共查询到20条相似文献，搜索用时 15 毫秒
1.
目的:观察三氧矿合物复合纤维粘连蛋白的盖髓效果, 探讨牙髓细胞分化和修复性牙本质形成的机制, 为临床筛选理想的盖髓剂提供实验依据。方法:将16只猫192颗牙的唇侧开髓, 每只猫的A、B、C、D区选取3颗实验牙, 分别用三氧矿合物、三氧矿合物与100 mg/L纤维粘连蛋白、氢氧化钙、100 mg/L纤维粘连蛋白与医用淀粉盖髓, 运用组织学方法观察修复性牙本质的形成及牙髓组织学改变。结果:术后1周, 4个盖髓组均无修复性牙本质桥的形成;术后4周, 与氢氧化钙、三氧矿合物、纤维粘连蛋白/医用淀粉盖髓比较, 三氧矿合物复合纤维粘连蛋白能形成完整的管样牙本质桥, 牙髓组织炎症反应轻。结论:三氧矿合物复合纤维粘连蛋白盖髓可诱导形成完整的牙本质桥, 牙髓组织反应轻微, 作为生物活性盖髓剂, 起到良好的活髓保存效果, 值得临床推广应用。 相似文献
2.
Ebtesam O. Abo El‐Mal Ashraf M. Abu‐Seida Salma H. El Ashry 《International journal of experimental pathology》2021,102(1):32-44
This study compared the biological effect of Hesperidin, Mineral Trioxide Aggregate (MTA)‐Angelus and calcium hydroxide for direct pulp capping. A total of 126 dogs, teeth were divided according to the post‐treatment evaluation period into three groups (42 teeth each), group I: 2 weeks, group II: 4 weeks and group III: 8 weeks. Each group was further subdivided according to the pulp capping material into three subgroups (14 teeth each), subgroup A (Hesperidin), subgroup B (MTA‐Angelus) and subgroup C (Dycal). Both inflammatory response and dentine bridge formation were assessed by histopathology. All data were statistically analysed. Resolution of the inflammation was recorded by the time with a significant difference between subgroups within the same group (P<.05). Hesperidin, MTA‐Angelus and Dycal showed either mild or moderate inflammation at 2 weeks with significant differences between subgroups (P < .05). At 4 and 8 weeks, there were no significant differences between subgroups (P > .05). Absence of complete or partial calcified bridge with no odontoblastic layer was reported in all subgroups at 2 weeks while at 4 weeks, the majority of samples in Hesperidin and MTA subgroups showed amorphous calcified deposit. At 8 weeks, there was no significant difference (P > .05) between subgroups except that 78.5% and 92.9% of Hesperidin and MTA‐Angelus samples, respectively, showed moderate dentine bridge. Also, 78.5% of Hesperidin and Dycal samples revealed moderately thick dentine bridge while 78.7% of MTA‐Angelus showed a thin dentine bridge with a significant difference between them (P < .05). In conclusion, Hesperidin is a promising pulp capping material inducing mild inflammation and good dentine bridge formation. 相似文献
3.
目的:采用扫描电子显微镜技术观察三氧矿合物复合纤维粘连蛋白作为盖髓剂形成修复性牙本质桥的超微结构特点并对其疗效进行评价。 方法:以4只猫为实验对象,每只猫分为A、B、C、D 4个区,每区选取3颗实验牙,机械暴露牙髓后,分别用三氧矿合物、三氧矿合物复合纤维粘连蛋白、氢氧化钙、纤维粘连蛋白与医用淀粉盖髓,盖髓12周后应用扫描电镜观察新形成的牙本质桥。 结果: 氢氧化钙组、三氧矿合物组可见不规则的牙本质桥结构。三氧矿合物复合纤维粘连蛋白组可见完整的牙本质桥。在纤维粘连蛋白和医用淀粉组,未见牙本质桥形成。结论: 三氧矿合物复合纤维粘连蛋白能刺激修复性牙本质形成,形成的牙本质桥完整,值得临床推广应用。 相似文献
4.
