子痫前期患者血清瘦素及可溶性瘦素受体水平检测分析

目的探讨血清瘦素(leptin,Lep)和可溶性瘦素受体(solub le leptin receptor,sLR)水平与子痫前期的关系。方法应用放射免疫分析法(R IA)及酶联免疫吸附分析法(ELISA)检测子痫前期40例(子痫前期组:其中轻度子痫前期20例,重度子痫前期20例)和正常妊娠妇女20例(对照组)血清Lep和sLR的水平。结果①子痫前期血清Lep水平(25.07±6.34)较对照组(19.07±5.80)高,差异有统计学意义(t=3.665,P<0.01);sLR水平(22.58±3.64)较对照组(31.22±4.32)低,差异亦有统计学意义(t=4.321,P<0.01)。②轻、重子痫前期组之间相比较,Lep水平前者较后者低,差异有统计学意义(P<0.01)。sLR前者较后者高,差异亦有统计学意义(P<0.01)。③Lep和sLR表达水平呈负相关(r=-0.367,P<0.05)。结论子痫前期的发病可能与Lep及sLR异常有关。     

血清瘦素及可溶性瘦素受体水平与2型糖尿病的关系

目的探讨血清瘦素和可溶性瘦素受体水平与2型糖尿病的关系。方法应用放射免疫分析法(RIA)及酶联免疫吸附分析法(ELISA)检测56例2型糖尿病患者及38例健康对照者血清瘦素(Lp)、可溶性瘦素受体(sLR),同时检测总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白ApoA、ApoB、ApoA/B、体质量指数(BMI)、腰围、臀围等指标,分析Lp、sLR与2型糖尿病、血脂异常、体形改变、BMI的关系。结果2型糖尿病患者血清Lp、TC、LDL-C、ApoB明显高于健康对照者(P<0.05),sLR、ApoA/B明显低于健康对照者(P<0.01),TG、HDL-C、ApoA及BMI无显著性差异。Lp与BMI、腰围及臀围呈正相关,与sLR呈负相关(P<0.05);sLR与Lp、BMI、腰围及臀围呈负相关(P<0.05)。结论2型糖尿病与Lp及sLR异常有关,其与血脂异常、体形改变、BMI密切相关,LP及sLR共同参与2型糖尿病的发生发展。     

超敏C反应蛋白、白细胞介素-6、瘦素与2型糖尿病肾病的相关性研究

目的探讨在糖尿病肾病的发生过程中血超敏C反应蛋白（usCRP）、白细胞介素-6（IL-6）、瘦素（LEP）的变化。方法根据尿白蛋白排泄率（UAER）将123例2型糖尿病患者分为正常白蛋白尿组、微量白蛋白尿组、临床白蛋白尿组,另选31例作为正常对照组,观察各组血中usCRP、IL-6、LEP水平并进行比较,与有关指标进行相关性分析。结果2型糖尿病各组血中usCRP、IL-6、LEP水平较正常对照组明显升高,且usCRP、IL-6、LEP水平随白蛋白尿的增加而升高。结论血usCRP和IL-6、LEP浓度增高可能是糖尿病肾病的重要危险因素;炎症反应强度与肾病的严重程度有关。     

肝硬化患者血清中瘦素及可溶性瘦素受体的临床研究

目的 观察不同程度肝硬化患者血清中瘦素(leptin,LP)及可溶性瘦素受体(soluble leptin receptor,SLR)的水平,探讨LP及SLR在肝硬化发生发展中所起的作用及相关机制,揭示血清LP及SLR是否可作为临床评估患者肝硬化程度的非创伤性检测指标.方法 清晨空腹抽血5ml,分离血清后放在-20℃冰箱中备检.用EUSA法检测血清中LP及SLR水平.结果 肝硬化各级患者LP水平均较对照组明显升高,且各级间差异亦有极显著性(P<0.01),随着肝硬化严重程度进展,LP水平相对增加,而SLR较对照组明显降低,各级间差异亦有显著性(均P<0.01).结论 血清中LP及SLR测定可作为反映肝实质损伤程度的敏感指标,对肝硬化程度的判定具有重要意义.     

