血竭对大鼠血糖、血浆胰岛素及血脂的影响

目的：观察血竭对大鼠血糖、血浆胰岛素及血脂的影响。方法：用葡萄糖或肾上腺素造成大鼠生理性高血糖模型，用四氧嘧啶造成大鼠糖尿病模型，观察血竭对上述模型动物血糖、血浆胰岛素、血浆总胆固醇、甘油三楷、高密度高脂 蛋白胆固醇的作用。结果：血竭对正常大鼠空腹血糖无明显降低作用，但对葡萄糖及肾上腺素所致高血糖大鼠的血糖水平有降低作用，能改善大鼠对葡萄糖的耐受能力；能降低四氧嘧啶诱导的糖尿病大鼠的空腹血糖水平，增加正常大鼠及糖尿病大鼠的胰岛素分泌；有一定的降血脂作用。结论：血竭对多种原因引起的动物高血糖均有较好的治疗作用和一定的降血脂作用。     

川麦冬多糖降血糖实验研究

目的:考察川麦冬多糖的降血糖作用。方法:用四氧嘧啶诱导的糖尿病小鼠模型,以空腹血糖值为指标研究川麦冬多糖提取物的降血糖作用。结果:川麦冬多糖(400mg/kg)能显著降低四氧嘧啶引起的糖尿病小鼠的空腹血糖和提高血清胰岛素水平。结论:川麦冬多糖具有降血糖作用。     

羊栖菜提取物的降血糖作用研究

目的研究羊栖菜提取物对正常及四氧嘧啶所致糖尿病小鼠血糖的影响。方法用四氧嘧啶糖尿病小鼠为模型,以血糖、糖耐量为指标研究羊栖菜提取物的降血糖作用。结果羊栖菜提取物对正常小鼠无降血糖作用;预防性给予羊栖菜粗多糖(350,700 mg.kg-1)后,四氧嘧啶糖尿病小鼠血糖显著降低;羊栖菜粗多糖和醇提物(700 mg.kg-1)有治疗作用,能明显降低糖尿病小鼠血糖水平;对正常小鼠和糖尿病小鼠糖耐量无明显改善。结论羊栖菜提取物对四氧嘧啶糖尿病小鼠具有防治作用。     

山茱萸不同极性提取物降糖作用的研究

目的:对山茱萸不同极性的提取物进行初步药理学评价,探讨其对正常小鼠、四氧嘧啶糖尿病模型小鼠血糖、血脂及胰岛素的影响。方法:(1)将山茱萸的水提取物、醇提取物、乙酸乙酯提取物、石油醚提取物(浓度皆为1g生药材/mL溶液)以7.5g生药材.kg-1对正常小鼠灌胃,连续7天,观察其降血糖作用。(2)通过灌胃四氧嘧啶(Alloxan)50mg.kg-1×3天分次注射造模建立的小鼠糖尿病模型,以相同剂量的山茱萸不同极性提取物连续7天灌胃,观察其降血糖作用及对胆固醇、甘油三酯和胰岛素水平的影响。结果:山茱萸不同极性提取物对正常小鼠的血糖水平无明显影响。山茱萸醇提取物、乙酸乙酯提取物组与模型组相比,可显著降低四氧嘧啶糖尿病模型小鼠的血糖值(P<0.05),其中山茱萸醇提取物可显著提高血清胰岛素水平(P<0.05);山茱萸乙酸乙酯提取物可显著降低糖尿病模型小鼠血清中甘油三酯含量(P<0.01),山茱萸醇提取物对甘油三酯有降低趋势。结论:山茱萸醇提取物、乙酸乙酯提取物对糖尿病模型动物具有较好的降血糖和降血脂作用。     

灯盏花素对四氧嘧啶致糖尿病小鼠降血糖作用机理探讨

目的:研究灯盏花素对四氧嘧啶致糖尿病小鼠降血糖作用与机理。方法:腹腔注射四氧嘧啶建立糖尿病小鼠模型,分别以高、中、低剂量灯盏花素对糖尿病小鼠尾静脉注射5周,测定小鼠的空腹血糖、葡萄糖耐量、血清中胰岛素水平、胰岛素分泌指数和血清SOD含量,并采用Western-Blot方法检测灯盏花素对糖尿病小鼠胰岛β细胞表达的影响。结果:灯盏花素能显著降低糖尿病小鼠的血糖水平,提高葡萄糖耐量水平(P0.05)并使血清中胰岛素含量升高;提高胰岛素分泌指数;提高血清SOD含量(P0.05);同时提高对四氧嘧啶致糖尿病小鼠胰岛β细胞表达水平(高剂量组P0.01,中剂量组P0.05)。结论:灯盏花素具有减轻四氧嘧啶对胰岛β细胞的损伤程度或对这种损伤具有一定程度的保护和修复作用,能促进胰岛β细胞分泌胰岛素,从而达到降血糖的效果。     

