Effects of Different Acupuncture Stimulations on NO Content in Acupoint Areas

Objective：To study the effects of different acupuncture stimulation on nitric oxide（NO） content in acupoint areas.Methods：The experiment was performed in 47 healthy volunteers providing informed consents.Both males and females were included in each group：11 cases in electro-acupuncture group,13 cases in manual-acupuncture group,16 cases in warm-moxibustion group,and 7 cases in control group.Stimulations were given at Jianshi（PC 5） of the Pericardium Meridian and Chengshan（BL 57） of the Bladder Meridian with manual-acupuncture,electro-acupuncture and warm-moxibustion respectively.NO was collected from Ximen（PC 4） and Chengjin（BL 56） areas on the ipsilateral meridians,and the content was detected.Results：The content of NO in the warm-moxibustion group was higher than that in the electro-acupuncture group,the content in electro-acupuncture group was higher than that in the manual acupuncture group（P〈0.05） . Conclusion：All the three stimulations can raise NO levels on acupoint areas significantly,and the effect moxibustion is superior to that of electro-acupuncture and manual acupuncture.     

Clinical Effects of Acupuncture Combined with Nimodipine for Treatment of Vascular Dementia in 30 Cases

To study the therapeutic effects of acupuncture combined with nimodipine for vascular dementia. Methods： Acupuncture was applied at Baihui （GV 20）, Shenshu （BL 23）, Geshu （BL 17）, and the points selected according to the midnight-noon, ebb-flow eight methods of the intelligent turtle, combined with the drug nimodipine. The treatment was continued for 8 consecutive weeks. Results： Of the 30 cases treated, 6 cases were cured, 21 cases improved, and 3 cases failed, with a total effective rate of 90%. Conclusion： Acupuncture at Baihui （GV 20）, Shenshu （BL 23）, Geshu （BL 17）, and the points selected according to the midnight-noon, ebb-flow eight methods of the intelligent turtle combined with the drug nimodipine can yield definite therapeutic effects for vascular dementia.     

针灸治疗幻听症1例

幻听症是出现于听觉器官的虚幻知觉。幻听内容可具有评论性、赞扬性、讥笑性、辱骂性或威胁性,从而使患者高兴或愤怒、激动;也可以具有命令性,使患者有叮能遵照执行而产生拒食、攻击、破坏、自杀等行为。病理性幻听多见于各种精神病以及谵妄、颞叶癫痫、     

面瘫验案1则

1病案实录 郁某,男,18岁。初诊日期：2008年8月18日。患者面瘫已2月余,但失治误治,致症情加重。经亲友介绍来诊。就诊时症见：右眼露睛流泪、不能闭合、白睛红赤,额纹消失,口角向左侧歪斜,流涎,不能鼓嘴吹气,面肌时有抽动;性格内向,情绪抑郁,懒与人交往;纳减,眠少,二便尚调;舌苔薄白,脉小弦数。     

耳针配合灸法治疗功能性子宫出血体会

功能性子宫出血是一种常见的妇科疾病,属中医学“崩漏”范畴,临床主要表现为月经周期不规律、经量过多、经期延长或不规则出血,多由神经系统、内分泌系统功能失调引起。近年来,笔者运用耳针配合灸法治疗本病,取得显著疗效,现将治疗体会简述如下。     

口针治疗不安腿综合征验案1则

不安腿综合征（restless legs syndrome,RLS）是一种至今病因不明、发病机制亦不十分清楚的神经系统感觉运动性疾病,西医治疗本病疗效欠佳,而中医疗法治疗具有一定的优势。笔者运用口针治疗不安腿综合征1例,收效较佳,总结如次,冀为本病的治疗提供一种新的思路。     

Acupuncture Combined with Music Therapy for Treatment of 30 Cases of Cerebral Palsy