阿尔茨海默病患者血清超敏C反应蛋白、白细胞介素-6及丙二醛水平的变化

目的研究阿尔茨海默病(AD)患者较同期体检健康老年人血清超敏C反应蛋白(HS-CRP)、白细胞-6(IL-6)及丙二醛(MDA)水平是否存在差别,借以分析氧化应激和炎性反应是否参与AD的发病。方法分别抽取60例AD患者和60名同期门诊体检健康老年人的空腹静脉血,测定其血清HS-CRP、IL-6、MDA水平,并予以比较分析。结果 AD患者血清HS-CRP、IL-6、MDA含量均较同期门诊体检健康老年人明显升高,差异经检验,具有统计学意义(P<0.01)。结论氧化应激和炎性反应可能参与了AD的发病。     

瘦素、瘦素受体水平及基因多态性与川崎病的关联研究

目的探讨瘦素(LEP)及瘦素受体(LEPR)水平在川崎病(KD)患儿中的变化及临床意义,分析LEP基因rs2167270、LEPR基因rs1137100多态性与川崎病易感性之间的关系。方法选取53例汉族川崎病患儿为实验组,年龄6个月～5岁;选取同期行健康体检的53例汉族儿童为对照组。运用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)分析各组LEP基因rs2167270、LEPR基因rs1137100多态性。结果实验组中发热53例(100.0%),皮疹40例(75.5%),非化脓性眼结合膜充血48例(90.6%),口腔黏膜充血29例(54.7%),非化脓性淋巴结肿大46例(86.8%),手足硬肿32例(60.4%),指趾端脱皮20例(37.7%),肛周脱皮14例(26.4%),冠状动脉扩张4例(7.5%)。实验组LEP水平为(0.290±0.055)ng/ml,高于对照组的(0.209±0.039)ng/ml,差异有统计学意义(P<0.05)。实验组LEPR水平为(10.951±2.530)ng/ml,高于对照组的(7.238±1.780)ng/ml,差异有统计学意义...     

肥胖儿童瘦素及可溶性受体与胰岛素关系

目的 分析肥胖儿童血清中瘦素、可溶性瘦素受体、胰岛素间相互关系.方法 以儿童BMI肥胖筛选标准筛选出12～14岁肥胖儿童99例,按1∶1配对原则选择体重正常的同年龄、同性别、身高相近(-x±s以内)儿童作为对照,用放射免疫法测定其血清瘦素、胰岛素及C-肽含量,用酶联免疫吸附分析法测定可溶性瘦素受体含量.结果 肥胖儿童血清瘦素、胰岛素水平(28.72±15.13、36.21±15.37)明显高于正常组(7.65±4.84、20.46±13.72);肥胖组儿童可溶性瘦素受体水平(12.81±4.20)明显低于正常组(17.28±6.45);两组儿童LEP与sOB-R皆呈负相关,相关系数分别为-0.418和-0.312;两组儿童LEP与INS、C-肽皆呈正相关,相关系数分别为0.380、0.540和0.284、0.328;两组儿童FLI与胰岛素和C-肽之间相关系数分别为0.315、0.408和0.379、0.381.结论 肥胖儿童体内瘦素、胰岛素水平均高于正常组,肥胖儿童体内存在着瘦素抵抗和胰岛素抵抗现象;瘦素可通过影响胰岛素的分泌来调节外周血中的胰岛素含量.     

