地榆、白芷、白蔹在溃疡性结肠炎大鼠中的作用及机制探讨

目的探讨地榆、白芷、白蔹对溃疡性结肠炎大鼠血清中IL-1β、IL-10水平及肠组织中NF-κB表达的影响。方法将48只成年SD大鼠随机分为正常组、模型组、地榆组、白芷组、白蔹组、巴柳氮钠组,每组各8只。除正常组外,其余各组制备大鼠结肠炎模型。造模成功后,各组予相应处理,检测大鼠血清中IL-1β、IL-10含量及大鼠结肠组织中NF-κB p65蛋白水平。结果地榆组、白芷组、巴柳氮钠组大鼠血清中IL-1β水平均明显低于模型组。地榆组、白芷组、巴柳氮钠组血清中IL-10水平较模型组显著升高。与模型组相比,各中药治疗组、巴柳氮钠组大鼠结肠组织中NF-κB p65蛋白表达均明显下调。结论地榆、白芷、巴柳氮钠均可显著降低IL-1β水平,升高IL-10水平,明显下调NF-κB蛋白活性。     

甘肃黄芩苦参金银花体外抑（杀）白色念珠菌试验研究

目的从甘肃黄芩、苦参、金银花中找出抑（杀）白色念珠菌的有效活性成分。方法将甘肃黄芩、苦参、金银花洗净、烘干，粉碎成80目细粉，收集临床分离深部真菌感染白色念珠菌3株，接种于90mm沙保弱平板，在无菌条件下用6mm的打孔器在平板上打孔，每孔填入黄芩、苦参、金银花细粉25．73mg，在药粉中滴加无菌蒸馏水3～5滴。35℃培养24-48h，观察抑菌环大小。结果黄芩细粉的抑菌环直径15～17mm；苦参粉和金银花粉抑菌环为0。结论甘肃黄芩中含有抑（杀）白色念珠菌的有效活性成分，甘肃苦参、金银花中没有。     

尿激酶丹参肝素联合溶解血肿的实验研究

联合应用尿激酶-丹参-肝素对体外血凝块进行溶解试验，测定溶解度和残留液中血红蛋白定量，观察血块中纤维蛋白降解情况。结果显示在设计的各实验组中，以血块形成72小时联合使用尿激酶-丹参-肝素处理24小时组溶解效果最佳，包括残留血块最轻，残留液中血红蛋白最高，病检中显示纤维蛋白降解最完全。说明联合应用尿激酶-丹参-肝素可以促进血凝块的溶解，且血凝块形成72小时后给药溶解效果最佳。从而为临床上选择治疗时机和拟订方案提供初步的实验依据。     

Paeoniae Radix,a traditional Chinese medicine,and CYP2C9 activity

Paeoniae Radix (PR) is a commonly used traditional Chinese medicine. A slight effect of PR on the pharmacokinetics of phenytoin that is mainly metabolised by CYP2C9 has been reported. The aim of this pilot study was to clarify if PR has an effect on losartan oxidation used as a measure of CYP2C9 activity. Three healthy volunteers received a single oral dose of losartan before and after PR treatment. Losartan and E-3174, an active metabolite of losartan, were analysed in 8 hour urine. PR did not seem to have an effect on CYP2C9 activity when the losartan/E-3174 ratios were compared before and after PR treatment (P = 0.56) although a larger study would need to be undertaken to confirm this finding.     

