共查询到20条相似文献，搜索用时 31 毫秒
1.
Azza Saad Shehata Mona Gomah Amer Manal Reda Abd El-Haleem Rehab Ahmed Karam 《Experimental and toxicologic pathology》2017,69(4):203-212
Background
Patients with type I diabetes are at increased risk of osteoporosis even after insulin therapy in adult stage. This study was conducted to compare the efficacy of hesperidin (hesp) therapy versus that of insulin alone in the alleviation of osteoporosis arising from type I diabetes mellitus (T1DM) in young rats.Materials and methods
Hesperidin was administered orally to STZ-induced diabetes. The animals were evaluated morphologically and biochemically and compared with that received daily SC injections of long-acting insulin.Results
Histologically, we observed the degeneration of osteoblasts and osteocytes, decreased collagen fibers, and disturbed bone turn over markers in untreated DM rats. Hesperidin+ insulin supplementation to diabetic rats caused significant improvement of most of the bone histological and morphometric parameters compared with the insulin-treated group. Furthermore, hesp treatment significantly reduced pro-inflammatory mediators TNFα and NF-κB and increased serum biochemical markers of bone turnover, including osteopontin (OPN), osteocalcin (OC) and decreased serum alkaline phosphatase (ALP).Conclusion
These data demonstrated that hesp could be considered to be a beneficial drug for preventing diabetic osteoporosis in growing age. 相似文献2.
S. Sawadogo E. Kafando K. Nébié A.-S. Ouédraogo S. Ouattara H. Dahourou C. Fretz V. Deneys 《Transfusion Clinique et Biologique》2017,24(4):440-448
Background
The National Blood Transfusion Centre, unique operator of blood transfusion in Burkina Faso is engaged into the quality process according to ISO 9001. Therefore, the assessment of customer satisfaction is a main part of its system. Our study conceives “customer satisfaction” as dependant to the perceived service quality based on SERVQUAL model.Objectives
To identify factors associated with the satisfaction of blood products prescribers in order to help decision-makers for continuous improvement of services.Material and methods
We conducted a cross-sectional survey among prescribers of blood components in Ouagadougou, between February 27 and April 30, 2015. We used an anonymous self-administered questionnaire, including 13 items associated to the 5 dimensions of SERVQUAL model. The different satisfaction gaps were calculated and linear regression was used to determine statistical associations with a significance level of 5%.Results
The return rate was 94.5% about the 256 questionnaires distributed. A total of 30% of respondents were satisfied to very satisfied. The overall global gap of satisfaction was ?5.74. The product delivery time, the efficacy and safety of blood products, the medical and clinical support, the pro-activity of the communication, the management of blood products reservation and the satisfaction of needs in blood products were the factors associated with the prescribers’ satisfaction.Conclusion
This first study in blood transfusion services in our context was been useful to assess customer satisfaction and identify the main axes on which targeting priority actions in order to effectively use available resources. 相似文献3.
S. Ouadghiri C. Brick N. Benseffaj O. Atouf M. Essakalli 《Transfusion Clinique et Biologique》2017,24(1):23-27
Purpose of study
The declaration of the recipients adverse reactions (RAR) is one of the field haemovigilance activities. It provides an evaluation of transfusion side effects and thus prevents their appearance. The aim of this study is to analyze, over 14 years, the RAR supports reported in Rabat Ibn Sina hospital.Patients and methods
All of the RAR supports sending to the blood transfusion service were analyzed. The data collected from these supports are: clinical characteristics of the patient, type of incident observed and type of labile blood products (LBP) transfused.Results
A total of 353 RAR were declared with a mean cumulative incidence of 1.7/1000 LBP delivered. Febrile non-hemolytic transfusion reactions represent 72.8% of the RAR declared. The RAR were classified as grade 1 in 87.1% of cases and were secondary to a transfusion of the red cell concentrates in 81.9%. ABO incompatibility was found in four cases (0.02/1000 LBP delivered).Conclusion
The number of RAR reported by Rabat Ibn Sina hospital remains underestimated. Management and traceability RAR and rigorous investigation, under the responsibility of the corresponding haemovigilance contribute to the improvement of transfusion safety. 相似文献4.
