类天疱疮与HLA-DRB1基因的相关性研究

为探讨HLA-DRE1基因与类天疱疮（BP）的相关性，采用聚合酶链反应序列特异性寡核苷酸探针PCR-SSOP方法对上海地区汉族56个BP患者和150名健康对照进行了HLA-DRB1基因分型。结果与健康对照组比较，BP患者HLA-DRBI*10（DRB1*1001）基因频率明显增高(Pc=0.022，RR=6.466)。结果提示HLA-DRB1*10(DRB1*1001)可能是我国上海地区汉族BP患者的易感基因。     

山东地区汉族大疱性类天疱疮与HLA-DR,DQB1基因的相关性研究

目的探讨HLA-DR,DQB1位点基因在大疱性类天疱疮(BP)易感性中的作用。方法用序列特异性引物-聚合酶链反应(PCR-SSP)方法,对49例BP患者及70例正常对照者进行了HLA-DR,DQB1等位基因的分型,并分析了上述基因在两组中的分布。结果与正常对照组比较,BP患者组DRB1*10基因频率明显增高(校正P值<0.05);DRB1*04-DQB1*0302连锁体频率、DRB1*10-DQB1*0501连锁体频率在BP组均显著高于对照组;DRB1*04在黏膜损害及大剂量皮质类固醇激素用量组显著增高。结论HLA-DR10(DRB1*10)可能是中国汉族BP的易感基因。DRB1*04-DQB1*0302连锁体、DRB1*10-DQB1*0501连锁体可能为汉族BP的易感连锁体。     

大疱及疱疹性皮肤病

20 0 3 0 91 9　HLA DRB1、DQA1、DQB1基因与上海地区类天疱疮的易感性 /金岩 (复旦大学华山医院皮肤科 )…∥复旦学报 . 2 0 0 3 ,3 0 (1 ) . 2 0～ 2 3采用PCR SSOP方法对上海地区汉族 5 6例BP患者和 1 5 0例健康对照者进行了HLA DRB1、DQA1、DQB1位点等位基因分型。结果发现HLA DRB1 1 0 0 1与DQB1 0 5 0 1紧密连锁 ,其基因频率BP组与对照组比较明显增高 ;DRB1 0 4与DQB1 0 3 0 2紧密连锁 ,其基因频率与对照组比较也明显增高 ;DRB1 1 2基因频率BP组与对照组比较明显降低。因此DRB1 1 0 0 1、DRB1 0 4可…     

内蒙古汉族大疱性类天疱疮与HLA-DRB1和DQB1基因相关性分析

目的探讨内蒙古汉族大疱性类天疱疮与HLA-DRB1和DQB1基因相关性。方法采用聚合酶链反应-序列特异性引物技术(PCR-SSP),检测内蒙古汉族大疱性类天疱疮患者及内蒙古汉族正常人HLADRB1和DQB1基因分型,并统计分析。结果 HLA-DQB1*0301等位基因在大疱性类天疱疮患者组出现频率显著高于对照组(Pc0.05),HLA-DRB1*16和DQB1*0501等位基因在大疱性类天疱疮患者中出现频率显著低于对照组(Pc0.05)。结论 HLA-DQB1*0301可能是内蒙古汉族大疱性类天疱疮患者的遗传易感基因,而HLA-DRB1*16和DQB1*0501可能是内蒙古汉族大疱性类天疱疮患者的保护基因。     

山东地区汉族关节病型银屑病与HLA- DRB1、DQB1的相关性研究

目的 探讨HLA-DRB1、DQB1位点基因与关节病型银屑病的相关性.方法 用序列特异性引物-聚合酶链反应(PCR-SSP)方法,对41例关节病型银屑病患者进行了HLA-DRB1、DQB1等位基因的分型,并分析了上述基因在各组中的分布.结果 关节病型银屑病患者组DRB1*07、DQB1*0201频率较正常对照组增高;多关节炎型银屑病患者组DRB1*07、DQB1*0201以及DRB1*04基因频率比正常对照组显著增高.结论 HLA-DRB1*07、DQB1*0201可能是山东地区汉族关节病型银屑病的遗传标志;具有银屑病易感基因的个体,携带HLA- DRB1*04基因时,患多关节炎型银屑病的危险性可能增加.     

