高效液相色谱法测定当归中阿魏酸含量方法的探讨

目的 :探讨阿魏酸稳定性及高效液相色谱法测定当归中阿魏酸含量的方法。方法 :以甲酸∶水 (5∶95 )为阿魏酸对照品的溶剂 ,甲酸∶甲醇 (5∶95 )为样品的溶剂 ,固定相为shim packCLC ODS ,流动相为 0 2 %甲醇乙腈∶0 1%磷酸 0 1%三乙胺水溶液(2 0∶80 ) ,检测波长为 3 2 4nm ,流速为 1ml·min-1。结果 :阿魏酸在 0 70 4～ 17 6mg·L-1范围内线性良好 (r =0 9998,n =5 ) ,平均加样回收率为 99 3 3 % ,RSD为 0 60 % (n =6)。结论 :高效液相色谱法简便快速 ,可用于当归药材的含量测定     

高效液相色谱法测定氟康唑软膏的含量

目的 :研究高效液相色谱法测定氟康唑软膏含量的方法。方法 :色谱柱 4× 15 0mm ;流动相为甲醇 -水 (5 0∶5 0 ) ;检测波长为 2 6 1nm ;流速为恒速 1ml·min-1。结果 :氟康唑软膏在 0 0 6～ 0 30mg·ml-1线性范围关系良好。得回归方程为C =7 2 95× 10 -7A +1 76× 10 -2 ,γ =0 9998,回收率为 99 6 % ,RSD =0 73%。结论 :方法快速、准确 ,可作为本品的质控标准。     

吸收度线性组合法测定硝酸毛果芸香碱滴眼液含量

目的 :建立吸收度线性组合法测定硝酸毛果芸香碱滴眼液含量的方法。方法 :分别在 2 0 7、2 2 5及 2 5 6nm波长处测定硝酸毛果芸香碱和尼泊金乙酯的吸收度 ,并通过单测定和线性组合 ,分别计算两者的含量。结果 :硝酸毛果芸香碱线性范围为 1 0～ 5 0 μg·ml-1 ,回收率为 99 1 4 %。RSD =0 36 % ;尼泊金乙酯线性范围为 1～ 6 μg·ml-1 ,回收率为 99 31 %。RSD =0 4 6 %。结论 :此法操作简便 ,结果满意     

反相高效液相色谱法测定盐酸西替利嗪的血药浓度

目的　建立测定人血清中西替利嗪浓度的反相高效液相色谱法。方法　以羟嗪为内标 ,样品用乙酸乙酯提取两次。提取液用氮气吹干 ,残留物用 1. 7%磷酸复溶后进样分析。采用Shim packCLC ODS柱 (15 0mm× 6mm ,i d ,5 μm) ,0 0 2mol·L-1磷酸二氢钠—甲醇 (40∶6 0 ,V/V)为流动相 ,流速 1 2ml·min-1;检测波长 2 2 9nm。结果　在 2 0～ 10 0 0 μg·L-1范围内 ,西替利嗪血药浓度与色谱峰高比线性关系良好 ,r =0 . 9999(n =7,P <0 . 0 0 1) ,最低检测限为 4 μg·L-1。平均回收率为 99. 80 % ,日内RSD≤ 4 .83% (n =4 ) ,日间RSD≤ 4 . 93% (n =5 )。结论　本方法简便、灵敏、结果准确、重现性好 ,适用于西替利嗪药代动力学研究和临床血药浓度测定。     

HPLC法测定胃达苏胶囊中柚皮苷的含量

目的:用HPLC法测定样品中柚皮苷的含量。方法:采用Agilent C18色谱柱(5um,250mm×4·6mm),流动相:乙腈-0·1%磷酸溶液(20:80),检测波长:283nm,结果:柚皮苷的平均回收率99·3%(n=9),RSD=1·2%,线性范围为0·04908-1·227μg(γ=1·0)。结论:方法快速,准确,灵敏,简便易行。     

后程加速超分割放射治疗加同步化疗治疗食管癌

目的 :探讨食管癌后程加速超分割放射治疗加顺铂、氟尿嘧啶同步化疗的疗效和毒性 .方法 :95例食管癌患者随机分成后程加速超分割放射治疗加顺铂、氟尿嘧啶同步化疗组 (综合组 )和后程加速超分割放射治疗组 (后超组 ) .后超组 (4 7例 )前 2 / 3疗程为常规分割 (2Gy/次 ,5次·wk-1 )照射 36～ 4 0Gy后缩野改加速超分割 (1 .5Gy/次 ,2 次·d-1 )照射 2 7～ 30Gy .综合组 (4 8例 )在后超组的基础上静脉内输注顺铂 2 0mg·d-1 ,氟尿嘧啶 5 0 0mg·d-1 ,连用 5d为 1周期 .分别在第 1 ,4周实施 ,共 2个周期 .结果 :1 ,2和 3a生存率 ,综合组分别为 83.3% ,6 4 .6 %和 5 6 .3% ,后超组分别为76 .5 % ,5 1 .1 %和 34.0 % (χ2 =4 .1 6 4 ,P =0 .0 4 1 3) .1 ,2和 3a局部控制率 ,综合组分别为 87.5 % ,77.1 %和 6 6 .7% ;后超组分别为 80 .9% ,6 1 .7%和 4 2 .5 % (χ2 =5 .4 1 9,P =0 .0 2 0 ) .综合组Ⅰ～Ⅲ度急性放射性气管炎发生率分别为 2 0 .8% ,1 2 .5 % ,4 .2 % ;后超组分别为 1 9.2 % ,1 0 .6 % ,2 .1 % ,两组差异无显著性意义 (χ2 =0 .2 0 6 ,P >0 .0 5 ) .综合组Ⅰ～Ⅳ度急性放射性食管炎的发生率分别为 2 9.2 % ,35 .4 % ,8.3%和 2 .1 % ;后超组分别为 2 5 .5 % ,31 .9% ,6 .4 %和 0 .两组差异无显著     

