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1.
Hui Wang Zhenhua Luo Lei Lei Zheng Sun Min Zhou Hongxia Dan Xin Zeng Qianming Chen 《Inflammation》2013,36(3):696-704
The objective of this study was to compare the expression levels of interleukin (IL)-17 IL-17 and IL-23 in serum from patients with both oral lichen planus and chronic periodontitis (OLP-CP), patients only with oral lichen planus (OLP), patients only with chronic periodontitis (CP), and healthy controls (HC). The serum samples were collected from 35 OLP-CP patients, 35 OLP patients, 30 CP patients, and 30 healthy controls. ELISA test was used to detect expression levels of IL-17 and IL-23 in serum from these four groups. ELISA analysis showed significantly elevated levels of serum IL-17 in OLP-CP group compared with OLP group (P?<?0.05) and HC group (P?<?0.01). Serum IL-23 result showed that there was an increased expression level in OLP-CP compared with HC group (P?<?0.01). Additionally, female OLP-CP group showed elevated level of serum IL-17 compared with female OLP group, and also erosive OLP-CP group demonstrated increased serum IL-17 level compared with erosive OLP group. Moreover, analysis showed positive significant correlations of serum IL-17 level with probing depth (P?<?0.05) and plaque index (P?<?0.05) in erosive OLP-CP patients. This study indicates that OLP-CP patients get higher expression level of serum IL-17 and had susceptibility to erosive or female subtype, which indicated that IL-17 may participate in the disease immunopathogenesis of both common oral diseases. 相似文献
2.
Jieh-Neng Wang Jyh-Wei Shin Tsuey-Yu Chang Jiu-Yao Wang Jing-Ming Wu 《Inflammation》2009,32(6):410-418
Intrapleural fibrinolytic therapy (IFT) provides clinical benefit in the treatment of complicated pleural parapneumonic effusion (CPE). Whether IFT influences the proinflammatory cytokines production and fibrinlytic activity is currently unclear. Therefore, we collected pleural effusion samples from CPE patients with IFT (study group) and patients without IFT (control group). A membrane human inflammatory cytokines array kit was used to compare the difference of targeted cytokine production between these two groups. Enzyme-linked immunosorbent assay (ELISA) methods were used for quantitative analysis of targeted cytokines and fibrinolytic enzymes. The results showed there were no significant differences between the study (n = 16) and control (n = 14) groups in patients’ demographic data. After fibrinolytic therapy, the patients in the study group had significant lower plasminogen activator inhibitor (PAI) level (732.36?±?254.09 ng/mL vs 1,509.36?±?1,340.11 ng/mL, p?<?0.05) and higher urokinase plasminogen activator (u-PA) level (75.56?±?41.70 ng/mL vs 6.87?±?5.07 ng/mL, p?<?0.05) than they did before treatment. Moreover, the tissue inhibitors of metalloproteinase-2 (TIMP-2) (1,560.03?±?403.49 pg/mL vs 3,686.45?±?1,263.83 pg/mL, p?<?0.05) and inflammatory chemokine, regulated on activation normal T-cell expressed and secreted/chemokine (C-C motif) ligand 5 (RANTES), (293.58?±?212.93 pg/mL vs 749.27?±?53.79 pg/mL, p?<?0.05), were also significantly lower in the study group after fibrinolytic therapy, but not in the control group. In conclusion, intrapleural fibrinolytic treatment with urokinase could enhance fibrinolytic activity and decrease TIMP-2 and RANTES production. 相似文献
3.
William S. Bradham Michelle J. Ormseth Annette Oeser Joseph F. Solus Tebeb Gebretsadik Ayumi Shintani C. Michael Stein 《Inflammation》2014,37(3):801-808
We examined the hypothesis that insulin resistance (IR) decreases circulating concentrations of N-terminal (NT)-probrain natriuretic peptide (BNP). Obesity, despite being a risk factor for heart failure (HF), is paradoxically associated with lower concentrations of BNP, a marker of myocardial stress. Low BNP in obesity is postulated to be due to IR; however, it has been difficult to define the role of IR independent of obesity. IR in rheumatoid arthritis (RA) is increased, independent of obesity, thus allowing potential mechanistic insights into the relationship between IR and BNP. We measured demographic factors, traditional cardiovascular risk factors, body mass index (BMI), markers of inflammation (interleukin-6 (IL-6), C-reactive protein (CRP), tumor necrosis factor α (TNFα)), NT-proBNP, and IR by the homeostatic model assessment (HOMA) in 140 patients with RA and 82 control subjects. Patients with heart failure and coronary artery disease were excluded. We used multiple linear regression models to examine the relationship between HOMA and NT-proBNP in RA and controls and in RA alone, the additional effect of inflammation. As previously reported, NT-proBNP concentrations were higher in RA (median 80.49 pg/mL, IQR (23.67–167.08 pg/mL)) than controls (17.84 pg/mL (3.28–36.28 pg/mL)) (P?<?0.001), and the prevalence of IR, defined by HOMA?>?2.114, was higher among RA than controls (53 % vs. 15 %, P?>?0.001). HOMA was positively correlated with NT-proBNP (rho?=?0.226, P?=?0.007) in RA, but not in controls (rho?=??0.154, P?=?0.168). In a multivariable model adjusted for age, race, and sex, we found that increasing HOMA was statistically associated with increasing NT-proBNP concentrations in RA (P?=?0.001), but not controls (P?=?0.543) (P for interaction?=?0.036). In RA subjects, when IL-6 was further included in the model, IL-6 (P?=?0.0014), but not HOMA (P?=?0.43), remained significantly associated with NT-proBNP, suggesting that IL-6 may be mechanistically involved in the relationship between IR and NT-proBNP in RA. We conclude that in patients with RA, insulin resistance is associated with higher, rather than the expected lower, concentrations of NT-proBNP and that this may be related to increased IL-6. 相似文献
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5.