汉族红斑型天疱疮与HLA-Ⅱ类基因的相关性研究

目的 探讨HLA-DR、DQB1位点基因在红斑型天疱疮（PE）易感性中的作用。方法 用聚合酶链反应-序列特异性引物（PCR-SSP）方法，对37例红斑型天疱疮患者进行了HLA-DR、DQB1等位基因的分型，并分别与57例和53例作了对照。结果 与正常对照组比较，PE患者组DR4（DRB1*0406）、DRB1*14、DQB1*0302、DQB1*0503基因频率比对照组显著增高。结论 HLA-DRB1*14、DQB1*0503可能是汉族PE患者易感的单倍型。     

大疱性类天疱疮与HLA-DRB1等位基因的相关性研究

目的:探讨山东汉族大疱性类天疱疮(BP)与HLA-DRB1等位基因的相关性.方法:运用聚合酶链反应-序列特异性引物寡核苷酸探针杂交(PCR-SSOP)方法,对山东地区汉族43例BP患者和125名健康对照组进行了HLA-DRB1等位基因分型.结果:BP患者组HLA-DRB1*10和DRB1*11等位基因的出现频率均高于对照组(P值分别为0.040和0.018),但经校正后P值均无显著性差异;HLA-DRB1*11与BP患者黏膜损害相关(P=0.003,Pc<0.05,RR=12.3).结论:大疱性类天疱疮的遗传易感基因可能与HLA-DRB1等位基因无相关性.     

大疱及疱疹性皮肤病

20062700中国北方汉族寻常型天疱疮与HLA-Ⅱ类基因单倍型的相关性研究/耿龙(中国医大附属第二医院皮肤科),翟宁,韩秀萍…∥中国免疫学杂志.-2006,22(5).-453~455应用序列特异性引物-聚合酶链反应(PCR-SSP)技术对27例中国东北汉族PV患者的HLA-DRB1、DQB1等位基因测定,进行单倍型分析,并与99例健康对照者进行比较。结果显示与对照组比较,寻常型天疱疮患者组中单倍型DRB1*140×-DQB1*0503、DRB1*140×-DQB1*0201、DRB1*120×-DQB1*0503和DRB1*140×-DRB1*0302的频率明显增高,经统计学检验差异有显著意义(P<0.05)。提示特异单…     

北方汉族寻常型银屑病与HLA-DRB1及DQB1等位基因相关性研究

目的:探讨HLA-DRB1及DQB1等位基因与北方汉族寻常型银屑病相关性。方法:利用序列特异性引物-聚合酶链反应(PCR-SSP)分型技术,对63例寻常型银屑病患者和102例健康人的HIA-DRB!及DQB1等位基因进行检测。结果:(1)HLA-DRB1*070x、DRB1*1001及DOB`*020x等位基因与北方汉族寻常型银屑病呈正相关(P分别为0.001,0.005,0.009);HLA-DRB1*120x等位基因与北方汉族寻常型银屑病呈负相关(P=0.007)。(2)HLA-DRB1*070x及DQB1*020x等位基因仅与家族史阳性的早发型(Ⅰ型)银屑病发病相关(P<0.001)。(3)HLA-DRBq*1001等位基因频率在Ⅰ型及无家族史的晚发型(Ⅱ型)银屑病均显著性增高(P<0.05)。结论:(1)HLA-DRB1*070x、DRB1*1001及DQB1*020x等位基因可能是北方汉族寻常型银屑病的易感基因或与易感基因相连锁;HLA-DRB1*120x等位基因可能是阻止北方汉族人发生银屑病的保护基因。(2)Ⅰ型及Ⅱ型银屑病的遗传背景存在差异。     

大疱及疱疹性皮肤病

20 0 32 76 0　 H L A- 类基因与大疱性类天疱疮的相关性研究 /金岩 (复旦大学华山医院皮肤科 )…∥中华皮肤科杂志 .- 2 0 0 3,36 ( 7) .- 36 8～ 371用聚合酶链反应 -序列特异性引物寡核苷酸探针 ( PCR - SSOP )方法对上海地区汉族 56例类天疱疮患者及 150例健康人进行 H LA- DRB1、DQA1、DQ B1等位基因分型。结果显示 ,HL A-DRB1* 10 0 1与 DQ B 1* 0 50 1紧密连锁 ,其基因频率 BP组明显高于对照组 ,与 BP粘膜损害、靶抗原 BP180呈正相关 ;DRB 1* 12基因频率 BP组明显低于对照组 ,与 BP患者年龄和对皮质类固醇的治疗…     

