RP-HPLC法测定盐酸利多卡因注射液含量—中国药典(2005版)和美国药典(30版)的方法比较

目的用反相高效液相色谱法（RP-HPLC）测定盐酸利多卡因注射液含量,并将中国药典（2005版）与美国药典（30版）两种方法进行比较。方法用两国药典方法（反相高效液相色谱法）测定盐酸利多卡因注射液含量。中国药典（2005版）色谱条件：C18柱,以磷酸盐缓冲液-乙腈（50∶50）,用磷酸调节PH至8.0为流动相,检测波长254 nm。美国药典（30版）色谱条件：C18柱,以醋酸钠溶液-乙腈（4∶1）为流动相,检测波长254 nm。结果中国药典（2005版）方法盐酸利多卡因在0.1-2.4 g·L^-1浓度范围内,浓度与峰面积线性关系良好,相关系数r=0.9996。美国药典（27版）方法盐酸利多卡因在0.1-2.4g·L^-1浓度范围内,浓度与峰面积线性关系良好,相关r=0.9998。回收率、精密度两者无显著差异。结论两者盐酸利多卡因浓度与峰面积均具有良好的线性关系。     

反相高效液相色谱法测定法莫替丁片含量--中国药典(2000版)和美国药典(26版)方法的比较

目的 用RP-HPLC测定法莫替丁片含量,并将中国药典(2000版)与美国药典(26版)两种方法进行比较.方法 用反相高效液相色谱测定法莫替丁片含量.中国药典(2000版)色谱条件:C18柱,以庚烷磺酸钠溶液(取庚烷磺酸钠2.0 g,加水900ml溶解后,用冰醋酸调节pH至3.9,加水至1000 ml)-乙腈-甲醇(25:6:1)为流动相,检测波长254 nm.美国药典(26版)色谱条件:C18柱,以水-甲醇-磷酸二氢钾缓冲液(31:6:3,用磷酸调节pH至5.0)为流动相,检测波长254 nm.结果 中国药典(2000版)方法法莫替丁在0.012 5～0.20 g·L-1浓度范围内,浓度与峰面积线性关系良好,相关系数r=0.999 9.美国药典(26版)方法法莫替丁在0.002 024～0.202 4 g·L-1浓度范围内,浓度与峰面积线性关系良好,相关系数r=0.999 9.结论 二者法莫替丁浓度与峰面积都具有良好的线性关系,但美国药典(26版)与中国药典方法(2000年版)相比,峰面积较小,但流动相成本较低,易得,更安全.     

HPLC法测定盐酸林可霉素含量——中国药典(2005版)和美国药典(27版)方法的比较

目的用高效液相色谱法(HPLC)测定盐酸林可霉素含量,并将中国药典(2005版)与美国药典(27版)两种方法进行比较。方法用两国药典方法测定盐酸林可霉素含量。中国药典(2005版)色谱条件:C1 8柱,以0.05 mol.L-1硼砂溶液(85%磷酸溶液调节pH值至6.0)-甲醇(4∶6)为流动相,检测波长214 nm,待测溶液浓度2.0 g.L-1;美国药典(27版)色谱条件:C8柱,以磷酸溶液-甲醇-乙腈(780∶150∶150)为流动相,检测波长210 nm,待测溶液浓度1.2 g.L-1。结果中国药典(2005版)方法盐酸林可霉素在浓度范围内,浓度与峰面积线性关系良好,相关系数r=0.9999;美国药典(27版)方法盐酸林可霉素在浓度范围内,浓度与峰面积线性关系良好,相关系数r=0.9997。平均回收率在99.0%~101.0%,RSD均小于0.5%结论二者盐酸林可霉素浓度与峰面积均具有良好的线性关系,含量相差不大,无明显差别。     

HPLC测定茴三硫注射液中盐酸利多卡因的限度

目的 建立测定茴三硫注射液中盐酸利多卡因的方法.方法 采用HPLC法和C18色谱柱(150 mm×4.6 mm,5μm),以甲醇-醋酸钠溶液(65:35)为流动相,流速为0.5 ml·min-1,测定波长为254 nm.结果 盐酸利多卡因26.00～1.04 × 103mg·L-1与峰面积呈良好的线性关系(γ=0.9999);平均回收率为98.36%,RSD=1.2%(n=9).结论 所建方法能有效地分离盐酸利多卡因和主药茴三硫及其相邻峰,空白无干扰,方法简便,结果准确.     

反相高效液相色谱法测定盐酸头孢替安酯含量及有关物质

目的建立反相高效液相色谱法测定盐酸头孢替安酯的含量及有关物质。方法色谱柱为Hypersil C18柱(4.6mm×250mm,5μm),流动相:0.2mol.L-1磷酸二氢钾-乙腈-冰醋酸=72∶28∶1;流速:1mL.min-1,检测波长:254nm,柱温:25℃。结果盐酸头孢替安酯浓度在8～64μg.mL-1时,其峰面积与浓度的线性关系良好(r=0.999 2);平均加样回收率为100.0%,RSD为1.3%(n=6),其有关物质1、2、3的百分含量均低于日本药典15版规定值。结论本法快速、简便、准确、灵敏度高,适用于盐酸头孢替安酯原料药的含量及有关物质的测定。     

高效液相色谱法测定盐酸萘替芬的含量

目的:建立非水反相高效液相色谱法测定盐酸萘替芬的含量.方法:采用Phenomenex C18色谱柱(250 mm×4.6mm,5μm),乙腈为流动相,流速1.0 mL·min-1,检测波长254nm.结果:盐酸萘替芬在9.7～29.1 mg·L-1浓度范围内与峰面积线性关系良好,r=0.999 9.平均回收率为100.3%,RSD＜0.78%.结论:本方法简便,快速,准确,可用于盐酸萘替芬的快速含量测定.     