反相高效液相色谱法测定依诺沙星胶囊的血药浓度

目的　建立测定人血清中依诺沙星的浓度高效液相色谱法。方法　选用Shim packCLC ODS柱 (15 0mm× 6mm ,i d ,5 μm) ,0 .1mol L- 1 柠檬酸—甲醇 (78∶2 2 )为流动相 ,流速 1.0ml·min- 1 ,室温 ,检测波长 2 6 6nm ,灵敏度 0 .0 4AUFS。结果　在 0 .0 5～ 10 .0 μg·ml- 1 范围内血药浓度与色谱峰高线性关系良好 ,r=0 .9999(n =7,P <0 .0 0 1) ,平均回收率为 99.83% ,日内RSD≤ 2 .5 4 % (n =3) ,日间RSD≤ 4 .13% (n =5 )。结论　本法简便、灵敏、经济、重现性好 ,可作为依诺沙星药代动力学研究时血药浓度的测定方法。     

高效液相色谱法测定鼠脑组织中5-氟尿嘧啶浓度

目的　建立高效液相色谱法 (HPLC法 )测定鼠脑组织中 5 氟尿嘧啶 ( 5 FU)浓度的方法。方法　脑组织匀浆经乙酸乙酯萃取 ,萃取液挥干 ,残渣经流动相溶解后进行HPLC测定 ,检测波长为 2 70nm。结果　标准曲线方程为 :y =1 0 482 3x -0 1785 43 ,相关系数r =0 9997,线性范围 :0 2 0～ 2 5 0 μg·ml-1,最低定量浓度为 0 2 0 μg·ml-1,日内及日间RSD分别为 0 78%～ 2 73 %和 1 2 4%～ 4 17% ,三种不同浓度的回收率为 99 0 8%～ 10 2 4%。结论　本方法简便、可靠 ,灵敏度高 ,适用于 5 FU在大鼠脑组织内的检测和药代动力学研究。     

反相高效液相色谱法测定克林霉素磷酸酯片的含量

目的　用离子抑制反相色谱法测定克林霉素磷酸酯片中克林霉素的含量。方法　采用C18柱 ,流动相为磷酸二氢钾(KH2 PO4 0 1mol·L-1)—乙腈 (775∶2 2 5 ) ,流速 1 0ml·min-1,柱温为室温 ,进样量 2 0 μl,检测波长 2 10nm ,工作曲线法定量。结果　方法的精密度用平均RSD表示为 1 6% ,测定克林霉素线性范围为 0 0 4～ 1 0 0 g·L-1,平均回收率为 (99 5±1 5 5 ) %。结论　该法简单、快速、准确。可用于克林霉素磷酸酯片中克林霉素的含量测定     

99mTc-HL91肺显像在肺部恶性肿瘤诊断中的价值

目的　评估 99mTc -HL91肺显像对肺部恶性肿瘤的诊断价值。方法　 17例肺良性病变及 90例肺部恶性肿瘤 (81例原发性肺癌 ,9例肺部转移性瘤 )患者在给药 99mTc -HL91(92 5MBq)后 180min完成前位和后位静态显像。计算病灶和对侧正常肺组织摄取 99mTc -HL91的放射性计数比值 (T/NT)。结果　 99mTc -HL91在肺良性病变的T/NT值为 1.15± 0 .2 5 ;肺恶性病组T/NT为 1.4 5± 0 .2 2 (与良性组比较P <0 .0 0 1,t=4 .998) ,其中鳞癌 1.4 7± 0 .2 7(与良性组比较P <0 .0 0 1,t =4 .137) ,腺癌为 1.4 0± 0 .2 5 (与良性组比较P <0 .0 1,t=3.2 74 ) ,转移性病变为 1.4 6± 0 .2 2 (与良性组比较P <0 .0 1,t =3.12 8)。目测法分析 99mTc -HL91肺显像诊断肺恶性病变的灵敏度为 93.3% (84 / 90 ) ,特异性为 6 4 .7% (11/ 17) ,准确性为 88.8% ;炎性假瘤是最主要的假阳性病变。结论　 99mTc -HL91对诊断及鉴别诊断肺良恶性病变具有明确的价值。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.