Effects of Paeoniae Radix, a traditional Chinese medicine, on the pharmacokinetics of phenytoin

BACKGROUND: Phenytoin (PHT), one of the most widely prescribed antiepileptic drugs, has been reported to be associated with numerous drug-drug interactions. However, there are far fewer reports about the pharmacokinetic interactions between PHT and traditional Chinese medicines (TCMs). Paeoniae Radix (PR), one of the well-known TCMs, is used as an adjunct in some epileptic patients. OBJECTIVE: In the present work, we studied the influences of PR on the pharmacokinetics of PHT in rats to identify the possible interactions between PR and PHT. METHOD: A single dose of PHT (100 mg/kg) alone or in combination with PR extract (300 mg/kg) was administered by gavage to male SD rats. Serial blood samples of PHT were obtained for up to 24 h post-administration and measured by high-performance liquid-chromatography. The free (unbound) plasma concentrations of PHT were determined by fluorescence polarization immunoassay. The plasma concentrations were used to construct pharmacokinetic profiles by plotting drug concentration-time curves. All data were subsequently processed by the computer program WINNONLIN. Statistical comparisons of pharmacokinetic parameters were performed with the unpaired Student t-test. RESULTS: The mean maximum plasma concentration of PHT was attained 2 h after oral administration of PHT alone and 4-6 h after oral administration of PHT in combination with PR. The plasma level of PHT declined with a half-life of 5.38 h after PHT alone and 4.03 h after PHT and PR given together. No statistically significant differences were obtained in most of the pharmacokinetic parameters (Cmax, AUC, t1/2, MRT and CL/F) and protein binding rates of PHT between the two treatments. However, significant differences in Tmax and Vd/F between groups were noted. CONCLUSION: The significant increase in Tmax indicated that simultaneous oral administration of PR delayed the absorption of PHT. The delayed absorption of PHT might lead to its slow onset of clinical effect. There were no significant differences in Cmax, AUC, t1/2, MRT and CL/F of PHT between the two groups, showing that PR could not significantly affect the extent of absorption, metabolism and elimination of PHT. No significant difference in protein binding rate was found, indicating that PR might not significantly alter the protein binding of PHT. While a significant decrease in Vd/F was noted, the mechanism underlying the apparently decreased Vd/F of PHT influenced by PR needs further study.     

柴胡黄芩配伍不同提取部位对大鼠急性酒精性肝损伤保护作用的比较

目的：以湿热气滞瘀阻为病机，从中医整体观念、肝胃同冶出发，观察具有疏肝理气。清热燥湿功效的中药柴胡、黄芩不同提取部位对大鼠急性酒精性肝损伤的防治作用。 方法：实验于2004-08／12在河南中医学院中药学实验室完成，取健康Wistar大鼠84只，分为正常组、横型组、石油醚组。乙酸乙酯组，正丁醇组。水提取物组。当飞利肝宁胶囊组共7组，每组12只。柴胡黄芩药液制备：柴胡120g、黄芩240g混匀，经700mL／L乙醇两次连续回流提取，过滤。浓缩成浸膏；将浸膏依次用石油醚、乙酸乙酯、水饱和的正丁醇萃取，回收有机溶剂，低温烘干；用50mL/L聚山梨酯80配成浓度为0．9g／mL的溶液。使用时将上述药液接所需浓度稀释。当飞利肝宁胶囊，以当药、水飞蓟提取物配伍组成，四川美大康药业股份有限公司生产（批号040102）。各组大鼠除正常对照组（以等体积的蒸馏水灌胃）外，其余各组均予560mL／L乙醇造急性酒精性肝损伤模型。自造模第1天起，正常组和模型组灌服等体秘的蒸馏水（含聚山梨酯80），其余各组分别灌胃给予相应的药液，剂量皆为12g／kg（10mL／kg），2次／d，连续10d。通过对一般状况、脏器指数的比较，胃粘膜一氧化氮、内皮素含量的测定，胃组织病理形态学变化观察，从整体调节角度阐述柴胡黄芩配伍抗急性酒精性肝损伤的作用。 结果：灌胃操作不当死亡3只，酒精中枢抑制作用和消化系损伤死亡11只，晟终共70只纳入结果分析。正常组12只，模型组9只，石油醚组9只。乙酸乙酯组10只，正丁醇组10只，水提取物组10只，利肝宁胶囊组10只。①与正常组比较，模型组大鼠脾脏指数、胸腺指数、肾上腺指数均显著降低（t=4．830，2．903，4．544，P〈0．01）。正丁醇组与模型组比较，胸腺指数、肾上腺指数均有显著升高（拉3．326，2．847，P〈0．01-0．05）；利肝宁胶囊组与模型组比较，脾脏指数、胸腺指数、肾L腺指数均明显升高（t=3．430 , 4．842，4．436，P〈0．01）。②各组大鼠之间胃组织一氧化氮的音量无显著变化。模型组与正常对照组比较，胃组织内皮素含量显著升高（t=4．292，P〈0．01）；正丁醇组和利肝宁胶囊组能降低胃组织中升高的内皮素含量，与模型组比较，趋于正常（t=2．214，2．272，P〈0．05）。③模型组大鼠胃粘膜下层充血。粘膜上皮细胞近胃腔部腺上皮脱落。各给药组胃粘膜病理形态学改变明显恢复，尤以正丁醇组和利肝宁胶囊组作用显著，基本趋于正常组织。 结论：柴胡黄芩配伍后可改善机体一般情况，调整机体的内分泌和免疫系统。尤以正丁醇萃取作用显：酱，降低乙醇引起的内皮素水平升高对胃粘膜起到保护作用。     