Living dental pulp tissue exposed to the oral environment should be protected with an appropriate pulp capping material to support the dentinogenesis potential of the pulp cells. Mineral trioxide aggregate (MTA) is the material of choice for the treatment of pulp. However, due to cytotoxicity during the initial setting phase of MTA, a new material is required that can act as a barrier to direct contact but facilitate the favorable effect of MTA. This study examined the feasibility of using electrospun poly(ε-caprolactone) fiber (PCL-F) meshes in the MTA-based pulp capping procedures. An experimental pulp capping was performed on the premolars of beagle dogs, and the efficacy of the PCL-F meshes was evaluated after 8 weeks. PCL-F/MTA formed a dentin bridge that was approximately fourfold thicker than that formed by the MTA. Columnar polarized odontoblast-like cells with long processes and tubular dentin-like matrices were observed beneath the dentin bridge in the PCL-F/MTA. The cells were also intensely immunostained for dentin sialoprotein. In cell cultures, PCL-F/MTA reduced cell death to ~8% of that in the MTA group. The proliferation of the cells cultured on PCL-F/MTA was much greater than that of cells cultured on MTA. Furthermore, PCL-F/MTA promoted the differentiation of MDPC23 cells to odontoblast-like cells and biomineralization, as confirmed by the expression of alkaline phosphatase and dentin sialophosphoprotein, and by the deposition of calcium. Based on these histologic findings and the cell responses observed in this study, PCL-F may be used efficiently in the MTA-based dental pulp therapy. 相似文献
5.
Reed Mitchell Jeremy Molligan Sydney Rooney Young Cho Lew Schon Zijun Zhang 《Experimental and molecular pathology》2018,104(1):82-88
Charcot neuroarthropathy (CNA) often presents as a diabetic foot complication. The role of synovial mesenchymal stem cells (syn-MSCs) in the pathogenesis of CNA is unclear. Synovial samples were collected, for isolation of syn-MSCs, from diabetic patients with CNA (n = 7) and non-diabetic patients with intra-articular fracture or normal joints (non-CNA; n = 7) during foot surgery. The syn-MSCs in the CNA and non-CNA groups were characterized comparatively. The average number of colonies formed in the CNA group was 6 ± 3.5 per half plate (10 mm in diameter), while it was 43 ± 21.6 in the non-CNA group (p < 0.05). The average size (pixels) of the colonies in the CNA group was smaller than that in the non-CNA group. When the colonies were stratified into high-, medium- and low-density subgroups, colonies in the high-density subgroup of the CNA group were reduced in density. Expression of PPAR-γ, RUNX2, Sox9 and type II collagen by syn-MSCs in the CNA group was decreased during adipogenic, osteogenic and chondrogenic differentiation as compared with the non-CNA group. In conclusion, syn-MSCs in CNA joints were reduced in number, with declined differentiation potentials. The high-density subpopulation of the syn-MSCs was particularly affected by the pathology of CNA. 相似文献
6.
《Acta biomaterialia》2014,10(6):2804-2813
Antibacterial and remineralizing dental composites and adhesives were recently developed to inhibit biofilm acids and combat secondary caries. It is not clear what effect these materials will have on dental pulps in vivo. The objectives of this study were to investigate the antibacterial and remineralizing restorations in a rat tooth cavity model, and determine pulpal inflammatory response and tertiary dentin formation. Nanoparticles of amorphous calcium phosphate (NACP) and antibacterial dimethylaminododecyl methacrylate (DMADDM) were synthesized and incorporated into a composite and an adhesive. Occlusal cavities were prepared in the first molars of rats and restored with four types of restoration: control composite and adhesive; control plus DMADDM; control plus NACP; and control plus both DMADDM and NACP. At 8 or 30 days, rat molars were harvested for histological analysis. For inflammatory cell response, regardless of time periods, the NACP group and the DMADDM + NACP group showed lower scores (better biocompatibility) than the control group (p = 0.014 for 8 days, p = 0.018 for 30 days). For tissue disorganization, NACP and DMADDM + NACP had better scores than the control (p = 0.027) at 30 days. At 8 days, restorations containing NACP had a tertiary dentin thickness (TDT) that was five- to six-fold that of the control. At 30 days, restorations containing NACP had a TDT that was four- to six-fold that of the control. In conclusion, novel antibacterial and remineralizing restorations were tested in rat teeth in vivo for the first time. Composite and adhesive containing NACP and DMADDM exhibited milder pulpal inflammation and much greater tertiary dentin formation than the control adhesive and composite. Therefore, the novel composite and adhesive containing NACP and DMADDM are promising as a new therapeutic restorative system to not only combat oral pathogens and biofilm acids as shown previously, but also facilitate the healing of the dentin–pulp complex. 相似文献
7.
