HPLC测定睾酮乳膏中睾酮的含量

采用HPLC测定睾酮乳膏中睾酮的含量,以甲醇-水(70:30)为流动相,波长240mm,在40～360μg/ml范围内线性关系良好r==0.9999,本法操作简便,回收率高。     

中国林蛙油中睾酮的含量测定

睾酮是一种19碳甾体固醇类化合物是雄性激素中活性最强的一种。根据传统的提取工艺提取后的睾酮用放射免疫分析方法进行分析测定，其原理是通过放射性同位素标记的抗原（^125I-睾酮）和未标记抗原（待测物睾酮）与抗体的竞争作用而实现的，通过绘制标准曲线计算样品的含量。这种对睾酮的定性定量分析有利于林蛙油有效成分之一睾酮的综合开发利用。     

5α—双氢睾酮B环衍生物的合成

用改良的实验方法以３β－乙酰氧基－雄甾－５－烯－７酮为原料，经硝化、还原、水解、卤化和氧化等反应制备了５ａ－双氢睾酮Ｂ环衍生物共８个。     

高效液相色谱法测定庚酸睾酮的含量

高效液相色谱法测定庚酸睾酮的含量中国药品生物制品检定所北京１０００５０丁丽霞田敏宋育文庚酸睾酮（Ｔｅｓｔｏｓｔｅｒｏｎｅｅｎａｎｔｈａｔｅ）是激素类男用抗生育药，通过抑制精子生成而起到抗生育作用。到目前为止，临床试验已经发现，在亚洲男子中注射庚酸睾酮...     

睾酮颊黏膜吸收贴片的研制

目的制备睾酮颊黏膜贴片并考察其经颊黏膜渗透的速率。方法采用卡巴泊934、HPMC、PVA等亲水性高分子材料，将睾酮制成颊黏膜黏附贴片，以油酸、丙二醇等为促渗剂，通过对金黄地鼠颊囊进行体外μ渗透实验，考察睾酮颊黏膜贴片的体外渗透速率。结果睾酮贴片通过金黄地鼠颊黏膜的体外渗透速率为（3.12±0.84） μg·（cm2） 1·h 1，处方中加入氮酮或油酸可使渗透速率分别增大2.16和3.12倍；随着处方中油酸的用量增加，睾酮的渗透速率进一步增大。结论睾酮贴片中睾酮可经颊黏膜渗透吸收，加入促渗剂可明显增大睾酮的渗透速率。     

5α－双氢睾酮B环衍生物的合成

用改良的实验方法以３β－乙醚氧基－雄甾－５－烯－１７－酮（去氢表雄酮醋酸酯，Ⅸ）为原料，经硝化、还原、水解、卤化和氧化等反应制备了５α－双氢睾酮Ｂ环衍生物共８个。并进行了蛋白同化作用和抗辐射作用的药理实验。初步分析了结构与活性的关系．     

睾酮替代凝胶AndroGel泵上市

Unimed制药公司已经推出了其新产品1％睾酮替代凝胶AndroGel泵的计量释放“产品”。该产品被批准用于雄激素水平低下或者缺乏的男性病人的睾酮替代治疗。该公司指出这是美国最常见的睾酮处方疗法。这种“泵”最主要的好处是能让医生在批准的剂量范围内灵活选定各患者需要的药物剂量。     

促宫颈成熟剂普拉睾酮

普拉睾酮可促进宫颈成熟，缩短分娩发动时间、缩短产程和提高引产成功率，对产妇及新生儿无不良影响。该药可用于妊娠晚期、产程中、计划分娩及中期妊娠引产。     

睾酮对大鼠睾丸形态学的影响

本文研究了丙酸睾酮对大鼠睾丸形态超微结构的影响,结果发现：大剂量外源性丙酸睾酮可使睾丸出现抑制性超微结构的形态学改变。     

尿中睾酮与表睾酮的三甲基硅烷化及其比值的GC—MS测定

对睾酮及表睾酮的三甲基硅烷化进行了详细考察,找到了较好的抗氧剂巯基乙醇,确定了较好的衍生化条件,衍生化产物单一。并采用GC—MS法测定了尿中睾酮与表睾酮的比值。实验条件为:以氦为载气,SE—54熔融石英柔性毛细管柱、程序升温进行样品分离,多离子检测(MID),监测m/z432的离子。该法专属、灵敏、快速。睾酮与表睾酮比值在1:1～10:1(睾酮为20ng/μl)与相应峰面积比呈线性关系(r=0.998),最低检测限为1ng,最低检测尿药浓度为8ng/ml。     

Some solute-solvent complexes involving testosterone and testosterone propionate

Solute-solvent interactions have been studied by observing the influences of carbon tetrachloride, carbon disulphide, ethyl oleate, isopropyl myristate and octanol on the solubilities of testosterone and testosterone propionate in an inert solvent (cyclohexane). Complexation with testosterone was detected, but there was no evidence of complexation between testosterone propionate and these solvents. The solvent-induced infrared shifts were due to non-specific solvent effects. The existence of solute-solvent complexes between the two solutes and chloroform, previously detected by infrared absorption, was confirmed. The solubility technique, which has previously only been used with solid complexing agents, is adaptable to liquid complexing agents, but has limitations.     