目的 通过检测类风湿性关节炎(RA)患者血清中细胞因子白细胞介素(IL)-37、肿瘤坏死因子-α(TNF-α)、IL-18、炎性指标红细胞沉降率以及C-反应蛋白(C-reactionprotein,CRP)水平,观察RA患者临床数据包括压痛关节数,肿胀关节数以及DAS28评分等,探讨RA患者血清IL-37水平升高的意义以其在RA发病机制中可能的作用.方法 80例RA患者、80例健康对照患者,通过酶联免疫吸附试验(ELISA)法检测其血清中细胞因子水平.结果 RA患者血清中IL-37[(40.33±11.25)pg/mL]、TNF-α[(110.41 ±35.37) pg/mL]、IL-18[(121.73±29.22) pg/mL]水平以及红细胞沉降率(ESR)[(42.31±15.02) mm./h]、CRP[(38.31±17.22) mg/L]水平明显高于对照组IL-37[(18.21±5.72) pg/mL]、TNF-α[(30.19±6.82) pg/mL]、IL-18[(55.47±7.29) pg/mL]水平,组间差异有统计学意义(P<0.05).IL-37的表达与TNF-α、IL-18水平呈正相关(相关系数r=0.981,P=0.001).结论 IL-37在RA患者体内高表达并与其它几种炎性因子的表达具有相关性,IL-37可能作为炎性抑制因子参与了RA的发生、发展. 相似文献
6.
Ravi Gupta Anil Kapoor Naveen Mittal Ashwani Soni Sourabh Khatri Gladson David Masih 《The Knee》2018,25(6):1051-1056
Background
The role of an intact meniscus in providing mechanical stability to the knee of anterior cruciate ligament (ACL) deficient and ACL reconstructed patients has not been well studied.Methods
This was a prospective cohort study. A total of 205 patients undergoing ACL reconstruction were enrolled, of which 61 had normal menisci, 49 had a tear in the posterior horn of the medial meniscus (PHMM) (tear < 40% of width?=?19; > 40% of width?=?30), 35 had a tear in the lateral meniscus (< 40% of width?=?15; > 40%?=?20), 13 had a tear in the body and/or anterior horn of the medial meniscus (< 40% of width?=?6; > 40%?=?7) and 47 patients had a tear in both menisci. Patients with a tear in both menisci were excluded. The anterior translation of the tibia (ATT) was calculated preoperatively and postoperatively at three months and six months using KT-1000. Partial meniscectomy was performed in all unstable meniscal tears.Results
The mean age at the time of surgery was 25.2?±?5.1?years. Patients with a normal meniscus showed side to side difference in KT-1000 of 4.8?±?2.5?mm whereas those with a < 40% tear and > 40% tear in PHMM had a difference of 5.36?±?3.07?mm (P?=?0.46) and 7.08?±?2.78?mm (P?=?0.0002), respectively. Patients with a lateral meniscus < 40% and > 40% tear had a mean difference of 5.68?±?2.96?mm (P?=?0.22) and 5.95?±?2.39?mm (P?=?0.09), respectively. Patients with body and/or anterior horn of medial meniscus < 40% and > 40% had a difference of 5.41?±?1.11?mm (P?=?0.59) and 5.78?±?2.38?mm (P?=?0.35), respectively. At three months and six months KT-1000 differences of 2.3?±?1.2?mm and 2.1?±?1.2?mm were seen in patients with normal meniscus; 2.26?±?1.51?mm and 2.16?±?0.9?mm with partial meniscectomy of the PHMM < 40%; 2.65?±?1.53?mm and 2.4?±?1.35?mm with partial meniscectomy of the PHMM > 40%; 2.27?±?1.19?mm and 2.07?±?1.52?mm with partial meniscectomy of the lateral meniscus < 40%; and 2.27?±?1.44?mm and 2.07?±?1.14?mm with partial meniscectomy of the lateral meniscus > 40%; 2.55?±?1.56?mm and 1.91?±?1.09?mm with partial meniscectomy in body and/or anterior horn of medial meniscus < 40% and 2.07?±?1.81 and 2.14?±?1.10?mm with partial meniscectomy in body and/or anterior horn of medial meniscus > 40% (P?>?0.05).Conclusion
PHMM acts as a secondary stabilizer of the knee joint in the absence of functional ACL. There is no effect of partial meniscectomy on mechanical stability of the knee in ACL reconstructed patients. Medial or lateral partial meniscectomy performed at the time of ACL reconstruction does not affect the stability of ACL reconstructed knee. However, the presence of a concomitant tear in PHMM is associated with increased instability in ACL deficient knee. 相似文献7.
