艾灸对类风湿性关节炎大鼠关节滑膜细胞超微结构的影响

目的:观察艾灸"肾俞""足三里"穴对类风湿性关节炎模型大鼠关节滑膜细胞的影响,为艾灸抗炎的免疫作用机制提供新的依据.方法:120只雄性Wistar大鼠随机选取20只为正常组,其余大鼠采用风、寒、湿环境因素结合弗氏完全佐剂的方法复制类风湿性关节炎模型,而后随机分为模型组、艾灸组、针刺组、药物组、CO2激光组,每组20只....     

不同产地宽叶荨麻对人类风湿性关节炎滑膜成纤维细胞增殖和凋亡的影响

目的 研究不同产地宽叶荨麻对人类风湿性关节炎滑膜成纤维细胞MH7A增殖和凋亡的影响.方法 分别以庄河、鲁甸、阿坝、巴株产宽叶荨麻70％乙醇提取物处理MH7A细胞,24h后用MTT法检测细胞增殖率.通过测定DNA片段化检测细胞凋亡率.结果 4种产地的宽叶荨麻都显示了一定的抑制MH7A细胞增殖的作用.其中鲁甸产宽叶荨麻显示了较强的抑制作用,因此进一步考察了鲁甸产宽叶荨麻不同组分对MH7A细胞增殖的影响,其中经大孔吸附树脂吸附以80％ EtOH-H2O洗脱部分(LD80)显示了较强的抑制MH7A细胞增殖的作用.DNA片段化分析结果表明,LD80能显著引起MH7A细胞凋亡.结论 鲁甸产宽叶荨麻能够抑制MH7A细胞增殖和诱导MH7A细胞凋亡.     

Analgesic effect of different moxibustion durations in rheumatoid arthritis rats

OBJECTIVE： To observe the influence of different moxibustion durations on hypothalamic pro-opi- omelanocortin（POMC） and prodynorphin（PDYN） mRNA expressions and plasma β-endorphin（β-EP） content in rheumatoid arthritis（RA） rats, to under- stand the mechanism of moxibustion analgesia and its dose-effect relationship. METHODS： Twelve male Wistar rats were randomly selected from 48 male Wistar rats as a normal con- trol group. The RA model was created by raising rats in a windy（blowing with electric fan）, cold（6 ℃ ±2 ℃）, and wet（80%-90% humidity） environ- ment for 20 days, 12 h each day. This was followed byinjectionofFreund＇scompleteadjuvant（0.15 mL） into the ankle. Then, rats were randomly divided in- to a model group, moxibustion group Ⅰ, and moxi- bustion group Ⅱ, with 12 rats in each group. In moxibustion groups Ⅰ and Ⅱ, moxibustion was given at Shenshu（BL 23） and Zusanli（ST 36） for 20 and 40 min, respectively, once daily for 15 days. Hy- pothalamic POMC and PDYN mRNA expression lev- els and plasma β-EP content were determined.RESULTS： Compared with the normal group, the pressure pain threshold decreased, while the hypo- thalamic POMC and PDYN mRNA expression levels and plasma β-EP content increased in the moxibus- tion groups（P0.01）. Compared with the model group, the pressure pain threshold, hypothalamic POMC and PDYN mRNA expression levels and plas- ma β-EP content in the moxibustion groups in- creased significantly（P0.01）. Compared the moxi- bustion groupⅠ, the pain threshold, hypothalamic POMC and PDYN mRNA expression levels and plas- ma β-EP content in moxibustion groupⅡsignifi- cantly increased（P0.01）. CONCLUSION： Moxibustion has an analgesic effect and increases hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content inRArats.Theanalgesiceffectinmoxibustiongroup ⅡisbetterthanthatinmoxibustiongroupⅠ.     

Analgesic effect of different moxibustion durations in rheumatoid arthritis rats

Objective

To observe the influence of different moxibustion durations on hypothalamic pro-opiomelanocortin (POMC) and prodynorphin (PDYN) mRNA expressions and plasma β-endorphin (β-EP) content in rheumatoid arthritis (RA) rats, to understand the mechanism of moxibustion analgesia and its dose-effect relationship.

