Analgesic effects of different acupoint stimulation frequencies in humans

Transcutaneous electrical acupoint stimulation (TEAS) provides a convenient and standardized technique for pain treatment. The cold-pressor test is a simple and reliable model in humans for the induction of tonic pain. In this controlled study, the effects of TEAS on cold pressor-induced pain were evaluated in 22 healthy human subjects. Electrical stimulation at 4 Hz and 32 Hz was applied to He-Gu (LI 4) and Nui-Guan (P 6) acupoints for 15 minutes. Pain score ratings were evaluated at four time points from 30-170 seconds during the cold-pressor test. We observed an analgesic effect at both 4 Hz and 32 Hz of stimulation, and pain score rating reductions were statistically significant compared to control (p < 0.01). Our data support the efficacy of TEAS analgesia. However, there was no significant difference between pain scores at 4 Hz and 32 Hz stimulation.     

不同频率电针对家兔胃电节律紊乱调整效应研究

目的:观察不同频率电针对红霉素诱导的家兔胃电节律亢进的调整效应,为针灸临床选择合适频率参数提供参考依据。方法:将40只家兔做耳缘静脉注射红霉素复制胃电节律紊乱模型后,随机分为模型组、3Hz电针组、20Hz电针组、100Hz电针组,后3组分别用3Hz、20Hz、100Hz3种频率电针刺激“足三里”穴位,应用IEGG谱分析仪检测不同时间段的主频功率(PDP)和总功率(PTP)。结果:红霉素可使家兔胃电PDP和PTP明显增高(P<0·001);3种频率电针对家兔胃电PDP及PTP均有显著的降低作用(P<0·05),对PDP的影响3种频率之间差异无显著性意义(P>0·05);对PTP的影响20Hz电针疗效明显优于3Hz电针组(P<0·05),但与100Hz电针组比较差异无显著意义(P>0·05)。结论:3种频率电针对家兔胃电节律紊乱有显著的调整作用,可使亢进的胃肠运动降低,而且不同频率电针对胃电的调整效应不同。     

氧化苦参碱的镇痛作用

目的:探讨在不同的疼痛模型中,氧化苦参碱(Oxymatrine,OMT)的镇痛效果。方法:昆明小鼠随机分为空白组(生理盐水组),吗啡组,纳洛酮组及给药组,采用醋酸扭体法、热板法,观察给药后小鼠扭体次数(WT)、舔后足潜伏期(HPPT),同时注射纳洛酮观察其对氧化苦参碱镇痛作用的影响;采用部分结扎坐骨神经法(PSNL)建立模型,用up and down方法分别测结扎前、结扎后第3,7,14,21天小鼠手术侧后足底给药前后足底机械缩足反应阈值(MWT),观察氧化苦参碱(150 mg/kg)对神经性疼痛的影响。结果:氧化苦参碱(25～200 mg/kg)各剂量依赖性地减少醋酸所致的小鼠扭体次数,提高小鼠热刺激引起的痛阈,且以上镇痛作用不能被纳洛酮所拮抗;氧化苦参碱(150 mg/kg)还能提高部分结扎坐骨神经法手术组小鼠的机械缩足反应阈值。结论:氧化苦参碱对物理性疼痛、化学刺激性疼痛及神经性疼痛均有抑制作用。     

Antinociceptive effect of Teucrium polium leaf extract in the diabetic rat formalin test

This study was designed to evaluate the analgesic effect of Teucrium polium leaf extract in the diabetic rat formalin test. For this purpose, streptozotocin (STZ)-diabetic rats received intraperitoneal injection of this extract (100 and 200 mg/kg per day) for a period of 2 weeks. It was found out that Teucrium polium-treated diabetic rats exhibited a lower nociceptive score as compared to untreated diabetics. The results may suggest therapeutic potential of Teucrium polium extract for the treatment of diabetic hyperalgesia.     

不同频率电针刺激对脑出血大鼠血肿脑组织脑红蛋白及NLRP3信号通路的影响

目的 研究不同频率电针刺激对脑出血大鼠血肿脑组织中脑红蛋白(Ngb)及NL-RP3信号通路相关指标的影响.方法 将90只健康Wistar大鼠随机分为正常组、假手术组、模型组、电针1组、电针2组、电针3组,每组15只.模型组和电针各组以大鼠尾状核自体动脉血注入法复制脑出血模型.大鼠造模苏醒后,电针各组选取百会、水沟、双侧...     