Lack of pharmacokinetic interaction between valproic acid and a traditional Chinese medicine, Paeoniae Radix, in healthy volunteers

BACKGROUND AND OBJECTIVE: In addition to the standard antiepileptic drugs, traditional Chinese medicines (TCMs) are used for the treatment of epilepsy in oriental countries. The interactions between antiepileptic drugs and TCMs represent a potential problem in clinical application. Because valproic acid (VPA), one of the most widely prescribed antiepileptic drugs, may be administered concomitantly with Paeoniae Radix (PR), one of the famous TCMs, in some epileptic patients, the present study was conducted to evaluate the influences of PR on the pharmacokinetics of VPA. METHOD: The pharmacokinetics of VPA were investigated in a randomized, open-label, two-way crossover study. Six healthy volunteers received the following treatments in a crossover design: (i) 1.2 g extract powder of Paeoniae Radix once daily for 7 days and one 200 mg VPA gastro-resistant tablet on day 7 and (ii) one 200 mg VPA gastro-resistant tablet alone on day 7. Serial plasma samples were obtained on day 7. Total and free (unbound) VPA plasma concentrations were determined by fluorescence polarization immunoassay (FPIA). Safety measures included laboratory tests (haematology, serum chemistry and urinalysis) and adverse event monitoring. Statistical comparisons of pharmacokinetic parameters were performed with the Student paired t-test. RESULTS: Overall clinical safety was satisfactory. The mean maximum plasma concentration of VPA was attained at within 6 h after oral administration of VPA alone and 3-4 h after oral administration of VPA in combination with PR. The plasma level of VPA declined with a half-life of 11.71 and 11.91 h, respectively. No statistically significant difference was obtained in any of the pharmacokinetic parameters (Tmax, Cmax, AUC, t1/2, MRT, CL/F and Vd/F) of VPA between the two treatments. Also, there was no significant difference in the protein binding rates of VPA. CONCLUSION: PR did not significantly affect the absorption, distribution, metabolism and elimination of VPA in healthy volunteers.     

