桂枝挥发油干预LPS致大鼠急性肺炎模型肺Toll样受体2、4和MYD88基因表达的研究

目的:研究桂枝挥发油(VORC)对脂多糖(LPS)致大鼠急性肺炎模型肺Toll样受体2(111R2)、Toll样受体4(TLR4)及其下游信号转导通路髓样分化蛋白88(MyD88))mRNA的影响;方法:VORC(0.1 mL/kg、0.05 mL/kg、0.0025 mL/kg)灌胃(ig)给药5天,末次给药后1 h用LPS(1mg/kg)大鼠尾静脉注射制备急性肺炎模型,注射后6 h无菌取肺,实时荧光定量PCR方法检测,TLR2、TLR4和MYD88 mRNA的表达;结果:LPS尾静脉注射6 h,模型组TLR2、TLR4和MYD88 mRNA较空白组表达相对增高了6.41、6.50、4.89倍;VORC各剂量组TLR2、111R4和MYD88 mRNA均较模型组表达显著降低;结论:桂枝挥发油抗炎的药效学作用可能与抑制TLR2、TLR4和MYD88 mRNA表达,继而抑制其下游信号通路有关.     

电针对急性痛风性关节炎大鼠踝关节滑膜组织TLR/MYD88信号通路的影响

目的 探讨电针对急性痛风性关节炎(acute gouty arthritis,AGA）大鼠踝关节滑膜组织TOLL样受体（TLR）/髓样分化因子88 (MYD88)信号通路相关蛋白表达的影响。方法 将50只SD雄性大鼠随机分为正常组、硅晶体（SMD）组、AGA模型组、西药组、电针组,每组10只;正常组常规喂养,SMD组采用SMD溶液注入关节腔,作为关节炎症模型对照,其余各组采用尿酸钠溶液注射法建立急性痛风性关节炎模型。正常组、SMD组与AGA模型组大鼠造模前2 d开始按20 mL/kg 予以生理盐水灌胃,西药组按1 mg/kg予以秋水仙碱溶液灌胃,电针组选取大鼠受试侧三阴交、解溪、昆仑穴位施针,频率1.5～2 Hz,疏密波,电压9 V,电流强度1～3 mA,留针20 min,上述处理均为1次/d,连续9 d。定期观察各组大鼠受试关节肿胀指数,并采用免疫组化法检测受试关节滑膜组织中TLR2、MYD88蛋白的表达。结果 与正常组比较,SMD组大鼠受试关节肿胀指数增加(P<0.05)、而受试踝关节滑膜组织中TLR2、MYD88蛋白的表达差异无统计学意义（P>0.05）;模型组大鼠受试关节肿胀指数、滑膜组织TLR2、MYD88的表达则明显增加(P<0.05);与模型组比较,西药组和电针组大鼠受试踝关节肿胀指数减小(P<0.05),大鼠受试踝关节滑膜组织中TLR2、MYD88蛋白的表达降低(P<0.05);电针组与西药组比较,差异无统计学意义（P>0.05）。结论 电针能够减轻急性痛风性关节炎的症状,这可能与调节TLR/MYD88信号通路中TLR2、MYD88蛋白的表达有关。     

基于TLR4/MyD88/NF-κB通路异甘草素对大鼠颅脑外伤后炎症反应及Th1/Th2失衡的影响

【摘要】 目的 探讨异甘草素（ISL)通过介导Toll样受体4（TLR4）/髓样分化因子88（MyD88）/核转录因子-κB (NF-κB)通路对大鼠颅脑外伤 （TBI)炎症反应及Th1/Th2细胞失衡的影响。方法将SPF级大鼠随机分为模型组、ISL低剂量组（20 mg·kg-1）、ISL高剂量组（40 mg·kg-1）、阳性药物组（甘油果糖氯化钠,40 mg·kg-1）,每组10只,另设对照组。HE染色观察大鼠脑组织病理学变化;比较血清白细胞介素-1β（IL-1β）、白细胞介素-4（IL-4）、γ干扰素（IFN-γ）、肿瘤坏死因子-α（TNF-α）水平,脑组织TLR4、My D88、NF-κB p65 mRNA及TLR4、My D88、NF-κB p65、磷酸化核转录因子-κB p65（p-NF-κB p65）蛋白表达。结果与对照组比较,模型组血清IL-1β、IL-4、TNF-α水平,脑组织TLR4、My D88 mRNA及TLR4、My D88、p-NF-κB p65蛋白水平升高,血清IFN-γ水平降低（均P＜0.05）;与模型组比较,ISL低剂量组、ISL高剂量组、阳性药物组血清IFN-γ水平升高,IL-1β、IL-4、TNF-α水平降低,脑组织TLR4、My D88 mRNA及TLR4、My D88、p-NF-κB p65蛋白水平降低（均P＜0.05）;与ISL低剂量组比较,ISL高剂量组、阳性药物组血清IFN-γ水平升高,IL-1β、IL-4、TNF-α水平降低,脑组织TLR4、My D88 mRNA及TLR4、My D88、p-NF-κB p65蛋白水平降低（均P＜0.05）;与ISL高剂量组比较,阳性药物组血清IFN-γ水平升高,IL-1β、IL-4、TNF-α水平降低,脑组织TLR4、My D88 mRNA及TLR4、My D88、p-NF-κB p65蛋白水平降低（均P＜0.05）。HE结果显示,ISL低剂量组、ISL高剂量组、阳性药物组脑组织病变较模型组出现不同程度改善,ISL高剂量组和阳性药物组改善尤为明显。结论ISL能有效改善大鼠TBI炎性反应、调节Th1/Th2细胞平衡,可能基于调控TLR4/MyD88/NF-κB通路相关mRNA和蛋白表达发挥作用。     