Analgesic effect of different moxibustion durations in rheumatoid arthritis rats

OBJECTIVE： To observe the influence of different moxibustion durations on hypothalamic pro-opi- omelanocortin（POMC） and prodynorphin（PDYN） mRNA expressions and plasma β-endorphin（β-EP） content in rheumatoid arthritis（RA） rats, to under- stand the mechanism of moxibustion analgesia and its dose-effect relationship. METHODS： Twelve male Wistar rats were randomly selected from 48 male Wistar rats as a normal con- trol group. The RA model was created by raising rats in a windy（blowing with electric fan）, cold（6 ℃ ±2 ℃）, and wet（80%-90% humidity） environ- ment for 20 days, 12 h each day. This was followed byinjectionofFreund＇scompleteadjuvant（0.15 mL） into the ankle. Then, rats were randomly divided in- to a model group, moxibustion group Ⅰ, and moxi- bustion group Ⅱ, with 12 rats in each group. In moxibustion groups Ⅰ and Ⅱ, moxibustion was given at Shenshu（BL 23） and Zusanli（ST 36） for 20 and 40 min, respectively, once daily for 15 days. Hy- pothalamic POMC and PDYN mRNA expression lev- els and plasma β-EP content were determined.RESULTS： Compared with the normal group, the pressure pain threshold decreased, while the hypo- thalamic POMC and PDYN mRNA expression levels and plasma β-EP content increased in the moxibus- tion groups（P0.01）. Compared with the model group, the pressure pain threshold, hypothalamic POMC and PDYN mRNA expression levels and plas- ma β-EP content in the moxibustion groups in- creased significantly（P0.01）. Compared the moxi- bustion groupⅠ, the pain threshold, hypothalamic POMC and PDYN mRNA expression levels and plas- ma β-EP content in moxibustion groupⅡsignifi- cantly increased（P0.01）. CONCLUSION： Moxibustion has an analgesic effect and increases hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content inRArats.Theanalgesiceffectinmoxibustiongroup ⅡisbetterthanthatinmoxibustiongroupⅠ.     

Analgesic effect of different moxibustion durations in rheumatoid arthritis rats

Objective

To observe the influence of different moxibustion durations on hypothalamic pro-opiomelanocortin (POMC) and prodynorphin (PDYN) mRNA expressions and plasma β-endorphin (β-EP) content in rheumatoid arthritis (RA) rats, to understand the mechanism of moxibustion analgesia and its dose-effect relationship.

Methods

Twelve male Wistar rats were randomly selected from 48 male Wistar rats as a normal control group. The RA model was created by raising rats in a windy (blowing with electric fan), cold (6°C ± 2°C), and wet (80%-90% humidity) environment for 20 days, 12 h each day. This was followed by injection of Freund's complete adjuvant (0.15 mL) into the ankle. Then, rats were randomly divided into a model group, moxibustion group I, and moxibustion group II, with 12 rats in each group. In moxibustion groups I and II, moxibustion was given at Shenshu (BL 23) and Zusanli (ST 36) for 20 and 40 min, respectively, once daily for 15 days. Hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content were determined.

Results

Compared with the normal group, the pressure pain threshold decreased, while the hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content increased in the moxibustion groups (P<0.01). Compared with the model group, the pressure pain threshold, hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content in the moxibustion groups increased significantly (P<0.01). Compared the moxibustion group I, the pain threshold, hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content in moxibustion group II significantly increased (P<0.01).

Conclusion

Moxibustion has an analgesic effect and increases hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content in RA rats. The analgesic effect in moxibustion group II is better than that in moxibustion group I.     

经皮穴位电刺激治疗不同时期肩周炎疗效观察

目的:观察经皮穴位电刺激对不同时期肩周炎的治疗作用及与电针治疗的效应差异。方法:将360例不同时期肩周炎患者分成经皮穴位电刺激治疗组(186例)和电针治疗组(174例),各组采用相同经穴分别治疗。结果:经皮穴位电刺激对粘连前期和粘连期肩周炎的总有效率分别达到96.6%和96.9%,电针刺激对粘连前期和粘连期肩周炎的总有效率分别达到93.5%和97.9%,两组比较差异无显著性意义(P>0.05);经皮穴位电刺激疗法不仅能有效止痛,对粘连期的肩关节活动障碍也有明显的改善作用。结论:经皮穴位电刺激为治疗肩周炎有效、简便的疗法。     

论穴位注射与外周神经损伤的关系

目的:为增强穴位注射安全性和提高临床疗效提供依据。方法:以穴位注射、神经损伤为关键词,应用计算机检索CNKI中文期刊全文数据库内的相关文章进行分析研究。结果:大多数临床报道集中在运用穴位注射疗法治疗外伤、产伤所引起的神经损伤方面。研究表明穴位注射导致的外周神经损伤可分为3级;损伤机理可分为3类;损伤原因有取穴姿势不当、针刺角度深度有误和药物选择不当两种。结论:有必要对穴位注射疗法的操作进行规范,以避免外周神经的损伤。     