滇黄精对诱导性高血糖小鼠血糖影响的实验研究

目的 观察滇黄精提取物对正常小鼠血糖及几种物质诱导性小鼠高血糖的影响.方法 用10%葡萄糖和240 μg/kg肾上腺素造成小鼠生理性高血糖模型,80 mg/kg四氧嘧啶造成小鼠糖尿病模型,滇黄精提取物与优降糖进行对照,观察滇黄精提取物对上述模型动物血糖的影响.结果 滇黄精提取物对正常小鼠血糖无明显作用;滇黄精提取物能降低葡萄糖性和肾上腺素性高血糖小鼠的血糖水平(P＜0.05);滇黄精提取物能显著降低四氧嘧啶性糖尿病小鼠的血糖水平(P＜0.01).结论 滇黄精提取物对多种原因导致的高血糖小鼠有降糖的作用.     

山绿茶醇提物对四氧嘧啶诱导 糖尿病小鼠的降血糖作用

目的 :研究山绿茶醇提物(IAE)降血糖作用。 方法 :用四氧嘧啶诱导糖尿病小鼠模型,连续ig IAE 12 d,采用葡萄糖GOD-PAP法检测血糖水平,用放射免疫法检测血清胰岛素水平。 结果 :与模型组比较,IAE对四氧嘧啶所致的糖尿病小鼠血糖升高有降低作用(P ＜0.05),高剂量组能升高血清胰岛素水平(P ＜0.01)。 结论 :IAE对四氧嘧啶所致的糖尿病小鼠具有较好的降血糖作用。     

广西匙羹藤茎95%乙醇提取物降血糖作用及其机制的初步研究

目的：研究广西匙羹藤茎95％乙醇提取物（GSEE）的降血糖作用及其机制。方法：采用正常小鼠，四氧嘧啶性糖尿病小鼠，肾上腺素性高血糖小鼠，用放射免疫分析和葡萄糖氧化酶等方法进行研究。结果：GSEE对正常小鼠血糖在较高剂量时有一定影响；可使四氧嘧啶性糖尿病小鼠的血糖值降低、血清胰岛素水平升高；它还可使。肾上腺素性高血糖小鼠血糖降低（P〈0．01）；增强正常小鼠的葡萄糖耐受能力，耐受时间在1h左右。结论：GSEE对四氧嘧啶性糖尿病小鼠及肾上腺素性高血糖小鼠有明显的降血糖作用，并且能增强正常小鼠的葡萄糖耐受。其降血糖的作用机制可能是促进胰岛β细胞分泌胰岛素。     

不同品种桑枝的降血糖实验研究

目的：考察不同品种桑枝的降血糖作用。方法：用四氧嘧啶诱导的糖尿病小鼠模型,以空腹血糖值为指标研究桑枝提取物的降血糖作用。结果：白桑和鲁桑乙醇提取物均能显著降低四氧嘧啶引起的糖尿病小鼠的空腹血糖。结论：白桑和鲁桑具有相似的降血糖作用。     

糖克软胶囊降血糖作用的实验研究

目的:观察糖克软胶囊对糖尿病小鼠的降糖作用并探讨其作用机理。方法:通过静脉注射四氧嘧啶建立糖尿病高血糖小鼠模型,采用One-touch稳捷Ⅱ型血糖仪测血糖及糖耐量,125I-胰岛素放射免疫试剂盒测血清胰岛素水平,并与正常组对照。结果:糖克软胶囊对正常小鼠有显著的降血糖、改善糖耐量作用;可明显改善正常小鼠蔗糖耐量以及四氧嘧啶小鼠葡萄糖耐量;能有效降低四氧嘧啶小鼠高血糖,提高血清胰岛素水平。结论:糖克软胶囊对糖尿病小鼠有较好的降糖作用,其作用机理可能是通过减轻四氧嘧啶对胰岛β细胞损伤,或促进已损伤的β细胞修复,增强胰岛素的分泌功能,从而减轻高血糖反应;而且可通过影响糖的吸收、摄取、降解、代谢利用等降低血糖。     