婴幼儿血清可溶性白细胞介素-2受体测定

分析101例健康婴幼儿血清sIL-2R检测结果,资料显示婴幼儿阶段血清中sIL-2R的含量明显高于正常成人。婴幼儿不同阶段sIL-2R均值变化特点同年龄分布有一定相关性,其特点是新生儿明显增高,2岁时达到高峰,之后呈逐年递减趋势。表明新生儿、婴儿阶段机体细胞免疫水平发展迅速,5岁以后逐渐平缓并向正常水平过渡。揭示健康婴幼儿血清sIL-2R水平的变化特点,为了解婴幼儿的免疫功能状态以及推测各种疾病对婴幼儿免疫功能的影响提供了重要的实验方法,并为国人提供了有价值的实验参考数据。     

胆囊胆固醇沉积症患者血清瘦素、瘦素受体与血脂的相关性研究

目的 探讨胆囊胆固醇沉积症患者血清瘦素(Leptin,Lep)、可溶性瘦素受体(soluble leptin receptor,sLR)及血脂的变化及其相互关系.方法 胆囊胆固醇沉积症患者49例,对照组91例,测定其空腹血清瘦素、可溶性瘦素受体、胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL),分析胆囊胆固醇沉积症患者血清Lep、sLR水平与血脂的关系.结果 胆囊胆固醇沉积症组患者血清TC、LDL和Lep值均显著高于对照组,sLR显著低于对照组,差异有统计学意义(P<0.05),单相关分析显示Lep与TC、TG呈正相关.结论 胆囊胆固醇沉积症患者血清瘦素水平与血脂异常有相关性.     

原发性高血压患者血清IL-6和C-反应蛋白水平的研究

目的：探讨在原发性高血压患者中,血清C-反应蛋白（CRP）和白细胞介素-6（IL-6）水平变化及意义。方法：选择42例正常血压人群和36例原发性高血压患者,常规测量血压,抽取空腹静脉血检测其血糖、血脂、胰岛素、IL-6、CRP。结果：与正常人群相比,在高血压患者中,血清总胆固醇、三酰甘油、空腹胰岛素、IL-6水平（14.56±5.36vs23.16±2.43μg/ml,P〈0.05）、CRP（1.52±1.04vs2.05±1.47mg/ml,P〈0.05）水平均高于正常血压人群。在原发性高血压患者中,血清IL-6、CRP水平均与体重指数、收缩压正相关。结论：原发性高血压患者中血清IL-6、CRP水平增高并与收缩压正相关,提示原发性高血压与炎症密切相关。     

A cholecystokinin-1 receptor agonist (CCK-8) mediates increased permeability of brain barriers to leptin

BACKGROUND AND PURPOSE: Leptin regulates energy expenditure and body weight by acting both on the hypothalamus and on peripheral targets. Central actions of leptin are enhanced by cholecystokinin (CCK). The interaction between leptin and CCK makes physiological sense, as rats lacking CCK1 receptors are resistant to peripheral leptin but not to leptin directly infused into the brain. We have recently reported that CCK enhances leptin effects by increasing the entry of leptin into the CNS. The aim of this work was to further characterize the effect of CCK (10 microg kg(-1)) on leptin kinetics as well as the CCK receptor subtype involved in the interaction between CCK and leptin. EXPERIMENTAL APPROACH: Experiments were carried out both in free-feeding and in fasted rats receiving a single dose of leptin (100 microg kg(-1); i.p.). Parameters analysed over the next 6 h were plasma and cerebrospinal fluid concentrations of leptin. KEY RESULTS: We observed that CCK-8 depressed the increase in plasma leptin that followed the i.p. injection and simultaneously increased leptin concentration in the cerebrospinal fluid from 92+/-25 to 230+/-24 pg mL(-1) (P<0.05). The effect of CCK-8 was totally prevented by the CCK1 receptor antagonist, SR-27,897 (0.3 mg kg(-1), s.c.), but not by the CCK2 receptor antagonist, L-365,260 (1 mg kg(-1)). CONCLUSIONS AND IMPLICATIONS: These results show that CCK plays a role in regulating the access of leptin to the brain and suggest that CCK analogues, acting on CCK1 receptors, might be useful drugs in improving leptin actions within the brain.     

