冠心病患者血浆氧化修饰脂蛋白与血清一氧化氮的变化及相关性研究

氧化修饰低密度脂蛋白（ＯＸ－ＬＤＬ）在动脉粥样硬化性心脑血管疾病发生发展过程中,起着十分重要的作用。内皮细胞损伤被认为是动脉粥样硬化发生的始动环节,ＯＳ－ＬＤＬ对内皮细胞具有毒性作用,且可以直接灭活内皮细胞产生的一氧化氮（ＮＯ）［１］。本文旨在探讨冠...     

高压氧对实验兔海水型呼吸窘迫综合征的预防作用

目的　观察高压氧（ＨＢＯ）对海水淹溺肺水肿（ＰＥ－ＳＷＤ）的预防作用。方法　复制ＰＥ－ＳＷＤ动物模型后,３２ 只兔随机分为ＰＥ－ＳＷＤ对照组、药物治疗组和ＨＢＯ组。对３ 组兔的动脉血气酸碱指标、Ｃａ２＋ 沉淀反应颗粒、ｃ－ｆｏｓ ｍ ＲＮＡ和ｃ－ｊｕｎ ｍ ＲＮＡ 等进行自动检测和定量分析比较,并观察分析３组兔的存活时间和海水型呼吸窘迫综合征（ｓｅａｗ ａｔｅｒ－ｒｅｓｐｉｒａｔｏｒｙ ｄｉｓｔｒｅｓｓ ｓｙｎｄｒｏｍ ｅ,ＳＷ－ＲＤＳ）发生率。结果　ＨＢＯ组兔ＰａＯ２ ,ＳａＯ２ 和ｐＨ ３ 项指标比药物组显著升高（Ｐ＜ ０．０１）,动物存活时间［（４３．０３±７．１９）小时］比药物组［（２３．５８±１．４９）小时］明显延长,Ｃａ２＋ 沉淀反应颗粒,ｃ－ｆｏｓ ｍ ＲＮＡ,ｃ－ｊｕｎ ｍ ＲＮＡ和ＳＷ－ＲＤＳ发生率ＨＢＯ组则明显低于药物组（Ｐ＜ ０．０１）。结论　ＨＢＯ可明显提高ＰａＯ２ 和ＳａＯ２,有效改善低氧血症和代谢性酸中毒,减轻ＰＥ－ＳＷＤ时肺组织的损伤,从而可防止ＰＥ－ＳＷＤ向ＳＷ－ＲＤＳ转化。     

反义核酸对辐射所致白血病的逆转作用

目的探讨ｃ－ｍｙｂ基因反义核酸对辐射所致白血病的逆转作用。方法以脂质体为载体,用人工合成的ｃ－ｍｙｂ基因反义寡核苷酸（ＡＳＯＤＮ）及无关寡核苷酸（Ｎ－ＡＳＯＤＮ）作用于人急性红白血病Ｋ５６２细胞系,以观察Ｋ５６２细胞的增殖、集落形成、ＤＮＡ合成及ｃ－ｍｙｂ癌基因的表达。结果ＡＳＯＤＮ可抑制Ｋ５６２细胞的增殖［作用２４小时,２０μｍｏｌ／Ｌ的ＡＳＯＤＮ的抑制率为（６６．８１±７．５４）％,Ｐ＜０．０１］、集落形成、ＤＮＡ合成［２０μｍｏｌ／Ｌ的ＡＳＯＤＮ作用１２小时、２４小时、４８小时时抑制率分别为（４８．９３±６．７４）％、（６９．５５±１．７８）％和（５７．６２±５．５０）％,Ｐ＜０．０１］及ｃ－ｍｙｂ癌基因的表达;而对照组对Ｋ５６２细胞则无明显影响。结论ｃ－ｍｙｂ基因ＡＳＯＤＮ可逆转Ｋ５６２细胞恶性表型,反义技术对白血病的防治指出了新方向。     

L─ARGININE IMPROVES THE SPLANCHNIC VASODILATATION AND INTESTINAL BLOOD FLOW IN ENDOTOXEMIA

Ｌ┐ＡＲＧＩＮＩＮＥＩＭＰＲＯＶＥＳＴＨＥＳＰＬＡＮＣＨＮＩＣＶＡＳＯＤＩＬＡＴＡＴＩＯＮＡＮＤＩＮＴＥＳＴＩＮＡＬＢＬＯＯＤＦＬＯＷＩＮＥＮＤＯＴＯＸＥＭＩＡＸＩＡＯＮａｎ（肖南），ＣＨＥＮＨｕｉ－ｓｕｎ（陈惠孙），ＨＵＤｅ－ｙａｏ（胡德耀）ＬＵＳ...     