柴胡黄芩配伍不同提取部位对大鼠急性酒精性肝损伤保护作用的比较

目的:以湿热气滞瘀阻为病机,从中医整体观念、肝胃同治出发,观察具有疏肝理气,清热燥湿功效的中药柴胡、黄芩不同提取部位对大鼠急性酒精性肝损伤的防治作用。方法:实验于2004-08/12在河南中医学院中药学实验室完成,取健康Wistar大鼠84只,分为正常组、模型组、石油醚组,乙酸乙酯组,正丁醇组,水提取物组,当飞利肝宁胶囊组共7组,每组12只。柴胡黄芩药液制备:柴胡120g、黄芩240g混匀,经700mL/L乙醇两次连续回流提取,过滤,浓缩成浸膏;将浸膏依次用石油醚、乙酸乙酯、水饱和的正丁醇萃取,回收有机溶剂,低温烘干;用50mL/L聚山梨酯80配成浓度为0.9g/mL的溶液,使用时将上述药液按所需浓度稀释。当飞利肝宁胶囊,以当药、水飞蓟提取物配伍组成,四川美大康药业股份有限公司生产(批号040102)。各组大鼠除正常对照组(以等体积的蒸馏水灌胃)外,其余各组均予560mL/L乙醇造急性酒精性肝损伤模型。自造模第1天起,正常组和模型组灌服等体积的蒸馏水(含聚山梨酯80),其余各组分别灌胃给予相应的药液,剂量皆为12g/kg穴10mL/kg雪,2次/d,连续10d。通过对一般状况、脏器指数的比较,胃粘膜一氧化氮、内皮素含量的测定,胃组织病理形态学变化观察,从整体调节角度阐述柴胡黄芩配伍抗急性酒精性肝损伤的作用。结果:灌胃操作不当死亡3只,酒精中枢抑制作用和消化系损伤死亡11只,最终共70只纳入结果分析。正常组12只,模型组9只,石油醚组9只,乙酸乙酯组10只,正丁醇组10只,水提取物组10只,利肝宁胶囊组10只。①与正常组比较,模型组大鼠脾脏指数、胸腺指数、肾上腺指数均显著降低(t=4.830,2.903,4.544,P<0.01)。正丁醇组与模型组比较,胸腺指数、肾上腺指数均有显著升高穴t=3.326,2.847,P<0.01～0.05雪;利肝宁胶囊组与模型组比较,脾脏指数、胸腺指数、肾上腺指数均明显升高穴t=3.430,4.842,4.436,P<0.01雪。②各组大鼠之间胃组织一氧化氮的含量无显著变化。模型组与正常对照组比较,胃组织内皮素含量显著升高穴t=4.292,P<0.01雪;正丁醇组和利肝宁胶囊组能降低胃组织中升高的内皮素含量,与模型组比较,趋于正常(t=2.214,2.272,P<0.05)。③模型组大鼠胃粘膜下层充血,粘膜上皮细胞近胃腔部腺上皮脱落。各给药组胃粘膜病理形态学改变明显恢复,尤以正丁醇组和利肝宁胶囊组作用显著,基本趋于正常组织。结论:柴胡黄芩配伍后可改善机体一般情况,调整机体的内分泌和免疫系统,尤以正丁醇萃取作用显著,降低乙醇引起的内皮素水平升高对胃粘膜起到保护作用。     

参附注射液在重症感染性休克中的疗效观察

感染性休克的治疗一直是临床较为棘手的问题,近几十年来,临床对感染性休克的治疗虽然取得了很大进步,但并没有从根本上提高感染性休克患者的生存率,短期内病死率仍然超过50%[1]。作者在ICU监护及治疗中对收治的3例重症感染性休克患者,在常规抗休克治疗无效的情况下,短期内持续应用参附注射液进行治疗,收到良好的效果。现分析报告如下。1病例资料【例1】男,66岁。因突发呼吸、心跳骤停,心肺复苏术后入住ICU。在治疗过程中并发肺部金黄色葡萄球菌及真菌感染,对多种抗生素耐药,仅对万古霉素及两性霉素B敏感。治疗中须大剂量多巴胺(>25μg·k…     

甘肃黄芩中黄芩苷、黄芩苷元、黄芩苷盐对白色念珠菌的抑制

目的找出甘肃黄芩中抑制白色念珠菌的有效活性成分。方法将甘肃黄芩洗净、烘干、粉碎成（80目）粉,提取其中黄芩苷、黄芩苷元,制成粉末,黄芩苷盐由黄芩苷与NaOH反应生成。收集临床分离深部感染真菌白色念珠菌3株,接种于90mm沙保弱平板,在无菌条件下,用6min的打孔器在平板上打孔,每孔填充黄芩苷、黄芩苷元、黄芩苷盐药粉43．4mg,在药粉中滴加无菌蒸馏水2～3滴,35℃培养24～48h,观察抑菌环大小。结果黄芩苷元、黄芩苷盐药粉对3株白色念珠菌抑菌环直径分别为（18．7&#177;2．9）mm、（12．8&#177;1．5）mm,黄芩苷药粉抑菌环直径为0。结论甘肃黄芩中抑制白色念珠菌的有效活性成分是黄芩苷元,黄芩苷与NaOH反应产物黄芩苷盐亦有效,黄芩苷无效。     

