R; Van der Elst J; Dhont M 《Human reproduction (Oxford, England)》1997,12(10):2263-2266
The use of human serum albumin (HSA) instead of fetal cord serum (FCoS) as
protein supplement highly simplifies the preparation of culture medium for
human in-vitro fertilization (IVF) but whether they are equivalent in
sustaining embryo development is still controversial. We performed a
prospective randomized study of patients undergoing IVF or intracytoplasmic
sperm injection (ICSI) where embryos were cultured in Earle's balanced salt
solution containing either 8% (v/v) FCoS or 0.4% (w/v) HSA as protein
source. Fertilization rates, morphological embryonic quality and pregnancy
rates were compared. A total of 2189 oocytes from 210 cycles were cultured
in medium supplemented with HSA in patient group 1 and 2109 oocytes from
203 cycles in medium supplemented with FCoS in patient group 2. The
fertilization rate, defined as the presence of two nuclei, for
microinjected oocytes was similar in both patient groups (77.4 and 76.7%,
respectively). The fertilization rate for inseminated oocyte-cumulus
complexes was significantly higher in the HSA group than in the FCoS group
(62.9 versus 53.8%, P < 0.025). The embryonic quality was significantly
better after culture in medium supplemented with HSA than with FCoS (13.7
versus 9.9% morphologically excellent embryos, P < 0.001). Implantation
rates per transferred embryo were not significantly different (22.5 versus
18.2%), but there was a significantly higher pregnancy rate per embryo
transfer in the HSA group (45.7 versus 35.9%, P < 0.05, respectively).
Non-evolutive pregnancy rates were significantly different (27.4 and
16.7%). Our data demonstrate that the use of human serum albumin as a
protein supplement for culture medium in human IVF programmes is associated
with improved embryonic quality and significantly higher pregnancy rates.
For this reason as well as the additional benefits of being virus-free and
being purified, HSA is preferable to FCoS for the preparation of culture
media in human IVF.
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5.
Neubourg DD Mangelschots K Van Royen E Vercruyssen M Ryckaert G Valkenburg M Barudy-Vasquez J Gerris J 《Human reproduction (Oxford, England)》2002,17(10):2621-2625
BACKGROUND: The aim of this study was to evaluate the impact of transferring a single top quality embryo in the first IVF/ICSI cycle of patients <38 years old who chose to have one or two embryos transferred. METHODS: A total of 262 patients participated in the study, and 243 transfers were performed: 156 (64%) patients chose the transfer of a single top quality embryo, if available, and two non-top quality embryos if not available; 87 (36%) patients chose to have a double embryo transfer regardless of embryo quality. RESULTS: In the first group an ongoing pregnancy rate of 40% (63/156) with a twin pregnancy rate of 2% (1/63) was achieved. In the second group the ongoing pregnancy rate was 44% (38/87) with 26% (10/38) twin pregnancies. In the patient group with only one embryo transferred, irrespective of the patient's choice, the ongoing pregnancy rate was 43% (54/127) with no twin pregnancies. For the study population as a whole, the ongoing pregnancy rate was 42% (101/243) with 11% (11/101) twins. CONCLUSION: We conclude that the introduction of single embryo transfer in the first IVF/ICSI cycle is highly acceptable in women <38 years old. 相似文献
6.
目的探讨冻融胚胎移植在常规体外受精(IVF)失败后补救卵胞浆内单精子注射(L-ICSI)中的应用价值。方法在12个常规体外受精失败周期中应用ICSI对未受精的MⅡ期卵子进行显微授精,将获得的优质胚胎进行冷冻,再择期行冻融胚胎移植。结果对93个未受精的MⅡ卵子接受L-ICSI,受精63枚,受精率为67.7%(63/93),异常受精3枚(2枚1PN,1枚3PN),57个正常受精卵发生卵裂,卵裂率为95.0%(57/60),优质胚胎率为43.9%(25/57),10例患者冷冻胚胎25枚,其中4例采用程序化冷冻,6例采用玻璃化冷冻。9个患者行冻融胚胎移植,共移植胚胎18枚(其中解冻后胚胎碎裂死亡5枚),其中1个周期因冻融后2个胚胎碎裂放弃移植,2例获得临床妊娠,1例分娩出正常婴儿,1例正在妊娠中,临床妊娠率为22.2%。结论 ICSI可使常规体外受精失败的卵子再受精,冻融胚胎移植可以解决胚胎与子宫内膜不同步的问题,获得相对满意的临床结局,具有一定的应用价值。 相似文献
7.