To observe clinical therapeutic effects of acupuncture combined with music therapy for treatment of cerebral palsy. Methods： Sixty children with cerebral palsy were randomly divided into an acupuncture group （Group Acup.） and an acupuncture plus music group （Group Acup.＋ M）. Simple acupuncture was applied in Group Acup., and acupuncture at 5 groups of points plus music were applied in Group Acup. ＋M., The treatment was given once every two days with 3 treatments weekly, and 36 treatments constituted a therapeutic course. Therapeutic effects including movement improvement were observed for comparison after 3 courses of treatments. Results： The comprehensive functions were elevated in both groups, and the total effective rate in Group Acup. ＋ M was obviously better than that in Group Acup （P〈0.05）. Movement functions were also improved in both groups, but the differences in improvement of creeping and kneeling, standing, and walking were significant between the two groups （P〈0.01）, showing the effect in Group Acup. ＋ M was better than that in Group Acup.. Conclusion： The therapy of acupuncture plus music gained better therapeutic effect on cerebral palsy than simple acupuncture, which provided new thoughts for treating the disease by comprehensive therapies.     

Thirty-two Cases of Vascular Headache Treated by Acupuncture Combined with Chinese Herbal Decoction

To compare the acupuncture plus oral administration of Chinese herbal decoction with simple oral administration of Chinese herbal decoction in the treatment of vascular headache. Methods： Sixty two patients were randomly divided into a treatment group （32 cases） and a control group （30 cases）. Acupuncture at Baihui （GV 20）, Fengchi （GB 20）, Shuaigu （GB 8）, Xingjian （LR 2）, Neiguan （PC 6）, Sanyinjiao （SP 6） and Ashi points combined with oral administration of Chinese herbal decoction, was applied in the treatment group, and simple oral administration of Chinese herbal decoction was applied in the control group. Results： The total therapeutic effect in the treatment group was better than that in the control group （P〈0.05）. After treatment, the frequency, and duration of the attacks were reduced and shortened, and headache greatly alleviated in both groups （P〈0.01）. The alleviation in the treatment group was more obvious than that in the control group （P〈0.05）. Conclusion： Acupuncture combined with oral administration of Chinese herbal decoction provided remarkable therapeutic effects in treating vascular headache.     

Effect of Acupuncture Combined with Blood-letting by a Three-edged Needle on 50 cases of Bell＇s Palsy at the Acute Stage

Objective： To observe clinical effects of acupuncture combined with blood-letting by a three-edged needle for treating Bell＇s palsy of wind-heat syndrome at the acute stage. Methods： One hundred patients were randomly divided into a treatment group （50 cases） treated by acupuncture plus blood-letting and a control group （50 cases） treated by simple acupuncture. Four courses of treatments were given in both groups. The main symptoms and signs were observed and clinical effects evaluated with scores after one week, 2 weeks and one month of treatments respectively. Results： After treatment, the scores for symptoms and signs were significantly different from those before treatment in both groups （P〈0.05 or P〈0.01）. After one month of treatment, the improvement of the symptoms and signs in the treatment group was more than that in the control group with a significant difference （P〈0.05）. Conclusion： Acupuncture plus blood-letting can produce good therapeutic effects on Bell＇s palsy of wind-heat syndrome at the acute stage.     

A Study on the Classification and the‘Catching’of the‘Arrived Qi’in Acupuncture

This essay studies the phenomenon of‘obtaining of qi’in acupuncture.Combined with clinical practice,the authors think that it was a habitual misunderstanding in the past to equate‘needling sensation’to ‘obtaining of qi’in acupuncture practice.The two concepts are different and are related with each other.The authors have also classified the phenomenon of‘obtaining of qi’according to its depth in the skin layer. Based on the research achievements in neuroscience,the authors propose the study on‘catching’the specific cerebrations,so as to reveal the essence of‘obtaining of qi’.     