A.K. Al-Ghumlas A.K. Al Momen M. Badri A.G. Abdel Gader 《Transfusion Clinique et Biologique》2017,24(2):68-75
Objectives
To determine the long-term trend in platelet consumption in a university hospital.Materials and methods
The annual consumption of platelets concentrate (PC) was analyzed over 23 years (1985–2007) in King Khalid University Hospital (KKUH), Riyadh, Saudi Arabia.Results
The total 23 years consumption was 100,466 units of PC. Consumption went through 3 phases: the first, 1985–1994: the annual consumption increased from 1706 to 5912 which coincided with the increase in the number of patient admissions; the second, 1994-2003:featured a remarkable drop (48.9%) in annual consumption while patient admission remained stable. There was a concurrent decline in platelet consumption and all-cause mortality/patient. Third phase: 2003–2007, the consumption increased to reach 5642 units/year in 2007. The Department of Medicine consumed (52%), followed by Pediatrics (21%), and General Surgery (16%).Conclusion
This audit uncovered evidence of inappropriate platelet consumption that reached 48.9% in the period 1994 to 2003, which coincided with widely publicized HIV scare that dominated blood transfusion during that period. We also found evidence suggesting that reducing platelet transfusion could improve patient outcome. 相似文献5.
Luqman Aribidesi Olayaki Isiaka Abdullateef Alagbonsi Hawau Olaide Abdulkadir Felix Oluwaseyi Idowu 《Journal of the Anatomical Society of India》2017,66(1):67-71
Introduction
Cryptorchidism has been associated with spermatotoxicity and oxidative stress while melatonin is a well-known anti-oxidant. This study investigated the possible ameliorative effect of melatonin on cryptorchidism-induced spermatotoxicity and oxidative stress.Methods
Thirty six male Wistar rats were randomised into sham-operated (n = 18) and bilaterally cryptorchid (n = 18) groups, each of which were subdivided into 3 oral treatment groups (n = 6 rats each) that received normal saline, low dose (4 mg/kg) and high dose (10 mg/kg) melatonin.Results
Cryptorchidism reduced sperm parameters, oestradiol, luteinising hormone, follicle stimulating hormone and glutathione peroxidase activity, but increased testosterone and lactate dehydrogenase activity. The cryptorchidism-induced spermatotoxicity and oxidative stress were ameliorated by low dose of melatonin but exacerbated by its high dose.Discussion
Melatonin’s effect on cryptorchidism-induced spermatotoxicity is dose-dependent. 相似文献6.
Kadir Desdicioglu Mehmet Ali Malas Selen Bahceci Fatma Simsek Ayse Gizem Polat 《Journal of the Anatomical Society of India》2017,66(1):37-42
Introduction
In our study, the aim was to anatomically and histologically investigate the morphometric structures of the branches involved in the sural nerve and sural nerve formation.Method
The study was conducted on 46 lower extremities of 23 fetuses which were obtained from Izmir Katip Çelebi University, Atatürk Training and Research Hospital, with ages from 18 and 32 gestational weeks, without any external pathology or anomaly. During the study period, the posterior-side skin dissection of the lower extremity was performed with the aid of a surgical dissection microscope initially, and the structures forming the sural nerve and the sural nerve were exposed and made visible. Afterwards, sections were taken from these structures for morphometric measurements and histological examination.Results
The mean values and standard deviations of morphometric measurements obtained were determined. Separately, it was determined that there was no statistical difference between right-left sides and genders in morphometric measurements (p > 0.05). The sural nerve was determined to be differentiated into 4 types as A, B, C and D according to the way the nerve branches forming sural nerve join. In addition, differing characteristics pertaining to the sural nerve and branches were determined.Discussion
We are of the opinion that the data obtained in our study will be of use to neurologists, orthopedists and clinicians engaged in this region during interventional procedures. 相似文献7.