Chong Hyuk Choi Sung-Jae Kim Yong-Min Chun Sung-Hwan Kim Su-Keon Lee Nam-Kyu Eom Min Jung 《The Knee》2018,25(1):99-108
Background
The purpose of this study was to find appropriate flexion angle and transverse drill angle for optimal femoral tunnels of anteromedial (AM) bundle and posterolateral (PL) bundle in double-bundle ACL reconstruction using transportal technique.Methods
Thirty three-dimensional knee models were reconstructed. Knee flexion angles were altered from 100° to 130° at intervals of 10°. Maximum transverse drill angle (MTA), MTA minus 10° and 20° were set up. Twelve different tunnels were determined by four flexion angles and three transverse drill angles for each bundle. Tunnel length, wall breakage, inter-tunnel communication and graft-bending angle were assessed.Results
Mean tunnel length of AM bundle was > 30 mm at 120° and 130° of flexion in all transverse drill angles. Mean tunnel length of PL bundle was > 30 mm during every condition. There were ≥ 1 cases of wall breakage except at 120° and 130° of flexion with MTA for AM bundle. There was no case of wall breakage for PL bundle. Considering inter-tunnel gap of >2 mm without communication and obtuse graft-bending angle, 120° of flexion and MTA could be recommended as optimal condition for femoral tunnels of AM and PL bundles.Conclusion
Flexion angle and transverse drill angle had combined effect on femoral tunnel in double-bundle ACL reconstruction using transportal technique. Achieving flexion angle of 120° and transverse drill angle close to the medial femoral condyle could be recommended as optimal condition for femoral tunnels of AM and PL bundles to avoid insufficient tunnel length, wall breakage, inter-tunnel communication and acute graft-bending angle. 相似文献8.
Gordana Dragović Božana Dimitrijević Al Musalhi Khawla Ivan Soldatović Mladen Andjić Djordje Jevtović Devaki Nair 《Experimental and molecular pathology》2017,102(2):210-214
Background
The role of interleukins in the pathogenesis of lipodystrophy in HIV/AIDS-patients is still not understood. The aim of this study was to evaluate the relationship between serum levels of interleukins between HIV/AIDS-patients with or without lipodystrophy, as well as between different subgroups of lipodystrophy (lipoatrophy, lipohypertrophy, mixed-fat-redistribution) and patients without lipodystrophy.Methods
Cross-sectional study of 66 HIV/AIDS patients, all Caucasians. Serum levels of interleukins (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10) were measured using Cytokine-Array-1 on Evidence Investigator, Biochip Array Technology. The associations between interleukins and anthropometric and metabolic variables were estimated by Spearman-correlation. Analysis of covariance with bootstrapping method (ACBM) was used to examine relationship between interleukins and lipodystrophy categories adjusted for confounding variables.Results
The lipodystrophy was observed in 29 (44%) patients, while 15 (52%) had lipoatrophy, 4 (14%) lipohypertrophy and 10 (34%) patients had mixed fat redistribution. There were 37 (56%) patients without lipodystrophy. Significantly lower levels of IL-4 and IL-10 were observed in lipodystrophy vs. non-lipodystrophy (p = 0.008; p = 0.027, respectively). No differences were found relating IL-1α, IL-1β, IL-2, IL-6 and IL-8 levels in lipodystrophy vs. non-lipodystrophy. In patient subgroup with lipoatrophy, significantly lower levels of IL-4 and IL-10 were found when compared to non-lipodystrophy (p = 0.043; p = 0.031, respectively). In lipohypertrophy subgroup significantly lower levels of IL-4 were found when compared to non-lipodystrophy (p = 0.003). In order to estimate the correlation of IL-4 and IL-10 and the presence of lipodystrophy, ACBM showed that correlation of IL-4 levels in patients with lipodystrophy remains statistically significant (p = 0.004) in all types of lipodystrophy: lipoatrophy, lipohypertrophy and mix-fat-redistribution (p = 0.027; p = 0.009; p = 0.017, respectively) after adjustment for age, BMI.Conclusions
IL-4 and IL-10 levels were significantly lower in lipodystrophy vs. non-lipodystrophy. According to our knowledge, we showed for the first time significant correlation between IL-4 levels and lipodystrophy development in HIV/AIDS patients. 相似文献9.