Clinical Observation on Acupuncture Treatment of Intractable Insomnia

Objective：To observe the effects of acupuncture for treatment of intractable insomnia.Methods：Totally 90 patients were randomly divided into a treatment group（50 cases） ,treated by the body acupuncture plus auricular-plaster therapy,and a control group（40 cases） ,the Baihui（GV 20） -through-Sishencong（EX-HN1） puncture,auricular-plaster therapy and moxibustion on the back-shu points were not used in the control group.The clinical effects were observed.Results：The total effective rate was 98.0%in the treatment group and 77.5%in the control group.The therapeutic effect in the treatment group was much better than that in the control group（P〈0.01） .Conclusion：The body acupuncture plus auricular-plaster therapy may show better effect for intractable insomnia.     

Depressive Neurosis Treated by Acupuncture for Regulating the Liver -A Report of 176 Cases

Objective： To observe therapeutic effect of acupuncture for regulating the liver on depressive neurosis. Methods： In a multi-center randomized controlled trial, 440 patients were divided into 3 groups： Acupuncture group for regulating the liver （Acup., 176 cases） was treated by acupuncture at Siguan Points, i.e. bilateral Hegu （LI 4） and Taichong （LR 3）, Baihui （GV 20） and Yintang （EX-HN3） plus ear-acupuncture, Prozac group （P., 176 cases） by oral administration of Prozac, and Non-acupoint needling group （NAN, 88 cases） by acupuncture at non-acupoints as acupuncture placebo. Self-rating Depression Scale （SDS） was examined before treatment, and one month, two and three months after treatment respectively to evaluate therapeutic effect, and Rating Scale for Side Effects （SERS） was used to evaluate the safety. Results： After one month of treatment, SDS scores in Acup. Group were significantly lower than that in P. Group （P〈0.05） and than that in NAN Group （P〈0.01）, and SDS scores in P. Group were lower than that in NAN Group （P〈0.05）, showing the SDS scores in Acup. Group 〈P. Group 〈NAN Group. After 2 months of treatment, SDS scores in Acup. Group were also significantly lower than that in P. Group （P〈0.01） and than that in NAN Group （P〈0.01）, and SDS scores in P. Group were also lower than that in NAN Group （P〈0.05）, showing the SDS scores in Acup. Group 〈P. Group 〈NAN Group. After 3 months of treatment, SDS scores in Acup. Group were also significantly lower than that in P. Group （P〈0.01） and than that in NAN Group （P〈0.01）, and SDS scores in P. Group were also lower than that in NAN Group （P〈0.01）, showing the SDS score in Acup. Group 〈P. Group 〈NAN Group. After treatment, SERS scores were 0.16±0.95, 6.51±5.09 and 0.23±1.36 in Acup. Group, P. Group and NAN Group respectively. A significant difference existed between Acup. Group and P. Group （P〈0.05）, but no significant difference between Acup. Group and NAN Group （P〉0.05）, showing the SERS scores in Acup. Group 〈NAN Group 〈P. Group. No side effect was found in Acup. and NAN groups. Conclusion： The therapeutic effect of acupuncture on depressive neurosis is better than or similar to that of Prozac but with less side effect.     

DT-13, a saponin of dwarf lilyturf tuber,exhibits anti-cancer activity by down-regulating C-C chemokine receptor type 5 and vascular endothelial growth factor in MDA-MB-435 cells简

AIM： To investigate the anticancer activity of DT-13 under normoxia and determine the underlying mechanisms of action. METHODS： MDA-MB-435 cell proliferation, migration, and adhesion were performed to assess the anticancer activity of DT-13, a saponin from Ophiopogonjaponicus, in vitro. In addition, the effects of DT-13 on tumor growth and metastasis in vivo were evaluated by orthotopic implantation of MDA-MB-435 cells into nude mice; mRNA levels of vascular endothelial growth factor （VEGF）, C-C chemokine receptor type 5 （CCR5） and hypoxia-inducible factor 1a （HIF-1a） were evaluated by real-time quantitative PCR; and CCR5 protein levels were detected by Western blot assay. RESULTS： At 0.01 to 1 umol·L -1, DT-13 inhibited MDA-MB-435 cell proliferation, migration, and adhesion significantly in vitro. DT-13 reduced VEGF and CCR5 mRNAs, and decreased CCR5 protein expression by down-regulating HIF-1 a. In addition, DT-13 inhibited MDA-MB-435 cell lung metastasis, and restricted tumor growth slightly in vivo. CONCLUSION： DT-13 inhibited MDA-MB-435 cell proliferation, adhesion, and migration in vitro, and lung metastasis in vivo by reducing VEGF, CCR5, and HIF-la expression.     