Methods

Twelve male Wistar rats were randomly selected from 48 male Wistar rats as a normal control group. The RA model was created by raising rats in a windy (blowing with electric fan), cold (6°C ± 2°C), and wet (80%-90% humidity) environment for 20 days, 12 h each day. This was followed by injection of Freund's complete adjuvant (0.15 mL) into the ankle. Then, rats were randomly divided into a model group, moxibustion group I, and moxibustion group II, with 12 rats in each group. In moxibustion groups I and II, moxibustion was given at Shenshu (BL 23) and Zusanli (ST 36) for 20 and 40 min, respectively, once daily for 15 days. Hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content were determined.

Results

Compared with the normal group, the pressure pain threshold decreased, while the hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content increased in the moxibustion groups (P<0.01). Compared with the model group, the pressure pain threshold, hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content in the moxibustion groups increased significantly (P<0.01). Compared the moxibustion group I, the pain threshold, hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content in moxibustion group II significantly increased (P<0.01).

Conclusion

Moxibustion has an analgesic effect and increases hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content in RA rats. The analgesic effect in moxibustion group II is better than that in moxibustion group I.     

隔物温和灸对完全弗氏佐剂关节炎大鼠滑膜病变的影响

目的：探讨隔物温和灸的疗效及作用机制。方法：以AA模型大鼠作为实验动物,组织病理学入手,观察隔物温和灸对滑膜病变的影响。结果：各个治疗组均可以均能有效减轻AA大鼠滑膜炎症,抑制滑膜细胞增殖。结论：隔物温和灸能有效地改善AA大鼠关节炎原发病变,达到与MTX相似的效果,且与MTX联合应用有协同作用,其作用机制可能与抑制关节滑膜细胞的增生有关。     

Experimental study on the effect of different moxibustion durations on rats with rheumatoid arthritis

Objective

To observe the effect of different moxibustion durations on rats with rheumatoid arthritis (RA) and to evaluate the relationship between moxibustion amount and moxibustion efficacy.

Methods

Eight rats were randomly selected as a normal group from the 40 male Sprague-Dawley (SD) rats, and the other 32 rats were used to establish type II collagen-induced RA models. After successful modeling, the 32 rats were randomly divided into a model group, a moxibustion for 20 min group, a moxibustion for 40 min group and a moxibustion for 60 min group, with 8 rats in each group. Rats in the normal group did not receive modeling and moxibustion intervention; rats in the model group did not receive moxibustion after modeling; rats in the moxibustion for 20 min group, the moxibustion for 40 min group and the moxibustion for 60 min group were treated with moxibustion at Shenshu (BL 23) and Zusanli (ST 36) for 20 min, 40 min and 60 min, respectively. Six days were a course of treatment, with a total of 3-course treatments and a 1-day rest between the courses of treatment. After treatment, the serum levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α, arthritis index (AI) scores, toe volumes and pathological score of synovitis were evaluated in the rats.

Results

Compared with the normal group, the serum IL-1β and TNF-α levels, and the toe volumes in the model group were increased, and the differences were statistically significant (P<0.01), before the treatment. Compared with the model group, the serum IL-1β and TNF-α levels, toe volumes and arthritis index (AI) scores were significantly decreased in the moxibustion for 20 min group, the moxibustion for 40 min group and the moxibustion for 60 min group (P<0.05 or P<0.01 ). Compared with the moxibustion for 20 min group and the moxibustion for 60 min group, serum IL-1β and TNF-α levels, toe volumes and AI scores were decreased more significantly in moxibustion for 40 min group, and the differences were statistically significant (P<0.05 or P<0.01). There were no significant differences in serum IL-1β and TNF-α levels, AI scores and toe volumes between the moxibustion for 20 min group and the moxibustion for 60 min group (all P>0.05). The synovial histopathological improvement was the most obvious in the moxibustion for 40 min group, when the synovial histopathological changes were compared among the moxibustion for 20 min group, moxibustion for 40 min group and moxibustion for 60 min group.