Hubert de Boysson Aurélie Daumas Mathieu Vautier Jean-Jacques Parienti Eric Liozon Marc Lambert Maxime Samson Mikael Ebbo Anael Dumont Audrey Sultan Bernard Bonnotte Alain Manrique Boris Bienvenu David Saadoun Achille Aouba 《Autoimmunity reviews》2018,17(4):391-398
Objectives
Large-vessel involvement (LVI) can occur in giant-cell arteritis (GCA) and may represent a distinct disease subgroup with a higher risk for aortic dilation. This study aimed to better characterize the presentation and evolution of LVI in patients with GCA.Patients and methods
A retrospective multicenter study enrolled 248 GCA patients with LVI and 301 GCA patients without LVI on imaging. Factors associated with aortic dilation were identified in a multivariable model.Results
The patients with LVI were younger (p < 0.0001), more likely to be women (p = 0.01), and showed fewer cephalic symptoms (p < 0.0001) and polymyalgia rheumatica (p = 0.001) but more extracranial vascular symptoms (p = 0.05) than the patients without LVI. Glucocorticoids (GC) management did not differ between the two groups, but the GC discontinuation rate was lower in the patients with LVI (p = 0.0003). Repeated aortic imaging procedures were performed at 19 months [range: 5–162 months] and 17 months [range: 6–168 months] after diagnosis in 154 patients with LVI and 123 patients without LVI, respectively, of whom 21% and 7%, respectively, presented new aortic dilations (p = 0.0008). In the patients with LVI, aortic dilation occurred on an aorta segment shown to be inflammatory on previous imaging in 94% of patients. In the multivariate analysis, LVI was the strongest predictor of aortic dilation (hazard ratio: 3.16 [range: 1.34–7.48], p = 0.009).Conclusions
LVI represents a distinct disease pattern of GCA with an increased risk of aortic dilation. Control of the aortic morphology during follow-up is required. 相似文献10.
Background
In total knee arthroplasty (TKA), femoral component rotation is an important factor in the flexion stability and biomechanics of the patellofemoral joint. However, it remains unclear how much tension is appropriate when performing TKA using the gap technique.Methods
One hundred fifty TKAs that used the gap technique were randomized into one of the three groups. Gravity group (n = 50) included patients who underwent TKA using only the weight of the patient's lower leg. In 20-lbf group (n = 50) and 30-lbf group (n = 50), a gap-tensioning device was set at 20 lbf and 30 lbf respectively. The femoral component rotation was measured based on the clinical transepicondylar axis (cTEA) on postoperative CT and any outliers (a femoral component rotation diverging from the cTEA over 3°) were evaluated.Results
The mean femoral component rotation was ? 0.82° ± 2.44° (95% confidence interval [CI], ? 1.52° to ? 0.13°) in gravity group, ? 0.40° ± 2.22° (95% CI, ? 1.03° to 0.23°) in 20-lbf group, and 1.37° ± 2.70° (95% CI, 0.61° to 2.14°) in 30-lbf group. The mean femoral component rotation in 30-lbf group was significantly different from that in gravity group (p < 0.001) and 20-lbf group (p < 0.001). There were more outliers in 30-lbf group (18% in gravity group, 18% in 20-lbf group, and 36% in 30-lbf group; p = 0.043).Conclusions
The use of a tensioning device set at 30 lbf resulted in an externally rotated femoral component and frequent outliers. 相似文献11.