Qingkailing injection for the treatment of acute stroke： a systematic review and Meta-analysis

OBJECTIVE: To evaluate systematically the clinical efficacy and safety of Qingkailing(QKL) injection in the treatment of acute stroke.METHODS: Searches for randomized controlled trials into acute stroke treated with QKL injection were performed in the China National Knowledge Infrastructure Database, China Science and Technology Journal Database, Wan fang Database,Chinese Biomedical Literature Database, PubMed and Cochrane Library, from January 1979 to March2013. Two reviewers independently retrieved the RCTs and extracted the information. The Cochrane risk of bias method was used to assess the quality of the included studies, and a Meta-analysis was conducted with Review Manager 5.2 software.RESULTS: A total of 13 studies with 1110participants were included. The quality of the studies was generally low. The Meta-analysis indicated that the combined use of QKL and Western Medicine was significantly superior to control group therapy in terms of the total effective rate. The relative risk(RR) in the acute cerebral hemorrhage(ACH) sub-group was 1.17 [95%confidence interval(CI)(1.08, 1.26), P=0.0001]. In the acute cerebral infarction(ACI) sub-group, RR was 1.27 [95% CI(1.14, 1.42), P0.0001], and in the ACH and ACI mixed sub-group, RR was 1.34 [95% CI(1.20,1.50),P0.00001]. Additionally, QKL promoted the absorption of hematoma [mean difference(MD)=﹣3.73, 95%CI(﹣4.48, ﹣2.98), P0.000 01],decreased neurological damage in ACI [MD=﹣5.60, 95% CI(﹣8.50, ﹣2.70), P=0.0002] and ACH[MD= ﹣ 4.08, 95% CI( ﹣ 8.00, ﹣ 0.16), P=0.04],promoted the recovery of awareness [RR=1.56, 95%CI(1.09, 2.21), P=0.01] and reduced the whole blood viscosity coefficient [MD=﹣ 0.75, 95% CI( ﹣1.47, ﹣0.03), P=0.04]. There were no adverse drug reactions reported in the included studies.CONCLUSION: Based on this systematic review,QKL combined with conventional therapy was effective compared with control treatment.However, because the articles used in the study were not of high quality, further studies should be conducted into the efficacy and safety of QKL in treating acute stroke.     

针灸绝骨穴治疗杂病探析

绝骨又名悬钟,属足少阳胆经,为八会穴之髓会。绝骨穴在小腿外侧,外踝上3寸,腓骨前缘。《针灸甲乙经》中记载：“在足外踝上三寸动者脉中,足三阳络,按之阳明脉绝乃取之。”此穴在古今临床应用广泛。《标幽赋》云：“悬钟、环跳,华佗刺蹙足而立行。”     

Hypercoagulability in cancer patients grouped by syndromes differentiated with the theory of abnormal hilit in traditional Uyghur medicine

OBJECTIVE： To investigate the relationship in ma- lignant-neoplasm patients of hypercoagulability between syndromes differentiated with the theory of abnormal hilit in traditional Uyghur medicine（TUM）. METHODS： A total of 248 patients with malignant tumors were enrolled. Based on the theory of TUM they were divided into two groups： abnormal Sav- da and abnormal Non-Savda（including abnormal Khan, abnormal Sepra and abnormal Belghem types）; fifty healthy volunteers were selected as controls. Platelet（PLT）, prothrombin time（PT）, plas- ma fibrinogen（FIB）, thrombin time（TT）, activated partial thromboplastin time（aPTT） and D-Dimer（D-D） were measured in both groups. RESULTS： Compared with the control and abnor-mal Non-Savda groups, in the abnormal Savda group the PLT count increased（P0.05）, the PT was lengthened（P0.01）, and the FIB significantly in- creased（P0.01）. D-Ds in the three groups were sig- nificantly different（P0.05）. No significant differ- ence was found inTT and aPTT values（P0.05）. CONCLUSION： Hypercoagulability existed in pa- tients with malignant tumors in the different types of TUM syndromes, especially in the abnormal Sav- da group; this was characterized by increased blood viscosity, platelet aggregation and thrombo- sis. D-D appears to be a significant predictor for the therapeutic effect of TUM in relation to malignant tumor therapies.     