Staessen C.; Nagy Z.P.; Liu J.; Janssenswillen C.; Camus M.; Devroey P.; van Steirteghem A.C. 《Human reproduction (Oxford, England)》1995,10(12):3305-3312
High incidences of multiple pregnancies, after transferringa maximum of three embryos, were observed after in-vitro fertilization(IVF) treatment. In a randomized study, it was demonstratedthat, after taking into account embryo quality and other positivelyinterfering parameters, an elective transfer of two good qualityembryos does not significantly influence the pregnancy rate.The intracytoplasmic sperm injection (ICSI) technique was successfullydeveloped in the meantime and high incidences of multiple pregnancieswere also obtained after ICSI. The question arose whether afterICSI there was also room for elective double embryo transferin a well-defined patient group. This report covers 1 year of IVF and ICSI treatment and theresults are presented in relation to the number of embryos transferred.The embryo development is similar for zygotes obtained afterIVF and ICSI; for both techniques 63% of the zygotes developto type A-B embryos and 13% to type C embryos. There is alsono difference in the pregnancy rate after ICSI or IVF. Globally,after IVF, 307 out of the 766 double and triple transfers (40.1%)and 317 out of 774 double and triple transfers (40.9%) afterICSI resulted in a positive HCG. After IVF, 73.9% (227) andafter ICSI 76.3% (242) of the pregnancies were evolutive. Neitherwas there any difference between the two techniques as regardsthe implantation rate per transferred embryo. After IVF, 22.8%of the transferred embryos implanted compared with 21.8% afterICSI. When the elective double embryo transfers were compared,no difference was found between IVF and ICSI. After IVF, 102of the 211 elective double transfers (48.1%) resulted in a pregnancyversus 93 out of 225 (41.3%) after ICSI [not significant (NS)].A high implantation rate per transferred embryo (IVF: 33.2%;ICSI: 26.9%, NS) was obtained in this elective double transfercategory, as was also reported in the randomized study. Thesedata confirm the results obtained in our randomized study andthe effectiveness of the elective double embryo transfer forIVF as well as for ICSI. 相似文献
8.
J Gerris D De Neubourg K Mangelschots E Van Royen M Van de Meerssche M Valkenburg 《Human reproduction (Oxford, England)》1999,14(10):2581-2587
A prospective randomized study comparing single embryo transfer with double embryo transfer after in-vitro fertilization or intracytoplasmic sperm injection (IVF/ICSI) was carried out. First, top quality embryo characteristics were delineated by retrospectively analysing embryos resulting in ongoing twins after double embryo transfer. A top quality embryo was characterized by the presence of 4 or 5 blastomeres at day 2 and at least 7 blastomeres on day 3 after insemination, the absence of multinucleated blastomeres and <20% cellular fragments on day 2 and day 3 after fertilization. Using these criteria, a prospective study was conducted in women <34 years of age, who started their first IVF/ICSI cycle. Of 194 eligible patients, 110 agreed to participate of whom 53 produced at least two top quality embryos and were prospectively randomized. In all, 26 single embryo transfers resulted in 17 conceptions, 14 clinical and 10 ongoing pregnancies [implantation rate (IR) = 42.3%; ongoing pregnancy rate (OPR) = 38.5%] with one monozygotic twin; 27 double embryo transfers resulted in 20 ongoing conceptions with six (30%) twins (IR = 48.1%; OPR = 74%). We conclude that by using single embryo transfer and strict embryo criteria, an OPR similar to that in normal fertile couples can be achieved after IVF/ICSI, while limiting the dizygotic twin pregnancy rate to its natural incidence of <1% of all ongoing pregnancies. 相似文献
9.