Lun Cai Lei Yu Sa Liu Tongxun Li Xiaoping Zhang Wei Cui Jie Du Qinyi Zhang 《Inflammation》2017,40(1):92-99
Accumulating evidence shows that immune cells play an important role in carotid atherosclerotic plaque development. In this study, we assessed the association of 6 different natural killer T (NKT) cell subsets, based on CD57 and CD8 expression, with risk for development of carotid atherosclerotic plaque (CAP). Molecular expression by peripheral NKT cells was evaluated in 13 patients with high-risk CAP and control without carotid stenosis (n?=?18). High-risk CAP patients, compared with healthy subjects, had less percentage of CD57+CD8? NKT cell subsets (8.64?±?10.15 versus 19.62?±?10.8 %; P?=?0.01) and CD57+CD8int NKT cell subsets (4.32?±?3.04 versus 11.87?±?8.56 %; P?=?0.002), with a corresponding increase in the CD57?CD8high NKT cell subsets (33.22?±?11.87 versus 18.66?±?13.68 %; P?=?0.007). Intracellular cytokine staining showed that CD8+ NKT cell subset was the main cytokine-producing NKT cell. Cytokine production in plasma was measured with Bio-Plex assay. The expression levels of pro-inflammatory mediators (IFN-γ, IL-17, IP-10) were significantly higher in CAP patients as compared to that from controls. These data provide evidence that NKT cell subset compartment reconfiguration in patients with carotid stenosis seems to be associated with the occurrence of carotid atherosclerotic plaque and suggest that both pathogenic and protective NKT cell subsets exist. 相似文献
8.
The objective of the present study was to determine the effect of the transportation stress on water buffalo calves. A total of 50 buffalo calves (8?±?1 months old, 165?±?13 kg) were assigned to one of two equal groups; the first group represented clinically healthy non-transported calves (control non-transported group; n?=?25) whereas calves of the second group were subjected to transportation (transported group; n?=?25). Blood samples were collected from control non-transported calves and from transported calves immediately after unloading (post-transportation). The present findings indicated that the examined hematological and biochemical parameters were not significantly (P?≤?0.05) changed in transported calves when compared with the control non-transported group. Furthermore, serum concentration of the investigated acute-phase proteins (APP) namely, haptoglobin (0.37?±?0.01), serum amyloid A (75.43?±?2.11), and fibrinogen (7.51?±?0.25) were significantly (P?≤?0.05) higher in transported calves when compared with control calves (0.1?±?0.01 g/l, 23.9?±?0.56 mg/l, and 4.2?±?0.16 g/l), respectively. Lipid peroxidation represented as malonaldhyde (56.78?±?3.42) was higher significantly (P?≤?0.05), whereas antioxidant biomarkers in the form of nitric oxide (17.68?±?0.89) levels, and activities of superoxide dismutase (7.37?±?0.53) and reduced glutathione (5.25?±?0.95) were lower significantly (P?≤?0.05) in the serum of transported calves when all were compared with the control group (24.68?±?0.19 nmol/g Hb, 21.80?±?0.24 mmol/ml, 9.24?±?0.1 U/g Hb, and 7.23?±?0.21 mmol/l), respectively. Conclusively, the present study demonstrated that transportation were significantly enough to trigger changes in APP and oxidative stress biomarkers in buffalo calves. 相似文献
9.
Diana Castillo-Martínez Maribel Juarez Mariana Patlán Araceli Páez Felipe Massó 《Autoimmunity》2017,50(2):82-85
Aim: To assess serum type III or lambda (λ) interferons (IFN) levels and its clinical and laboratory associations in rheumatoid arthritis (RA). Methods: A cross-sectional study including 43 patients with RA (86% females; age 45.3?±?10.3 years) and 43 healthy individuals was performed. Clinical data including disease activity, acute-phase reactants, rheumatoid factor and anticyclic citrullinated peptide (anti-CCP) antibodies were collected. Serum IFNλ1, IFNλ2, IFNλ3, CXCL8 and anti-mutated citrullinated vimentin (anti-MCV) antibody levels were measured. Results: Patients with RA had higher IFNλ1 (113.5?±?118.6?pg/mL versus 55.9?±?122.3?pg/mL; p?<?0.0001) and IFNλ2 (245.4?±?327.7?pg/mL versus 5.1?±?11.0?pg/mL; p?=?0.009) levels than controls, but not IFNλ3 levels. Notably, IFNλ1 levels were found to be higher in both patients with active disease (124.9?±?135.9?pg/mL; p?<?0.001) and quiescent disease (99.0?±?93.7?pg/mL; p?<?0.01), while IFNλ2 levels were higher only in patients with active disease (264.0?±?356.1?pg/mL; p?=?0.02). A noteworthy association between serum IFNλ1 levels and anti-MCV antibody titers (Spearman's rho coefficient 0.36, 95% CI 0.36 to 0.61; p?=?0.02) was observed. Conclusion: Serum IFNλ1 and IFNλ2 levels are abnormally elevated in patients with RA and the former are linearly associated with circulating anti-MCV antibody levels. These results may place type-III IFN as an attractive new therapeutic target in RA. 相似文献
10.