HPLC法测定刺五加中刺五加苷B的含量

目的:对不同生长年限、不同药用部位的刺五加中刺五加苷B进行了含量测定,为合理开发、利用这一药用植物资源提供了理论依据。方法:采用高效液相色谱法,研究了不同生长年限、不同药用部位的刺五加中的刺五加苷B含量的变化规律。结果:3年生的刺五加中刺五加苷B的含量高于1年生的剌五加,且均以茎皮中含量较高,茎中含量次之,根中最低。结论:对刺五加药材的合理采收提供了参考依据。     

黄芪注射液对体外血管新生的影响

背景:血管新生受生长因子的影响,黄芪可与血管内皮生长因子具有协同促血管新生的功效,但机制尚不明确。目的:通过与单一血管内皮生长因子干预比较,观察中药黄芪对体外血管新生的作用机制。方法:将黄芪注射液及血管内皮生长因子作用于SD大鼠胸主动脉内皮细胞,应用细胞增殖、细胞迁移、小管形成实验观察黄芪注射液对体外血管新生的促进作用,用Western blot实验检测血管内皮生长因子的表达。结果与结论:黄芪能明显促进大鼠胸主动脉内皮细胞的增殖(P<0.01),迁移的细胞数增加(P<0.01),胸主动脉内皮细胞的小管形成数增加(P<0.01),并明显促进内皮细胞血管内皮生长因子的表达(P<0.01)。说明黄芪能明显促进内皮细胞增殖、细胞迁移、小管形成,具有显著的促进体外血管新生作用,其机制可能是通过增加血管内皮生长因子的表达而实现的。     

黄芪注射液对体外血管新生的影响

背景：血管新生受生长因子的影响,黄芪可与血管内皮生长因子具有协同促血管新生的功效,但机制尚不明确。目的：通过与单一血管内皮生长因子干预比较,观察中药黄芪对体外血管新生的作用机制。方法：将黄芪注射液及血管内皮生长因子作用于SD大鼠胸主动脉内皮细胞,应用细胞增殖、细胞迁移、小管形成实验观察黄芪注射液对体外血管新生的促进作用,用Western blot实验检测血管内皮生长因子的表达。结果与结论：黄芪能明显促进大鼠胸主动脉内皮细胞的增殖（P〈0.01）,迁移的细胞数增加（P〈0.01）,胸主动脉内皮细胞的小管形成数增加（P〈0.01）,并明显促进内皮细胞血管内皮生长因子的表达（P〈0.01）。说明黄芪能明显促进内皮细胞增殖、细胞迁移、小管形成,具有显著的促进体外血管新生作用,其机制可能是通过增加血管内皮生长因子的表达而实现的。     

热敷法降低静脉滴注刺五加注射液不良反应的临床观察

目的：降低刺五加注射液在输液过程中的血管刺激症状。方法：随机将100例静脉点滴刺五加注射液的患者分为2组，各50例，实验组在输液过程中用热敷法，对照组用传统方法，比较两组不良反应的发生率。结果：实验组具有血管刺激症状8例（16％），对照组34例（68％），两组差异有显著性（P〈0．001）。结论：热敷法能降低刺五加注射液的血管刺激症状。     

Antitumor component recognition from the Aconiti Lateralis Radix Praeparata and Glycyrrhizae Radix et Rhizoma herb pair extract by chemometrics and mean impact value