Terriou P; Giorgetti C; Auquier P; Hans E; Spach JL; Salzmann J; Roulier R 《Human reproduction (Oxford, England)》1997,12(5):1069-1072
The purpose of this study was to retrospectively compare the overall
results and embryo quality in 102 cycles of in-vitro fertilization
(IVF)-embryo transfer using normal frozen donor semen (group D) and 94
cycles of IVF-embryo transfer using husbands' teratozoospermic sperm (group
T). Donor semen was purchased from men with proven fertility and normal
semen parameters. Teratozoospermia was defined in group T as the presence
of <20% of normal spermatozoa in semen on the day of oocyte retrieval.
Exclusion criteria were a sperm count <10 x 10(6)/ml or with <10%
progressive motility. Fertilization rate, transfer rate and number of
transferred embryos per cycle were significantly lower in the
teratozoospermic group (45 vs 72%, 66 vs 96%, 1.7 vs 2.9%, respectively).
Pregnancy rate per cycle was also lower, but not significantly (18 vs 28%).
However, pregnancy rate per transfer, implantation rate per transferred
embryo and take home baby rate were comparable (27 vs 30%, 15 vs 15%, 21 vs
24%, respectively). Similarly, embryo quality in terms of number of embryos
displaying fragmentations or irregular cells, cleavage stages and embryo
scores were comparable. When group T was divided into two subgroups
according to sperm count (group T1: sperm count = 10-20 x 10(6)/ml; group
T2: sperm count >20 x 10(6)/ml), there was no difference between them
with regard to fertilization rate, pregnancy rate or embryo quality. This
study confirms low pregnancy rate per cycle in IVF-embryo transfer using
teratozoospermic semen, but demonstrates for the first time that embryo
quality and viability are not impaired. It is proposed that the poor
pregnancy rate per cycle obtained is due only to the poor fertilization
rate, and to the subsequent limited choice of embryos to be transferred.
相似文献
10.
R M Janssens C B Lambalk R Schats J Schoemaker 《Human reproduction (Oxford, England)》1999,14(10):2497-2498
A case is reported of successful in-vitro fertilization (IVF) and pregnancy in a natural cycle after four previously failed attempts with stimulated cycles. The patient began treatment at the age of 36 years and underwent four stimulated IVF cycles, each time with three embryos of good quality transferred. In one attempt, three cryopreserved embryos were transferred in a natural cycle. The patient failed to conceive. At the age of 38 years, the patient was entered into a natural cycle IVF programme. The patient conceived twice in each of her first two attempts but unfortunately aborted. In her third natural cycle of IVF, again with one oocyte obtained and one embryo transferred, the patient conceived and had a full term gestation. It is concluded that IVF in a natural cycle is a viable option for infertile women with blocked Fallopian tubes who have normal ovulatory menstrual cycles. 相似文献
11.
Devreker F Pogonici E De Maertelaer V Revelard P Van den Bergh M Englert Y 《Human reproduction (Oxford, England)》1999,14(12):3002-3008
Embryo quality evaluated by the embryo morphology is a critical parameter in human in-vitro fertilization (IVF) and embryo transfer. It determines which and how many embryos will be replaced, as pregnancy rates are directly related to number and quality of transferred embryos. This retrospective analysis included 1301 IVF and embryo transfer cycles to identify which factors influenced embryo quality. Embryo quality did not correlate with maternal age, causes of infertility, ovarian stimulation parameters or embryo cohort size. However, the mean score of transferred embryos was significantly higher for patients with more than five embryos compared to fewer than five embryos (P < 0.001), irrespective of maternal age. Patients tended to produce a similar embryo quality from cycle to cycle, r = 0.33 (P < 0.001) for the embryo cohort and r= 0.47 (P < 0.001) for the transferred embryos. Poor embryo morphology probably reflects oocytes with compromised development competence and could be an independent factor of infertility. Furthermore, a large embryo cohort was the main factor increasing the chances of at least one good embryo in the cohort. 相似文献
12.
