口腔扁平苔藓浸润细胞免疫组化分析

这探讨免疫应答在扁平苔藓发病过程中的作用及相互关系，本研究中应用ＡＢＣ技术，用Ｔ３、Ｔ４、Ｔ８、ＩｇＧ单抗及Ｓ－１００蛋白标记病损已浸润细胞。结果表明：病损区以细胞免疫为主，淋巴细胞数量与上皮钉突的增殖程度呈正相关；病损区中郎罕氏细胞明显偏高；基低膜区损害与Ｔ细胞数量呈正相关；ＩｇＧ阳性细胞散在分布于病损区，其阳性物质在基底膜区沉积。提示该疾病以细胞免疫为主，同时有体液免疫应答的参与。     

Ets-1在口腔扁平苔藓中的表达研究

目的 :分析Ets 1(E2 6transformation specific)在口腔扁平苔藓 (OLP)的表达及意义。 方法 :采用免疫组化ABC法检测Ets 1蛋白在 2 0例口腔扁平苔藓和 8例正常口腔黏膜组织中的表达。结果 :70 % (14 /2 0 )的口腔扁平苔藓病例中Ets 1呈阳性表达 ,明显高于正常黏膜组织 ,两者比较有显著性差异 (P <0 .0 5 )。阳性表达率在溃疡型OLP与斑块型OLP间亦有显著性差异 (P <0 .0 5 ) ,并与病程相关 (P <0 .0 5 )。结论 :Ets 1在口腔扁平苔藓中过表达并与其发病有关。     

口腔扁平苔藓人乳头瘤病毒的检测

目的 探讨口腔扁平苔藓（OLP）和人乳头瘤病毒（HPV）的关系，从而为确定OLP的原因，诱因和发病机理提供理论依据。方法 应用通用引物（General Primer,GP)介导的PCR技术检测OLP和正常口腔黏膜的HPVDNA，并对HPV－16DNA进行特异性检测。结果 30例OLP检测到25例HPV－6，11，33DNA阳性，而对照组检测到5例，两者相比有显著性差异（P＜0．05）；OLP组检测到3例HPV－16，18DNA阳性，对照组1例，两者相比无显著性差异（P＞0．05）。结论 OLP是以低危型HPV感染为主，其中糜烂型OLP可检测到较高高危HPV16 DNA。因此，对OLP尤其是糜烂型OLP要进行HPV的检测，从而进行有效治疗。     

口腔扁平苔藓患者红细胞免疫粘附功能初探

采用我国郭氏酵母菌花环法对30例口腔扁平苔藓(OLP)患者及30例健康对照者红细胞免疫粘附功能进行了初步观察,以了解红细胞是否参与了 OLP 的免疫过程。结果发现,OLP 患者红细胞 C3b受体花环率及循环免疫复合物(CIC)花环率均较健康对照组升高。说明 OLP 患者红细胞免疫粘附功能较正常人增强,推测这可能是由于 CIC 增高后红细胞免疫功能的代偿性反应。这对于防止 CIC 在局部沉积造成体液免疫性损伤具有重要意义,为 OLP 细胞介导的免疫损伤提供了又一证据。     

CD44在口腔扁平苔藓中的表达及意义

目的 探讨口腔扁平苔藓病变中粘附分子CD44H、CD44V3表达的意义。方法 应用免疫组织化学SP法对62例口腔扁平苔藓，其中糜烂型扁平苔藓14例，非糜烂型扁平苔藓48例，21例慢性盘状红斑狼疮，10例正常粘膜CD44H、CD44V3的表达进行观察。结果 扁平苔藓病损区组织的染色以基底细胞、棘层细胞下调明显。结论 CD44参与口腔扁平苔藓的发病过程。     