异硫氰酸苯酯柱前衍生法测定复方氨基酸注射液(3AA)中氨基酸含量

目的:建立采用异硫氰酸苯酯柱前衍生化高效液相色谱法测定复方氨基酸注射液(3AA)的含量。方法:色谱柱:Diamonsil C18(2)(250 mm×4.6 mm,5μm);流动相:A为0.1 mol/L醋酸钠溶液(pH 6.5)-乙腈(93∶7);B为乙腈-水(80∶20)梯度洗脱,流速:1.0 ml/min;柱温:40℃;检测波长:254 nm。结果:各个氨基酸的浓度与其相应色谱峰面积的线性相关系数均达到0.999以上;各个氨基酸的浓度与其相应色谱峰面积呈良好的线性关系,回收率在98.5%～99.6%。结论:该方法适用于测定复方氨基酸注射液(3AA)的含量,具有准确、快速和灵敏度高等优点。     

RP-HPLC测定盐酸奈必洛尔含量和有关物质

目的建立反相高效液相色谱法测定盐酸奈必洛尔含量和有关物质。方法采用的色谱柱为ODS色谱柱(250 mm×4.6 mm,5μm);以甲醇为溶剂,进样浓度:0.5 g.L-1,0.05 mol.L-1的磷酸盐缓冲液(2%三乙胺,pH=3.5)∶乙腈∶甲醇=200∶115∶40为流动相;检测波长为280 nm。结果盐酸奈必洛尔峰与杂质峰分离良好,盐酸奈必洛尔浓度在31.71～158.55 mg.L-1与峰面积呈良好的线性关系(r=0.999 7),最低检检测限为5 ng。方法精密度RSD为0.35%。结论采用反相高效液相色谱法测定盐酸奈必洛尔含量及其有关物质方法快速简便,结果准确。也可用于该品制剂中有关物质和含量的测定。     

HPLC测定注射用盐酸头孢替安含量

目的建立测定注射用盐酸头孢替安含量的反相高效液相色谱法。方法色谱柱为DiamonsilTM C18（4.6 mm×250 mm,5 μm）,流动相为乙腈-水（1.5%乙酸）（5∶95）,流速1.0 mL·min-1,检测波长254 nm,柱温40 ℃。结果盐酸头孢替安浓度在12~2 000 mg·L-1内,峰面积与浓度呈良好线性关系(r=0.999 9),加样回收率为98.8%, RSD为1.35%(n=5)。结论该方法简便可靠,能够满足注射用盐酸头孢替安的含量测定要求。     

反相离子对色谱法测定三利巴布膏剂中盐酸利多卡因的含量

目的 建立反相离子对色谱法测定三利巴布膏荆中盐酸利多卡因的含量.方法色谱柱为Kromasil-C18(150 mm x4.6mm,5μm),流动相为乙腈0.02 mol·L-1磷酸二氢钠-三乙胺(48520.15),用磷酸调节PH至3.15,内含SDS 0.015 mol·L-1;检测波长220 nm;流速1.0 mL·min-1.结果盐酸利多卡因在2.01～20.10μg·mL-1线性关系良好(r=0.999 9,n=5).低、中、高三种浓度的回收率分别为(99.63±0.90)%、(99.55±0.50)%、(99.26±0.26)%.结论 建立的反相离子对色谱法适用于该制剂中盐酸利多卡因的质量控制.     

Validity of the substance abuse subtle screening inventory-adolescent version (SASSI-A)

The construct and convergent validity of the Substance Abuse Subtle Screening Instrument-Adolescent (SASSI-A) were examined in a population of suburban youth offenders. The analysis was conducted on archived data of 490 adolescent offenders from a suburban Circuit Court-Juvenile Division. Exploratory factor analysis results best supported a 5-factor solution that accounted for a modest 32.5% of the total variance. This 5-factor solution had a poor goodness of fit with the purported factor structure upon which the formal SASSI-A scoring is based. The relationship between the SASSI-A and variables with known relationships to adolescent substance abuse (i.e., lack of religious involvement, single parent household, learning disability, early onset of use, conduct problems) was examined. Results revealed moderately strong relationships between the SASSI-A and these variables, indicating reasonable convergent validity. It is concluded that the SASSI-A face valid scales have moderate utility for identifying substance dependence within this sample, while the subtle scales do not.     