The purpose of this research is to recognize the active antitumor components from the mixed pair extract of Aconiti Lateralis Radix Praeparata (Fuzi in Chinese) and Glycyrrhizae Radix et Rhizoma (Gancao in Chinese) using chemometrics and mean impact value (MIV) methods. Firstly, 30 common components of 31 different samples were analyzed quantitatively and qualitatively by HPLC-UV and UPLC-Q-TOF tandem mass spectrometry, respectively. Meanwhile, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays were used to test the inhibition activities of the 31 different samples against HeLa cells. Then a back propagation (BP) neural network, support vector regression (SVR), and two optimization algorithms – genetic algorithm (GA) and particle swarm optimization (PSO) – were applied to construct composition–activity relationship (CAR) models for the Fuzi–Gancao extract. Based on the optimal CAR model, the MIV was introduced to evaluate the contribution of each individual component to the anticancer efficacy of the extract. Results indicated that the SVR-PSO model best depicted the complex relationship between the chemical composition and the inhibition effect of a Fuzi–Gancao extract. The 30 common components were ranked by their absolute MIVs, and the top 8, which corresponded to peaks 17, 25, 22, 13, 23, 28, 5, and 7 in the chromatogram, were tentatively deemed to be the main antitumor components. The integrated strategy shows a novel and efficient approach to understanding the potential contributions of components from complicated herbal medicines, and the identified results suggest certain directions for screening and research into new antitumor drugs.

CAR models for the Fuzi–Gancao herb pair were constructed by BP, SVR, GA and PSO, and used to fit experimental data. The main active antitumor components were recognized from MIVs based on the optimal CAR model.     

黄芪注射液对严重脓毒症患者的疗效观察

目的 观察严重脓毒症患者用黄芪注射液治疗前后的临床疗效.方法前瞻性研究,收集2006-2008年人住浙江大学医学院附属第一医院ICU内严重脓毒症患者50例,排除恶性肿瘤,妊娠患者,随机分为黄芪组(n=30)和对照组(n=20),两绀患者资料具有可比性.在相同基础治疗条件下,黄芪组增加黄芪注射液治疗,分别于治疗前、治疗第7天和第14天计算急性生理和慢性健康评分(APACHEⅡ评分),并于28 d统计两绀患者的死亡率.再于清晨同一时间空腹抽取外周静脉血,直接免疫荧光法检测血 CD4,CD8,CD4/CD8,NK细胞水平,酶联免疫吸附法检测IL-1βIL-6,TNF-α水平.采用SPSS 11.5统计软什包进行数据统计.结果 治疗后第14大黄芪组APACHE Ⅱ评分为(20.73±5.06),而埘照组为(35.00±3.70),两组APACHF Ⅱ评分差异具有统计学意义(P<0.05),28 d死亡率黄芪组为25.3%,对照组为41.6%,两组比较差异具有统计学意义(P<0.05).黄芪组治疗后14 d血CD4,CD4/CD8,NK细胞水平回升,分别为(34.11±14.09)%,(2.05±0.98)%,(28.83±8.08)%,较对照绀差异具有统计学意义(P<0.01),而CD8,IL-6及INF-α水平下降,分别为(20.84±9.84)%,(710.87±313.52)pg/mL,(14.63±30.38)pg/mL,较对照组差异具有统计学意义(P<0.01).结论 脓毒症患者存在免疫失衡并且影响其病情及预后,黄比注射液治疗有助于渊整这种免疫失衡并且可改善患者临床疗效.     

