成都地区437例健康男性中老年人血清载脂蛋白CⅡ,CⅢ及E水平分析

Apolipoprotein (apo) C II, C III and E levels of 437 male healthy subjects aged 40-70 [with fasting serum triglyceride (TG levels < 2.26 mmol/L, total cholesterol (TC) levels < 6.21 mmol/L and plasma glucose levels < 6.10 mmol/L] in Changdu area were determined by radial immunodiffusion assay (RID). The results showed that the concentrations of apoC II, C III and E were 41.6 +/- 13.1, 112.8 +/- 31.0 and 38.7 +/- 8.2 mg/L (mean +/- s) respectively. No difference in the serum levels of apoC II, C III and E was noted between the men of different ages. The results of the linear correlation showed that there was a positive correlation of apoC II, C III, E with TG, TC, LDL-C; of apoC II, C III with BMI; and of apoE with ages.     

中老年男性血糖与血清脂质及载脂蛋白的关系

OBJECTIVE: To gain an insight into the relation of plasma glucose levels to lipids and apolipoproteins levels. METHODS: Fasting 12-14 h blood glucose (BG), high density lipoprotein cholestrol(LDL-C), apolipoproteins(apo) A I, A II, B100, C II, C III and E levels were measured in 735 men aged 40-70 in Chengdu area. The subjects studied were divided into three groups by the plasma glucose levels: 1. normal group (S1), 2. decreased glucose tolerance group (S2), 3. diabetes mellitus (DM) group (S3). RESULTS: Among 735 cases studied, groups S2 and S3 had more hyperlipidemia than group S1 (64%, 45% and 32.2% respectively). The fasting serum mean TG levels in groups S2 (3.62 +/- 2.52 mmol/L) and S3 (4.45 +/- 3.82 mmol/L) group were significantly higher than that in group S1 (1.92 +/- 1.39 mmol/L) (P < 0.01 and P < 0.01), and the mean TG levels in groups S2 (5.68 +/- 1.15 mmol/L) and S3 (5.68 +/- 1.12 mmol/L) were higher than that in group S1 (5.13 +/- 1.06 mmol/L) (P < 0.05, P < 0.05). The serum apo B100, C II, C III and E levels in groups S2 and S3 were significantly higher as compared with that in the normal group (P < 0.01, P < 0.05). The correlation analysis indicated that there was a positive correlation of BG with serum TG in groups S1, S2 and S3 whose correlation coefficients (r) were 0.1800, 0.1436 and 0.3212 respectively (P < 0.001); and a negative correlation with HDL-C levels in groups S2 and S3 (r = -0.2534 and -0.1477, P < 0.001). The plasma glucose levels were positively correlated with apo B100, C III and E levels in groups S1, S2 and S3, P < 0.001), with apo C II in groups S1 and S2 (P < 0.001, P < 0.01); and negatively correlate to apoA I only in group S2 (r = -0.1182, P < 0.01). CONCLUSION: The results suggest that serum TG, TC, HDL-C, apoB100, C II, C III and E levels be the main factors that related to plasma glucose levels.     

中国人Ⅱb型高脂蛋白血症apoE基因多态性的研究

目的 探讨中国人Ⅱb型高脂蛋白血症患者载脂蛋白E（apoE)基因多态性及其与血脂和载脂蛋白水平的关系。方法 采用聚合酶链反应－限制性酶切片段长度多态性法（PCR－RFLP）,分别对74例Ⅱb型高脂血症患者及230例血脂正常者apoE基因型、空腹血脂及载脂蛋白AⅠ、AⅡ、B100、CⅡ、CⅢ、E进行了全面分析。结果 Ⅱb型高脂血症患者的体重指数（BMI）,血清甘油三酯（TG）,总胆固醇（TC）,低密度 蛋白胆固醇（LDLC）,非高密度脂蛋白胆固醇（nHDLC),载脂蛋白AⅡ、B100、CⅡ、CⅢ、E水平及TG／HDLC比值均较对照组显著升高（P＜0．001）;血清高密度脂蛋白胆固醇（HDLC）水平及apoE/apoCⅢ比值比显著降低（P＜0．05）。Ⅱb型高脂血症组与对照组apoE基因型及等位基因频率分布均以E3／3和ε3最高,Ⅱb型高脂血症组的ε2等位基因有增高的趋势,而ε4等位基因有降低的趋势（P＞0．05）,携带ε2等位基因组血清TG、apoⅡ,apoE水平及apoE/apoCⅢ比值较E3／3基因型组显著升高,而携带ε4等位基因组血清TC、nHDLC及apoE水平较E3／3基因型组显著升高（P＜0．001）。结论 ε2及ε4等位基因与Ⅱb型高脂蛋白血症患者的血清TG、TC、nHDL,apoCⅡ及apoE水平升高有关。     