Introduction
Protein kinase B (PKB)/Akt is a kinase that is responsive towards insulin-like growth factor (IGF-1) and nerve growth factor (NGF). Detected in the cytosol, Akt is activated by phosphorylation at Thr308/Ser473 residue. The phosphorylated Akt (pAkt) is translocated into the nucleus via the of phosphatidylinositide 3-kinases (PI3K)-Akt pathway. Contradicting results have been reported on the expression of Akt and pAkt in aged animals. The study is conducted to determine the effect of aging on the Akt and pAkt signaling in the L5 dorsal root ganglia (DRG).Methods
Twenty-four female Sprague-dawley rats were sacrificed at 3, 10, 20 and 27 months. L5 DRG was harvested and subjected to immunofluorescence staining.Results
The expression of Akt and pAkt was detected in almost all neurons in all groups. Image analyzer analysis showed that the average intensity of Akt signaling increased from M10 to M27 compared to the M3. However, the average intensity of pAkt signaling was only significantly increased in M27 compared to the younger animals.Conclusion
The expression of Akt and pAkt may indicate that increase activity is taking place in the aged neurons. The activation of Akt and its phosphorylation may be one of the mechanisms in response towards aging process. 相似文献8.
Tuğba Kotil Şengül Şentürk Türkan Sarioğlu Leyla Tapul 《Experimental and toxicologic pathology》2017,69(8):643-648
Aims
Retinoic acid (RA) has a vital importance in order to ensure continuity and morphology in many tissues. Matrix metalloproteinases (MMPs) have significant roles in proliferation, the formation of cancers, and metastasis. In this study the effects of RA on MMP-2 production in cells of rat uterus were investigated.Methods
Twenty-four adult Spraque Dawley rats were divided into two groups, the experimental group was treated with 40 mg/kg/day 13-cis RA for 5 days by gavage. Uterine tissue sections were treated with BrdU and MMP-2 antibodies, evaluated using light microscopy. Tissues were fixed with 2.5% glutaraldehyde and evaluated using transmission electron miroscopy.Results
MMP-2 immunoreactivity decreased in the stromal cells compared with the control group and no staining of MMP-2 was observed in glandular epithelium in the experimental group. BrDU labeling of cells showed significant decrease in RA-treated group versus control group cells. Based on the electron microscopy evaluation, the surface epithelial cells of the experimental group showed vacuolization, and an accumulation of lipofuscin bodies was also observed in the gland epithelium. Cells involving autophagic vacuoles contained excess lipid granules in the entire uterus layers especially localized at the border of the endometrium and myometrium.Conclusion
RA had negative effects on cell proliferation and cell morphology and inhibited MMP-2 expression. 相似文献9.
Tulika Gupta Daisy Sahni Richa Gupta S.K. Gupta 《Journal of the Anatomical Society of India》2017,66(1):58-66
Introduction
There is increasing evidence implicating the active role of melatonin beyond regulation of the human biological clock and reproduction. Its therapeutic use has been extended to neurodegenerative disorders, psychiatric disturbances, memory as well as a host of other neurological manifestations. This study was thus designed to identify regions of brain and retina for the expression of different types of melatonin receptors.Method
Six unfixed brains and 10 retinae were studied. Tissue samples were taken from 14 sites in the brain. Immuno-histochemical staining was done using antibody against Melatonin 1and Melatonin 2 receptors.Result
In the cerebral cortex, MT 1 receptor presence was mostly detected in layers 4 and 5 while MT2 receptors were mapped in all the layers. The frontal and occipital poles were devoid of both the receptors. The suprachiasmatic nuclei of the hypothalamus had immunoreactivity for both MT1 and MT2 while the larger cells of the supraoptic nuclei showed positivity for MT1 receptors. The pyramidal and granule cells of the cerebellar cortex showed the presence of MT2 receptors while the pons and the medullary reticular formation stained positive for MT1 and MT2.Discussion
Differential and comparative characterization of MT1 and MT 2 receptors in different regions of brain and retina has led to virtual creation of a neuroanatomical map localizing potential areas susceptible to interventions specifically targeting melatonergic pathways. 相似文献10.