Jean-Hugues Dalle Adriana Balduzzi Peter Bader Arjan Lankester Isaac Yaniv Jacek Wachowiak Anna Pieczonka Marc Bierings Akif Yesilipek Petr Sedlaçek Marianne Ifversen Sabina Sufliarska Jacek Toporski Evgenia Glogova Ulrike Poetschger Christina Peters 《Biology of blood and marrow transplantation》2018,24(9):1848-1855
Allogeneic hematopoietic stem cell transplantation (HSCT) is beneficial for pediatric patients with relapsed or (very) high-risk acute lymphoblastic leukemia (ALL) in remission. A total of 1115 consecutive patients were included in the ALL SCT 2003 BFM study and the ALL SCT 2007 I-BFM study and were stratified according to relapse risk (standard versus high versus very high risk of relapse) and donor type (matched sibling versus matched donor versus mismatched donor). A total of 148 patients (60% boys; median age, 8.7 years; B cell precursor ALL, 75%) were transplanted from mismatched donors, which was defined as either less than 9/10 HLA-compatible donors or less than 5/6 unrelated cord blood after myeloablative conditioning regimen (total body irradiation based, 67%) for high relapse risk (HRR; n?=?42) or very HRR (VHRR) disease (n?=?106). The stem cell source was either bone marrow (n?=?31), unmanipulated peripheral stem cells (n?=?28), T cell ex vivo depleted peripheral stem cells (n?=?59), or cord blood (n?=?25). The median follow-up was 5.1 years. The 4-year rates of overall survival (OS) and event-free survival were 56%?±?4% and 52%?±?4%, respectively, for the entire cohort. Patients transplanted from mismatched donors for HRR disease obtained remarkable 4-year OS and event-free survival values of 82%?±?6% and 80%?±?6%, respectively, whereas VHRR patients obtained values of 45%?±?5% and 42%?±?5% (P?<?.001), respectively. The cumulative incidence of relapse was 29%?±?4% and that of nonrelapse mortality 19%?±?3%. The cumulative incidence of limited and extensive chronic graft-versus-host disease was 13%?±?3% and 15%?±?4%, respectively, among the 120 patients living beyond day 100. Multivariate analysis showed that OS was lower for transplanted VHRR patients (P?=?.002; hazard ratio [HR], 3.62; 95% confidence interval [CI], 1.60 to 8.20) and for patients beyond second complete remission (CR2) versus first complete remission (P?<?.001; HR, 3.68; 95% CI, 1.79 to 7.56); relapse occurred more frequently in patients with VHRR disease (P?=?.026; HR, 3.30; 95% CI, 1.16 to 9.60) and for those beyond CR2 (P?=?.005; HR, 4.16; 95% CI, 1.52 to 10.59). Nonrelapse mortality was not significantly higher for cytomegalovirus-positive recipients receiving cytomegalovirus-negative grafts (P?=?.12; HR, 1.96; 95% CI, .84 to 4.58). HSCT with a mismatched donor is feasible in pediatric ALL patients but leads to inferior results compared with HSCT with better matched donors, at least for patients transplanted for VHRR disease. The results are strongly affected by disease status. The main cause of treatment failure is still relapse, highlighting the urgent need for interventional strategies after HSCT for patients with residual leukemia before and/or after transplantation 相似文献
11.
Background
The purpose of this study was to characterise the histopathological changes in the infrapatellar fat pad (IPFP) in the early stage of patellofemoral osteoarthritis (PFOA).Methods
Sixty-four New Zealand white rabbits were randomly divided into experimental (n?=?24), sham (n?=?16), and control groups (n?=?24). In the experimental group, denoted as the patellar ligament uneven shortening group (US group), the patellar ligament (PL) was folded eight millimetres and sutured. After eight weeks, all animals were euthanised, and magnetic resonance imaging (MRI) evaluation, wet IPFP weight measurement, and histopathological and immunohistochemistry analysis were performed to analyse the histopathological changes in the IPFPs.Results
The maximum cross-sectional area (CSA) of the IPFPs in the sagittal position of MRI in the control group, sham group, and US group were 45.50?±?7.19?mm2, 45.88?±?6.60?mm2 (vs. control group, P?=?0.907), and 53.83?±?8.24?mm2 (vs. control group, P?=?0.015; vs. sham group, P?=?0.035), respectively. The MRI intensity of the IPFPs in the control group, sham group, and US group were 115.53?±?28.85, 108.53?±?26.73 (vs. control group, P?=?0.589), and 154.52?±?18.48 (vs. control group, P?=?0.002; vs. sham group, P?=?0.002), respectively. The wet weight of the IPFPs in the control group, sham group, and US group were 0.32?±?0.05?g, 0.32?±?0.04?g (vs. control group, P?=?0.895), and 0.38?±?0.06?g (vs. control group, P?=?0.017; vs. sham group, P?=?0.033), respectively. The Osteoarthritis Research Society International (OARSI) scores of the IPFPs in the US group were 6.00?±?1.91, which was higher than the scores of 2.50?±?2.02 (P?<?0.001) in the control group and of 2.75?±?1.67 (P?=?0.001) in the sham group.Conclusions
The histopathological changes of the IPFPs as determined via MRI and microscopic structure appeared to occur much earlier than cartilage damage in PFOA. Furthermore, detecting and treating the IPFP changes may offer aid in the diagnosis and treatment of PFOA. 相似文献12.