Conclusion

The therapeutic efficacy of moxibustion for 40 min in RA rats was more significant than that of moxibustion for 20 min and moxibustion for 60 min, indicating that the duration of moxibustion is the main factor affecting its therapeutic efficacy.

     

Effect of moxibustion on autophagy and the inflammatory response of synovial cells in rheumatoid arthritis model rats

OBJECTIVE: To investigate the effect of moxibustion on synovitis and the autophagy of synoviocytes in rheumatoid arthritis(RA). METHODS: Forty Sprague-Dawley rats were randomly divided into a normal group, model group, moxibustion group, cigarette moxibustion group, and medicine group, with eight rats included in each group. The RA model was established by subcutaneous injection of complete Freund’s adjuvant into the left posterior toe. Rats in the model group were not interfered with. In the mo...     

艾灸对类风湿关节炎大鼠下丘脑POMC mRNA 和PDYN mRNA表达的影响

目的:探讨艾灸镇痛作用的中枢机制.方法:将雄性Wistar大鼠在实验前进行压痛阈值筛选,将压痛阈在(250±25)g之间的48只保留.从中随机选取12只为正常组,其余大鼠采用风、寒、湿环境因素结合弗氏完全佐剂的方法复制类风湿性关节炎模型,然后再随机分成模型组、艾灸组、艾油组,每组12只.后两组选用“肾俞”和“足三里”穴,以艾灸和涂抹艾油为治疗手段,疗程15 d;模型组、正常组不予治疗干预.观察大鼠患足压痛阈值的改变和下丘脑前阿黑皮素(POMC) mRNA和前强啡肽原(PDYN) mRNA的表达量.结果:与正常组比较,模型组大鼠压痛阈值、下丘脑POMC mRNA和PDYN mRNA的表达量升高(均P＜0.01);与模型组比较,艾灸组大鼠压痛阈值、下丘脑POMC mRNA和PDYN mRNA表达量进一步升高(均P＜0.01),而艾油组的变化无统计学意义(P＞0.05);与艾油组比较,艾灸组大鼠压痛阈值、下丘脑POMCmRNA和PDYN mRNA表达量均升高(均P＜0.01).结论:艾灸具有镇痛作用,其机制可能与艾灸的温热作用提高大鼠下丘脑POMC mRNA和PDYN mRNA的表达量有关.     

胡桃醌对类风湿关节炎关节成纤维样滑膜细胞增殖和凋亡的影响

目的探讨胡桃醌对类风湿关节炎关节成纤维样滑膜细胞(RA-FLSs)增殖和凋亡的影响。方法不同浓度胡桃醌(0、5、10、20、30、40、50μmol/L)孵育RA-FLSs 24 h,胡桃醌(30μmol/L)分别和不同终质量浓度Akt激动剂SC79(0、0.1、0.5、0.8、1.0、1.5、1.8μg/mL)同时孵育RA-FLSs 24 h。BrdU和MTT法分别检测RA-FLSs的增殖能力和细胞活力,Annexin-V FITC/PI法和caspase-3活性检测试剂盒检测RA-FLSs凋亡,Western blot法检测Akt磷酸化和p21蛋白表达。结果与0μmol/L胡桃醌比较,胡桃醌(30、40、50μmol/L)显著抑制RA-FLSs细胞活力和增殖能力,促进其凋亡和caspase-3活性(P0.01);30μmol/L胡桃醌能显著抑制Akt磷酸化,促进p21蛋白表达(P0.01)。与30μmol/L胡桃醌比较,SC79(1.0μg/mL)与30μmol/L胡桃醌共同处理时,p21蛋白表达显著下降,并能促进RA-FLSs细胞凋亡,抑制其增殖(P0.01)。结论胡桃醌可通过调节Akt磷酸化来调控p21表达,进而调节RA-FLSs增殖和凋亡过程,为类风湿关节炎的治疗提供价值。     

艾灸对类风湿关节炎模型大鼠跖关节中细胞因子的影响

目的 观察艾灸"肾俞"、"足三里"对类风湿关节炎(Rheumatoid arthritis,RA)模型大鼠跖关节中细胞因子的影响,为艾灸的局部抗炎免疫作用机制提供依据.方法 采用风、寒、湿环境因素结合弗氏完全佐剂的模型复制方法;将120只Wistar大鼠随机分为正常组、模型组、药物组、针刺组、艾灸组、CO2激光组六组,...     