SLE患者皮损部ICAM-1 LFA-1 VCAM-1 ELAM-1的表达

以37例SLE患者为实验对象，应用LSAB试剂盒，用ABC法研究了SLE患者皮损部I－CAM－1、LFA－1、VCAM－1、ELAM－1的表达。结果表皮细胞表面ICAM－1、血管内皮细胞表面ELAM-1、VCAM－1、ICAM－1及浸润细胞表面ICAM－1、LFA－1的表达均增强。说明细胞表面粘附分子与SLE皮损发生有关。     

Subcutaneous administration of collagen-polyvinylpyrrolidone down regulates IL-1beta, TNF-alpha, TGF-beta1, ELAM-1 and VCAM-1 expression in scleroderma skin lesions

In this study the effect of collagen-polyvinylpyrrolidone (collagen-PVP) vs. triamcinolone acetonide (Triam) in scleroderma (SSc) skin lesions was evaluated. Ten SSc patients were treated weekly with subcutaneous injections of 0.2 mL Triam (8 mg/mL) or 0.2 mL collagen-PVP (1.66 mg collagen). Skin biopsies were obtained from lesions before and after treatment. Tissue sections were evaluated by histology and immunohistochemistry (ELAM-1, VCAM-1, IL-1beta, TNF-alpha, TGF-beta1 and PDGF). The corticoid-treated group showed abnormal tissue architecture while the biodrug-treatment restored cutaneous appendages and type I/III collagen proportion. Cytokine and adhesion molecule expression was almost inhibited with Triam, while collagen-PVP down-regulated it. Collagen-PVP improved the tissue architecture of SSc lesions and down-regulated some proinflammatory parameters, without the side effects induced by corticoids.     

Enhanced production of CC-chemokines (RANTES, MCP-1, MIP-1alpha, MIP-1beta, and eotaxin) in patients with atopic dermatitis

Abstract CC-chemokines are potent molecules that direct the migration of leukocytes to inflammatory foci. To determine their role in inflammation associated with atopic dermatitis (AD), we determined serum levels and spontaneous production of CC-chemokines by peripheral blood mononuclear cells (PBMC) in AD patients using an ELISA. Serum levels of RANTES, MCP-1, MIP-1β, and eotaxin were increased in AD patients (n = 52) compared with normal controls (n = 22). Serum levels of RANTES, MCP-1, and MIP-1β were increased in AD patients with severe disease (n = 19) compared with normal controls (n = 22). Spontaneous production of RANTES, MCP-1, MIP-1α and MIP-1β by PBMC was augmented in AD patients (n = 39) and in patients with severe AD (n = 14) compared with normal controls (n = 20). Serum RANTES levels correlated with total serum IgE levels, eosinophil numbers, and serum lactate dehydrogenase levels. Our results suggest that augmented production of CC-chemokines correlates with inflammation associated with AD. Received: 27 June 2000 / Revised: 25 October 2000 / Accepted: 3 March 2001     

Actas Dermo-Sifiliográficas,año 1, número 1

The first issue of Actas Dermo-Sifiliográficas appeared in May-June of 1909. Although not the first Spanish dermatology journal, it did provide a lasting forum where dermatologists could publish in Spanish at the same time as opening a window to the practice of dermatology throughout the world. Initially, the journal only included minutes of the Spanish Society of Dermatology (currently the Spanish Academy of Dermatology and Venereology [AEDV]), certain obituaries, and a section on foreign journals.The first issue of the journal is a good snapshot of the situation of the specialty in Spain 100 years ago. The proportion dedicated to venereology was substantial—more than half the total content. Venereology itself was dominated by syphilis, explaining why the journal retains the word «sifiliográfica» in its title.The catalyst for starting the journal was Juan de Azúa, who was also president of the society, with the help of Sánchez-Covisa as recording secretary, and Miguel Serrano as the society's treasurer and journal manager. The first collaborators were drawn almost entirely from the now defunct Hospital San Juan de Dios in Madrid.     

