兔胆囊结石形成过程中脂代谢和载脂蛋白变化研究

采用高胆固醇膳食诱发兔胆囊胆固醇结石模型，分别对高胆固醇膳食后１，２，３，４周组及对照组（每组１０只）血清脂质，载脂蛋白变化进行了研究。结果显示：①高胆固醇膳食后１周开始出现胆囊结石，２、３和４周组分别有４只、６只和７只兔出现胆囊结石；②血清总胆固醇、甘三油酯、磷脂、低密度脂蛋白胆固醇及极低密度脂蛋白胆固醇逐渐明显升高（与对照组比较Ｐ＜０．０５），高密度脂蛋白胆固醇及其亚组份有降低趋势，但无显著性差异（Ｐ＞０．０５）；③血清载脂蛋白ａｐｏＢ１００逐渐明显升高（Ｐ＜０．０５），以３、４周组明显；ａｐｏＡⅠ逐渐降低，以４周组最明显（Ｐ＜０．０５）；ａｐｏＣⅡ及ａｐｏＣⅢ逐渐明显升高，均在２周后最明显（Ｐ＜０．０５）。提示上述结果均在胆囊结石形成过程中起着重要作用     

胆囊结石易患人群筛选方法的研究

作者研究了５１例胆囊结石患者和９例非胆石患者的血清脂质和胆汁胆固醇饱和指数。结果表明：（１）结石组与非结石组之间ＣＳＩ的差异无显著意义。（２）用逐步判别法对两组血清脂质指标进行分析，提示由ａｐｏＡ－１，ＨＤＬ－Ｃｈ和ＬＤＬ－Ｃｈ所构成的判别函数（Ｐ＜０．０５），对胆石与非胆石人群有良好的区分作用，其内部检验符合率和外推检验符合率分别为８４％和７０％。     

载脂蛋白B基因多态性与胆固醇结石病关系的研究

目的 探讨载脂蛋白Ｂ（ａｐｏＢ）基因多态位点Ｉｎｓ／Ｄｅｌ,ＸｂａＩ基因多态与胆固醇结石病的关系。方法 应用聚合酶链反应法和限制性片段长度多肽法,观察这两个多态位点１０１例胆固醇结石患者和５０例对照者的分布,结果 结石ＸｂａＩ多态位点的Ｘ＾＋等位基因频率显著高于对照组（Ｘ＾２＝４．０５,Ｐ〈０．０５）,Ｘ＾＋Ｘ＾－基因型分布也显著高于对照组（Ｘ＾２＝４．５０,Ｐ〈０．０５）,结石组Ｉｎｓ／Ｄｅｌ多     

妊娠妇女妊娠过程中血脂及载脂蛋白的变化

目的了解不同孕周正常妊娠妇女及未孕育龄妇女(对照组)的血脂及载脂蛋白的变化.方法TC、TG、HDL-C及LDL-C采用酶学直接测定法,ApoAI和ApoB采用免疫透射比浊法.结果TC和TG从孕9周起迅速升高,与对照组差异有显著性意义(两者均P＜0.05),TG持续升高至孕末期,TC于第30周到达最高值,此后处于高水平状态.HDL-C和ApoAI于妊娠前半期迅速升高(P＜0.05),后半期变化不大,LDL-C和ApoB早孕阶段无明显改变,分别于第16周和第14周后明显上升(P＜0.05)和(P＜0.01)至第30周达到稳态.结论正常妊娠妇女所有脂代谢指标均值于妊娠20周后均高于正常参考值范围,孕妇处于生理性高血脂状态.     

甲亢患者治疗前后血脂和载脂蛋白的变化

观察３０例甲状腺功能亢进症患者药物治疗前后血脂和载脂蛋白变化,ＧＤ患者治疗前血清总胆固醇、高密度脂蛋白胆固醇和ＡＰＯＢ１００均明显降低,经抗甲状腺药物治疗亢缓解后,上述指标恢复正常。     