丹皮多糖降血糖有效成分的筛选及其作用研究

目的：筛选丹皮多糖降血糖的有效成分,研究其降糖作用及机理。方法：用小鼠和大鼠制造葡萄糖性高血糖及四氧嘧啶性糖尿病模型,氢化可的松琥珀酸钠（HCSS）造成胰岛素抵抗,观察丹皮多糖粗品和纯品对正常和高血糖模型动物血糖的影响,并测定糖尿病动物SOD、Insulin、GHb、ApoA1等水平。结果：丹皮多糖纯品2b（PSM2b）可降低葡萄糖和四氧嘧啶诱发的鼠高血糖,优于粗品和其他纯品,并能升高糖尿病小鼠SOD和大鼠ApoA1水平,降低GBb水平,改善小鼠品服葡萄糖耐量和胰岛素抵抗。结论：PSM2b为丹皮多糖中降血糖有效成分,它可有效地控制实验性高血糖,其降糖机理可能与促进外周组织对葡萄糖的作用,提高机体对胰岛素的敏感性有关。     

Alleviating effects of active fraction of Euonymus alatus abundant in flavonoids on diabetic mice

Euonymus alatus (E. alatus) has been used as a folk medicine for diabetes in China for more than one thousand years. In order to identify major active components, effects of different fractions of E. alatus on the plasma glucose levels were investigated in normal mice and alloxan-induced diabetic mice. Our results show that ethyl acetate fraction (EtOAc Fr.) displayed significant effects on reducing plasma glucose. In oral glucose tolerance, EtOAc Fr. at 17.2 mg/kg could significantly decrease the blood glucose of both normal mice and diabetic mice. After 4 weeks administration of the EtOAc Fr, when compared with the diabetic control, there were significant difference in biochemical parameters, such as glycosylated serum protein, superoxide dismutase and malondial dehyde, triglyceride, and total cholesterol, between alloxan-induced diabetic mice and the control group. Additional histopathological studies of pancreatic islets also showed EtOAc Fr. has beneficial effects on diabetic mice. Chemical analysis with three-dimensional HPLC demonstrated that the major components from EtOAc Fr were flavonoids and phenolic acids, which had anti-oxidative effects on scavenging DPPH-radical in vitro. All these experimental results suggest that EtOAc Fr. is an active fraction of E. alatus and can prevent the progress of diabetes. The mechanism of E. alatus for glucose control may be by stimulating insulin release, improving glucose uptake and improving oxidative-stress.     

糖尿乐胶囊降血糖作用的实验研究

目的:观察糖尿乐胶囊的降糖作用。方法:采用正常动物及糖尿病模型动物对糖尿乐的降糖作用进行观察。结果:糖尿乐可降低正常小鼠及大鼠的血糖含量,并可使正常大鼠血清胰岛素水平及肝糖原含量升高;可使肾上腺所致高血糖大鼠血糖明显降低,肝糖原含量明显增加;对四氧嘧啶所致的小鼠和大鼠高血糖均有明显的抑制作用;还能改善大鼠糖耐量。结论:糖尿乐胶囊具有明显的降血糖作用。     

龙血竭小鼠灌胃给药后血中移行成分的分析

目的 对龙血竭进行中药血清药物化学研究,为确定龙血竭的药效成分奠定基础.方法 在建立龙血竭高效液相色谱指纹图谱的基础上,通过比较龙血竭提取物、含药血清和空白血清的HPLC指纹图谱,结合LC-MS/MS检测技术确定龙血竭给药后的血中移行成分.结果 在龙血竭含药血清中发现6个人血成分,其中5个都属原型成分,分别为3,4'-二羟基-5-甲氧基二苯乙烯、剑叶龙血素B、4'-羟基-4,2'-二甲氧基一二氢查耳酮(新化合物)、剑叶龙血素A和龙血素B;1个可能为原型成分或代谢产物.结论 6个人血成分是龙血竭在体内主要直接作用物质,有助于阐明龙血竭的药效物质基础.     