A. Daurat E. Boudet G. Daurat C. Roger J.-C. Gris V. Tunez M.-C. Gaste J.-Y. Lefrant 《Transfusion Clinique et Biologique》2017,24(2):47-51
Background
In France, blood group determination requires the completion of two samples collected at two different times to detect identity mistake and “wrong blood in tube”. The aims of the present study were: (1) to evaluate the compliance with guidelines and (2) to identify risk factors of non-compliance.Materials and methods
Samples for ABO group determination collected between January 1st and December 15th, 2013 in the University hospital of Nîmes, France were analyzed. An ABO group determination demand was considered non-compliant if more than one tube arrived in the laboratory within ten minutes apart. Between May 1st and June 30th 2014, a self-administered questionnaire was offered to the nurses of the hospital on a random day for each service during this period. The aim was to validate the non-compliance criterion and the identification of risk factors using logistic regression.Results
Among the 16,450 analyzed blood samples, the overall compliance rate was 65.1%. Lower compliance rates were found in the surgical services. Independent risk factors for wrong practice were work overload, surgical service and individual intermediate transfusion frequency.Discussion
More than one third of ABO group determinations did not follow national recommendations, which induces a substantial risk of “wrong blood in tube” and group error. The study revealed major variations among hospital services. Identification of risk factors allows targeted corrective actions. 相似文献11.
P. Veerus R. Kullaste K. Pungas T. Aavik K. Lang 《Transfusion Clinique et Biologique》2017,24(4):404-409
Background
Donating blood in Estonia is non-remunerated and voluntary. Estonian Blood Service system has four independent regional blood centres that are responsible for blood collection, processing, screening and distribution of blood components to hospitals for clinical use.Study design
This research was carried out as a questionnaire survey. A questionnaire was developed to study lapsing first time donors’ (FTD) blood donation experience, intention and willingness to donate again.Methods
A thousand five hundred and forty-six questionnaires were posted to donors who had one successful donation in 2010 and who had not returned to second donation till the year 2012. For data analysis routine statistical methods were used. To evaluate the most appropriate number of classes, based on previous experience and future expectations, latent class analysis was used.Results
There were 453 respondents (29.3%). For the majority of aspects of blood donation experience the emotions were positive. Results of the study suggested that blood collection agencies should intervene to bolster donors’ attitudes, perceived control, and identity as a donor during this crucial post-first donation period.Conclusion
First blood donation seems to have been a positive experience. Reasons leading to stopping blood donation should be studied further. Establishing a donor registry for Estonia would be essential to keep track of donors. 相似文献12.
Objectives
This study aimed to investigate the changes of platelet (PLT) function and coagulation time before and after plateletpheresis donation.Material and methods
The healthy donors were divided into four groups according to the annual number of plateletpheresis donation: 20 times group, 15 times group, 10 times group and 5 times group. The healthy non-blood donors were selected as controls. The donation interval was 14 days. The blood samples were collected before plateletpheresis donation and after 30 min, 7 d, and 14 d of donation for determination of coagulation time, PLT function, plasma protein, serum iron and blood routine change.Results
After 30 min of plateletpheresis donation, the PLT function decreased and the coagulation time was prolonged. However, PLT function recovered to the pre-collection after 7 d of plateletpheresis donation and coagulation time recovered to the pre-collection after 14 d of plateletpheresis donation. Additionally, there was no difference regarding blood coagulation time and PLT function among blood donors and controls. The plasma protein and serum iron levels in 20 times and 15 times groups were within the normal reference range.Conclusion
The frequency of plateletpheresis donation will not affect PLT function, coagulation time, plasma protein and serum iron in donors. 相似文献13.
14.
Background
Massive transfusion is the clinical scenario where the presumed adverse effects of stored blood are expected to be more evident because the whole patient's blood volume is replaced by stored blood.Objective
To analyse the association between age of transfused red blood cells (RBC) and survival in massively transfused patients.Methods
In this retrospective study, clinical and transfusion data of all consecutive patients massively transfused between 2008 and 2014 in a large, tertiary-care hospital were electronically extracted from the Transfusion Service database and the patients’ electronic medical records. Prognostic factors for in-hospital mortality were investigated by multivariate logistic regression.Results
A total of 689 consecutive patients were analysed (median age: 61 years; 65% males) and 272 died in-hospital. Projected mortality at 2, 30, and 90 days was 21%, 35% and 45%, respectively. The odds ratio (OR) for in-hospital mortality among patients who survived after the 2nd day increased with patient age (OR: 1.037, 95% CI: 1.021–1.054; per year P < 0.001), with the number of RBC unit transfused in the first 48 hours (OR: 1.060; 95% CI: 1.038–1.020 per unit; P < 0.001), and the percentage of such RBC stored for more than 28 days (1.010, 95% CI: 1.005–1.018 per percent point; P = 0.01).Conclusion
Mortality after massive transfusion was associated with a higher proportion of old RBCs transfused in the first 48 hours. Other factors associated with poor prognosis were older patient's age and larger volumes of transfused RBCs. 相似文献15.