针灸治疗类风湿性关节炎的临床思路探讨

通过对历代针灸治疗痹证（类风湿性关节炎RA）思路的回顾与分析，结合现代对RA的认识，提出针灸治疗RA应分期进行，早期注重健运脾胃，以治其标，中（晚）期补益肝肾，以防其变，缓解期维护正气，以固其本的治疗思路，并以X线作为重要分期依据，以期对针灸治疗RA的临床思路有所助益。     

电针治疗椎动脉型颈椎病的临床体会

目的:探讨电针对于椎动脉型颈椎病的临床治疗。方法:选百会、风池、天柱、风府、夹脊穴、丰隆、阳陵泉进行电针治疗。结果:连续治1个月,临床治愈。结论:电针治疗椎动脉型颈椎病疗效确切。     

抗风止痛宁对类风湿关节炎大鼠血清中炎症因子的影响

目的研究抗风止痛宁搽剂对类风湿关节炎大鼠血清中炎症因子的影响。方法将40只SD大鼠随机分为空白组、模型组、对照组和治疗组,每组10只。除空白组外,各组均采用胶原诱导性关节炎模型制造方法造模,24 h后对照组给予地塞米松磷酸钠灌胃治疗,治疗组给予抗风止痛宁搽剂外用治疗,观察各组右足肿胀出现时间及全部肿胀的时间并进行对比,第28天采血后检测血清中IL-1β和TNF-α含量并进行对比分析。结果模型组大鼠右后足第10天出现肿胀,14 d全部肿胀;对照组第16天出现肿胀,21 d全部肿胀;治疗组第16天出现肿胀,22 d全部肿胀。模型组与空白组相比,血清IL-1β和TNF-α水平均明显升高;治疗组及对照组与模型组相比,血清IL-1β和TNF-α的水平均明显降低;治疗组与对照组相比,血清IL-1β和TNF-α的水平无明显差异。结论抗风止痛宁搽剂对类风湿关节炎具有一定的治疗作用,其作用途径可能与干预血清中IL-1β和TNF-α的水平有关。     

抗风止痛宁对类风湿关节炎大鼠血清中炎症因子的影响

目的研究抗风止痛宁搽剂对类风湿关节炎大鼠血清中炎症因子的影响。方法将40只SD大鼠随机分为空白组、模型组、对照组和治疗组,每组10只。除空白组外,各组均采用胶原诱导性关节炎模型制造方法造模,24 h后对照组给予地塞米松磷酸钠灌胃治疗,治疗组给予抗风止痛宁搽剂外用治疗,观察各组右足肿胀出现时间及全部肿胀的时间并进行对比,第28天采血后检测血清中IL-1β和TNF-α含量并进行对比分析。结果模型组大鼠右后足第10天出现肿胀,14 d全部肿胀;对照组第16天出现肿胀,21 d全部肿胀;治疗组第16天出现肿胀,22 d全部肿胀。模型组与空白组相比,血清IL-1β和TNF-α水平均明显升高;治疗组及对照组与模型组相比,血清IL-1β和TNF-α的水平均明显降低;治疗组与对照组相比,血清IL-1β和TNF-α的水平无明显差异。结论抗风止痛宁搽剂对类风湿关节炎具有一定的治疗作用,其作用途径可能与干预血清中IL-1β和TNF-α的水平有关。     

