HLA-B51与白塞病相关性研究

目的 探讨HLA-B51等位基因和白塞病的相关性。方法 应用PCR-SSP（序列特异性引物）技术对61例中国汉族白塞病患者及100例正常人对照的HLA-B5101 ～ HLA-B5109等位基因进行检测。结果 与对照组（11例阳性,11%）相比,白塞病组HLA-B51频率（18例阳性,29.5%）明显增高（χ2 = 8.79,P < 0.01,RR = 3.39）,且白塞病组与对照组中HLA-B51阳性者均为HLA-B5101等位基因。HLA-B51阳性者中男15例,女3例,HLA-B51阴性者中男22例,女21例,两组男女比例差异有统计学意义（P < 0.05）;HLA-B51阳性者平均发病年龄为（28.4 ± 10）岁,HLA-B51阴性者（37.3 ± 12）岁,二者间差异亦有统计学意义（P < 0.05）。在其他各临床表现中,包括类型、病程、皮损、生殖器溃疡、眼部损害、针刺反应、关节受累及皮损类型等均未见到二者差异有统计学意义。结论 HLA-B5101等位基因与中国汉族白塞病发病相关,并与患者性别及发病年龄有明显相关性。     

HLA—Cw＊0602与银屑病的关联研究

为研究HLACw*0602与DRB1*07两个基因在银屑病发病中的作用,采用PCRSSP基因分型方法,用一对引物扩增DRB1*073对引物扩增Cw*0602,扩增引物在含溴乙锭的1%琼脂糖凝胶电泳后,紫外灯下定型。结果,病人组和对照组Cw*0602阳性数分别为63.0%和7.4%,RR=21.3P<0.00001DRB1*07为54.3%和18.5%,RR=5.2P<0.0003。表明HLACw*0602是与银屑病关联的基因之一。     

HLA—DRB1等位基因多态性与白塞病的相关性研究

目的：分析HLA—DRB1等位基因多态性与白塞病（BD）的相关性。方法：应用LABTypeTMSSO法对43例BD患者及120例正常对照组的HLA—DRB1等位基因进行检测。结果：与正常对照组相比,BD患者HLA—DRB1＊14基因频率明显增高（P〈0．05）,而HIA—DRB1＊15基因频率明显降低（P〈0．05）。HLA-DRB1等位基因与BD的临床表现具有一定的相关性。结论：HLA—DRB1＊14可能是BD的易感基因,HLA—DRB1＊15可能是BD的保护性基因。     

HLA—DQB1基因与汉族天疱疮的相关性研究

目的：探讨HLA－DQB1基因在天疱疮易感性中的作用。方法：用序列特异性引物－聚合物链反应（PCR－SSP）方法，对59例寻常型（PV）患者、37例红斑型天疱疮（PE）患者及53例正常对照者进行了HLA－DQB1等位基因的分型，并分析了DQB1基因在两组中的分布。结果与结论：与正常对照组比较，PV与PE患者组中HLA－DQB1*0302、DQB1*0503基因频率明显增高。本研究结果提示，HLA－DQB1*0302、DQB1*0503基因是我国汉族天疱疹易感性的标志。     

HLA-A等位基因与白塞病临床表现的相关性研究

目的：探讨HLA- A等位基因与白寒病（BD）临床表现的相关性。方法：应用LABType^TN SSO技术（又称序列微珠综合分析实验系统）对42例BD患者及116人正常对照者的HLA- A等位基因进行检测。结果：与正常对照组相比，有毛睫炎样皮损的BD患者中HLA- A＊02等位基因频率明显降低（P〈0．05），有关节、神经系统受累的BD患者的HLA- A＊29等位基因频率均明显增高（均P〈0．01），有反复口腔溃疡家族史的BD患者中HLA- A＊23等位基因频率明显增高（P〈0．05）。结论：HLA-A等位基因频率与BD的临床表现有一定的相关性。     

HLA-DRB1等位基因多态性与白塞病的相关性研究

目的:分析HLA-DRB1等位基因多态性与白塞病(BD)的相关性.方法:应用LABTypeTMSSO法对43例BD患者及120例正常对照组的HLA-DRB1等位基因进行检测.结果:与正常对照组相比,BD患者HLA-DRB1*14基因频率明显增高(P<0.05),而HLA-DRB1*15基因频率明显降低(P<0.05).HLA-DRB1等位基因与BD的临床表现具有一定的相关性.结论:HLA-DRB1*14可能是BD的易感基因,HLA-DRB1*15可能是BD的保护性基因.     