Apolipoprotein C III gene Sst I polymorphism in patients with endogenous hypertriglyceridemia in Chinese population]

OBJECTIVE: To investigate the apoC III gene Sst I polymorphism and its relationship with serum lipids and apolipoproteins (apo) levels in patients with endogenous hypertriglyceridemia in Chinese population. METHODS: The genotype and allele frequency of apoC III gene Sst I polymorphism was assayed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Serum lipids were measured by enzymatic kits and apolipoproteins A I, A II, B100, C II, C III and E were measured by RID kits developed by Apolipoprotein Research Unit of WCUMS in 176 HTG patients whose fasting serum TG levels were > or = 2.26 mmol/L and in 199 healthy subjects whose fasting serum TG levels were < 1.82 mmol/L and TC levels < 6.2 mmol/L from a population of Chinese Han nationality in Chengdu area. RESULTS: In both HTG group and control group, S1 allele was the major allele and homozygous S1S1 genotype was the most frequent one. The frequency of S2 allele was significantly higher than that reported in Caucasians (0.289 vs 0.06-0.16, P < 0.05). No differences were found in apoC III gene Sst I polymophism in the HTG group when compared with the control group (0.287 vs 0.289, P > 0.05). The genotype of S2S2 was not associated with higher TG and apolipoproteins levels when compared with the genotypes of S1S1 and S1S2 (P > 0.05). CONCLUSION: These results suggest that the Sst I polymorphism of the apoC III gene was not associated with endogenoushypertriglyceridemica in Chinese population.     

Apolipoportein E polymorphism, serum lipids and apolipoproteins of 362 Han national subjects in Chengdu area]

This investigation was conducted to observe the frequency distribution of apoE phenotypes and alleles and to explore the relationship between apoE polymorphism and plasma lipids or apolipoproteins in Chinese population. ApoE phenotypes were assayed by isoelectric focusing and immunoblotting with serum. Serum lipids and apoA I, B100, C II, C III, E were determined in a random subset of 362 subjects including 268 males and 94 females with a mean age of 43.7 +/- 12.3 yrs from a population of Han Nationality in Chengdu area. The results showed that the frequencies of apoE phenotypes and alleles were: E3/3 72.93%, E2/3 12.98%, E3/4 11.33%, E2/4 1.38%, E4/4 1.38%, E2/2 0.00%; epsilon 3 0.8508, epsilon 2 0.0718, epsilon 4 0.0774. The results also showed that the apo E2(E2/3 + E2/2) group had lower levels of serum TC and apoB100 (P < 0.05) and a higher level of serum apoE (P < 0.001) when compared with the apoE3(E3/3) or apoE4(E3/4 + E4/4) group. No significant difference was observed in TG, apoA I, apoC II, and apoC III levels among the apoE2, E3 and E4 groups (P > 0.05).     

