螺旋藻胶囊

螺旋藻(Spirulina Platensis)是一种呈螺旋状的水生微藻,为单细胞生物、蓝绿色,主要生长于阳光充足、气候炎热的硷性水环境中.国内外科学家研究发现,1kg螺旋藻的营养相当于1000kg各种蔬菜营养的总和,蛋白质含量高达65%,为牛肉的3倍;必需氨基酸含量是普通保健食品的几百倍,还含有大量的多糖、维生素、类胡罗卜素,且细胞壁薄,易于人体消化吸收.因此,联合国工业发展组织向世界各国推荐为“明天最理想的保健食品”.     

新鲜螺旋藻中β-胡萝卜素提取与测定

目的 测定新鲜螺旋藻中β-胡萝卜素的含量,并与螺旋藻藻粉中β-胡萝卜素含量进行比较。方法 通过单因素实验观察超声时间、提取剂体积、提取温度、提取时间对提取β-胡萝卜素含量的影响,利用正交试验确定最佳提取工艺,采用分光光度法对β-胡萝卜素进行含量测定。结果 以石油醚为提取剂,超声20min,提取剂体积15mL,在35℃下浸提10h,测得新鲜螺旋藻中β-胡萝卜素含量为177.95mg/100g,螺旋藻藻粉中β-胡萝卜素含量为117.40mg/100g。结论 新鲜螺旋藻中β-胡萝卜素的含量比螺旋藻粉中的β-胡萝卜素含量高。     

正中隆起内催产素和促性腺激素释放激素相互关系的免疫组化研究

采用免疫组化双染色法研究了催产素(OT)和促性腺激素释放激素(GNRH)阳性神经纤维在大鼠正中隆起(ME)的相互关系。结果表明:OT和GNRH阳性纤维末梢在ME的4个部位相互重叠。①漏斗隐窝(重叠面积为32.32μm~2/1000μm~2);②外带毛细血管周围(17.86μm~2/1000μm~2);③室管膜下层(10.20μm~2/1000μm~2);4.OT纤维与GNRH纤维在行走过程中交互重叠。因此在ME,OT不仅调节GNRH向毛细血管内释放,而且可能调节GNRH在轴突内的运输。     

海南省2018—2021年市售贝类中麻痹性贝类毒素污染状况分析

目的 对海南省2018—2021年市售贝类进行麻痹性贝类毒素（paralytic shellfish poisoning, PSP）检测，以此了解海南省市售贝类中麻痹性贝类毒素污染状况。方法 对2018—2021年海南省13个市（县）常售7种贝类中10种PSP：石房蛤毒素（saxitoxin, STX）、新石房蛤毒素（neosaxitoxin, neoSTX）、漆沟藻毒素1(gonyautoxins 1, GTX1)、漆沟藻毒素2(gonyautoxins 2, GTX2)、漆沟藻毒素3(gonyautoxins 3, GTX3)、漆沟藻毒素4(gonyautoxins 4, GTX4)、漆沟藻毒素5(gonyautoxins 5, GTX5)、脱氧甲酰基类石房蛤毒素（decarbamoylsaxitoxin, dcSTX）、脱氧藤沟藻毒素2(decarbamoylgonyau toxins 2, dcGTX2)、脱氧藤沟藻毒素3(decarbamoylgonyau toxins 3, dcGTX3)的含量进行分析。结果 360份贝类中PSP检出率为10.3%。其中STX检出率最高为5...     