Psychometric properties of the Turkish version of the Internet Addiction Test (IAT)

ObjectiveOf many instruments developed to assess Internet addiction, the Internet Addiction Test (IAT), an expanded version of the Internet Addiction Diagnostic Questionnaire (IADQ), has been the most widely used scale in English and non-English speaking populations. In this study, our aim was to investigate the psychometric properties of short and expanded versions of the IAT in a Turkish undergraduate sample.MethodOverall, 455 undergraduate students from Turkey aged between 18 and 30 participated in the study (63.53% were females). Explanatory and confirmatory factor analytic procedures investigated factor structures of the IADQ and IAT. The Internet Addiction Scale (IAS), Coping Inventory for Stressful Situations (CISS), Obsessive Compulsive Inventory—Revised (OCI-R) and Dissociative Experiences Scale (DES) were administered to assess convergent and divergent validities of the IADQ and IAT. Internal consistency and 15-day test–retest reliability were computed.ResultsIn the factorial analytic investigation, we found a unidimensional factor structure for each measure fit the current data best. Significant but weak to moderate correlations of the IADQ and the IAT with the CISS, OCI-R and DES provided empirical evidence for divergent validity, whereas strong associations with the subscales of the IAS pointed to the convergent validity of Young's Internet addiction construct. Internal consistency of the IADQ was weak (α = 0.67) and of the IAT was high (α = 0.93). Temporal reliability of both instruments was very high (α = 0.81 and α = 0.87; respectively).ConclusionThe IAT revealed promising and sound psychometric properties in a Turkish sample.     

Confirmatory factor analysis of the Brazilian version of the Drinking Motives Questionnaire-Revised (DMQ-R)

The main objective of this study was to evaluate the factor structure of the Brazilian version of the Drinking Motives Questionnaire-Revised (DMQ-R; Cooper, 1994) in a sample of 584 university students. A secondary goal was to investigate the relationships between motives and measures of alcohol use and drinking problems. The DMQ-R assesses four motive dimensions: social, enhancement, coping, and conformity. Confirmatory factor analysis supported a revised four-factor model identical to the original, with the exception of one item that did not load on its intended factor. Relative to coping and conformity motives, enhancement and social motives were strongly related to both alcohol use and drinking problems. Overall results indicate that the factor structure of the Brazilian DMQ-R parallels that observed in North America and Europe using the original English language DMQ-R, despite a distinct pattern of relationships with alcohol use and drinking problems.     

Validation of the Dutch version of the brief young adult alcohol consequences questionnaire (B-YAACQ)

Purpose

The purpose of this study was to validate the Dutch version of the Brief Young Adult Alcohol Consequences Questionnaire (B-YAACQ).

Method

The B-YAACQ was translated into the Dutch language and 800 students were invited to complete the questionnaire. A number of drinking behaviors were also assessed; including weekly drinking, age of drinking onset, and blood alcohol concentration on a typical night out. Students also completed a short version of the Alcohol Use Disorders Identification Test (AUDIT-PC).

Results

Data from 667 undergraduate and graduate students (184 men and 483 women) who reported alcohol use during the past year was used in the analysis. On average, students in this study report 4.7 alcohol-related consequences. The Dutch B-YAACQ was shown to have a high reliability and validity: Cronbach's Alpha was 0.816, and B-YAACQ scores correlated significantly with AUDIT-PC scores (r = 0.747). B-YAACQ scores correlated significantly (p < 0.01) with various drinking variables, including drinking onset age, drinking days per week, alcoholic drinks per week, number of drinks on a night out and blood alcohol concentration when returning home.

Conclusion

The Dutch B-YAACQ is a useful new tool for screening of alcohol-related consequences.     

Reliability and validity of the German version of the European Addiction Severity Index (EuropASI)

OBJECTIVE: This study provides data on the psychometric characteristics of the German version of the European Addiction Severity Index (EuropASI). The ASI is a frequently used clinical and research instrument that measures problem severity among people with substance dependence. METHOD: The German ASI was used in a sample of 112 consecutively admitted male psychiatric inpatients seeking treatment for severe alcohol problems. The conceptual structure of the German ASI subscales was investigated by analyzing the intercorrelations of the severity ratings and composite scores. Internal consistency, interrater reliability and concurrent validity in terms of correlations with other assessment instruments were evaluated. RESULTS: The German ASI subscales proved to be independent or moderately correlated (-0.17 < r < 0.34). Each correlation coefficient between corresponding severity ratings and composite scores was significant (p < .0005), ranging from r = 0.47 to r = 0.93. Reliability measures indicated moderate to good internal consistency (Cronbach's alpha: 0.69-0.92) and moderate to excellent interrater reliability (intraclass correlation coefficient: 0.62-0.99). Validity was supported by significantly higher ratings in the alcohol section in alcohol dependent patients compared to patients without dependence (t = 2.99, 108 df, p = .004). Significant correlations (p < .001) were found between the alcohol use section and the Michigan Alcoholism Screening Test (r = 0.34 composite score and r = 0.44 severity rating) and between psychiatric status and the Symptom Checklist-90-revised (r = 0.55/0.51), supporting concurrent validity. CONCLUSIONS: The German version presented evidence of acceptable psychometric properties and its applicability in German-speaking countries could be confirmed.