The abnormal changes of apolipoprotein(s) in patients with type 2 diabetes mellitus]

OBJECTIVE: To investigate the changes of the apolipoproteins(apoA I, A II, B100, C II, C III, E) in patients with type 2 diabetes mellitus. METHODS: The levels of fasting plasma glucose(FBG), insulin, TG, TC, apoA I, A II, B100, C II, C III, E were all measured in 127 non-diabetic subjects and 143 type 2 diabetic patients (20 associated with coronary heart disease (CHD) and 66 associated with hypertriglyceridemia(HTG), 55 associated with hypercholesterolemia). RESULTS: In male type 2 diabetic patients, the levels of FBG, WHR and apoC II were significantly higher (P < 0.05) and apoA I, A II levels were significantly lower (P < 0.05) than those in male non-diabetic subjects. In female type 2 diabetic patients, the levels of FBG, BMI, WHR, TG significantly elevated while HDL-C, apoA I, A II levels significantly decreased as compared with those in female non-diabetic subjects. In type 2 diabetic group, the levels of WHR, FBG and TG in HTG patients were elevated significantly as compared with those without HTG, and the levels of HDL-C, apoA I and apoA II were decreased; the levels of WHR, TG, TC, apoB100, C II, C III, E in patients with HTC were significantly higher than those whose cholesterol levels were normal. In patients with CHD, the levels of fasting insulin, apoB100, apoC II and apoE were significantly higher than those in patients without CHD, and the levels of HDL-C and apoA II were decreased significantly. CONCLUSION: Abnormal changes of apo(s) in type 2 diabetes mellitus may be a cause of type 2 diabetes associated with HTG and CHD.     

中国人Ⅱb型高脂蛋白血症apoE基因多态性的研究

目的　探讨中国人 b型高脂蛋白血症患者载脂蛋白 E(apo E)基因多态性及其与血脂和载脂蛋白水平的关系。方法　采用聚合酶链反应 -限制性酶切片段长度多态性法 (PCR- RFL P) ,分别对 74例 b型高脂血症患者及 2 30例血脂正常者的 apo E基因型、空腹血脂及载脂蛋白 A 、A 、B10 0、C 、C 、E进行了全面分析。结果　 b型高脂血症患者的体重指数 (BMI) ,血清甘油三酯 (TG) ,总胆固醇 (TC) ,低密度脂蛋白胆固醇(L DL C) ,非高密度脂蛋白胆固醇 (n HDL C) ,载脂蛋白 A 、B10 0、C 、C 、E水平及 TG/ HDL C比值均较对照组显著升高 (P<0 .0 0 1) ;血清高密度脂蛋白胆固醇 (HDL C)水平及 apo E/ apo C 比值显著降低 (P<0 .0 5 )。 b型高脂血症组与对照组 apo E基因型及等位基因频率分布均以 E3/ 3和 ε3最高 , b型高脂血症组的 ε2 等位基因有增高的趋势 ,而 ε4等位基因有降低的趋势 (P>0 .0 5 )。携带 ε2 等位基因组血清 TG、apo C 、apo E水平及 apo E/ apo C 比值较 E3/ 3基因型组显著升高 ,而携带 ε4等位基因组血清 TC、n HDL C及 apo E水平较 E3/ 3基因型组显著升高(P<0 .0 0 1) )。结论　 ε2 及 ε4等位基因与 b型高脂蛋白血症患者的血清 TG、TC、n HDL C、apo C 及 apo E水平升高有关。     