口腔扁平苔藓患者病变组织和唾液中纤维连结蛋白的研究

为探讨纤维连接蛋白在口腔扁平苔藓发病中的作用，作者采用ＡＢＣ免疫组化技术，并结合图像分析及放射免疫测定技术，检测了３０例口腔扁平苔藓患者病变组织和唾液中纤维连结蛋白的变化。结果显示：此种患者病变组织中纤维连结蛋白含量虽未发生明显改变，但分布形式有变化；患者唾液中纤维连结蛋白呈低水平趋势。推测口腔扁平苔藓时，病变组织的上皮细胞产生的纤维连结蛋白向外分泌发生了障碍，疾病的反复发作和糜烂的迁延不愈很可能与纤维连结蛋白的变化有关。     

口腔扁平苔藓患者免疫功能的实验研究

目的 探讨口腔扁平苔藓（OLP）的发病机理。方法 对口腔扁平苔藓患者64例及健康成人31例,采用微量全血^3H－TdR掺入法检测T淋巴细胞增殖功能;酵母菌花环法检测红细胞免疫粘附功能;采用单向免疫扩散法测定血清中免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。结果 OLP患者较健康成人外周血T淋巴细胞增殖功能显著降低,每分钟脉冲数（CPM）（P＜0．05）,刺激指数（SI）（P＜0．001）;红细胞C3b受体花环形成率（RBC－C3bRR）显著下降（P＜0．01）,红细胞免疫复合物花环形成率（RBC－ICR）无明显差异;血清中C3、C4含量无明显差异,IgA、IgG含量显著降低（P＜0．001）,而IgM含量则明显升高（P＜0．01）。结论 OLP的发生可能与机体免疫功能紊乱有关。     

口腔扁平苔藓病损区白细胞介素12p40和干扰素-γ的表达及意义

目的 探讨白细胞介素12p40(IL-12p40)及干扰素-γ(IFN-γ)在口腔扁平苔藓(OLP)病损形成及发展中的意义.方法 正常口腔黏膜组织11例,OLP组织43例,采用免疫组织化学Envision二步法检测IL-12p40和IFN-γ的表达情况,分析其与OLP患者临床病理特征的关系.结果 1)OLP组IL-12p40与IFN-γ的阳性表达率均高于正常对照组,差异有统计学意义(P<0.05).2)在OLP组中,IL-12p40阳性表达率在IFN-γ阳性组高于IFN-γ阴性组,差异有统计学意义(X2=5.828,P=0.016),IFN-γ和IL-12p40表达之间存在正相关关系(r=0.357,P=0.019).3)IL-12p40的阳性表达率在病程短于6个月组高于病程长于6个月组组(X2=7.935,P=0.005);基底细胞液化程度高分组中的IFN-γ阳性表达率高于低分组(X2=9.070,P=0.011).结论 IL-12可能通过促进典型Th1型细胞因子IFN-γ的产生参与了OLP病损区的病理损伤过程,在OLP局部病损形成初始阶段起到关键作用.     

口腔扁平苔藓病损区白细胞介素12p40和干扰素-γ的表达及意义

目的 探讨白细胞介素12p40(IL-12p40)及干扰素-γ(IFN-γ)在口腔扁平苔藓(OLP)病损形成及发展中的意义.方法 正常口腔黏膜组织11例,OLP组织43例,采用免疫组织化学Envision二步法检测IL-12p40和IFN-γ的表达情况,分析其与OLP患者临床病理特征的关系.结果 1)OLP组IL-12...     

Hepatitis C virus infection in Italian patients with oral lichen planus: a prospective case-control study