针刺复合麻醉对不同痛阈患者的镇痛效果

目的:观察针刺复合麻醉对不同痛阈患者的镇痛效果.方法:将700例准备行经阴道超声引导取卵术接受体外受精和胚胎移植的不孕症患者随机分为针刺复合麻醉组(347例)和单纯度冷丁组(353例),针刺复合麻醉组于取卵前1日预针刺前30 min测量痛阂,单纯度冷丁组于取卵手术前30 min注射度冷丁前测量痛阈,取卵术前30 min两组分别接受电针(穴取肾俞、次(骨翏)、百会、三阴交等)结合肌肉注射度冷丁和单纯肌肉注射度冷丁镇痛.结果:痛阈为1 mA、1.5 mA和2 mA的患者,针刺复合麻醉组的疼痛积分显著低于单纯度冷丁组(P＜0.01,P＜0.05),对于痛阈为0.5 mA、2.5 mA、3 mA的患者,两组疼痛积分差异无统计学意义(均P＞0.05).结论:针刺复合麻醉对于痛阈处于中间段的患者镇痛效果最佳.     

经皮穴位电刺激复合TCI在全麻中镇痛效应及对心血管系统的影响

目的：探讨经皮穴位电刺激复合靶控输注（TCI）在全麻中镇痛效应及对心血管系统的影响。方法：选择择期行乳腺手术的患者60例，随机分为2组，经皮穴位电刺激复合TCI组（简称复合组）和单纯TCI组（简称TCI组），每组30例。复合组患者先行经皮穴位电刺激麻醉诱导30min，然后行TCI静脉全身麻醉，经皮穴位电刺激持续刺激至手术结束；TCI组单纯行TCI静脉全身麻醉。通过对全麻药血浆靶控浓度的影响，评价经皮穴位电刺激的镇痛效应。同时观察经皮穴位电刺激在术中对患者心血管系统的影响。结果：复合组及TCI组患者术中平均所需的丙泊酚量分别为226．67mg及272．22mg，平均所需的芬太尼量分别为0．11mg及0．14mg，组间差异具有显著性或非常显著性意义（P〈0．05，P〈0．01）；复合组切皮前后心率、血压的波动均较TCI组小，差异具有显著性意义（P〈0．05）；复合组患者苏醒时间明显缩短。结论：经皮穴位电刺激在术中具有一定的麻醉镇痛作用，可相对减少17％丙泊酚用量及14％芬太尼用量；同时，经皮穴位电刺激可以减轻手术切皮对患者所致的心血管系统的应激反应，并可加快患者的苏醒。     

穴位电刺激用于人工流产镇痛的临床研究

目的探讨穴位电刺激在人工流产过程中的镇痛作用及预防或降低人工流产综合征发生的有效性。方法将120例行人工流产术的患者随机分为2组,治疗组60例应用穴位电刺激预刺激5min后改用治疗频率,同时行人工流产术。对照组60例则用传统方法行人工流产术。分别观察2组患者术中的血压(收缩压)、心率及术中、术后恶心和呕吐情况,并采用视觉模拟评分法评估患者的疼痛程度。结果2组比较,对照组心率明显低于治疗组(P<0.05),收缩压变化则无统计学意义(P>0.05),治疗组术中及术后恶心呕吐发生率明显低于对照组(P<0.05),且治疗组疼痛程度较对照组明显减轻(P<0.05)。结论穴位电刺激用于人工流产镇痛效果好,能有效降低和减轻人工流产综合征的发生率,具有安全、经济、简单的优势,值得大力推广。     

不同针刺频次对脑梗死恢复期患者疗效的影响

目的:观察每天针刺1次和每天针刺2次治疗脑梗死恢复期患者的效应差异.方法:将97例患者随机分为观察1组(50例)、观察2组(47例).在采取相同中药、西药治疗的基础上,两组均采用电针治疗,穴取极泉、曲池、合谷、环跳等,观察1组每天针刺2次,观察2组每天1次,30天后统计两组疗效.结果:对肢体活动不利症状的改善,观察1组总有效率为94.0%,优于观察2组的78.7%(P＜0.05);在治疗饮水反呛、吞咽困难、神智欠清及语言不利方面,两组疗效相当(均P＞0.05),但观察1组有优于观察2组的趋势.结论:针灸每日2次治疗脑梗死恢复期疗效优于每日1次.     