正常妊娠及妊娠高血压综合征患者血清脂质和载脂蛋白的分析

目的：探讨正常妊娠和妊娠高血压综合征（妊高征）患者血脂和载脂蛋白改变。以及这些改变与妊高征发病的关系。方法：采用酶法和免疫扩散法，对28例妊高征孕妇，31例正常孕妇及24例未孕妇女血脂和载脂蛋白进行了检测。分别测定了甘油三酯（TG），胆固醇（TC），高密度脂蛋白胆固醇（HDL－C），载脂蛋白（apo)AI,B100,CⅡ，CⅢ和E，并计算非HDL－C（nHDLC)和TG/HDL-C秕值，结果：（1）与未孕妇女比较，正常妊娠36－42周妇女血清TG,apoCⅡ及apoCⅢ水平分别升高了3．5倍，2．4倍和2．8倍（P＜0．001），血清TC升高21％（P＜0．01），血清HDL－C未见明显改变，nHDL-C升高33％（P＜0．05），apoA,I,apoB100及apoE分别升高了55％，79％和77％（P＜0．001），TG／DHL－C比值显著增加（P＜0．01），（2）与未孕妇女比较，妊高征组妊娠32－42周妇女血清TG，apoCII及apo CⅢ亦分别升高2＝3倍，4．0倍和2．8倍（P＜0．01），血清TC仅升高27％（P＜0．01），HDL－C升高29％（P＜0．05），nHDL-C升高26％（P＜0．05）；apoAI,apoB100及apoE分别升高52％，90％和67％（P＜0．001），TG／HDL－C比值亦显著增加（P＜0．01），与正常妊娠组比较，妊高征组血脂和载脂蛋白水平平均未见明显差异。（3）不同程度妊高征及正常妊娠对照血脂和载脂蛋白含量的比较显示，妊高征患者各亚组血脂和载脂蛋白的含量不随病情的严重程度而升高。结论：妊娠时胎盘分泌的大量雌性激素是导致正常妊娠及妊高征患者血脂及载脂蛋白显著升高的主要原因，妊娠时血脂及载脂蛋白的升高可能与妊高征的发病无关。     

兔胆囊结石形成过程中脂代谢和载脂蛋白变化研究

采用高胆固醇膳食诱发兔胆囊胆固醇结合模型，分别对高胆固醇膳食后１，２，３，４，周组及对照组血清脂质，载脂蛋白变化进行了研究。结果显示：１．高胆固醇膳食后１周开始出现胆囊结石２，３，和４周分别有４只，６只和７只兔出现胆囊结石；２．血清总胆固醇，甘三油酯，磷脂，低密度脂蛋白胆固醇及极低密度脂蛋白胆固醇逐渐明显升高，高密度脂蛋白胆固醇及其亚组份在降低趋势，但无显著性差异；     

Prevalence of dyslipidemia in the Iraqi adult population

OBJECTIVE: To establish the prevalence of dyslipidemia in the Iraqi adult population in Mosul, Iraq, according to the different recommended criteria. METHODS: The study was carried out from October 2003 to April 2004, with 871 apparently healthy volunteers (413 males, 458 females) aged 20-70 year ([mean +/- SD] 41.2 +/- 13.8 year). Fasting blood specimens were collected from all subjects for measurement of serum lipid profile including triglycerides (TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), and ratios of cholesterol such as TC:HDL-C, LDL-C:HDL-C, and TG:HDL-C. Classification was carried out according to the different cut-off levels as recommended by the 2001 American National Cholesterol Education Program (NCEP) III using thresholds of TG > or = 150 mg/dl (2.0 mmol/L), LDL-C > or = 100 mg/dl (2.6 mmol/L), HDL-C<40 mg/dl (1.04 mmol/L), and non-HDL-C> or 130 mg/dl (3.37 mmol/L). The criteria of the 1998 British Hyperlipidemia Association (BHA) were also followed using thresholds of TG > or =180 mg/dl (2.4 mmol/L), TC > or 194 mg/dl (5.0 mmol/L), LDL-C > or = 116 mg/dl (3.0 mmol/L), HDL-C< or =45 mg/dl (1.15 mmol/L), TC:HDL-C> or =5.0, HDL-C:DL-C> or =2.5, and TG:HDL-C> or =3.0. RESULTS: Based on the American NCEP III criteria, the dyslipidemic states were noted with high TG (41.6%), high LDL-C (57.8%), low HDL-C (49.9%), and high non-HDL-C (56.8%) from the subjects. Based on the BHA criteria, high TG (24.5%), high TC (32.7%), high LDL-C (37.8%), high TC:HDL-C (30.9%), high LDL-C HDL-C (55.7%), and high TG:HDL-C (58.3%) were noted. CONCLUSION: Dyslipidemia is common in the Iraqi population, and this was identified at different recommendation criteria.