To assess the aetiology of liver disease associated with lichen planus, we prospectively studied 70 consecutive newly diagnosed patients with oral lichen planus (OLP) living in northwest Italy (Piemonte) and 70 controls matched for age and sex with other oral keratoses coming from the same district. Twenty-two patients with OLP (3.4%) and 9 controls (12.9%) were found to be affected by chronic liver disease (CLD) ( P =0.014). In sixteen of the 22 OLP patients with CLD the liver disease was hepatitis C virus (HCV)-related. whereas 2 of the 9 controls had a HCV-related CLD ( P =0.016). In another OLP case, liver damage was related to a combination of HCV and alcohol abuse. The prevalence of HCV antibodies in the whole OLP group (27.1%) was significantly higher than in controls (4.3%) ( P =0.014). whereas no difference was found between the OLP and control groups regarding hepatitis B virus markers and other common causes of CLD. HCV infection was more frequently found in patients with erosive OLP (58.8%) than in patients with non-erosive OLP (13.2%) ( P =0.004). Serum HCV-RNA was detected by polymerase chain reaction (RT-PCR) in the majority (93.7%) of OLP patients who had HCV antibodies. Excluding OLP and control patients with HCV markers, there was no difference between the two groups regarding frequency of CLD. Our data show that HCV is probably the main pathogenic factor in liver disease of Italian patients with OLP. and suggests that HCV could be involved in the pathogenesis of OLP.     

Hepatitis C virus infection and oral lichen planus: a report from the Netherlands

The reported frequency of anti-hepatitis C virus (HCV) antibodies in groups of cutaneous lichen planus (CLP) and/or oral lichen planus (OLP) patients varies from about 4% to 65%. Most of these studies have been performed in countries with a high overall prevalence of HCV infection in the general population, such as the southern European countries and Japan. Limited data are available from areas with a low prevalence of HCV infection. Therefore, we investigated the prevalence of HCV infection in a series of 55 patients with OLP in the Netherlands, which apparently has a low prevalence of HCV infection. None of the 55 patients revealed serological evidence of antibodies to HCV. The present data suggest that HCV infection in OLP patients in the Netherlands is probably not very common. A larger study group with a sex- and age-matched control group is required to advise against routine serological examination for HCV infection in Dutch OLP patients.     

Human papilloma virus in erosive oral lichen planus

Several types of human papilloma viruses (HPV) have been associated with benign and malignant squamous cell tumours of mucosal epithelium. To identify HPV in erosive oral lichen planus (OLPe), considered as a premalignant lesion, tissues from 20 patients were examined by Southern blot hybridization with 32P-labeled HPV DNA probes. Type 11 was found in 6 of the lesions while HPV types 6, 16 and 18 were not detected in any of the tissues examined. Using a type-specific polymerase chain reaction (PCR) assay for HPV-6, 11, 16 and 18, HPV-11 was detected in 8 of the samples (all of those positive by Southern blot), and, in addition, HPV-6 was found in 5 samples and HPV-16 in 3 samples. Overall, by the more sensitive PCR assay, 65% of samples were positive for HPV DNA. The finding of HPV DNA in many of the samples using two different techniques indicates a high prevalence of HPV in the OLPe afflicted oral mucosa. However, the role of HPV in the pathogenesis of OLPe has yet to be determined.     

Candida in patients with oral lichen planus

The presence of yeasts in oral lichen planus (OLP) lesions was studied in cultivations from 41 OLP patients and by histological examination in 39 of these cases. The histological features of OLP were also recorded. The cultivation results were compared with those of a randomly selected, age- and sex-matched control group without mucosal changes. The extent of growth was recorded as "sparse" (1-10 colonies), "model-rate" (11-40 colonies), or "heavy" (greater than 40 colonies). Yeasts were found to be present on cultures or histologic sections from a total of 19 OLP patients (46%). "Moderate-heavy" growth was obtained in 29% of the OLP cases but in only 7% of the control group. Candida albicans accounted for over 80% of the yeasts. The histological examination revealed only 3 cases of invasive fungal growth. Regarding the criteria of OLP, hyperortho- or hyperparakeratosis and a band-shaped subepithelial infiltration of lymphocytes were present in all specimens, basilar liquefaction degeneration in 87% and an eosinophilic zone in 77%. Local treatment with amphotericin B (Fungizone) in 18 OLP patients with positive findings resulted in subjective relief of symptoms in 89% of the patients. Clinical improvement was seen in 94%. The presence of Candida was significantly correlated to low secretion rate in unstimulated saliva.