卡非佐米对大鼠类风湿性关节炎的治疗作用及机制研究

目的探讨卡非佐米（CFZ）对大鼠类风湿性关节炎（RA）的治疗作用及其机制。方法采用随机抽签法将35只大鼠分为预治疗组、同时治疗组、低剂量治疗组（1.0mg/kg）、中剂量治疗组（1.5mg/kg）、高剂量治疗组（2.0mg/kg）、模型对照组和空白对照组7组,每组5只。使用弗氏佐剂诱导大鼠建立药物诱导关节炎（AIA）;在不同时机使用不同剂量CFZ对AIA大鼠进行治疗,观察比较各组间关节炎指数（AI）;免疫组化分析大鼠踝关节诱导型一氧化氮合酶（iNOS）及环氧化酶-2（COX-2）表达水平;抗酒石酸酸性磷酸酶染色评估破骨细胞数;qRT-PCR法检测Toll样受体2（TLR2）、Toll样受体4（TLR4）及髓样分化因子88（MyD88）mRNA表达水平;Westernblot法检测TLR2、TLR4、NF-资B、磷酸化NF-资B（p-NF-资B）及MyD88蛋白表达水平。结果与模型对照组比较,预治疗组、同时治疗组及低、中、高剂量治疗组AI均明显降低（均P＜0.05）,iNOS和COX-2表达水平均下降（均P＜0.05）;中、高剂量治疗组破骨细胞数及积分光密度值均降低（均P＜0.05）;中剂量治疗组和预治疗组MyD88mRNA表达水平均明显下降（均P＜0.05）,高剂量治疗组TLR2、TLR4和MyD88mRNA表达水平均下降（均P＜0.05）;Westernblot结果显示CFZ可明显抑制TLR2、TLR4和NF-资B蛋白表达,在高剂量治疗组中可观察到MyD88蛋白表达抑制。结论CFZ通过下调关节iNOS及COX-2的表达,调控滑膜细胞TLRs/MyD88/NF-资B信号通路,降低关节炎症反应及抑制破骨细胞而起到抗RA效果。     