大疱性类天疱疮与HLA—A、B等位基因的相关性

目的：确定山东汉族大疱性类天疱疮（BP）与HLA—A、B等位基因的相关性。方法：运用聚合酶链反应-序列特异性引物寡核苷酸探针杂交（PCR—SSOP）方法,对山东地区43例汉族BP患者和125例健康对照进行了HLL—A、B等位基因分型。结果：BP患者组HIA—A＊24频率高于对照组（P：0．033,Pc〉0．05）;HLA—A＊33、B＊44在患者组中频率低于正常对照组（P值分别为0．040和0．024,但Pc值均〉0．05）。结论：大疱性类天疱疮的遗传易感基因可能与HLA—A、B等位基因无相关性。     

HLA抗原与白塞病相关的研究

ＨＬＡ抗原与白塞病相关的研究冯高章，李莹，马烈，于淞，田丁，王黎曼，峰下哲哈尔滨医科大学附属二院皮肤科（邮政编码１５００８６）白塞病（Ｂｅｈｃｅｔｄｉｓｅａｓｅ，ＢＤ）的病因迄今尚无定论，近代学者对该病进行了众多的ＨＬＡ的群体研究，发现ＨＬＡ系统与Ｂ...     

白塞病患者血清瘦素水平的检测及其意义

目的:探讨瘦素在白塞病发病机制中的作用。方法:采用放射免疫分析法对41例白塞病患者及30例正常对照组血清瘦素水平进行了检测。结果:41例白塞病患者血清瘦素水平(7.37±6.53)ng/mL比正常对照组(4.44±2.56)ng/mL,显著升高(P<0.05);活动期白塞病患者比稳定期白塞病患者及正常对照组瘦素水平显著升高(均P<0.01);有内脏受累白塞病患者比无内脏受累白塞病患者及正常对照组瘦素水平显著升高(均P<0.01);有关节受累白塞病患者比无关节受累白塞病患者及正常对照组瘦素水平亦升高(P<0.05、P<0.01);分组比较结果男、女白塞病患者各组与未分组前的结果一致。结论:白塞病患者体内存在瘦素水平的异常,这种异常可能与白塞病的发生与发展有关。     

58例白塞病回顾性分析

目的：总结和分析白塞病的临床表现及诊治经验。方法：回顾性分析58例白塞病患者。结果：本组白塞病以女性、慢性型多见，好发于青壮年。21例急性型发病诱因以上呼吸道感染为主（15例，占71．43％），应注意病毒或细菌感染在急性型发病机制中的作用。临床表现以口腔、皮肤、生殖器、眼和关节受累常见。结论：针刺反应对本病特异性高。皮肤外伤后创面延迟愈合（23例，占39．66％）对诊断有重要价值。     

Behcet病21例临床分析及眼内超声表现

目的：探讨Behcet病的临床特征以及超声检查对眼部损害的诊断价值。方法：回顾性分析了21例Behcet病的临床资料，包括8例眼部损害患者（13只眼）的超声图像特点。结果：口腔溃疡、外生殖器溃疡、皮肤损害、眼部病变是本组Behcet病的基本临床特征，以口腔溃疡最常见。前房积脓，玻璃体雪球状浑浊，伴粟粒样强回声光点及纤维光带形成等是Behcet病的主要超声表现。结论：某些口腔溃疡反复发作常规治疗效果欠佳时，可能是Behcet病的早期表现。超声检查可对Behcet病眼部损害以及本病的早期诊断提供有用的影像学依据。     

HLA-B51抗原与白塞病临床特征的相关性研究

目的 :　探讨HLA B5 1与白塞病 (BD)临床特征的相关性。方法 :　应用微量淋巴细胞毒试验 ,对 2 7例BD患者和 30例健康人的HLA A和HLA B抗原进行研究。结果 :　BD患者和对照组HLA -B5 1的阳性频率分别为 6 3%和 16 .7% (χ2 =12 .9,P <0 .0 0 1,Pc <0 .0 5 ,RR =8.5 )。其中 ,HLA -B5 1阳性的男性患者的相对危险度 (RR)高于女性患者 (分别为 17.39和 4 .2 9) ,两者相比存在差异 (χ2 =4 .2 0 ,P <0 .0 5 )。未发现BD患者的其他临床特征与HLA B5 1有相关性。结论 :　提示HLA B5 1是BD的易感基因 ,并与男性患者密切相关     