Vishram Singh D. Krishna Chaitanya B.K.S. Chauhan I. David Victor Kumar 《Journal of the Anatomical Society of India》2017,66(1):78-81
Introduction
The present review is based on the study of various classifications of pneumatization of temporal bone and their comparison. The air cells are classified based on their location in the temporal bone in a radiograph or based on their interpretation by a radiologist or otolaryngologist with the help of different reference structures.Methods
The analysis of pneumatization in temporal bone is done by reviewing research articles related to pneumatization of temporal bone in pig, sheep, macaque and humans published in Pubmed, Sciencedirect, Scopus, and Medline, Indexed journals.Results and discussion
According to classical classification the temporal bone is divided into five regions viz., middle ear, squamomastoid(mastoid), perilabyrinthine, petrous apex and accessory. The cells are named accordingly and they are further classified into various grades by the degree of pneumatization and density of cells present in the respective regionsMost recent work which has been cited in various articles, is done by Han et al. [19] In this pneumatization is classified based on the visualization of various reference structures such as, sigmoid sinus, labyrinth, and internal carotid artery. The lack of consensus among surgeons and otolaryngologists regarding the classification of the pneumatization of temporal bone may be a cause of failure in a few otologic surgeries such as 10 mastoidectomies etc and postoperative care of skull base surgeries. 相似文献16.
M.A. Bani-Ahmad O.F. Khabour M.Y. Gharibeh K.N. Alshlool 《Transfusion Clinique et Biologique》2017,24(4):410-416
Objectives
The reduction in blood viscosity and iron store were proposed to be connected to the reduction in the risk of cardiovascular disease (CVD) among multiple blood donors. Herein, we evaluated the modulation of serum lipids levels in accordance with donation events. Furthermore, atherogenic impacts on the risk of CVD were investigated.Materials and methods
A total of 100 voluntarily male donors were included in the study. Fifty donors were multiple time donors (MTD) and 50 were single time donors (STD). Levels of serum lipids were determined and atherogenic indices including TG/HDL and CHO/HDL ratios were calculated. QRISK2 parameters were determined to evaluate the 10-years risk of developing CVD.Results
Among MTD, there were significantly higher serum levels of triglycerides (TG) and very low-density lipoproteins (VLDL) combined with significantly lower HDL level. These modulations were significantly correlated to the extent of donation. Both CHO/HDL and TG/HDL ratios were also significantly higher among MTD. However, only TG/HDL ratio was strongly correlated to the donation extent even when controlled for age, BMI and smoking status. Despite the significant difference in QRISK2 parameters between study groups, none of these parameters was correlated to the extent of donation when controlling for age, BMI and smoking status.Conclusion
We demonstrate that multiple blood donation is associated with an unfavorable modulation of serum levels of lipids that is influenced by donation extent. This modulation is not associated with an increased risk of CVD but may weakly contribute in a higher risk for coronary heart disease (CHD). 相似文献17.
Mohana Priya Anitha Thomas Ramani Kumar Visalakshi Jeyaseelan Inbam Indrasingh Suganthy Rabi 《Journal of the Anatomical Society of India》2017,66(1):15-19
Introduction
Langerhans cells (LCs), a type of dendritic cells are the professional antigen presenting cells present in the mucosa surfaces. They play an important role in antitumor immune response. The present study aims to find out the morphology and distribution of CD1a positive LCs in normal and squamous cell carcinoma of cervix.Methods
Twenty two normal and eleven ectocervical specimens with squamous cell carcinoma were processed for immunohistochemistry and stained with monoclonal mouse anti-human CD1a (Dako, USA). The morphology of CD1a positive LCs was studied using Olympus BX43 microscope. Morphometric analysis was done using Cellsens imaging analysing software.Results
There was a statistically significant difference in the number of LCs between normal (8 ± 2.76) and squamous cell carcinoma of cervix (5.36 ± 2.88). In the region of lymphatic infiltration both in epithelium and lamina propria, there were more number of LCs and most of the cells lost their dendritic processes in squamous cell carcinoma. 31.77% of the cells had no dendritic processes. The difference in the mean diameters of LCs was statistically significant (p = 0.005) between normal and squamous cell carcinoma of cervix.Discussion
Fewer number of CD1a positive LCs and their loss of dendritic processes in the squamous cell carcinoma of cervix compared to normal cervix indicate that immune responses are suppressed in patients with cancer. 相似文献18.