Seung-Suk Seo Chang-Wan Kim Chang-Rack Lee Jin-Hyuk Seo Do-Hun Kim Ok-Gul Kim 《The Knee》2018,25(1):167-176
Background
This study aimed to assess the incidence of genu recurvatum without neuromuscular disorders in knees that underwent navigation-assisted total knee arthroplasty (TKA), to evaluate short-term radiologic and clinical results of navigation-assisted TKA in genu recurvatum, and to evaluate differences in results according to the degree of pre-operative hyperextension and type of implant and insert.Methods
This study retrospectively reviewed 510 knees that underwent navigation-assisted TKA from January 2005 to December 2011. The incidence of knees that showed hyperextension of ≥ 5° (genu recurvatum) on navigation, and the accompanying alignment were evaluated. It assessed radiologic, intraoperative, and clinical results in recurvatum and control groups by using propensity score matching.Results
A total of 465 knees underwent navigation-assisted TKA for degenerative osteoarthritis. Genu recurvatum was observed in 55 knees (11.8%). Of these, 41 knees (74.5%) had degree of hyperextension between five degrees and 10°, and 47 (85.4%) had varus alignment. The thickness of the resected distal femur in the recurvatum group (7.6 ± 1.6 mm) was less than that in the control group (8.4 ± 1.4 mm, P = 0.001). The thickness of the insert in the recurvatum group (12.5 ± 2.3 mm) was greater than in the control group (10.8 ± 1.5 mm, P < 0.001). The sagittal alignment at the final follow-up was 1.3 ± 3.4° in the control group and ? 0.1 ± 0.7° in the recurvatum group (P = 0.003). Subgroup analyses in the recurvatum group showed no significant difference in sagittal alignment and patient-related outcomes by degree of pre-operative hyperextension and implant/insert type (P > 0.05 for all parameters).Conclusions
Genu recurvatum was not uncommon among patients undergoing primary TKA. This review obtained satisfactory short-term clinical and radiologic results, with a smaller distal femoral resection and thicker insert. 相似文献12.
João Lucas O’Connell Minna Moreira Dias Romano Erica C. Campos Pulici Eduardo E.V. Carvalho Fernanda R. de Souza Denise M. Tanaka Benedito Carlos Maciel Hélio C. Salgado Rubens Fazan-Júnior Marcos A. Rossi Marcus V. Simões 《Experimental and toxicologic pathology》2017,69(4):213-219
Objectives
Doxorubicin (DXR), an anthracyclic antineoplastic agent, is one of the most commonly drug utilized to induce dilated cardiomyopathy (DCM) and heart failure (HF), but the well optimized protocol for cardiomyopathy induction leading to development of cardiac systolic dysfunction is unclear. This study aims to critically compare short-term and long-term DXR injection protocols for the induction of DCM in rats.Methods
Animals were allocated into 3 experimental groups: a ST (short-term DXR injection) group, in which animals received 6 intraperitoneal (i.p.) injections of DXR (2.5 mg/kg per dose) over a period of 2 weeks (cumulative dose of 15 mg/kg); a LT (long-term DXR injection) group in which animals received weekly i.p. injections of DXR (2 mg/kg per dose) over a period of 9 weeks (cumulative dose of 18 mg/kg); and a control group in which animals received an appropriate volume of 0.9% saline i.p. All animals were submitted to echocardiography analysis at baseline and after completion treatment. Afterwards, the hearts were collected for conventional light microscopy and collagen quantification.Results
Morphological myocardial analysis of both DXR-treated groups showed an identical pattern of swollen and vacuolated cardiomyocytes and disorganization of myofibrils. There was pronounced interstitial fibrosis in both groups of DXR-treated hearts as compared to controls, as assessed by the interstitial collagen volume fraction. There was no difference in interstitial fibrosis between the ST and LT groups. The echocardiography analysis of the LT group showed structural and functional findings compatible with DCM, including increased left ventricular systolic (5.02 ± 0.96 mm) and diastolic (7.68 ± 0.96 mm) dimensions and reduction of ejection fraction (69.40 ± 8.51%) as compared to the ST group (4.10 ± 0.89 mm, 7.32 ± 0.84, and 79.68 ± 7.23%, respectively) and control group (4.07 ± 0.72 mm, 7.17 ± 0.68 mm and 80.08 ± 4.71%, respectively), ANOVA p < 0.01.Conclusions
These results indicate that LT injection of DXR is more effective than ST injection in inducing left ventricular dysfunction and structural cardiac changes resembling those found in dilated cardiomyopathy. 相似文献13.