氧惊厥大鼠海马突触体内一氧化氮与钙离子水平的变化

目的：观察高压氧（ＨＢＯ）致氧惊厥时大鼠海马神经元突触体内游离钙离子浓度（［Ｃａ￣（２＋）］ｉ）和作为一氧化氮合成水平指标的ｃＧＭＰ浓度的变化，并观察钙通道阻断剂（苏林碱，ＤＳＬ）和一氧化氮合成酶抑制剂（Ｎ－硝基－Ｌ－精氨酸，ＬＮＮＡ）对氧惊厥的预防作用及其对上述生化参数的影响，同时探讨氧惊厥的机理。方法：实验用ＳＤ大鼠，分两批进行。第一批，观察ＬＮＮＡ和ＤＳＬ对大鼠氧惊厥的保护作用；第二批采用钙荧光指示剂ｆｕｒａ－２／ＡＭ和ｃＧＭＰ放射免疫试剂盒测定了ＨＢＯ致氧惊厥时大鼠海马神经元内［Ｃａ￣（２＋）］ｉ和ｃＧＭＰ浓度。结果：大鼠氧惊厥时，突触体内钙离子和神经元内ｃＧＭＰ均显著升高，分别为对照组的２倍和３倍。预先给ＤＳＬ后，突触体内钙离子和ｃＧＭＰ分别降至５６％和６０％。预先给ＬＮＮＡ后突触体内钙离子和ｃＧＭＰ浓度分别降至氧惊厥组的７０％和３６％。同时，ＣＮＳ氧中毒发生的时间延长，存活率明显增加。Ｌ－精氨酸能明显减弱ＬＮＮＡ对氧惊厥的预防作用。结论：神经元内游离钙离子超载和ＮＯ－ｃＧＭＰ途径激活是造成ＨＢＯ致氧惊厥的重要原因。     

A STUDY OF THE RELATIONSHIP BETWEEN RENAL INJURY SEVERITY AND HIGH ENERGY SHOCK WAVE DOSES APPLIED

ＡＳＴＵＤＹＯＦＴＨＥＲＥＬＡＴＩＯＮＳＨＩＰＢＥＴＷＥＥＮＲＥＮＡＬＩＮＪＵＲＹＳＥＶＥＲＩＴＹＡＮＤＨＩＧＨＥＮＥＲＧＹＳＨＯＣＫＷＡＶＥＤＯＳＥＳＡＰＰＬＩＥＤＪＩＡＮＧＪｕｎ（江军），ＦＡＮＧＹｕ－ｈｕａ（方玉华），ＪＩＮＦｅｎｇ－ｓｈｕｏ（...     

Evaluation of Two Kinds of Spinal Internal Fixation in Clinical Results and Biomechanics

ＥＶＡＬＵＡＴＩＯＮＯＦＴＷＯＫＩＮＤＳＯＦＳＰＩＮＡＬＩＮＴＥＲＮＡＬＦＩＸＡＴＩＯＮＩＮＣＬＩＮＩＣＡＬＲＥＳＵＬＴＳＡＮＤＢＩＯＭＥＣＨＡＮＩＣＳＳＨＥＮＧｅｎ－ｂｉａｏ（沈根标），ＬＵＯＺｈｕｏ－ｊｉｎｇ（罗卓荆），ＣＵＩＨａｉ－ｘｉａｎｇ（...     

Three-Dimensional Motion Measurement of the Atlantoaxial Joint

ＴＨＲＥＥ┐ＤＩＭＥＮＳＩＯＮＡＬＭＯＴＩＯＮＭＥＡＳＵＲＥＭＥＮＴＯＦＴＨＥＡＴ┐ＬＡＮＴＯＡＸＩＡＬＪＯＩＮＴＬＵＹｉ－ｓｈｅｎｇ（卢一生），ＪＩＡＬｉａｎ－ｓｈｕｎ（贾连顺），ＤＩＮＧＺｈｕ－ｑｕａｎ（丁祖泉），ＤＡＩＬｉ－ｙａｎｇ（戴力扬）Ｏ...     

CLINICAL AND EXPERIMENTAL STUDIES ON RENAL INJURIES AFTER EXTRACORPOREAL SHOCK WAVE LITHOTRIPSY

ＣＬＩＮＩＣＡＬＡＮＤＥＸＰＥＲＩＭＥＮＴＡＬＳＴＵＤＩＥＳＯＮＲＥＮＡＬＩＮＪＵＲＩＥＳＡＦＴＥＲＥＸＴＲＡＣＯＲＰＯＲＥＡＬＳＨＯＣＫＷＡＶＥＬＩＴＨＯＴＲＩＰＳＹＦＡＮＧＹｕ－ｈｕａ（方玉华），ＪＩＡＮＧＪｕｎ（江军），ＤＵＷｅｉ－ｇｕｏ（都卫...     