Esomonu G. Ugochukwu Biose J. Ifechukwude Ude A. Raymond Obun C. Obun 《Journal of the Anatomical Society of India》2017,66(1):26-30
Introduction
To analyse and document anthropometric parameters of the normal human auricle among Nigerians of Cross River State origin as well as to evaluate sexual dimorphism and auricular growth variation among the age groups of this population.Methods
The present study was carried out on 217 adult volunteers made up of 117 females and 100 males, within the age range of 21–60 years; with no evidence of congenital ear anomalies or previous ear surgeries. All subjects were indigenes of Cross River State and were randomly selected across the three senatorial districts of the State. Standard measurement procedures were followed to obtain the following Auricular parameters: Auricular Length (AL), Auricular Width (AW), Lobular Length (LL), Lobular Width (LW), Protrusion at Supra-Aurale (PS) Protrusion at Tragus (PT), Auricular and Lobular Indices.Results
This study revealed that the mean Lobular length and Protrusion at Supra-aurale values are sexually dimorphic at age group of 21–30 while the Auricular length where sexually dimorphic at 21–30 and 31–40 years age groups with higher values noted in males than in females. Other parameters measured were not sexually dimorphic.Discussion
Standard anthropometric methods where employed in carrying out this study, hence this result is recommended to Plastic Surgeons who may need to rectify any auricular abnormalities involving patients from this ethnic group. 相似文献19.
Shereen S. El Shaer Taher A. Salaheldin Nashwa M. Saied Sally M. Abdelazim 《Experimental and toxicologic pathology》2017,69(7):435-441
Background
Cerium oxide nanoparticles have gained much more attention especially in the field of nanomedicine. This work represents cerium oxide nanoparticles as a new prophylactic model for heart failure progression.Objective
To investigate the potential protective effect of cerium oxide nanoparticles on Isoproterenol (ISO)-induced cardiac toxicity in rats.Methods
Cerium oxide nanoparticles (5 ± 1 nm) were synthesized by reverse micelle method and characterized using High Resolution Transmission Electron Microscopy, X-Ray Diffraction and particle size analyzer. The experiments were performed on 96 male Wistar rats. The rats were randomly allocated into eight groups. Namely; two Negative and positive control groups, captopril administered group, Nano-ceria (low dose) group, Nano-ceria (high dose) group, Captopril- Isoproterenol group, Nano-ceria (low dose)-Isoproterenol group and Nano-ceria (high dose)-Isoproterenol group. Cardio toxic rat model was induced by subcutaneous administration of Isoproterenol (ISO) (30 mg/kg) for two consecutive days in adult male rats. Two doses (0.5 and 5 μg/kg/week) of cerium oxide nanoparticles were applied for five weeks and 50 mg/kg/day of Captopril was used as a reference drug. Cardiac marker enzymes, Cortisol and Aldosterone hormones were assessed in serum. Oxidant-antioxidant parameters and histopathological examination in heart tissues were also determined.Results
These dose of nano-ceria, showed a promising ameliorative and prophylactic effect against cardiac toxicity compared to Captopril reference drug. Serum cardiac markers were decreased by noticeable percentage, CK-MB (50% and 57%), LDH (47% and 57.7%), AST (38% and 36.5%) and ALT (33.5% and 30.6%) for both doses respectively, while increased tissues level of the antioxidant enzymes, catalase (48% ? 26%) and superoxide dismutase (64%, 143%).Conclusion
These consistent biochemical and histopathological results suggest that, nano-ceria could be used as effective antioxidant in prophylactic protocols for management of cardiac disorders associated with oxidative stress. 相似文献20.
G. Ibáñez-Cervantes J.M. Bello-López V. Fernández-Sánchez C.A. Domínguez-Mendoza L.I. Acevedo-Alfaro 《Transfusion Clinique et Biologique》2017,24(2):56-61