Antihyperglycemic and antilipidperoxidative effects of dry matter of culture broth of Inonotus obliquus in submerged culture on normal and alloxan-diabetes mice

AIM OF THE STUDY: The antihyperglycemic and antilipidperoxidative effects of the dry matter of culture broth (DMCB) of Inonotus obliquus were investigated. MATERIALS AND METHODS: The normal, glucose-induced hyperglycemic and alloxan-induced diabetic mice were used to evaluate the antihyperglycemic and antilipidperoxidative effects of the DMCB of Inonotus obliquus. RESULTS: Treatment with the DMCB (500 and 1000 mg/kg body weight) exhibited a mild hypoglycemic effect in normal mice, and failed to reduce the peak glucose levels after glucose administration. However, euglycemia was achieved in the DMCB of Inonotus obliquus (1000 mg/kg) and glibenclamide-treated mice after 120 min of glucose loading. In alloxan-induced diabetic mice, the DMCB (500 and 1000 mg/kg body weight for 21 days) showed a significant decrease in blood glucose level, the percentages reduction on the 7th day were 11.90 and 15.79%, respectively. However, feeding of this drug for 3 weeks produced reduction was 30.07 and 31.30%. Furthermore, the DMCB treatment significantly decreased serum contents of free fatty acid (FFA), total cholesterol (TC), triglyceride (TG) and low density lipoprotein-cholesterol (LDL-C), whereas effectively increased high density lipoprotein-cholesterol (HDL-C), insulin level and hepatic glycogen contents in liver on diabetic mice. Besides, the DMCB treatment significantly increased catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities except for decreasing maleic dialdehyde (MDA) level in diabetic mice. Histological morphology examination showed that the DMCB restored the damage of pancreas tissues in mice with diabetes mellitus. CONCLUSIONS: The results showed that the DMCB of Inonotus obliquus possesses significant antihyperglycemic, antilipidperoxidative and antioxidant effects in alloxan-induced diabetic mice.     

Hypoglycemic effect of Rehmannia glutinosa oligosaccharide in hyperglycemic and alloxan-induced diabetic rats and its mechanism

The hypoglycemic and anti-diabetic effect of Rehmannia glutinosa oligosaccharide (ROS) in glucose-induced hyperglycemic and alloxan-induced diabetic rats and its mechanism was investigated in this paper. It was found that pretreatment of ROS in normal rats with 100 mg/kg for 3 days, i.p., induced a partial prevention of hyperglycemia caused by glucose (2g/kg, i.p.), while when hyperglycemia was induced in adrenalectomized (ADX) rats, the preventive effect of ROS on hyperglycemia was lost. In alloxan-induced diabetic rats, ROS (100 mg/kg for 15 days, i.p.) showed a significant decrease in blood glucose level and hepatic glucose-6-phosphatase activity with an increase in hepatic glycogen content. Furthermore, ROS raised plasma insulin level and lowered plasma corticosterone level in alloxan-induced diabetic rats. The results indicated that oligosaccharide of Rehmannia glutinosa Libosch. exerted a significant hypoglycemic effect in normal and alloxan-induced diabetic rats. The regulatory mechanism of ROS on glucose metabolism was adrenal dependent and had a close relation with the neuroendocrine system.     

Hypoglycemic effects of Potentilla fulgens L in normal and alloxan-induced diabetic mice

Tap roots of Potentilla fulgens L. traditionally chewed along with betel nut (Areca catechu) and betel leaves (Piper betel), are commonly used by local practitioners for various types of ailments. The crude methanolic extract of the roots was tested for its effects in normoglycemic and alloxan-induced diabetic mice. Hypoglycemic activity was observed to be dose- and time- dependent. The extracts reduced blood glucose level 2 h following administration in both normal and alloxan-induced diabetic mice. In alloxan-induced diabetic mice blood glucose was markedly reduced by 63%, while in normal mice a 31% reduction was observed 24 h after the effective dose of extract was administered. Further, in the diabetic mice a prolonged anti-hyperglycemic action was observed where glucose levels was, found to be significantly low (79%) when compared with control even on the third day. Glucose tolerance was also improved in both normal and diabetic mice. The results were compared against those of insulin, glibenclamide, metformin, and the probable mechanism of action is discussed.