Hyoun-Ah Kim Jin-Young Nam Ja-Young Jeon Jeong-Mi An Ju-Yang Jung Chang-Bum Bae Chang-Hee Suh 《Journal of clinical immunology》2013,33(1):143-150
Purpose
Growth arrest-specific protein 6 (Gas6) has been suggested to be a biomarker of disease activity in patients with systemic lupus erthematosus (SLE). We investigated the clinical significance of this protein in Korean SLE.Methods
Blood samples were collected from 150 SLE patients and 50 normal controls (NC). In addition, follow-up samples were collected from 50 SLE patients.Results
Serum Gas6 levels of SLE patients (43.01?±?28.02 ng/mL) were higher than those of NC (20.15?±?9.23 ng/mL, p?<?0.001). When evaluated sensitivity and specificity of the Gas6 for diagnosing SLE using ROC curves, the sensitivity and specificity were 72.7 % and 84 % with a cut-off value of 25.3 ng/mL. In the ROC analysis of Gas6, anti-dsDNA antibody, ESR, complement 3 and complement 4 to identify patients with active lupus, area under the curve (AUC) of Gas6 was highest with 0.763. Serum Gas6 levels were significantly higher in the patients with serositis (70.04?±?30.85 ng/mL) and renal disorder (65.66 ±32.28 ng/mL) compared to those without (41.88?±?27.44 ng/mL, p?=?0.033, 40.3?±?26.33 ng/mL, p?=?0.001, respectively). Gas6 levels were correlated positively with anti-dsDNA antibody (r?=?0.199, p?=?0.015), ESR (r?=?0.204, p?=?0.013) and SLEDAI (r?=?0.512, p?<?0.001). In addition, serum Gas6 levels were correlated negatively with hemoglobin (r?=??0.165, p?=?0.043), lymphocyte count (r?=??0.165, p?=?0.043), complement 3 (r?=??0.343, p?<?0.001) and complement 4 (r?=??0.316, p?<?0.001). Furthermore, change in serum Gas6 levels was correlated with change in SLEDAI levels in the SLE patients that were followed up (r?=?0.524, p?<?0.001).Conclusion
These results suggest that serum Gas6 can be a reliable clinical marker for monitoring disease activity and treatment response in SLE. 相似文献13.
Vahid Zadmajid Mohammad Reza Imanpoor Ali Shabani Akbar Baharlouei 《Comparative clinical pathology》2013,22(4):703-711
The overall objective of the study was to investigate changes in quantitative parameters of goldfish (Carassius auratus) semen, testosterone (T), and gonadosomatic index (GSI) during the four seasons of the year (spring, summer, autumn, and winter). Simple environmental and hormonal treatments were used to induce out-of-season spawning in goldfish. The semen was taken from goldfish in different periods during the four seasons, and the characteristics of sperm and pH were analyzed. Plasma levels of T, GSI, and histological studies of the testes, as well as a range of indices of ovarian development, were measured. No significant differences were observed between volumes of semen which can be extracted per fish, in the four seasons (P?>?0.05). Significant differences were found between sperm motility at different seasons (P?<?0.05), as the maximum total duration of motility was observed in autumn (109.25?±?14.00 s). Sperm density showed a higher value during summer (57.30?±?10.41?spermatozoa (spz)?ml?1) and winter (65.09?±?80.40 spz ml?1) than values that were obtained from spring (48.00?±?7.08 spz ml?1) and autumn (40.42?±?16.54?×?109 spz ml?1) (P?<?0.05). However, spermatocrit (in percent) was higher in winter (39.90?±?4.74) compared with other seasons (P?<?0.05). Values of pH were higher in autumn (7.87?±?0.05) and in winter (7.83?±?0.03) than values that were obtained from other seasons. The peaks of T and GSI during spermiation in spring (T, 21.08 ng/ml, and GSI?=?5.21 %) and in summer (T, 23.32 ng/ml, and GSI?=?6.10 %), when most gonadal development took place, were statistically significantly higher than the levels observed during autumn (T, 15.08 ng/ml, and GSI?=?3.21 %) and winter (T, 22.18 ng/ml, and GSI?=?2.78 %) (P?<?0.05). Our results provided the statistically significant evidence of seasonal variation in sperm characteristics, T and GSI, for goldfish. These findings may be used to: (1) optimize semen collection for hatchery production and (2) characterize the potential impact of seasons on sperm quality and plasma androgen levels. 相似文献
14.