EXPERIMENTAL STUDY OF GUNSHOT INJURY OF THESPINAL COKD：THEMECHANISMANDCLASSIFICATION

ＥＸＰＥＲＩＭＥＮＴＡＬＳＴＵＤＹＯＦＧＵＮＳＨＯＴＩＮＪＵＲＹＯＦＴＨＥＳＰＩＮＡＬＣＯＫＤ：ＴＨＥＭＥＣＨＡＮＩＳＭＡＮＤＣＬＡＳＳＩＦＩＣＡＴＩＯＮＳｉｕＳｈａｏ－ｔｉｎｇ肯少汀ＺｈａｎｇＬｉ－ｒｅｎ张立仁ＧｅＺｈｅｎ－ｓｈａｎ葛振山Ｃｈｅｎｇ...     

Optimization of the system of medical service of students of Cadet Schools of the Ministry of Defense of the Russian Federation

One of the factors of the successful military career guidance Cadet schools students is preserving and promoting their health. Medical support of children and adolescents aged 10-17 years should include the full range of medical and preventive measures defined for this group. The state of providing outpatient care for pupils at the Cadet School in St. Petersburg was studied. These results show that full medical care in accordance with the standards can be based only on children's health clinics. It is important that the organization of medical support pupils cadet schools should be cooperate with civilian health care.     

带状疱疹83例误诊原因分析

带状疱疹是由水痘—带状疱疾病毒引起的皮肤科常见疾病。其主要的病理损害,一是受累神经的严重炎症性浸润,继而导致受侵犯神经节内神经细胞变性、坏死;二是皮肤的水泡。迅速抑制神经节和相应的感觉神经纤维的充血、水肿和坏死,防止粘连形成,达到迅速镇痛、改善皮损,缩短病程及防止后遗症的发生是治疗的关键。因而,尽早明确诊断,     

Estimation of accumulated dose of radiation by method of ESR-spectrometry of dental enamel of mammals

ESR-spectrometry was used to investigate radiation-induced paramagnetic centers in enamel of mammals: carnivores (polar bear and fox), ungulates (reindeer, European bison, moose), and man. Values at half the microwave power saturation of the radiation signal, P_1/2, evaluated at room temperature, was found to range from 16 to 26 mW for animals and man. A new approach to discrimination of the radiation induced signal from the total ESR spectrum of reindeer enamel is proposed. ‘Dose-response’ dependencies of enamel of different species mammals were measured within the dose range from 0.48 up to 10.08 Gy. Estimations of ‘radiosensitivity’ enamel of carnivores and ungulates showed good agreement with radiosensitivity enamel of man by ESR method.     

Analysis of the results of the international comparison of activity measurements of a solution of Fe

The results of an international comparison of activity measurements of a solution of ⁵⁵Fe organized by the BIPM in 2005 are reported and analysed. This exercise, which follows the procedures of the CIPM mutual recognition arrangement to update older comparisons, is a renewal of the comparison organized by the BIPM that took place in 1978. A EUROMET comparison was organized in 1996 specifically to compare activity measurements of a ⁵⁵Fe solution by means of liquid-scintillation techniques. Results of these three comparisons are presented and discussed in this paper.

The radionuclide solution was provided by the NPL, which also distributed the samples to the participants. The activity of the ampoules was measured by 16 laboratories using 12 methods producing 25 results. Some general considerations on uncertainty assessments pertaining to the different techniques used are drawn. The outcome of four different estimators is compared from which the presence of at least one outlier can be confirmed. Further measurements should be made to try to reduce the discrepancy between the results. To date the outcome of the present comparison does not show an improvement to that of the 1996 comparison.     

Evaluation of the results of various technics of non-surgical therapy of varicocele

A new method of non-surgical treatment of varicocele syndrome is described: it consists in sclerotherapy of spermatic vein by trans-femoral percutaneous catheterization with balloon-catheters. In 8 cases venous thrombosis has been induced by direct electric clotting. The techniques and a 6 months follow-up are discussed. It is pointed out that this procedure should be considered as the method of choice for tubular lesions and sub-fertility prophylaxis in young people and in childhood.     

延迟性脾破裂误漏诊原因分析

目的探讨延迟性脾破裂误漏诊原因和预防措施.方法回顾性分析总结12例延迟性脾破裂中的诊断和误漏诊的经验与教训.结果本组延迟性脾破裂的误漏诊5例(41.66%).对多发伤与脾破裂并存可能认识不足,外伤史轻微或伤员隐瞒外伤史,缺乏腹痛-缓解-突然再腹痛的典型病史,缺乏“对冲性脾破裂”力学分析和整体化诊断思路等为其误漏诊的主要原因.结论详细的外伤史和全面系统检查,重视腹以外多发伤掩盖腹内脏器伤及延迟性脾破裂可能.确立外伤-腹内脏器伤-脾破裂整体化诊断思路.不间断地辅以B超检查脾形态学变化和腹内有无积液,腹腔穿刺确定有无血腹、X线胸腹部检查观察左侧胸肋角和膈肌运动情况、必要时CT检查以尽早发现脾包膜下血肿,降低延迟性脾破裂误漏诊率.