姜黄素对佐剂性关节炎大鼠成纤维样滑膜细胞增殖的抑制作用

目的：观察姜黄素对佐剂性关节炎大鼠成纤维样滑膜细胞（FLS）细胞增殖、细胞周期和周期蛋白D1的影响.方法：体外原代培养佐剂性关节炎大鼠FLS.取第3代FLS分别加入浓度为10,20,40,80 μmol/L的姜黄素溶液,共同培养24 h、72 h.MTT法检测药物对FLS增殖的影响,流式细胞术分别分析细胞周期和周期蛋白D1.结果：共培养24 h后40、80 μmol/L浓度组的MTT光密度值与空白对照组比较,差异有统计学意义（P〈0.05）,80 μmol/L浓度组G1期细胞所占比例和周期蛋白D1荧光指数与空白对照组差异也有统计学意义（P〈0.05）;共培养72 h 后10,20,40,80 μmol/L各浓度姜黄素组的光密度值、G1期细胞所占比例及周期蛋白D1与空白对照组比较,差异有统计学意义（P〈0.05）.结论：姜黄素能抑制佐剂性关节炎大鼠纤维样滑膜细胞的增殖,使细胞停滞在G1期,该作用可能与降低周期蛋白D1的表达有关.     

灸法对实验性关节炎大鼠足跖肿胀及痛阈的影响

目的:观察比较温和灸与中药对实验性关节炎大鼠足跖肿胀及痛阈的影响.方法:将46只雄性Wistar大鼠随机分为5组,对照组10只,模型组、中药组、艾灸组和西药组每组9只.除对照组外,其他4组采用冷刺激与弗氏佐剂结合诱导大鼠关节炎模型.造模成功后,对照组、模型组不予治疗.其余3组分别接受中药、艾灸和西药治疗,观察大鼠足跖肿胀度及痛阈变化.结果:造模大鼠佐剂注射后足跖均出现明显肿胀.治疗7d后,中药组大鼠足跖仍有明显肿胀;西药组和艾灸组大鼠足跖肿胀度,与中药组比较差异均有统计学意义(均P＜0.05),与模型组比较差异均无统计学意义(均P＞0.05).西药组、中药组和艾灸组大鼠的机械痛阈均明显高于模型组,组间差异均有统计学意义(均P＜0.05);其中中药组高于西药组,组间差异有统计学意义(P＜0.05);艾灸组高于西药组,但组间差异无统计学意义(P＞0.05).西药组大鼠热疼痛缩足阈潜伏期明显长于模型组大鼠,组间差异有统计学意义(P＜0.05);中药组和艾灸组热疼痛缩足阈潜伏期较模型组虽有延长趋势但无统计学意义(P＞0.05).结论:温和灸和中药均能明显提高实验性关节类大鼠的机械痛阈,且有一定的镇痛作用.     

艾灸对佐剂性关节炎大鼠膝关节滑膜组织中调控铁死亡相关因子的影响

目的:观察艾灸对佐剂性关节炎(AA)大鼠滑膜组织p53、胱氨酸/谷氨酸逆向转运蛋白溶质载体家族7成员11(SLC7A11)、谷胱甘肽过氧化物酶4(GPX4)表达的影响,探讨艾灸治疗AA的作用机制.方法:SD大鼠随机分为正常组、模型组、艾灸组和药物组,每组20只.采用风寒湿环境因素结合完全弗氏佐剂建立AA大鼠模型.艾灸组...     

Effects of moxibustion at different times on prostaglandin and vasopressin levels in uterine tissues of rats with dysmenorrhea due to cold-dampness retention

Objective

To observe the effects of moxibustion at different times on prostaglandin E₂ (PGE₂), prostaglandin F_2α (PGF_2α) and arginine vasopressin (AVP), in the uterine tissues of rats with dysmenorrhea due to cold-dampness retention, and to explore the differences and possible mechanisms of moxibustion at different times in easing pain in dysmenorrhea due to cold-dampness retention.