Tasdemir Murat; Tasdemir Isik; Kodama Hideya; Fukuda June; Tanaka Toshinobu 《Human reproduction (Oxford, England)》1995,10(8):2155-2158
The rational of transferring two instead of three embryos wasstudied through 468 in-vitro fertilization (IVF) treatment cyclesin 287 couples. The quality of 1224 embryos was determined accordingto the fragmentation rate and the morphology as good (A) andpoor (B). The influence of the number of embryos transferred(two or three) on the pregnancy rate when the same quality orcombinations of good and poor quality embryos transferred wasexamined. When only good quality embryos were transferred thepregnancy rates in double (AA) and triple (AAA) embryo transferwere 40.5 (17/42) and 42.9% (30/70) respectively (not significant).When only poor quality embryos were transferred, the pregnancyrates in double (BB) and triple (BBB) embryo transfers were11.0% (11/ 100) and 22.9% (16/70) respectively (P < 0.001).On the other hand, when good and poor quality embryos were transferredtogether as AB in double and as AAB and ABB in triple embryotransfer, the pregnancy rates were 36.8 (14/38) and 39.9% (59/148)respectively (not significant). There was no difference in themiscarriage rate between double and triple embryo transfers;16.7 and 18.1% respectively. The multiple pregnancy rate was14.3% for double embryo transfers and 32.4% for triple embryotransfers (P < 0.001). This study demonstrates that if thereis at least one good quality embryo available for transfer,then double instead of triple embryo transfer will not yielda significantly lower pregnancy rate. The influence of the numberof embryos transferred on the pregnancy rate became significantwhen only poor quality embryos were transferred. In conclusion,as long as at least one good quality embryo is available fortransfer, we may consider the transfer of double instead oftriple embryos. 相似文献
13.
The contribution of embryo cryopreservation to in-vitro fertilization/gamete intra-Fallopian transfer: 8 years experience 总被引:6,自引:6,他引:0
Wang X.J.; Ledger W.; Payne D.; Jeffrey R.; Matthews C.D. 《Human reproduction (Oxford, England)》1994,9(1):103-109
In this paper, the authors summarized their experience withembryo cryopreservation over an 8-year period. The results,therefore, reflect the long-term benefit of embryo cryo-preservationto the overall in-vitro fertilization/gamete intra-Fallopiantransfer (IVF/GIFT) programme and to the women who had embryoscryopreserved. The stable survival rate of thawed embryos andpregnancy rate, especially over the past 4 years, suggests thatthe results can reliably be used to evaluate the efficacy ofthe embryo cryopreservation programme. The ongoing pregnancyrate of frozen/thawed embryo transfer is 10.9%, comparable withthe ongoing pregnancy rate of fresh IVF/embryo transfer in ourunit over the same period. In addition to those factors knownto affect the pregnancy rate in fresh IVF/GIFT cycles, suchas age of the recipients and number of embryos transferred,the major factor affecting the efficacy of the cryopreservationprogramme is the number of oocytes retrieved in the initialstimulation cycle, and the number of embryos available for cryopreservation.The storage time of cryopreserved embryos will also have a significanteffect on the realization of the total potential of embryo cryopreservation.Overall the contribution of cryopreservation to our IVF/GIFTprogramme is substantial, increasing pregnancy rate by 4%, whilethe greater net benefit, of course, is for the women who hadembryos cryopreserved (pregnancy rate increased by 7%), especiallyfor those who returned for frozen/thawed embryo transfer cycles(pregnancy rate increased by 11%). 相似文献
14.
Genital tract abnormalities and adverse pregnancy outcome are well known in women exposed in utero to diethylstilboestrol (DES). Data about adverse reproductive performance in women exposed to DES have been published, including controversial reports of menstrual dysfunction, poor responses after ovarian stimulation, oocyte maturation and fertilization abnormalities. We compared oocyte quality, in-vitro fertilization results and embryo quality for women exposed in utero to DES with a control group. Between 1989 and 1996, 56 DES-exposed women who had 125 in-vitro fertilization (IVF) attempts were retrospectively compared to a control group of 45 women with tubal disease, who underwent 73 IVF attempts. Couples suffering from male infertility were excluded. The parameters compared were oocyte quality (maturation abnormalities, immature oocyte, mature oocyte), fertilization and cleavage rate (per treated and metaphase II oocytes), and embryo quality (number and grade). We found no significant difference in oocyte maturational status, fertilization rates, cleavage rates, embryo quality and development between DES-exposed subjects and control subjects. These results suggest that in-utero exposure to DES has no significant influence on oocyte quality and fertilization ability as judged during IVF attempts. 相似文献
15.