重组人干扰素对手足口病大鼠的治疗作用及对Toll样受体/髓样分化因子相关信号分子的影响

目的观察重组人干扰素(rh IFN)α-2b对手足口病大鼠的治疗作用及其对Toll样受体(TLR)/髓样分化因子(MyD88)相关信号分子的影响。方法取郑州儿童医院感染性疾病科分离的肠道病毒71-BJ(EV71-BJ),测定EV71-BJ毒株对7日龄无特定病原级Sprauge Dawley(SD)大鼠的半数致死量(LD50)。另取7日龄无特定病原级SD大鼠60只,随机分为对照组、模型组及rh IFNα-2b低、中、高剂量组,每组12只。模型组及rh IFNα-2b低、中、高剂量组大鼠一次性腹腔注射15倍稀释的LD50EV71-BJ毒株100μL,对照组一次性腹腔注射生理盐水100μL,根据各组大鼠的表现进行临床评分,染毒第4天临床评分3～4分者即为建模成功。取rh IFNα-2b低、中、高剂量组建模成功的大鼠,分别给予2、4、8万单位rh IFNα-2b溶入0. 2 m L生理盐水中肌肉注射,对照组、模型组大鼠肌肉注射0. 2 m L生理盐水,每日1次,连续给药3 d。末次干预后24 h脱颈处死各组大鼠,取腹主动脉血,检测各组大鼠全血T淋巴细胞亚群CD4~+、CD8~+、CD4~+/CD8~+及血清γ干扰素(IFN-γ)、白细胞介素(IL)-4、IL-10水平;实时荧光定量聚合酶链反应检测各组大鼠脑组织中TLR2、My D88、核因子-κB(NF-κB)、肿瘤坏死因子相关因子6(TRAF-6) mRNA相对表达量; Western blot法检测各组大鼠脑组织中TLR2、My D88、NF-κB、TRAF-6蛋白相对表达量。结果各组大鼠全血CD4~+、CD8~+、CD4~+/CD8~+、血清IFN-γ、IL-4、IL-10水平及脑组织中TLR2、My D88、NF-κB、TRAF-6 mRNA和蛋白相对表达量比较差异均有统计学意义(F=72.310、57.107、98.928,F=161.175、28.874、22. 637,F=108. 493、122. 121、120. 966、154. 783,F=151. 001、194. 357、89. 442、210. 210,P <0. 05)。与对照组比较,模型组及rh IFNα-2b各剂量组大鼠全血CD4~+、CD4~+/CD8~+及血清IFN-γ水平均显著降低(P <0. 05),全血CD8~+、血清IL-4、IL-10水平及脑组织中TLR2、My D88、NF-κB、TRAF-6 mRNA和蛋白相对表达量均显著升高(P <0.05)。与模型组比较,rh IFNα-2b各剂量组大鼠全血CD4~+、CD4~+/CD8~+及血清IFN-γ水平均显著升高(P <0.05),全血CD8~+、血清IL-4、IL-10水平及脑组织中TLR2、MyD88、NF-κB、TRAF-6 mRNA和蛋白相对表达量均显著降低(P <0. 05)。与rh IFNα-2b低剂量组比较,rh IFNα-2b中、高剂量组大鼠全血CD4~+、CD4~+/CD8~+及血清IFN-γ水平均显著升高(P <0. 05),全血CD8~+、血清IL-4、IL-10水平及脑组织中TLR2、MyD88、NF-κB、TRAF-6 mRNA和蛋白相对表达量均显著降低(P <0. 05)。与rh IFNα-2b中剂量组比较,rh IFNα-2b高剂量组大鼠全血CD4~+、CD4~+/CD8~+及血清IFN-γ水平均显著升高(P <0. 05),全血CD8~+、血清IL-4、IL-10水平及脑组织中TLR2、MyD88、NF-κB、TRAF-6 mRNA和蛋白相对表达量均显著降低(P <0. 05)。结论 rh IFNα-2b可有效改善手足口病大鼠的症状和免疫状态,其中高剂量rh IFNα-2b效果最佳,其作用可能与抑制TLR/MyD88信号通路中相关基因和蛋白表达有关。     

普济消毒饮抑制慢性化脓性中耳炎炎症反应

目的 探讨普济消毒饮抑制慢性化脓性中耳炎炎症反应的作用。方法 采用经鼓膜穿刺途径向鼓室注射铜绿假单胞菌的方法构建慢性化脓性中耳炎小鼠模型。建模成功后，左氧氟沙星组小鼠给予1mg/（kg·d）左氧氟沙星灌胃，普济消毒饮低、中、高剂量组小鼠分别给予2.9g/（kg·d）、5.8g/（kg·d）、11.6g/（kg·d）普济消毒饮浓缩液灌胃，模型组和对照组小鼠均予以等量生理盐水灌胃，每日1次，持续14d。结果 左氧氟沙星和普济消毒饮低、中、高剂量均可减轻中耳黏膜组织病变；与模型组比较，左氧氟沙星组和普济消毒饮低、中、高剂量组小鼠的血清肿瘤坏死因子-α、白细胞介素（interleukin，IL）-6、IL-1β、γ干扰素水平降低（P<0.05），Toll样受体4（Toll-like receptor 4，TLR4）、髓样分化蛋白88（myeloid differentiation protein 88，MYD88）、核因子-κB p65（nuclear factor-κB p65，NF-κB p65）基因表达和蛋白水平及磷酸化NF-κB p65降低（P<0.05），上述指标均呈剂量依赖性。结论 普济消毒饮可抑制慢性化脓性中耳炎炎症反应，可能与抑制TLR4/MYD88/NF-κB信号通路相关。     

急性重症胰腺炎大鼠肝脏Toll样受体4的表达及善宁的干预

目的:探讨Toll样受体4(TLR4)在重症急性胰腺炎(SAP)大鼠肝脏细胞炎症信号转导中的作用和善宁干预作用机制。方法:Wistar大鼠24只,随机分为3组,对照组、模型组、善宁干预组。测定各组血清淀粉酶、TNF-α和IL-6水平,对胰腺组织进行病理学检查和评分,检测肝脏组织标本中TLR4 mRNA表达量和TLR4蛋白活性表达。结果:模型组各项指标均较对照组明显增高(P<0.01);善宁干预组各项指标均较模型组降低(P<0.05),高于对照组(P<0.01)。结论:TLR4影响SAP大鼠肝脏细胞炎症信号转导过程,善宁可能通过影响TLR4的表达,干预SAP大鼠肝脏细胞炎症信号转导过程。     