Tewodros Tesfaye Gezahegne Mamo Bojia Endebu Takele Abayneh 《Comparative clinical pathology》2014,23(1):205-212
A cross-sectional study was conducted on the three types of working donkeys in Ethiopia (Abyssinian, Ogaden, and Sennar) while they are in their ecological adaptation sites to evaluate and compare the reference values of serum biochemical profiles. Blood samples were collected from a total of 229 apparently healthy adult working donkeys (134 Abyssinian, 55 Ogaden, 40 Sennar types), and ten serum biochemical analytes (total serum protein, glucose, creatinine, gamma glutamyl transferase (GGT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), sodium, chloride, and potassium) were analyzed using a commercial kit (Centronic GmbH, Wartenberg, Germany) for the target groups. A comparison based on the serum biochemical profile of the three donkey types irrespective of sex has shown significantly lower serum activities of ALT (19.20?±?3.55 IU/l; CI, 18.06–20.34) and AST (178.13?±?55.70; confidence interval (CI), 160.31–195.94) for Sennar donkeys compared to the activities of ALT (23.65?±?7.73; CI, 21.56–25.75) and AST (240.60?±?110.20; CI, 210.81–270.39) (P?<?0.05) for Ogaden-type adult donkeys. A significantly lower serum activity of GGT (36.36?±?14.70 IU/l; CI, 33.85–38.87) was observed for Abyssinian donkeys than for Ogaden donkeys (48.24?±?16.59 IU/l; CI, 43.75–52.72) (P?<?0.001). The serum sodium (147.55?±?21.81 mmol/l; CI, 141.65–153.44) and chloride (116.67?±?16.23 mmol/l; CI, 112.28–121.06) concentrations of Ogaden donkeys were found to be significantly higher than the sodium (132.68?±?14.16 mmol/l; CI, 128.15–137.20) and chloride (104.50?±?6.45 mmol/l; CI, 102.44–106.56) (P?<?0.001) concentrations of Sennar donkeys. A significantly higher serum potassium concentration (4.84?±?0.63 mmol/l; CI, 4.73–4.94) of Abyssinian donkeys was found compared to those of Sennar (4.38?±?0.49 mmol/l; CI, 4.22–4.54) and Ogaden donkeys (4.31?±?0.78 mmol/l; CI, 4.10–4.52) (P?<?0.05). There was no significant variation in the serum concentration of total protein, glucose, creatinine, and ALP activity among the three types of donkeys. A comparison of the serum biochemical profile of adult jacks has shown a significantly different result for the serum activities of GGT, ALT, and AST and the concentrations of serum chloride and sodium among the three donkey types. Abyssinian jennies in Afar had significantly higher serum activities of AST (248.92?±?120.05 IU/l; CI, 202.78–295.07) (P?<?0.05) and ALT (29.04?±?8.34 IU/l; CI, 25.83–32.24) (P?<?0.001) than the AST (192.57?±?66.60 IU/l; CI, 176.97–208.17) and ALT (20.76?±?6.61 IU/l; CI, 19.21–22.31) activities for jennies in Sebeta but had lower serum glucose and chloride concentrations. In conclusion, the findings of the present study could be used as reference values for the serum biochemical parameters of the three types of donkeys in Ethiopia, and clinical interpretation of the biochemical parameter based on the standard reference values should consider the types of donkeys. 相似文献
15.