直流感应电定向透穴疗法治疗特发性面神经炎临床研究

目的 评价直流感应电定向透穴疗法治疗特发性面神经炎的疗效.方法 将符合入选标准的65例特发性面神经炎患者按随机数字表法分为治疗组33例和对照组32例,对照组采用西医常规疗法治疗,治疗组在对照组基础上配合地仓透颊车、阳白透太阳直流感应单向脉冲波定向透穴治疗.2组均治疗3周.分别于治疗前后行House-Brackmann面神经功能分级评估及肌电图瞬目反射检测,评价患者临床疗效及面神经功能的改善情况.结果 治疗组总有效率为66.7%(22/33)、对照组为43.8%(14/32),2组比较差异有统计学意义(U=2.175,P=0.013).治疗后,治疗组House-Brackmann面神经功能Ⅰ级15例、Ⅱ级4例、Ⅲ级3例,对照组Ⅰ级5例、Ⅱ级7例、Ⅲ级2例,2组比较差异有统计学意义(U=2.069,P=0.018);治疗组瞬目反射R1、R2波检出率为87.9%(29/33)、对照组为81.3%(26/32),2组比较差异有统计学意义(U=2.135,P=0.016).治疗后,治疗组瞬目反射R1波[(12.28±2.55)ms比(13.84±3.07)ms,t=2.227]、R2波[(32.28±7.08)ms比(35.95±5.11)ms,t=2.396]潜伏期低于对照组(P值分别为0.030、0.020).结论 直流感应电定向透穴疗法可缩短特发性面神经炎患者瞬目反射R1、R2波,提高临床疗效.     

低频电刺激和强制性训练对中风患者偏身感觉障碍的疗效影响

目的：探索中风患者通过电刺激健患侧肢体感觉刺激的对比,和强制性训练对患侧肢体功能的影响。方法：选择脑卒中存在偏身感觉障碍患者40例,排除意识丧失和严重的认知功能障碍者,随机分成康复组和对照组,每组20例。康复组给予强制性训练和电刺激;对照组给予一般康复训练,采用Fugl—Meyer四肢感觉评定进行比较。结果：康复组在21天治疗后,感觉功能评分、运动功能和日常生活活动功能评分均高于对照组,两组间差异均有统计学意义（P〈0．01）。结论：低频电刺激健患侧肢体争强制性训练能有效改善中风患者的偏身感觉障碍,促进运动功能的恢复,提高生活质量,是临床值得推广的治疗方法。     

不同频率电针刺激对实验性肥胖大鼠减肥效应的影响

目的:探讨不同频率电针刺激对减肥的效应差异。方法:将实验大鼠随机分为对照组、模型组、针刺1组(30Hz)、针刺2组(100Hz),穴取"后三里""三阴交""关元""中脘"。比较治疗前后各组大鼠体重指数(Lees指数)、左肾周脂肪重量和脂肪细胞体积、血脂、胰岛素、瘦素的变化。结果:两针刺组与模型组比较,其体重指数、血脂、左肾周脂肪湿重及脂肪细胞体积均有明显改变(P<0.01),且针刺2组比针刺1组变化程度更高(P<0.05);两针刺组与模型组比较,血清瘦素水平降低(P<0.01),血清胰岛素水平也趋于降低(P<0.05),但针刺1组与针刺2组比较,差异无显著意义(P>0.05)。结论:不同频率电针刺激对肥胖大鼠的脂代谢有不同影响,100Hz电针刺激治疗肥胖症较之30Hz效果更好。     

电针、药物联合重复经颅磁刺激治疗焦虑症的临床疗效观察

[目的]观察电针结合药物联合重复经颅磁刺激治疗焦虑症的疗效。[方法]将84例焦虑症患者随机分为针药经颅磁刺激联合治疗组和对照组,联合治疗组采用电针结合小剂量抗焦虑药配合重复经颅磁刺激治疗,对照组采用电针结合药物治疗,同时给予假性重复经颅磁刺激。将两组的疗效进行对比观察。[结果]两组治疗前后总有效率比较有显著性差异(P<0.05),而且量表评分具有显著性差异,联合治疗组疗效明显优于对照组。[结论]电针结合小剂量抗焦虑药联合经颅磁刺激治疗焦虑症较单纯针药结合治疗疗效更好,肯定了经颅磁刺激对本病的辅助疗效,进一步拓宽了经颅磁刺激的临床应用范围;值得临床加以推广并深入研究。     

功能性电刺激治疗周围神经损伤的疗效分析

目的：观察功能性电刺激（FES）治疗周围神经损伤的临床疗效。方法：采用神经肌体仪-低频脉冲治疗仪对32例周围神经损伤患者行FES治疗,分别于治疗前和疗程结束后予感觉功能评定（MS）、运动功能评定（SS）及肌电图（EMG）检查,比较受损神经运动神经传导速度（MCV）、感觉神经传导速度（SCV）的变化及受损神经所支配肌肉EMG变化。结果：经治疗,26例患者神经功能恢复至S3M3以上,有效率78.12%。神经电生理研究显示,治疗后15例患者出现再生电位,46.87%;治疗后MCV平均值大于治疗前,P〈0.05,比较具有显著差异。结论：FES治疗使周围神经损伤患者的感觉、运动功能有显著提高。FES为周围神经损伤后促进神经再生及肢体功能重建提供了一个有效方式。