E Van Royen K Mangelschots D De Neubourg M Valkenburg M Van de Meerssche G Ryckaert W Eestermans J Gerris 《Human reproduction (Oxford, England)》1999,14(9):2345-2349
In most in-vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) programmes approximately one ongoing pregnancy in three is multiple. The need to characterize embryos with optimal implantation potential is obvious. We retrospectively examined all of 23 double transfers resulting in ongoing twins, occurring between January 1, 1996 and May 19, 1997. Characteristics of these top quality embryos were absence of multinucleated blastomeres, four or five blastomeres on day 2, seven or more cells on day 3, and =20% anucleated fragments. In a subsequent series of 400 IVF/ICSI cycles (out of which 372 were selected for embryo transfer) from May 20, 1997 to July 31, 1998, only women <38 years of age had multiple pregnancies: after 221 transfers of two embryos, 45/116 (39%) were multiple, and after 77 transfers of >2 embryos, 11/31 (35%) were multiple. We applied our top quality criteria to the 221 double transfers: 106 transfers with two top embryos resulted in 65 (63%) ongoing pregnancies with 37 (57%) twins, 65 transfers with one top embryo in 38 (58%) ongoing pregnancies with eight (21%) twins. In the group without top embryos, 12/52 (23%) ongoing singletons occurred, with no twins. The corresponding ongoing implantation rates were 49, 35 and 12%. This analysis suggests that single embryo transfer with an acceptable pregnancy rate might be considered if a top quality embryo is available. 相似文献
16.
Frozen embryo transfers: implications of clinical and embryological factors on the pregnancy outcome
Salumets A Suikkari AM Mäkinen S Karro H Roos A Tuuri T 《Human reproduction (Oxford, England)》2006,21(9):2368-2374
BACKGROUND: Frozen embryo transfers are characterized by impaired pregnancy outcome and increased incidence of pregnancy loss as compared with fresh IVF/ICSI embryo transfers. In this study, we performed a retrospective analysis of clinical and embryological factors that potentially influence the outcome of frozen embryo transfer. METHODS: We reviewed the outcome of 1242 frozen embryo transfers with respect to the age of the woman, the method of fertilization, embryo quality before and after freezing and the number of embryos transferred. RESULTS AND CONCLUSIONS: The pregnancy (positive hCG) and clinical pregnancy rates were 25.8 and 21.1%, respectively. A total of 107 (33.3%) of the 321 pregnancies identified by a positive hCG test miscarried either before (18.4%) or after (15%) the clinical recognition of gestational sac(s). The delivery rate for the frozen embryo transfers analysed was 17.2%. Our data revealed that the delivery rate after frozen embryo transfer was dependent on both the woman's age and the quality of embryos transferred, at the same time being unaffected by IVF/ICSI treatment. In addition, the increased woman's age at IVF/ICSI treatment was identified as the only parameter elevating the biochemical pregnancy rate, whereas the clinical abortion rate was found to be unrelated to the clinical or embryological parameters studied. 相似文献
17.
目的探讨不同氧分压环境下(20%02&5%O2)体外受精一胚胎移植中胚胎发育潜能与临床结局关系。方法选择2012年9月至2012年11月年在郑大三附院生殖医学中心行助孕治疗的280个周期,分别为IVF(n=200),ICSI(n=80)。随机分组:研究组(5%氧分压)和对照组(20%氧分压),对比两组间正常受精率、卵裂率、优质胚胎率、生化妊娠率、临床妊娠率和着床率。结果实验组与对照组正常受精率、卵裂率、优质胚胎率、生化妊娠率、临床妊娠率和着床率无统计学差异。结论人类早期胚胎在低氧环境下进行体外培养是有利的,可以获得更好结局。 相似文献
18.