Association of HLA subtype DRB10407 in Colombian patients with actinic prurigo

BACKGROUND: Human leukocyte antigen (HLA) DRB1*0407 had been associated with actinic prurigo in different populations. This class II HLA-DR subtype had not been studied in Colombia. OBJECTIVE: The objective of this study was to establish whether there was an association of actinic prurigo with HLA DR in a Colombian population. MATERIALS AND METHODS: Forty patients with a clinical diagnosis of actinic prurigo and 40 healthy subjects, paired by age, sex and birthplace, were studied. HLA typing for HLA DRB1 and DRB1*04, if necessary, was performed by the PCR-SSP method using blood samples. RESULTS: A high frequency of HLA DRB1*0407 was found in the patients (97.5% vs. 30%; P<0.00001). The allelic frequency of HLA DRB1*0407 was 63.8% in the case group, and 14.5% in the controls (P<0.00001). In the control group, there was a higher frequency of the alleles DRB1*01 (14.5% vs. 1.25%; P=0.0027) and DRB1*13 (23.7% vs. 2.5%; P=0.00013). LIMITATIONS: The small number of controls does not allow us to drive conclusions about other HLA alleles. CONCLUSIONS: HLA subtype DRB1*0407, found in actinic prurigo patients in studies conducted in England, Scotland, Ireland and Mexico, was also associated in Colombian patients. This finding, concordant in patients from different ethnic groups, could be helpful in the diagnosis of this disease and probably important in its pathogenesis. DRB1*01 and DRB1*13 alleles were more frequent in controls than in patients; we do not know whether they play any role in the resistance to the disease.     

Oxidative stress in patients with Behcet's disease: I correlation with severity and clinical parameters

The study was designed to investigate the possible correlation between some oxidative stress parameters in Behcet's disease and the clinical manifestations of the disease as well as the possible correlation with the disease severity. Seventy-six patients diagnosed according to the International Study Group criteria for Behcet's disease were included in the study. Sixty patients had mild-to-moderate disease and 16 patients had severe disease. Sixty matched control subjects were also included. After a full history and examination from each subject, 10 mL blood was drawn from each for analysis. Serum malondialdehyde, glutathione, ceruloplasmin, copper and zinc levels were determined. Patients with Behcet's disease showed increased levels of serum malondialdehyde and copper while glutathione and zinc levels were decreased. Comparison between these parameters in patients with mild-to-moderate disease with those with severe disease showed only that serum zinc levels were lower in severe Behcet's disease. Serum malondialdehyde levels were found to be significantly positively correlated with oral ulcer size, duration and frequency. Glutathione levels were found to be inversely correlated with the clinical manifestation index and all oral ulcer parameters. Zinc levels were found to be inversely correlated with the clinical manifestation index and pathergy test positivity grades. Copper levels were found to be positively correlated with oral ulcer number. Although the parameters of oxidative stress did not show correlation with disease severity, they were correlated with the disease manifestations. This points out the importance of oxidative stress in Behcet's disease.     

Is there any relationship between serum and urine neopterin and serum interferon‐γ levels in the activity of Behcet's disease?

Background  Behcet's disease (BD) is a chronic, inflammatory, multisystem vasculitic disorder. There is no reliable laboratory marker that indicates disease activity. Neopterin is an immunological marker of cellular immune activation, which is secreted by monocytes/macrophages as a result of interferon-gamma (IFN-γ) secretion by activated T lymphocytes.
Objective  We aimed to investigate serum and urine neopterin levels in BD patients.
Methods  Forty-five patients who were diagnosed according to the criteria of the International Study Group for BD and 45 age- and sex-matched healthy controls were enrolled in the study. Disease activity was considered by clinical findings. Serum and urine neopterin levels and serum IFN-γ levels were measured.
Results  The mean values of serum and urine neopterin levels were 12.68 ± 4.87 nmol/L and 167.53 ± 148.73 µmol/mol creatinine, respectively, in BD patients ( P =  0.000 and P  = 0.008, respectively), which were statistically significantly different from the control group. However, there was no significant statistical difference between serum and urine neopterin levels of the clinically active and inactive patients. It was also found that the mean value of serum IFN-γ levels was higher in healthy controls than in BD patients ( P =  0.000).
Conclusions  We conclude that serum and urinary neopterin measurement can not be used as a reliable laboratory marker as the BD patients' serum and urinary neopterin levels do not increase in the active stage even though these levels increase when compared to healthy controls.