Effects of feeding tyrosine and phenylalanine on the accumulation of phenylethanoid glycosides to Cistanche deserticola cell suspension culture

AIM： To investigate the effects of feeding phenylalanine （Phe） and tyrosine （Tyr） on the accumulation of total phenolic compounds and four phenylethanoid glycosides （PeGs） to a cell suspension culture of the parasitic plant Cistanche deserticola.METHOD： A cell suspension culture of C. deserticola was established and precursors of different concentrations were fed. In each group, the cell was sampled at the 24^th day after inoculation. The content of total phenolic compounds and four PeGs compounds were determined using the Folin-Ciocalteu method and an HPLC method, respectively.RESULTS： In the Phe fed cells, the maximum PeGs yield was achieved when Phe was fed at 1.5 mmol·L^-1 and the yield reached 1.13 times the control cell concentration. In the Tyr fed cells, the maximum yield of PeGs was 1.60 times of control when 0.75 mmol·L^-1 Tyr was fed to the cells. Furthermore, it was found that the salidroside yield was 4.01 times of control group when 5 mmol·L^-1 Tyr was fed.CONCLUSION： Tyr is a better precursor for PeGs accumulation compared with Phe, and the rate limiting enzymes might be involved in the Tyr branch.     

Capillary blood flow in patients with dysmenorrhea treated with acupuncture

OBJECTIVE; To observe capillary blood flow at acu- points during acupuncture treatment of primary dysmenorrhea and gain new insights into its anal- gesic mechanism. METHODS： Patients with primary dysmenorrhea were enrolled and randomly assigned to a treat- ment or control group. Subjects＇ symptoms were differentiated into various Traditional Chinese Medi- cine （TCM） syndromes and treated for 10 sessions with puncturing acupuncture or self-pressing right-hand Hegu （LI 4）, adding other acupoints based on syndrome. Laser speckle was used tocompare the change in the vasomotor amplitude and perfusion of the capillaries in Hegu （LI 4） be- fore and during the treatment. Each subject was re- quired to finish the period pain symptoms observa- tion form, verbal rating scales, numerical rating scale, pain rating index, face rating scale, Zung self-rating depression scale, Zung self-rating anxi- ety scale, and numerical rating scale before and af- ter treatments. RESULTS： After 10 sessions, the symptom scores, pain index （PI）, and visual analog scale （VAS） de- creased significantly in treatment group. The vol- ume of blood flow in Hegu （LI 4） declined slightly. No significant evidence supported that needling caused capillary contraction, but the capillary vaso- motor amplitude at Hegu （LI 4） increased remark- ably. CONCLUSION： Acupuncture can increase the capil- lary blood flow, thus promoting the flow of Qi and blood in terms of TCM theory, which facilitates pain relief.     

“陆氏针灸思想”辨治病毒性结膜炎1例

<正>"陆氏针灸疗法"在2010年被列入国家级非物质文化遗产。陆氏针灸在针刺方法、配穴方法、切诊方法以及经络学说等方面,均有不少独特的见解。在针刺方法方面,陆氏对"烧山火""透天凉"等针法的实验研究,为临床提供了操作规范。同时,陆氏还进行实验研究,为"导气针法"提供了客观证据。陆氏所提倡的温针、伏针、"五行虚实补泻法"已被许多针灸医生和患者