Methods

Forty-three female Wistar rats were randomly divided into a blank control group (n=7), a model group (n=9), a pre-moxibustion group (n=9), an immediate-moxibustion group (n=9) and a pre-moxibustion plus immediatemoxibustion group (n=9). Rat models of primary dysmenorrhea due to cold-dampness retention were established using (0±1) °C ice water immersion method combined with injection of estradiol benzoate for 10 d, followed by injection of oxytocin on the 11th day. Rats in the 3 intervention groups received moxibustion to Shenque (CV 8) and Guanyuan (CV 4), 10 min for each acupoint, once a day. Rats in pre-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling, for 3 continuous days; rats in immediate-moxibustion group were given one time mild moxibustion, immediately after injection of oxytocin on the 11th day during modeling; rats in pre-moxibustion plus immediate-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling till immediately after injection of oxytocin on the 11th day during modeling, for 4 continuous days. The level of PGF_2α in the rat uterine tissues was measured by enzyme-linked immunosorbent assay (ELISA), and the levels of PGE₂ and AVP in rat uterine tissues were measured by radioimmunoassay.

Results

Compared with the blank control group, the levels of PGF_2α and AVP, the PGF_2α/PGE₂ ratio in the model group were significantly increased (P<0.01), and the PGE₂ level was significantly decreased (P<0.01) in the rat uterine tissues in the model group. Compared with the model group, the writhing latency was significantly prolonged, the writhing number and the total writhing score were all decreased in the pre-moxibustion group, the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group (all P<0.01); the levels of PGF_2α and AVP, and the PGF_2α/PGE₂ ratio were all significantly decreased (P<0.05, P<0.01), and the PGE₂ level was significantly increased (P<0.01) in the rat uterine tissues of the 3 treatment groups. Compared with the pre-moxibustion group, the writhing number and the total writhing score were all decreased in the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group (all P<0.01), the writhing latency was significantly prolonged in the pre-moxibustion plus immediate-moxibustion group(P<0.01); the levels of PGF_2α and PGF_2α/PGE₂ ratio were significantly decreased (P<0.05, P<0.01), and the PGE₂ level was significantly increased (P<0.01) in rat uterine tissues in the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group. Compared with the immediate-moxibustion group, the writhing latency was significantly prolonged and the writhing number was decreased (all P<0.05), and the total writhing score was decreased (P<0.01) in the pre-moxibustion plus immediatemoxibustion group; the PGF_2α level and the PGF_2α/PGE₂ ratio were significantly decreased (P<0.01), and the PGE₂ level was significantly increased (P<0.01) in rat uterine tissues in the pre-moxibustion plus immediate-moxibustion group.

Conclusion

Moxibustion at different times all can produce obvious analgesic effects on dysmenorrhea due to cold-dampness retention in rats, and pre-moxibustion plus immediate-moxibustion ranks the top. The mechanism of this analgesic effect may be via the regulation of abnormal PGF_2α, PGE₂ and AVP levels, to effectively inhibit the spastic contraction of uterine smooth muscle in dysmenorrhea rat, thereby improving the ischemia and hypoxia in uterus.

     

隔物温和灸对类风湿关节炎肾虚证的改善作用

目的：观察隔物温和灸对类风湿关节炎（ RA）肾虚证的改善作用。方法将70例RA肾虚证患者随机分为2组,对照组35例予来氟米特片治疗,治疗组35例在对照组治疗基础上应用隔物温和灸。治疗3个月。观察2组治疗前后肾虚证积分变化及疾病活动性指标变化。结果2组治疗后肾虚证积分均较本组治疗前降低（P＜0．05）,且治疗组降低更明显（P＜0．05）。2组治疗后红细胞沉降率（ESR）、C反应蛋白（CRP）及28个关节疾病活动评分（DAS 28）均较本组治疗前降低（P＜0．05）,且治疗组降低更明显（P＜0．05）。结论隔物温和灸可有效降低RA肾虚证患者肾虚证积分,降低疾病活动性指标。     

针灸治疗类风湿性关节炎的研究进展

综述了采用针刺、温针、穴位注射、蜂针及综合疗法等治疗类风湿性关节炎的研究进展。提示 :针灸治疗已从单纯的临床观察进入到实验研究阶段 ,今后应进一步开展针灸治疗的机理研究工作。