24 h 尿蛋白、胱抑素C、D-二聚体及超敏C-反应蛋白与妊娠期高血压疾病严重程度的相关性及对不良妊娠结局的预测价值

目的探讨24 h尿蛋白(24 h PRO)、胱抑素C(CysC)、D-二聚体(D-D)及超敏C-反应蛋白(hs-CRP)与妊娠期高血压疾病(HDP)严重程度的相关性及对不良妊娠结局的预测价值。方法选取2017年1月至2019年12月淮南市第一人民医院收治的190例HDP孕妇(其中妊娠期高血压组29例,子痫前期组96例,重度子痫前期组65例)作为HDP组,同时选取190例在淮南市第一人民医院正常产检并产生妊娠结局的孕妇作为对照组。检测两组24 h PRO、CysC、D-D、hs-CRP、血肌酐(Scr)、纤维蛋白原(FIB)、凝血酶原时间(PT)、活化部分凝血酶原时间(APTT)、凝血酶时间(TT)以及尿素氮(BUN)水平,评估其与HDP严重程度的相关性,并建立预测模型评估其对不良妊娠结局的预测价值。结果HDP 组病人的24 h PRO［(1.03±0.80)g/24 h比(0.14±0.11)g/24 h］、CysC［(1.47±0.41)mg/L比(1.23±0.28)mg/L］、D-D［(760.12±275.43)μg/L比(530.98±109.20)μg/L］、hs-CRP［(7.08±3.56)mg/L比(4.01±2.02)mg/L］、Scr［(70.98±9.65)μmol/L比(69.12±8.23)μmol/L］及FIB［(4.40±0.77)g/L比(4.19±0.53)g/L］水平均高于对照组(P<0.05),与疾病的严重程度呈正相关(rs =0.76,0.30、0.33、0.30、0.21、0.19,P<0.05);PT［(10.05±1.09)s比(10.42±0.97)s］、APTT［(27.62±2.67)s比(28.19±2.17)s］及TT［(13.78±1.23)s比(14.08±1.26)s］水平低于对照组(P<0.05),与病人疾病的严重程度呈负相关(rs =?0.22、?0.16、?0.17,P<0.05)。24 h PRO、CysC、D-D、hs-CRP、SCr、PT在HDP中不良妊娠结局组与正常妊娠结局组均差异有统计学意义(P<0.05)。多因素二元logistic回归分析显示24 h PRO、CysC、D-D及hs-CRP是HDP病人不良妊娠结局的独立影响因素(OR=1.98、5.38、1.00和1.28,P<0.05)。ROC曲线显示24 h PRO、CysC、D-D、hs-CRP及四项指标联合预测不良妊娠结局的曲线下面积(AUC)为72.0%、70.6%、74.6%、75.7%、86.7%。结论HDP病人24 h PRO、CysC、DD及hs-CRP水平同病情严重程度呈正相关,联合评估对于预测不良妊娠结局有一定的应用价值,临床工作中应密切监护上述指标的动态变化,评估病情进展。     

Circulating levels of advanced glycation end products (AGE) and interleukin-6 (IL-6) are independent determinants of serum asymmetric dimethylarginine (ADMA) levels in patients with septic shock