Although exposure of mosquito larvae to agricultural chemicals such as herbicides is common and widespread, our understanding of how these chemicals affect mosquito ecology and behavior is limited. This study investigated how an environmentally relevant concentration of two herbicides, atrazine and glyphosate, affects mosquito life history traits. One hundred and fifty (150) first instar Aedes (Stegomyia) aegypti (L.) or Aedes (Stegomyia) albopictus (Skuse) larvae were reared in 1.6 L of live oak leaf (Quercus virginiana) infusion in the presence (5 mg/L) or absence (0 mg/L) of atrazine or glyphosate. The containers were monitored daily to determine the emergence rates, sex ratio, male and female emergence times, and female body size. Emergence rates of A. aegypti from atrazine treatment were significantly higher relative to either glyphosate or control treatments (A. aegypti: atrazine?=?93?±?6 % (±95 % CI), glyphosate?=?82?±?5 %, control?=?78?±?5 %), while emergence rates of A. albopictus in atrazine treatments were significantly higher than in glyphosate treatments but not in controls (A. albopictus: atrazine?=?84?±?5 %, glyphosate?=?76?±?4 %, control?=?78?±?4 %). For both mosquito species, a sex ratio distortion with male bias was observed in control and glyphosate treatments, but not in atrazine treatments (A. aegypti: atrazine?=?0.90?±?0.17 (±SE), glyphosate?=?1.63?±?0.21, control?=?1.69?±?0.26; A. albopictus: atrazine?=?1.09?±?0.08, glyphosate?=?1.88?±?0.12, control?=?1.37?±?0.11). Emergence times for both sexes of the two mosquito species were significantly longer in atrazine treatments compared to glyphosate or control treatments (A. aegypti: females: atrazine?=?11.20?±?0.50 (days?±?95 % CI), glyphosate?=?9.71?±?0.23, control?=?9.87?±?0.21; males: atrazine?=?9.46?±?0.27, glyphosate?=?8.80?±?0.25, control?=?8.85?±?0.24; A. albopictus: females: atrazine?=?17.40?±?1.70, glyphosate?=?12.4?±?0.40, control?=?12.5?±?0.30; males: atrazine?=?12.96?±?0.41, glyphosate?=?10.48?±?0.24, control?=?10.64?±?0.37). For A. albopictus but not A. aegypti, adult females from atrazine treatment had significantly longer wing lengths compared to those from glyphosate or control treatments (A. albopictus: atrazine?=?3.06?±?0.07 (mm?±?95 % CI), glyphosate?=?2.80?±?0.07, control?=?2.83?±?0.06). These results demonstrate the potential for atrazine, a widely used herbicide, to influence epidemiologically relevant life history traits of mosquitoes. 相似文献
16.
《Immunobiology》2019,224(4):595-603
In this study, we evaluated serum markers of immune responses in children infected with G. duodenalis and compared them with the characterized parasite isolates. The reactivity indexes (RI) of IgG (1.503 ± 0.819) and IgA (2.308 ± 1.935) antibodies were significantly higher (P < 0.001) in infected children than in non-infected children. There were also statistically significantly higher serum levels (P < 0.05) of IFN-γ (393.10 ± 983.90 pg/mL) as well as serum (30.03 ± 10.92 μmol/L) and saliva nitric oxid derivatives (NOx) (192.4 ± 151.2 μmol/L) in children infected with G. duodenalis compared to the group of non-parasitized children (127.4 ± 274.30 pg/mL; 25.82 ± 7.74 μmol/L and 122.5 ± 105.90 μmol/L, respectively). Regarding the characterized genetic variants of G. duodenalis and the immune response profiles, no differences were observed in terms of antibody reactivity or levels of serum cytokine and NOx among children infected with AI or AII subassemblages. The elevated levels of IFN-γ and NOx indicate that G. duodenalis intestinal infection in humans induces a cellular immune response detectable at the systemic level. Moreover, no significant differences in the antibody reactivity profile or the cytokine and NOx production in the sera of children infected with AI or AII G. duodenalis variants were observed, suggesting that subtypes of the parasite do not influence the immune response profile. 相似文献
17.
Zhengshan Wua b Jianjie Qina b Liyong Pua b aLiver Transplantation Center the First Affiliated Hospital Nanjing Medical University Nanjing Jiangsu China;b 《生物医学研究杂志》2012,26(6):395-399
Omega-3 fatty acid supplemented total parenteral nutrition improves the clinical outcome of patients undergoing certain operations;however,its benefits for patients with hepatitis type B virus(HBV)-associated hepatocellular carcinoma(HCC) who have undergone hepatectomy are still not clear.The aim of this study was to evaluate the effect of omega-3 fatty acid supplemented total parenteral nutrition on the clinical outcome of patients with HBV-associated HCC who underwent hepatectomy at our institution.A total of 63 patients with HBV-associated HCC who underwent hepatectomy were included in this study.These patients were randomly assigned to receive stand-ard total parenteral nutrition(the control group,n = 31) or omega-3 fatty acid supplemented total parenteral nutri-tion(the omega-3 fatty acid group,n = 32) for at least 5 d.The study endpoints were the occurrence of infection-related complications,recovery of liver function and length of hospital stay.The results showed that the omega-3 fatty acid group had a lower infection rate(omega-3 fatty acid,19.4% vs control,43.8%,P < 0.05),a better liver function after hepatectomy:alanine transaminase(omega-3 fatty acid,48.23±18.48 U/L vs control,73.34±40.60 U/L,P < 0.01),aspartate transaminase(omega-3 fatty acid,35.77±14.56 U/L vs control,50.53±24.62 U/L,P < 0.01),total bilirubin(omega-3 fatty acid,24.29±7.40 mmol/L vs control,28.37±8.06 mmol/L,P < 0.05) and a shorter length of hospital stay(omega-3 fatty acid,12.71±2.58 d vs control,15.91±3.23 d,P < 0.01).The serum contents of IL-6(omega-3 fatty acid,23.98±5.63 pg/mL vs control,35.55±7.5 pg/mL,P < 0.01) and TNF-α(ome-ga-3 fatty acid,4.43±1.22 pg/mL vs control,5.96±1.58 pg/mL,P < 0.01) after hepatectomy were significantly lower in the omega-3 fatty acid group than those of the control group.In conclusion,administration of omega-3 fatty acid may reduce infection rate and improve liver function recovery in HBV-associated HCC patients after hepatectomy.This improvement is associated with suppressed production of proinflammatory cytokines in these patients. 相似文献
18.