V Vlaisavljevi? B Kovacic M Reljic V G Lovrec M C Sajko 《Human reproduction (Oxford, England)》2001,16(11):2379-2383
BACKGROUND: The aim of the study was to test the influence of 2- and 5-day cultivation of a single oocyte on the pregnancy rate in a non-stimulated cycle. METHODS: A retrospective chart review of 391 consecutive patients undergoing IVF and intracytoplasmic sperm injection in unstimulated cycles was performed. The embryos were kept in MediCult universal IVF medium for day 2 transfers and in BlastAssist System for day 5 transfers. RESULTS: The oocyte recovery rate in the group for 2-day cultivation and in the group for 5-day cultivation was similar, being 79.4 (162/204) and 83.6% (154/187) respectively. The same is true of the fertilization rate (73.8 versus 77.7%). The blastulation rate was 52.8%. The embryo transfer rate per cycle was higher when day 2 embryos were transferred: 64.8% (105/162) compared with 35.7% (55/154) if blastocyst-stage embryos were transferred. The pregnancy rate per transferred embryo was higher when a blastocyst was transferred (40.0%) instead of a day 2 embryo (23.8%). CONCLUSION: The expected pregnancy rate calculated per embryo available on day 2 of cultivation was similar in both groups (23.8 versus 22.2%) and it was not affected by oocyte culture to the blastocyst stage. 相似文献
19.
Co-culture techniques using fetal bovine uterine fibroblasts or bovine oviductal epithelial cells have improved embryonic development prior to replacement in humans. In initial co-culture trials, embryo development and implantation rates increased after just 1 day in culture. The most overt characteristics noted following co-culture were improved blastomere development and characteristics, reduced fragmentation, and the appearance of swollen blastomeres. In addition, an increase in the incidence of zona thickness variation was detected. Improved development of polyspermic and supernumerary embryos to the blastocyst stage was noted in initial trials. Retrospective analysis indicated that certain patient subgroups benefit the most from co-culture. As a result, co-culture is now applied routinely to patients that have previously failed attempts at in-vitro fertilization (IVF) and/or have endocrine imbalances such as polycystic ovarian syndrome and elevated day 3 concentrations of follicle stimulating hormone (FSH). The use of co-culture prior to or following cryopreservation has also proven to be beneficial to human embryos. The proposed beneficial mechanisms thought to improve embryonic development include a secretory and/or a scavenging role. Evidence describing the postulated benefits is discussed. 相似文献
20.
Permanent impairment of embryo development by hydrosalpinges 总被引:6,自引:9,他引:6
Recent reports suggest a deleterious effect of hydrosalpinges on pregnancy
outcome for in-vitro fertilization (IVF) and improvement following surgical
treatment. We compared the effect of hydrosalpinx on pregnancy outcome in
286 patients having 348 IVF cycles and followed the development of
untransferred embryos for 7 days to determine if hydrosalpinges affect
oocyte quality or embryo development. The delivery rate per retrieval was
significantly lower for patients with hydrosalpinx, but was restored by
surgical treatment to that of patients without hydrosalpinx. However, the
implantation rate per embryo transferred and normal blastulation of
untransferred embryos, which were significantly decreased in patients with
hydrosalpinx, and growth arrest and degeneration of untransferred embryos,
which were significantly increased compared to patients without
hydrosalpinx, were not restored by surgical treatment of hydrosalpinges. We
conclude that surgical treatment of hydrosalpinges decreases early
pregnancy loss and improves pregnancy outcome, possibly by diminishing
reversible deleterious effects exerted on the endometrium. As we have seen
in our laboratory, hydrosalpinges may have a permanent negative influence
on ovarian function, follicular development and oocyte quality since
implantation of transferred embryos and normal blastulation of
untransferred embryos remain low, and in-vitro growth arrest and
degeneration remain high despite surgical treatment of hydrosalpinges.
相似文献