L. Essola L. Kouégnigan Rérambiah R. Obame H. Issembè A. Sima Zué 《Transfusion Clinique et Biologique》2017,24(2):52-55
Objectives
To evaluate if the association of injectable iron and tranexamic acid allows a significant saving in transfusion, in cases of myomectomies and hysterectomies.Patients and method
This is a prospective, non randomized study done over 8 months (from January 2013 to August 2013). Were included, patients undergoing hysterectomy or myomectomy who had a hemoglobin level greater than or equal to 8 g/dl and less than 12 g/dl. Two groups were compared: group A consisting of patients for whom a pack red cells was ordered and the group B which patients received intravenous iron preoperatively and tranexamic acid perioperatively. The level of hemoglobin, pre- and postoperative, the average number of blood units per patient and estimated blood loss was compared. The transfusion economy was evaluated.Results
During this period, 87 patients with a mean age of 40 ± 9 years (range: 23 and 70 years) were included according to our criteria: 44 patients in group A and 43 patients in group B. Initial mean hemoglobin in both groups was 9.1 ± 0.7 g/dl. In group B, after iron administration, the mean hemoglobin was 11.3 ± 0.7 g/dl. The average number of red blood cells received intraoperative patient in group A was 1.54 ± 0.51. The estimated blood loss was significant greater (P = 0.0002) in group A (571.6 ± 237.1 ml) than in group B (213.7 ± 131.7 ml). No transfusion was performed in group B.Conclusion
The association intravenous iron and tranexamic acid resulted in the reduction of transfusion requirements in our setting. It could be integrated in the strategy for sparing blood transfusion in scheduled surgery with hemorrhagic risks. 相似文献14.
Shahab-Aldin Akbarian Amin Salehi-Abargouei Makan Pourmasoumi Roya Kelishadi Parvaneh Nikpour Motahar Heidari-Beni 《Advances in medical sciences》2018,63(1):43-56
Background
Many studies with inconsistent results have assessed the association of Brain-derived neurotrophic factor (BDNF) gene polymorphisms with prevalence of obesity and overweight. This review aims to provide a summary of the literature evaluating the relation between BDNF genotype and body mass index (BMI).Methods
A systematic search through PubMed, Scopus, Science direct, Ovid and Cochrane was performed. We included observational studies with cross-sectional and case-control design, which investigated relationship between all kinds of BDNF polymorphisms with BMI, as a representative index of obesity and overweight. Newcastle–Ottawa Scale was used to assess the quality of included articles.Results
Thirty five studies were included in quantitative synthesis. Analyses were performed separately using OR, β coefficient and mean. Significant association were documented between rs925946 and BMI (OR = 1.12, 95% CI = 1.08–1.17, P heterogeneity = 0.317), rs10501087 and BMI (OR = 1.14, 95% CI = 1.04–1.24, P heterogeneity = 0.861), rs6265 and BMI (OR = 1.13, 95% CI = 1.07–1.19, P heterogeneity = 0.406), rs988712 and BMI (OR = 1.29, 95% CI = 1.18–1.40, P heterogeneity = 0.602). According to pooled β coefficient analysis, significant result was only observed in the rs925946 polymorphism subgroup. Pooled mean analysis showed that overall effects for the association between BDNF polymorphisms and BMI were not statistically significant.Conclusion
This meta-analysis suggests that some polymorphisms in BDNF gene including rs925946, rs10501087, rs6265 and rs988712 can be considered as genetic determinants of obesity. 相似文献15.