Youhua Wang Dawei Xu Long Long Xiaolong Deng Ran Tao Guicheng Huang 《Clinical and experimental medicine》2014,14(3):297-304
Osteoarthritis (OA) is a complex disease characterized by cartilage degeneration, secondary synovial membrane inflammation and subchondral bone changes. In recent years, many studies have confirmed that interleukin-18 (IL-18) is involved in the inflammatory process of inflammatory joint diseases. In the present study, we investigated IL-18 levels in plasma, synovial fluid and articular cartilage of patients with primary knee OA (n = 33) to analyze their relationship with radiographic severity. Compared to healthy controls (n = 15), OA patients had higher plasma and synovial fluid IL-18 concentrations (45.8 ± 22.1 vs. 23.7 ± 13.6 pg/ml, P < 0.001 and 75.2 ± 40.1 vs. 28.3 ± 11.6 pg/ml, P < 0.001) as measured by enzyme-linked immunosorbent assay. Also, the percentage of immunofluorescent IL-18 positive cells in articular cartilage was significantly increased in OA compared to controls (46.5 ± 10.3 vs. 2.9 ± 1.7, P < 0.001). Moreover, plasma, synovial fluid and articular cartilage IL-18 significantly positively correlated with radiographic severity, respectively (r = 0.663, P < 0.001, r = 0.56, P = 0.001 and r = 0.884, P < 0.001). Subsequent analysis revealed that plasma, synovial fluid and articular cartilage IL-18 levels positively correlated with each other (r = 0.632, P < 0.001, r = 0.489, P = 0.004 and r = 0.620, P < 0.001). These data suggested that plasma, synovial fluid and articular cartilage IL-18 levels were significantly increased in OA patients, and these elevated levels were positively correlated with radiographic severity. Accordingly, our study supports the role of IL-18 in the pathophysiology of OA. 相似文献
19.
Showkat Ahmad Bhat Bilal Ahmad Bhat Manzoor Ahmad Khan Sabhiya Majid Manzoor R. Mir Athar Ali 《Comparative clinical pathology》2014,23(3):547-549
The study was designed to evaluate the role of antioxidant defense system in the etiology of psoriasis, a chronic skin disorder of complex etiology and pathology. Hospital-based case–control study was carried out in major referral hospital in Kashmir, North India. Cases (N?=?40) were composed of patients with psoriasis vulgaris, and controls (N?=?20) were healthy volunteers. Study included estimation in plasma of both patients and controls of glutathione (GSH) levels, superoxide dismutase (SOD) activity, and total antioxidant potential (AOP) as indices of antioxidant defense system and malondialdehyde (MDA) as a measure of lipid peroxidation (LP), an indicator of oxidative stress. The GSH levels, SOD activity, AOP, and malondialdehyde levels in plasma of psoriasis patients were 2.58?±?0.22 μM/l, 5.24?±?0.69 U/ml, 0.020?±?.011?nmol?1/ml?h, and 0.88?±?0.20 nmol/ml and were 4.76?±?0.52 μM/l, 4.14?±?0.56U/ml, 0.042?±?0.018 nmol?1/ml?h, and 0.53?±?0.16 nmol/ml in healthy voluntary controls, respectively. A significant decrease in GSH levels (p?<?0.005) and AOP (p?<?0.005) and significant increase in SOD activity (p?<?0.01) MDA levels (p?<?0.005) as an indicator of LP was observed. 相似文献
20.
Oral lichen planus (OLP) is generally accepted to be a T cell-mediated chronic inflammatory disease with an unclear pathogenesis. There have been numerous studies on the proliferation and apoptosis of T cells in situ. In contrast, research on the proliferation and apoptosis of peripheral blood mononuclear cells (PBMCs) in patients with OLP is rare. The aim of the present study was to investigate the proliferation and apoptosis of PBMCs in patients with OLP. PBMCs were isolated from 20 patients with reticular OLP, 20 patients with atrophic-erosive OLP, and 20 healthy volunteers. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2,5-tetrazolium bromide assays were performed to investigate the proliferation of PBMCs, and caspase-3 colorimetric assays were performed to investigate the apoptosis of PBMCs. The proliferation rate of PBMCs in atrophic-erosive OLP subjects was significantly higher than that in both healthy (P?<?0.05) and reticular OLP (P?<?0.05) subjects. In contrast, the proliferation rate of PBMCs in reticular OLP subjects was significantly lower than that in healthy subjects (P?<?0.05). The apoptosis rates of PBMCs in OLP subjects (P?<?0.05) and atrophic-erosive OLP subjects (P?<?0.05) were significantly lower than the apoptosis rate in the healthy group. Our findings reinforce the view that T cell-mediated immune responses play a critical role in the pathogenesis of OLP. It can reasonably be concluded that these abnormalities are linked to the presence of inflammatory infiltrates. 相似文献