冠心病患者血脂,载脂蛋白Al和B100的变化

研究结果已证实高胆固醇血症是冠心病的主要独立危险因素;低高密度脂蛋白胆固醇（HDLQ也是重要的危险因素,而HDL－C之主要脂蛋白成份AI（ApoAI）是指示冠状动脉粥样硬化风险度的良好指标。我们测定了冠心病患者的血胆固醇（TC）,甘油三酯（TG）,高密度脂蛋白（HDLC）,载脂蛋白（Apo）AI及B;。。的浓度,并以健康人作对照,以着重探讨HDL－C及ApoAI＼ApoB100的浓度与冠心病的关系。1对象与方法1．l对象冠心病组：82例,男性44例,女38例,平均年龄63．39士9．5岁（44～82岁）。均符合1979年WHO冠心病的诊断标准。其中陈…     

载脂蛋白E基因多态性和胆囊结石形成关系的研究进展

载脂蛋白E（ApoE)是一种影响脂蛋白代谢的遗传多态性蛋白，它有E2，E3和E4 3种异构体，分别由3个等位基因ε2，ε3和ε4编码。不同表型的ApoE通过调节脂代谢来影响胆囊胆固醇结石的形成，其中ApoE4是促进胆囊结石发生的高危因子。     

肝细胞高密度脂蛋白受体在兔胆囊结石形成中的动态变化

采用高胆固膳食诱发兔胆囊固醇结石模型,分别对高胆固醇膳食后１、２、３、４周及对照组高密度旧白受体（ＨＤＬｒ）活性,胆汁中胆汁酸及胆固醇变化进行了研究。结果发现：（１）高胆固醇膳食后１周肝细胞ＨＤＬｒＢｍａｘ明显增大而Ｋｄ值明显变小,与对照组相比有显著性差异（Ｐ〈０．０５）,２周组两者降至对照组水平,３周及４周组Ｂｍａｘ明显变小而Ｋｄ值明显增大,有显著性差异（Ｐ〈０．０５）,（２）１周量汁中总胆汁酸     

兔胆囊结石形成过程中肝细胞低密度脂蛋白受体变化的研究

In order to study the mechanism of cholesterol gallstone formation through rabbit model which was induced by high cholesterol diet, we investigated the LDL receptor activity of hepatocytes binding to 125I-LDL in different phases, namely 1, 2, 3 week group and 4 week, and in a control group besides. In this animal experiment, cholesterol gallstones were induced at 2 week, 3 week and 4 week groups in 4/10, 6/10, and 7/10 cases respectively. The Bmax values of LDL receptor of hepatocytes binding to 125I-LDL decreased significantly in 3 week and 4 week groups (vs 1 week and control groups, P < 0.05). The kd values became increased in 3 week and 4 week groups (vs 1 week and control group, P < 0.05), which suggested that the activity of LDL receptor decreased gradually. In conclusions owing to the intake of high cholesterol diet with the passage of time, the decreased activity of LDL receptor of hepatocytes would reduce the synthesis of bile acid.     

肝性脂酶及脂蛋白脂酶在兔胆囊结石形成过程中的变化及意义

为研究肝脂酶及脂蛋白脂酶在胆固醇结石成石过程中的变化及其对成石的影响,采用高胆固醇膳食诱发兔胆囊胆固醇结石模型,观测对照组及高胆固醇膳食１、２、３、４周组动物血浆脂蛋白脂酶（ＬＰＬ）、肝脂酶（ＨＬ）活性、血浆脂蛋白胆固醇及胆汁中甘氨胆酸（ＧＣＡ）、甘氨脱氧胆酸（ＧＤＣＡ）、胆固醇的变化。结果显示：随着进食高胆固醇膳食时间的增加,血中ＬＰＬ活性增高明显（ｐ〈０．０５）;ＨＬ活性也逐渐升高,３周组及４     

人血清载脂蛋白A—Ⅳ的纯化,鉴定及逆向转运胆固醇的研究

In order to study the function of apoA-IV, human serum apoA-IV was isolated and purified. LDS was isolated with NaBr density gradient ultracentrifugation and then incubated with intralipid according to the method of Weinberg and Scanu. Protein complex containing apoA-IV was obtained. The apo-IV was purified from protein complex by preparative SDS-PAGE, electroelution and removal of SDS. ApoA-IV thus prepared gave a single band on SDS-PAGE with a MW of 46 kD. Amino acid composition and isoelectric focusing pI (5.27 and 5.47) of apoA-IV were similar to those reported in the literature. The effects of apoA-IV and liposome (apoA-IV: DMPC) on cholesterol efflux from hum skin fibroblast were studied. There was no significant difference (P > 0.5) between apoA-IV and control but a very significant difference (P < 0.001) between liposome (apoA-IV:DMPC) and control, suggesting that liposomes (apoA-IV:DMPC) play a role in reverse cholesterol transport. The results may offer a new approach for the prevention and treatment of atherosclerotic coronary heart disease.     