薄层扫描法测定痔克胶囊中硬脂酸的含量

目的　建立痔克胶囊君药壁虎中硬脂酸的含量测定方法。方法　采用双波长薄层扫描法 ,固定相为硅胶G ,流动相为氯仿 甲醇 (90∶10 ) ,检测波长 4 38nm ,参比波长 5 5 0nm条件下进行壁虎中硬脂酸含量测定。结果　硬脂酸的含量在 2 .0～ 7.0 μg范围内线性关系良好 (r =0 .994 4 ,n =6) ,平均回收率为 99.6% (n=5 ) ,RSD为 2 .18% ;本次测定 3批样品 ,每克壁虎中硬脂酸含量平均为 0 .0 836g。结论　本法准确可靠 ,可作为痔克胶囊壁虎中硬脂酸的含量测定指标     

可提高鱼苗存活率的螺旋藻制剂

制备一种提高鱼苗存活率的制剂,原料为螺旋藻Spirulina、颤藻Oscillatoria和蓝藻Cyanophyceae。该制剂可减少幼鱼在养殖期因弧菌、链球菌引起的疾病造成的死亡。如:20000条黄尾狮(yellowtail)幼鱼,平均体重约20g,等分成两组。一为对照组,仅喂饲基本食物(碎沙丁鱼末);另一为研究组,喂饲掺入1％螺旋藻的基本食物,从六月中至八月中,一日二次达两个月。喂食含螺旋藻饲料组在整个养殖期的死亡率均低,累积为25.2％,而对照组     

小儿发热惊厥验方

一、药物组成及制法:荆芥6克,防风6克,钩藤9克,生姜3片,鲜茅根9克。先将上药用水浸泡20分钟(水约600ml)煎煮20分钟,一次煎成,约取汤200～250ml。二、适应症:伤风发热及惊厥。三、用法:初生儿至1岁每次温服10～20ml;2～4岁每服30～50ml;5～7岁每服60～100ml;严重的患儿1小时温服1次,轻则2～4小时温服一次。     

实验性心肺复苏时代谢性酸中毒的矫正;NaHCO_3 Carbicarb和葡萄糖的比较研究

在心跳骤停和心肺复苏(CPR)中,由于细胞内有效氧降低而发生无氧代谢和乳酸性酸中毒(LA)。pH值的降低可抑制心肌功能故需矫正。其机理可能是钙的利用降低或结合型钙蛋白减少或心肌功能下降和对儿茶酚胺类反应性降低。就其自然缓冲力而论,用NaHCO_3可达此目的。但是,用NaHCO_3常效果不佳。据报告NaHCO_3对正常狗可改变冠状动脉血液动力学和代谢,而对人类则可减少心肌利用氧而导致心肌缺血。有人在缺氧性LA模型中使用NaHCO_3,其结果降低心输出量和血压而使血乳酸增加。这是因为NaHCO_3可直接转化为CO_2並迅速进入细胞内引起LA,最近发     

用废弃X线胶片制作投影拷贝

一、X 光胶片处理法将胶片全部浸泡在15～20%工业烧碱或4N～6N 氢氧化钠或4N—6N 硝酸溶液中。20分钟左右(根据 X 光片的曝光的具体情况,浸泡时间可长可短)。待胶片上的药膜层自行脱落时,取出胶片,自来水中洗净,挂起使其自然凉干。即得光滑明亮的拷贝片基。(编者按:一种更经济更简便的方法是:将 X 光胶片放入滚烫的浓石灰水里3～5分钟,即可得到脱药膜的透明胶片。)二、用复印机复制拷贝的方法1.修剪掉片基顶端两侧不平整且有图钉孔眼处,否则输入复印机过程中可造成故障。2.根据需要将片基加工成16～32开大小备用。3.取52～80克/m~2的复印机纸作片基     

健康长寿离不开胆固醇

胆固醇属脂类营养物质,人体内的胆固醇来源于两个途径,即自身合成和膳食中摄入。自身合成的占80%,称内原性胆固醇;膳食摄入的胆固醇占20%,主要来源于动物性食物,称外原性胆固醇。正常情况下成人每天可合成1～1.5克胆固醇,主要在肝脏合成,其次是小肠,肾上腺和脑中含量虽然很高,但合成量甚微。成人每天可从肠道吸收到600～1000毫克胆固醇,当然摄入量多时,吸收量也增加,但每天最多可吸收2克,肠道吸收的胆固醇一半是来自胆汁酸的内原性胆固醇。一、体内胆固醇的分布体内胆固醇含量每千克体重约2克,但各种组织中的含量差别较大。每100克骨骼含10…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.