HLA-DRB1，DQA1，DQB1基因单倍型与华东地区汉族人群白癜风的相关性

目的 探讨HLA-DRB1、DQA1、DQB1基因单倍型与华东地区汉族人群白癜风的相关性。方法 采用聚合酶链反应-序列特异性寡核苷酸探针(PCR-SSOP)方法检测华东地区汉族白癜风患者HLA-DRB1、DQA1、DQB1位点的等位基因,运用遗传学群体与家系资料计算机分析系统3.0筛选并分析单倍型。结果 与正常人对照组比较,HLA-DRB1^*09-DQA1^*03-DQB1^*0303单倍型频率显著增高(Pc=0.02,OR=2.542)。结论 在华东地区汉族人群中,HLA-DRB1^*09-DQA1^*03-DQB1^*0303单倍型可能是白癜风的易感单倍型。     

GSTM1, GSTT1 and GSTP1 gene polymorphism in polymorphous light eruption

Background Polymorphous light eruption (PLE) is the most common chronic and idiopathic photodermatosis. PLE is assumed to represent an immunological hypersensitivity reaction to a radiation‐induced cutaneous antigen involving reactive oxygen species (ROS) on the basis of a genetic predisposition. Among others, cellular protection against ROS is provided by glutathione S‐transferases (GSTs). Different variants of the GST enzymes may influence the activity and efficiency of detoxification and biotransformation of unknown UV‐induced skin‐antigens and other factors that may play an important role in the pathogenesis of PLE. Methods In this study the relationship between isoenzymes of the GST genes GSTM1, GSTT1 and GSTP1 and possible protective or predisposing effects on PLE was examined in 29 patients and 144 controls. Diagnosis of PLE was based on the presence of characteristic clinical features. Results No association between the functional polymorphisms of the GST gene family and PLE was found. Prevalence of certain GST isoenzymes or polymorphisms in patients with PLE did not differ from healthy controls. Conclusion Our data do not support prevalence of GST isoenzymes or polymorphisms as a protective effect against PLE. Especially a higher carrier frequency of GSTP1 Val¹⁰⁵ as a protective factor against PLE which has been published before could not be proved. The GST genotypes GSTM1, GSTT1 and GSTP1 (including SNPs) seem to have no relevant association with PLE.     

Suppressed alloantigen presentation, increased TNF-alpha, IL-1, IL-1Ra, IL-10, and modulation of TNF-R in UV-irradiated human skin

Cytokines induced in skin by ultraviolet radiation cause local and systemic immunosuppression. Tumor necrosis factor alpha, interleukin-1, and interleukin-10 are key mediators in the mouse, but less is known about cytokine synthesis and function in ultraviolet-irradiated human skin. We exposed human skin to 3 minimal erythema doses of solar-simulated radiation and raised suction blisters at intervals to 72 h. Alloantigen presentation was suppressed in a mixed epidermal cell-lymphocyte reaction by 69% from 4 to 15 h post-solar-simulated radiation, but recovered to control values by 24 h. Tumor necrosis factor alpha was raised at 4 h after solar-simulated radiation, reached a maximum 8-fold increase at 15 h, then rapidly declined to control values. Interleukin-1alpha and interleukin-1beta were first increased at 15 h, and remained raised to 72 h, although interleukin-1beta declined from its 15 h maximum. Interleukin-10 increased a maximum 2-fold between 15 and 24 h, coincident with recovery of mixed epidermal cell-lymphocyte reaction responses and downregulation of tumor necrosis factor alpha and interleukin-1beta. Solar-simulated radiation differentially affected soluble tumor necrosis factor alpha receptors; soluble tumor necrosis factor-RI was suppressed 33% at 8-15 h whereas soluble tumor necrosis factor-RII increased 2-fold from 15 to 48 h. Interleukin-1 receptor antagonist was raised at all times post-irradiation. Interleukin-12 was not detectable in control or irradiated skin. These kinetics suggest the tumor necrosis factor alpha network has primary importance in ultraviolet-damaged human skin. The small increase in interleukin-10 implies that 3 minimal erythema doses of solar-simulated radiation is the threshold dose for its induction and local, rather than systemic, functions for interleukin-10 in immunosuppression and regulation of other cytokines.