Won-Young Park So-Jeong Lee Young-Keum Kim Ahrong Kim Do Youn Park Bong-Eun Lee Geun-Am Song Gwang Ha Kim 《Pathology, research and practice》2018,214(1):95-99
Aims
Endoscopic resection is a safe and effective method to treat gastric epithelia dysplasia (GED). However, the development of metachronous and synchronous lesions after treatment has become a major concern. In this study, we investigated clinicopathologic features of 105 GED lesions from endoscopic resections between January 2008 and December 2009. Our goal is to find histologic factors that predict synchronous and metachronous lesions after ESD treatment. We assessed the degree of intestinal metaplasia (IM) and atrophy, type of IM, presence of gastritis cystica profunda, and crypt dysplasia in the adjacent mucosa.Methods and results
We divided 105 GED lesions into three groups: a single group without metachronous or synchronous GED or adenocarcinoma (n = 35); a multiple synchronous group (n = 30, group with synchronous occurrence of GED or adenocarcinoma after treatment); and a multiple metachronous group (n = 40, group with metachronous occurrence of GED or adenocarcinoma after treatment). The multiple metachronous and synchronous groups showed larger sizes (p = 0.003) and higher grades (p = 0.021) as compared with the single group. Furthermore, marked IM and atrophy in adjacent mucosa were more easily seen in the multiple metachronous and synchronous groups as compared with the single group (p < 0.0001). Interestingly, the presence of incomplete type of IM (p = 0.025) and crypt dysplasia (p < 0.0001) in background mucosa was associated with occurrence of metachronous and synchronous lesions following endoscopic resection of GED.Conclusions
The histological features of background mucosa, such as intestinal metaplasia, atrophy, and crypt dysplasia could be used as indicators of occurrence of metachronous and synchronous lesions after endoscopic treatment of GED. 相似文献16.
Suri Chong Dai-Soon Kwak Dhanasekaraprabu Balasubramanian Young Dong Song Young Gon Na Tae Kyun Kim 《The Knee》2017,24(5):925-932
Background
A new device (T-anchor) was developed for ACL reconstruction and is implanted via the outside-in technique using hamstring grafts. The purpose of this study was to compare the T-anchor with the EndoButton Direct.Methods
This study was conducted on 30 cadaveric knees (15 matched pairs). There were two groups of 15 each in the T-anchor and EndoButton Direct groups. After the harvest of grafts, fixation site profile and graft length were measured by loading the grafts onto both devices. They were then tested on a universal testing machine to assess elongation after cyclic loading, load to failure, ultimate load, and mode of failure.Results
The fixation site profile was lower in the T-anchor group than in the EndoButton Direct group (2.3 ± 0.4 mm vs. 4.7 ± 1.0 mm, P < 0.001). The length of the graft-device complex of the T-anchor specimens was longer than that of the EndoButton Direct specimens (125.0 ± 8.9 mm vs. 115.0 ± 8.7 mm, P < 0.001). The mean cyclic elongation was lower for the T-anchor group when compared with the EndoButton Direct group (2.4 ± 0.6 mm vs. 3.9 ± 2.6 mm, P = 0.015). There was no statistically significant difference in ultimate load and load to failure between the T-anchor and EndoButton Direct groups. For mode of failure, the T-anchor fared better (P = 0.013) with all failures attributed to specimens.Conclusions
In this cadaveric study, the new device, T-anchor, performed better than the EndoButton Direct with respect to the above-mentioned study parameters except for ultimate load and load to failure. 相似文献17.