布地奈德对哮喘模型大鼠肺组织Toll样受体4和5表达的影响

目的观察布地奈德对哮喘模型大鼠肺组织Toll样受体4（TLR4）和TLR5表达的影响,探讨TLR在哮喘炎症机制中的作用。方法 27只SD大鼠随机分成哮喘组、对照组、布地奈德组,每组9只。制备哮喘大鼠模型,免疫组织化学法检测各组大鼠肺组织TLR4和TLR5表达。结果哮喘组大鼠肺组织TLR4光密度值与对照组比较,差异无统计学意义（P〈;0.05）;布地奈德组大鼠肺组织TLR4光密度值显著低于对照组和哮喘组（P〈0.05）。哮喘组大鼠肺组织TLR5光密度值显著高于对照组（P〈0.05）;布地奈德组TLR5与对照组及哮喘组比较,差异均无统计学意义（P〈0.05）。肺组织TLR4和TLR5蛋白表达水平无相关性（r=0.246,P＆gt;0.05）。结论 OVA致敏的哮喘模型大鼠肺组织TLR5表达升高,TLR4无变化,TLR5在哮喘中可能起到促炎作用。布地奈德能下调哮喘模型大鼠肺组织TLR4和TLR5表达。     

吡非尼酮对L-精氨酸诱导的急性胰腺炎小鼠的治疗作用研究

目的通过观察吡非尼酮（PFD）对L-精氨酸诱导的急性胰腺炎（AP）小鼠氧化应激、炎症和细胞凋亡的影响,探讨其对AP的治疗作用。方法32只小鼠随机分为对照组、模型组和PFD低、高剂量组,每组8只,模型组和PFD低、高剂量组小鼠接受2次（间隔1h）腹腔注射4g/kg的20%L-精氨酸溶液（pH=7）诱导AP模型,对照组注射2次等体积0.9%氯化钠溶液（间隔1h）。PFD低和高剂量组小鼠在造模后1、3、6h分别给予200、300mg/kg的PFD溶液灌胃,24h后处死小鼠,收集血样行淀粉酶测定,获得胰腺组织进行病理检查,采用TUNEL法检测凋亡腺泡细胞百分比,采用免疫组化方法检测核因子-资β（NF-资β）表达和髓过氧化物酶（MPO）阳性细胞数,Westernblot法检测4-羟基壬烯醛（4-HNE）的蛋白表达水平。结果模型组血清淀粉酶水平较对照组明显增加（P＜0.05）,而PFD低、高剂量组较模型组均明显减少（均P＜0.05）。模型组小鼠出现严重的胰腺炎症、水肿和脂肪坏死,而PFD低、高剂量组小鼠的组织病理学损伤评分均较模型组明显降低（均P＜0.05）,且PFD低剂量与高剂量组比较差异无统计学意义（P＞0.05）。模型组TUNEL阳性细胞百分比、NF-资β阳性细胞百分比、MPO阳性细胞数、4-HNE蛋白表达水平均较对照组明显增加（均P＜0.05）,而PFD低、高剂量组较模型组上述指标均明显减少（均P＜0.05）。结论PFD可能通过抗氧化、抗炎和抗凋亡等发减轻AP和改善胰腺损伤。     

基于Nrf2/HO-1信号通路探讨迷迭香酸对急性胰腺炎大鼠氧化损伤的影响

目的：基于核因子E2相关因子2(Nrf2)/血红素加氧酶1(HO-1)信号通路探究迷迭香酸（RA）对急性胰腺炎（AP）大鼠氧化损伤的影响。方法：将40只大鼠随机分为Sham组、AP组、RA组、RA+ML385(Nrf2抑制剂)组。采用酶联免疫吸附试验（ELISA）法检测各组大鼠血清淀粉酶（AMY）、脂肪酶（LIP）、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）指标。HE染色检测胰腺组织病理学变化；TUNEL检测胰腺组织细胞凋亡；Western blotting和实时荧光定量PCR(RT-qPCR)法检测胰腺组织中Nrf2、HO-1蛋白和mRNA表达。结果：与Sham组相比，AP组血清中AMY、LIP、IL-1β、IL-6、TNF-α、MDA含量、胰腺指数、胰腺组织病理学评分和细胞凋亡率显著升高，血清中SOD活性、GSH-Px含量、胰腺组织中Nrf2、HO-1 mRNA及蛋白表达量降低（均P<0.05）；与AP组相比，RA组血清中AMY、LIP、IL-1β、...     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.