黄芪注射液对严重脓毒症患者的疗效观察

Objective To observe the effects of Radix Astragali injection on severe septic patients. Method This was a prospective study. Fifty severe septic patients admitted in ICU from 2006 to 2008 were col-lected, malignent disease like carcinoma, pregnant ones were excluded. They were randomly divided into two groups: Radix Astragali injection treatment group (n = 30) and control one (n = 20). The data of the two groups were comparable. With the same basic therapy, the experimental group treated with additional Radix Astragali in-jection. Then, APACHE Ⅱ scores were calculated on day 0(before therapy), day 7 and day 14 after treatment with Radix Astragali injection, and the 28th day mortality was also calculated for both groups. Blood CD4, CD8, CD4/CD8, NK-cell expression level was measured by direct immunofluorescence, IL-1β, IL-6, TNF-α level was tested by ELASA. SPSS 11.5 software, was used for statistic. Results The APACHE Ⅱ scores in Radix Astragali treatment group were 20.73±5.06, while in control groups were 35.00±3.70 after treatment for 14 days, and mortality in the 28th day was 25.3% and 41.6% respectively, there were significant deferences between the two groups (P < 0.05). At 14 days after treatment, the level of CD4, CD4/CD8 and NK-cell in the Radix Astragali treatment group was significantly increased, with (34.11±14.09) %, (2.05±0.98) %, (28.83±8.08) %, re-spectively, and compared with control group, there were significant differences (P < 0.01). Though, the level of CD8, IL-6 and TNF-α was showed (20.84±9.84) %,(710.87±313.52) pg/mL,(14.63±30.38) pg/mL respectively, significantly decreased after treatment with Radix Astragali injection, there were also significant dif-ferences between the two groups (P < 0.01). Conclusions Radix Astragali injection can improve the immunity disorder of severe septic patients, it will be a hopeful therapy for severe sepsis.     

黄芪对大鼠应激性胃溃疡的预防作用

目的观察黄芪注射液对应激状态下大鼠胃黏膜损伤有无保护作用。方法采用水浸束缚应激方法复制大鼠应激性溃疡模型。将40只健康大鼠随机分为5组：对照组、模型组、黄芪10g／kg、20g／kg组和硫糖铝500mg／kg给药组。分别连续灌胃给予黄芪或硫糖铝5d后复制模型,6h后取胃计算溃疡指数,采用光镜观察大鼠胃组织病理改变。结果水浸束缚应激致应激性溃疡形成,胃黏膜出现广泛的糜烂、出血。黄芪和硫糖铝均可显著降低溃疡指数（P〈0．01）,其中黄芪20g／kg疗效最佳,优于硫糖铝500mg／kg（P〈0．05）。黄芪可显著减轻大鼠胃黏膜细胞损伤程度和毛细血管及其周围间质的水肿。结论黄芪对应激性溃疡大鼠具有显著的胃黏膜保护作用。     

附片水煎制剂中乌头类生物碱水解物含量测定研究

目的考察用乌头碱水解物、新乌头碱水解物、次乌头碱水解物的含量作为附片水煎剂及附片质量控制指标的可行性。方法采用高效液相色谱分析法,色谱柱为ShimpackCLC-ODS,以甲醇-乙酸铵溶液（O．2mol／L）（200：210）为流动相,流速1．0mL／min,检测波长为241nm,对乌头碱新,乌头碱、次乌头碱水解物的含量测定进行研究。结果3种水解物检测方法测得水解物在进样范围有良好线性关系,重现性好,稳定性好,生药提取时间宜选定为40min,不同批号的附片中3种水解物含量差别较大。结论此方法简便,使用新乌头碱、乌头碱、次乌头碱的水解物作为含附片制剂的质量控制指标是可行的。     

黄芪注射液对非霍奇金淋巴瘤患者血清中存活素水平的影响

【目的】探讨黄芪注射液对非霍奇金淋巴瘤（NHL）患者血清中存活素（Survivin）水平的影响。【方法】80例NHL患者随机分为两组，每组40例，治疗组采用黄芪注射液配合CHOP方案治疗，对照组单用CHOP方案化疗。治疗前后检测患者血清中Survivin浓度。评价两组患者的近期疗效、生存质量、毒副反应。【结果】治疗后与对照组比较，治疗组患者血清中Survivin浓度和毒副反应明显降低，近期疗效和生存质量显著提高。【结论】黄芪注射液可能通过协同CHOP方案降低NHL患者血清中Survivin浓度，提高NHL的治疗效果，改善生存质量，降低机体的毒副反应。