免胆囊结石成石过程中脂代谢的变化及高密度脂蛋白制剂对其影响 …

为研究高胆固醇食诱发兔胆囊胆固醇结石模型的脂代谢变化,以及高密脂蛋白对其脂代谢及成石的影响,将动物随机分为对照组,高胆固醇食组及高胆固醇膳食＋ＨＤＬ注射组,观察各组血浆脂质,胆汁中胆固醇,血卵磷脂胆固醇酰基转移酶活性及血和胆汁中胆汁酸及成率的变化 。     

载脂蛋白B基因多态性及血脂成分与胆石病关系的研究

目的 观察载脂蛋白B(ApoB)基因XbaI、EcoR I位点多态性、血脂水平和胆石病之间的关系。方法 通过病例对照研究设计,采用PCR—RFLP技术对106例胆石病患者和105例对照的进行基因和血脂水平分析比较。结果 胆石病组和对照组ApoB基因Xba I位点X X-、X-X-基因型构成显著不等;胆石病组X 等位基因频率显著高于对照组(0．104与0．052);X X-基因型者,胆石病组的低密度脂蛋白胆固醇(LDLc)、ApoB水平显著高于对照组,HDL水平显著低于对照组。胆石病组和对照组ApoB基因EcoR I位点E E-和E E 基因型频率构成差异有显著意义,胆石病组E-等位基因频率显著高于对照组;E E 基因型者,胆石病组LDLc、ApoB水平显著高于对照组,HDLc水平显著低于对照组。在胆石病组中E E-基因型LDLc水平高于E E 基因型(P=0．059)。结论 胆石病发生与ApoB基因Xba I、EcoR I位点多态性存在关联,X 和E-等位基因的变异影响脂质代谢,从而有利于胆石的形成。     

Gastrointestinal migrating motor complex during early stage of cholesterol gallstone formation in guinea pigs

Objective： To determine the changes of gastrointestinal migrating motor complex （MMC） during the early stage of cholesterol gallstone formation. Methods：Totally 45 healthy adult guinea pigs were randomly and equally divided into 3 groups, that is, normal control, group B （1% cholesterol diet for 2 weeks） and group C （1% cholesterol diet for 4 weeks）. Three pairs of silver electrodes were inserted into the gastric antrum, duodenum and jejunum of every animal respectively. Record of gastrointestinal MMC of these guinea pigs were recorded and analyzed. Gallbladder bile was taken to detect the formation of cholesterol gallstone. Results： There were only 29 guinea pigs living. The MMC cycle time and the duration of phase Ⅱ were prolonged significantly and gradually compared with the control group （P〈0.01）, but the duration of phase Ⅲ became significantly and gradually shorter. Conclusion： During the early stage of cholesterol gallstone formation, the MMC cycle time and the duration of phase Ⅱ are prolonged and the duration of phase Ⅲ is shortened.     

家兔胆石模型肝组织固醇携带蛋白2表达的意义

目的:复制家兔胆固醇结石模型,检测模型家兔肝组织固醇携带蛋白2(SCP2) mRNA的表达. 方法:健康北京大耳白家兔16只,随机分为2组,每组8只:对照组喂普通饮食,模型组喂高胆固醇饮食(含12 g/kg胆固醇). 4 wk时剖杀动物,采用半定量RT-PCR检测SCP2 mRNA在家兔肝组织的表达. 结果:对照组无结石形成;模型组成石率达87.5%. 与对照组相比,模型组动物肝组织SCP2 mRNA表达升高(0.79±0.17 vs 0.44±0.07,P＜0.05). 结论:高胆固醇膳食致家兔胆囊结石形成时肝组织SCP2 mRNA表达升高.