Short-term pharmacokinetic comparison of a novel testosterone buccal system and a testosterone gel in testosterone deficient men

SUMMARY

Objective: The primary objective of the study was to compare the percentage of men with mean serum total T (Cave(0–24)) within normal range during the 24-h pharmacokinetic (PK) sampling period on Days 14 and 15.

Methods: Treatment with a new testosterone (T) buccal system, (Striant), 30mg twice daily was compared to a transdermal gel delivery system, (T-gel) [AndroGel 5?g containing 1% (50?mg) T] daily for 14days in T-deficient men. Safety parameters included laboratory assessments and collection of adverse events. Patients were otherwise healthy T-deficient men with total T?<?8.7 nmol/L (< 2.5?ng/mL).

Results: Twenty-six of the 28 patients enrolled completed the 24-h PK assessment. Of the evaluable patients, 92.3% of T buccal system and 83.3% of T-gel patients had Cave(0–24) within the normal range of 10.4-36.4 nmol/L (3.0–10.5?ng/mL). Mean total T values were not different in the T buccal system group (Cave(0–24) 16.7?±?4.7?nmol/L; 4.8?±?1.4?ng/mL) compared to the T-gel group (Cave(0–24) 15.9?±?4.8?nmol/L; 4.6?±?1.4?ng/mL). All T values returned to baseline levels after the study drug was stopped. Serum LH and FSH levels decreased, and E2 increased as expected following T administration. Differences in DHT concentrations between treatment groups were significant (p?=?0.012) with mean DHT levels on Day 14 of 1.9?±?1.4 nmol/L (0.55?±?0.42?ng/mL) for the T buccal system and 3.2?±?1.3?nmol/L (0.93?±?0.38ng/mL) for T-gel, which was greater than the upper level of normal (2.9?nmol/L; 0.85?ng/mL). Statistically significant differences were seen in the mean T/DHT ratio on Days 14     

Short-term pharmacokinetic comparison of a novel testosterone buccal system and a testosterone gel in testosterone deficient men

OBJECTIVE: The primary objective of the study was to compare the percentage of men with mean serum total T (C(ave(0-24))) within normal range during the 24-h pharmacokinetic (PK) sampling period on Days 14 and 15. METHODS: Treatment with a new testosterone (T) buccal system, (Striant), 30 mg twice daily was compared to a transdermal gel delivery system, (T-gel) [AndroGel 5 g containing 1% (50 mg) T] daily for 14 days in T-deficient men. Safety parameters included laboratory assessments and collection of adverse events. Patients were otherwise healthy T-deficient men with total T 相似文献   

Identification of testosterone and testosterone esters in human hair.

In 1974, steroids were added to the list of doping agents banned by the International Olympic Committee because of their effects on the performance of the athletes. Testosterone and its esters promote the development of secondary male sexual characteristics and accelerate muscle growth. The mandatory test to detect testosterone abuse is to measure the ratio of testosterone to epitestosterone in the urine. However, because athletes can adjust their dosage to stay within the range permitted, there is a risk of test evasion. Therefore, we developed two original procedures to determine testosterone and its esters in human hair. First, testosterone was investigated in hair obtained from 26 control subjects. After decontamination with dichloromethane, 100 mg of hair was incubated in 1 M NaOH in the presence of 1 ng of testosterone-d3. After neutralization, the extract was purified using solid-phase extraction with Isolute C18 columns followed by liquid-liquid extraction with pentane. After silylation, testosterone was analyzed by gas chromatography-mass spectrometry. Concentrations were in the range 1.2 to 11.4 pg/mg with a mean value of 3.8 pg/mg. To distinguish exogenous abuse from endogenous levels, the incorporation of testosterone esters into hair was investigated. Preparation involved methanolic incubation to avoid the cleavage of the esters. In a panel of eight esters, it was possible to identify testosterone propionate, testosterone enanthate, and testosterone decanoate in the hair of two bodybuilders and one weight lifter. This new technology may find useful applications in anabolic abuse control.     

Absolute bioavailability of testosterone after oral administration of testosterone-undecanoate and testosterone

The plasma levels of testosterone (T) were measured after oral administration of 25 mg T and 40 mg testosterone-undecanoate (TU) in a group of young women by a specific radioimmunoassay. Plasma levels were compared to those after intravenous administration of 1.5 micrograms testosterone/kg to another group of young women for determination of absolute bioavailability. Due to the high metabolic clearance rate of 24.5 ml/min/kg absolute systemic availability of free testosterone was calculated to 3.56 +/- 2.45%. Oral administration of testosterone undecanoate leads only to an absolute testosterone bioavailability of 6.83 +/- 3.32%.     

高效液相色谱法测定复方丙睾凝胶剂中丙酸睾酮的含量

目的:测定复方丙睾凝胶剂中丙酸睾酮的含量。方法:用高效液相色谱法,采用Kromasil C18柱,以甲醇-水(85∶15)为流动相,流速为0.8 ml/min,检测波长为254 nm,柱温为25℃。结果:丙酸睾酮在0.030 3~0.161 6 mg/ml内呈现良好的线性关系(r=0.999 9)。结论:本法可灵敏、快速、准确测定复方丙睾凝胶剂中丙酸睾酮的含量。