There is a growing body of evidence that nitric oxide (NO) excess plays a central role in the pathogenesis of hypotension and organ failure in patients with septic shock. In addition, recently, asymmetric dimethylarginine (ADMA), an endogenous inhibitor of NO synthase, has been shown to contribute to the regulation of vascular tone via modulation of NO generation in vivo. However, the kinetics and regulation of serum levels of ADMA in patients with septic shock are largely unknown. Since high mobility group box 1 (HMGB1)-receptor for advanced end products (RAGE) axis is supposed to be involved in the lethality in septic shock, we examined the correlations among serum levels of ADMA, endotoxin, interleukin-6 (IL-6), soluble form of RAGE (sRAGE) and RAGE ligands such as HMGB1 and advanced glycation end products (AGE) in septic shock patients. Fifteen septic shock patients (10 males and 15 females, mean age: 70.1 ± 8.5 years) and fifteen age- and sex-matched healthy volunteers were included in this study. The criteria of the American College of Chest Physicians/Society of Critical Care Medicine Consensus Conference were used for diagnosis of septic shock. All the subjects underwent a complete history and physical examination, determination of blood chemistries, including serum levels of ADMA, endotoxin, IL-6, HMGB1, AGE and sRAGE. Linear and multiple stepwise regression analysis were performed for the determinants of serum levels of ADMA. Serum levels of ADMA were significantly higher than those in healthy volunteers (0.98 ± 0.21 nmol/mL vs. 0.30 ± 0.05 nmol/mL, p < 0.0001). In univariate analysis, creatinine (p < 0.005), endotoxin (p < 0.001), IL-6 (p < 0.001), HMGB1 (p < 0.001), AGE (p < 0.001) and sRAGE (p < 0.001) were significantly associated with serum ADMA levels. After performing multivariate stepwise regression analyses, IL-6 (p = 0.001), AGE (p = 0.002) and creatinine (p = 0.013) still remained significant independently. The present study is the first demonstration that ADMA levels were significantly elevated in patients with septic shock and that serum IL-6, AGE and creatinine levels were independent determinants of ADMA in these patients. Given the harmful effects of NO excess in septic shock, ADMA levels may be increased as a counter-system against inflammation and oxidative stress in this life-threatening disorder.     

Serum levels of IL-6 and its soluble receptor, TNF-alpha and chemokine RANTES in scorpion envenomed children: their relation to scorpion envenomation outcome.

During the present study, 30 children in Upper Egypt (less than 12 years old) were admitted to Pediatric Intensive Care Unit because of scorpion envenomation. They were compared with 20 apparently normal children of matching age and sex as controls. The victims and controls were subjected to complete clinical examination and full blood picture. The serum levels of interleukin-6 (IL-6), soluble IL-6 receptor (sIL-6R), regulated upon activation normal T cells expressed and secreted (RANTES ) and tumour necrosis factor-alpha (TNF-alpha) were determined once for the controls and twice for the victims, the first sample on admission and the second sample after 24h. All victims showed significantly higher mean values of IL-6, sIL-6R, RANTES, TNF-alpha, and leucocytic count both on admission and on the follow up when compared with controls. According to the clinical manifestations of envenomation, 40% of the victims had a mild envenomation manifestation, while 60% of them had severe manifestations. The severely envenomed children showed significantly higher mean values of IL-6, sIL-6R, TNF-alpha, RANTES and leucocytic count both on admission and on the follow up samples when compared with the mild cases. The non-survival victims (five victims) showed significantly higher mean values of IL-6, sIL-6R, TNF-alpha, RANTES and leucocytic count both on admission and on the follow up samples in comparison to the survivals. Furthermore, those fatal cases showed a non-significant decline in the serum levels of IL-6, sIL-6R, TNF-alpha, RANTES and leucocytic count on the following up samples, while the survivals showed a significant decline in the serum levels of these parameters on the following up samples. In conclusion, these data revealed that IL-6, sIL-6R, TNF-alpha and chemokine, RANTES are involved in the pathogenesis of scorpion envenomation and correlated with its severity.     

Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression

BackgroundDepression can be perceived as a psychoneuroimmunological disorder in which cytokines affecting the body's neurochemical and neuroendocrine functions play an important role. Among cytokines, chemokines participating in activation of the inflammatory response are considered to be crucial.Methods160 men and women were enrolled in the study. 120 of them were diagnosed with various types of depression. The mean age was 45.2 ± 4.5 years (range: 19–47 years). The control group consisted of 40 healthy individuals. The average age in this group was 42.4 ± 4.1 years. Plasma levels of chemokines and their receptors (CCL-5 – RANTES and CXCR-5, SDF-1 and CXCR-4), as well as of IL-6, were assessed by ELISA.ResultsThere was an increase in SDF-1 and CCL-5 levels in women and men with different severities of depression, versus the control group. Also, an increase in the IL-6 levels, CXCR4 and CCR-5 receptors was observed in both women and men with all types of depression. Levels of SDF-1 and CCL-5 chemokines, as well as of CCR-5 and CXCR4 chemokine receptors, were higher in women than in men.ConclusionsThe results of this study indicate the need for assessment of CCL-5 and SDF-1 chemokines levels, as they are likely markers of developing depression. Early measurement of these chemokines levels may be helpful in choosing the best pharmacotherapy.     

Synergism between interleukin (IL)-17 and toll-like receptor 2 and 4 signals to induce IL-8 expression in cystic fibrosis airway epithelial cells

Cystic fibrosis (CF) is the most common lethal inherited disorder and is caused by mutations in the gene encoding the CF transmembrane regulator (CFTR). The CF lung expresses a profound proinflammatory phenotype that appears to be related to a constitutive hypersecretion of interleukin (IL)-8 from airway epithelial cells in response to microbial infection. Since overproduction of IL-8 in CF contributes to massive bronchial infiltrates of neutrophils, identification of the pathways underlying IL-8 induction could provide novel drug targets for treatment of neutrophil-dominated inflammatory diseases such as CF. Here, we show that IL-17A synergistically increases IL-8 production induced by a toll-like receptor (TLR) 2 agonist, peptidoglycan (PGN), or TLR4 agonist, lipopolysaccharide (LPS), in a human CF bronchial epithelial cell line (CFBE41o-). A strong synergism was also observed in primary human CF bronchial epithelial cells, but not in human non-CF cell lines and primary cells. Notably, despite the induction of nuclear factor-κB and MAP kinases during TLR2 or TLR4 activation in CFBE41o-, IL-17A-dependent synergism appears to be the result of enhanced PGN- or LPS-induced phosphorylation of p38. Taken together, these studies provide evidence that IL-17A is a critical factor in increasing IL-8 expression in bacteria-infected CF airways via a pathway that regulates p38 phosphorylation.     

Long-term administration of m-chlorophenylpiperazine (mCPP) to rats induces changes in serotonin receptor binding,dopamine levels and locomotor activity without altering prolactin and corticosterone secretion

Meta-chlorophenylpiperazine (mCPP) is a serotonin (5-HT) agonist with antidepressant actions. In order to investigate the effects of chronic mCPP treatment the drug was administered to rats for 15 days (5 mg/kg twice daily). Controls were administered saline. Long-term mCPP treatment led to a 36% increase in [³H]8-hydroxy-2-(di-n-propylamino)tetralin ([³H]8-OH-DPAT) binding to 5-HT_1a receptors in hippocampus and a 74% decrease in [³H]ketanserin binding to 5-HT₂ receptors in cortex, while (-)[¹²⁵I]iodocyanopindolol ([¹²⁵I]CYP) binding to 5-HT_1b receptors in hypothalamus and striatum was unchanged. In hypothalamus, chronic mCPP treatment decreased the levels of dopamine (DA) but not 5-HT. The usual suppression of locomotor activity induced by acute mCPP administration was less after long-term mCPP treatment. Brain and plasma levels of mCPP following an acute dose were not different between controls and rats previously administered mCPP, suggesting that altered rate of metabolism of the drug did not explain the tolerance to the mCPP-induced decrease in locomotor activity. mCPP-induced prolactin (PRL) and corticosterone release were not changed by previous long-term mCPP administration. Thus, chronic mCPP administration to rats induced alterations in density of 5-HT receptor subtypes, hypothalamic levels of DA and locomotor behavior.