Background
Individual responses to anterior cruciate ligament injury prevention programmes (ACL IPPs) have received little attention. This study examined the effects of an ACL IPP on neuromuscular control and lower limb biomechanics during landing at the group and individual levels.Methods
Sixteen female athletes were randomly allocated to training (n = 8) or control (n = 8) groups. Electromyography, and three-dimensional kinematic and kinetic data were collected during landing at two testing sessions. Repeated measures ANOVA and effect sizes (Cohen's d) examined the effect of the IPP at the group and individual levels. A sub-group analysis comparing the effect of the IPP on ‘high-’ (i.e. large peak knee abduction moment at baseline) versus ‘low-risk’ individuals was also conducted.Results
At the group level; the IPP increased activation of the medial hamstrings prior to landing (p < 0.001; d = 0.264) and the medial gastrocnemius at landing (p < 0.001; d = 0.426), and increased hip external rotation early after initial contact (p < 0.001; d = 0.476). Variable adaptations were seen across individuals within the training group for all variables (p < 0.001). The IPP had a large effect in reducing frontal plane knee moments for ‘high-risk’ individuals (d > 0.91), however these results did not reach statistical significance (p > 0.05).Conclusions
The IPP induced adaptations during landing, however, individual data revealed dissimilar responses to the programme. Individuals displaying a pre-existing high-risk strategy may incur greater benefits from IPPs, yet only if the programme targets the relevant high-risk strategy. 相似文献18.
M. Yaralizadeh P. Abedi F. Namjoyan M. Fatahinia S. Nezamivand Chegini 《Journal de Mycologie Médicale》2018,28(3):419-423
Background
Candida albicans may cause vaginal infections in women. The aim of this study was to compare the antifungal effect of Lawsonia inermis with that of clotrimazole on rats.Methods
A total of 35 female Wistar rats were randomly assigned into 5 groups. Four groups were infected vaginally with C. albicans and one group was not (negative control). The four infected groups received the following treatments: two groups received vaginal creams of 2% or 4% of L. inermis, one group received 1% clotrimazole and one infected group did not receive any treatment (positive control). The hydro-ethanolic henna extract was prepared from the powder of henna leaves using maceration method. Samples were taken for culture from the vaginae of all rats before the treatment, one and two weeks after treatment. An ANOVA test was used to analyze the data.Results
Before the treatment, the mean colony forming units (CFU) was 213.6 ± 10.08 and 334.42 ± 20.32 in the 2% and 4% henna groups, respectively, 312.7 ± 28.32 in the clotrimazole group, 233.85 ± 8.15 in the positive control group, and zero in the negative control group. The mean CFUs were zero for all groups except for the 2% henna and positive control groups (P < 0.001) one week after the treatment and zero in all groups except for the positive control group two weeks after the treatment (P < 0.001).Conclusion
L. inermis (henna) in form of vaginal cream could treat C. albicans infections in female rats; however, 4% henna was more effective and had an effect similar to that of clotrimazole. 相似文献19.
Background
Bulbus Fritillaria cirrhosa D. Don (BFC) has been used in China as a folk medicine for the treatment of cough and asthma for more than 2000 years. The antitussive and antiasthmatic effects of BFC have been reported before, nevertheless its toxicity and safety have not been documented. This study investigated the possible effects of BFC on spindle assembly checkpoint (SAC), mitotic fidelity and genomic stability in human NCM460 colon epithelial cells.Methods
Cells were treated with BFC (0, 20, 40, 80 and 160 μg/ml) for 24, 48 and 72 h and harvested differently according to the biomarkers observed. Mitotic aberrations were assessed by the biomarkers of chromosome misalignment (CMA), chromosome lagging (CL) and chromatin bridge (CB). Frequencies of micronuclei (MN), nucleoplasmic bridge and nuclear bud (NB) in cytokinesis-block micronucleus assay were used as indicators of genomic instability (GIN). SAC activity was determined by anaphase to metaphase ratio (AMR) and the expression of several SAC genes, including CENP-E, Mps1, Bub1, Mad-1, BubR1 and Mad-2.Results
Compared with the control, cells in BFC treated groups (80 and 160 μg/ml) showed: 1) increased AMR (p < 0.05), up-regulated expression of Mps1, Bub1 and Mad-1 (p < 0.05) and down-regulated expression of CENP-E, BubR1 and Mad-2 (p < 0.05); 2) increased frequencies of CMA, CL and CB (p < 0.01); 3) increased incidences of MN and NB (p < 0.01).Conclusions
This study revealed for the first time that BFC causes mitotic aberrations and GIN in human colon epithelial cells and these effects maybe the result of SAC dysfunction. 相似文献20.
Divia Paul A. Ramakrishna Avadhani Subramanyam K. 《Journal of the Anatomical Society of India》2018,67(1):70-76