Clinical Observation Ulcerative Colitis Treated by Acupuncture at Jiaji Points (EX-B2) and Tapping with Plum-blossom Needle at Sanjiaoshu (BL22) and Dachangshu (BL 25)—A Report of 43 Cases

Ulcerative colitis (UC) is an inflammatory diseasewith unknown causes,which involves the rectum andcolon and relates to autoimmunity.As a commonlyencountered,difficult and complicated disease of thedigestive tract,it is clinically characterized bydiarrhea,mucous bloody stool often with pus,abdominal pain,and rectal tenesmus.The majority ofthe patients have a chronic and intermittent course ofdisease,and the severer case may inducecancerization.Since 1999,43 cases of ulcerativecolitis have been treated in this hospital by     

Treatment of Urticaria with Cupping at Back-Shu Points——A Report of 40 Cases

Urticaria is a common and frequentlyencountered disease,which is mainly manifested byextreme pruritus and lumpish eruption of the skin.Acute urticaria with a short disease course can becured,while the chronic one with repeated attacks is     

Pharmacological intervention of hypertension in proteinuric chronic kidney disease： how and what？

Chronic kidney disease （CKD） is a significant interactive disease in patients with diabetes, hypertension, and cardiovascular disease with major morbidity consequences and high costs to the healthcare system. The prevalence of end stage renal disease （ESRD）, a f＇mal outcome of CKD, has increased progressively in the past decades in both developing and developed countries. It is now appreciated that the majority of patients with CKD have a chronic decline of renal function over years before renal replacement therapy is required. This non-specific process continues even when the initial insult is no longer present and has been called progression of CKD.     

Cancer and Thrombosis

Since 1865,Aremand Trousseau first reported on the association between cancer and thrombosis.For many years,it has been recognized that venous thromboembo- lism (VTE) is a common occurrence in patients with ma- lignant disease.Compared to other groups of patients with VTE the cancer population is unique because the pathogenesis of thrombosis differs,the frequency of VTE is greater and the clinical management required is more     

Elevated Plasma Tissue-type Plasminogen Activator （t-PA） and Soluble Thrombomodulin in Patients Suffering From Severe Acute Respiratory Syndrome （SARS） as a Possible Index for Prognosis and Treatment Strategy

INTRODUCTION Severe acute respiratory syndrome(SARS)is a life-threatening infectious disease associated with endothelial injury,coagulation activation,and intravascular fibrin deposition.A recent study of138patients in Hong Kong has shown that44.8%of the patients have thrombocytopenia,45.0%have elevated level of D-dimers,and42.8%have a prolonged activated partial-thromboplastin time[1],suggesting that SARS infection is associated with disseminated intravascular coagulation(DIC)[2-4].…     

Rasmussen’s syndrome and its treatment by hemispherectomy

Rasmussen syndrome is a special disease which mainly affects one side of the cerebral himsphere and is complicated by intractable epilepsy. This disease is seldom encountered. We treated satisfactorily cases of Rasmussen syndrome with modified himspherectomy.     

Aortopulmonary window： a case diagnosed and surgery confirmed by ultrafast computed tomography

Aortopulmonary window (APW) is a rare congenital defect,found in 0.2% of patients with congenital heart disease,in which a communication exists between the ascending aorta and the pulmonary artery trunk with normal separation of the aortic and pulmonary valves.     

Leukocyte Telomere Length and Lacunar Stroke: A Mendelian Randomization Study

<正>Lacunar stroke is a cerebral small vessel disease that accounts for nearly 25% of cases of ischemic stroke. As an age-related disease, the incidence of lacunar stroke increases with age. Generally,biological age is equal to chronological age.However, under the influence of a range of chronic diseases and circumstances, the biological age can accelerate, which may contribute to the variation in risk of illness and death between individuals.     

Recurrent perimedullary arteriovenous fistula at thoracic level

Perimedullary arteriovenous fistula （PMAVF, type Ⅳ spinal cord arteriovenous malformation,SCAVM） is a direct arteriovenous shunt without abnormal vascular connection between the feeding artery and draining vein. Most patients with PMAVF present with a progressive myelopathy caused by venous hypertension, resulting in disabling deficits and incurable complete transverse myelopathy. The lesion is usually located on the surface of the spinal cord or under the pia mater at the level of the conus medullaris or cauda equina, thoracic PMAVF is rarely encountered. Most PMAVFs are fed by the anterior spinal artery （ASA）, posterior spinal artery （PSA）, or both Multiple arterial feeders from the ASA can make the treatment of the disease difficult From August 2004 to February 2005, we treated a patient with a recurrent PMAVF （type Ⅳb） at the thoracic level with multiple blood supply.     

中髎穴古今研究说略

临床上,中髎穴的定位存在一定难度,针刺的角度、深度亦有差异,针刺中髎产生的针感强弱、感传的方向不同会导致治疗的结果不同。目前对于中髎穴的机制研究尚不够完善,多为临床研究,且关于其作用于靶向器官产生调节功能的分子生物学机制有待深入探讨。今后应尽早规范中髎穴的临床操作,制定相应标准,临床取穴结合文献所测定的值以提高精准度;深入探究中髎穴的不同针刺深度对不同疾病效应结果的差异;进一步研究针刺中髎穴的生物学机制;加强针刺中髎的大样本随机双盲对照临床试验。     

温和灸肾俞穴对衰老大鼠血清MDA及肝肾功能的影响

目的通过检测自然衰老大鼠血清MDA及血清ALT、AST、Scr、BUN含量变化,观察不同时序艾灸肾俞穴延缓大鼠衰老的生物学效应及长期艾灸肾俞穴对肝肾功能的影响。方法将40只6月龄雄性清洁级SD大鼠随机分为4组,艾灸1组、艾灸2组、24月龄对照组和18月龄对照组,每组10只,分别饲养至相应月龄后开始艾灸肾俞穴。采用TBA法检测血清中MDA含量;采用ELISA方法检测ALT、AST、Scr、BUN水平。结果与18月龄对照组比较,24月龄对照组大鼠血清MDA、ALT、BUN含量明显升高,差异具有统计学意义(P0.01或P0.05);与24月龄对照组比较,艾灸1组、艾灸2组血清MDA水平均有明显降低(P0.01);血清ALT及AST表达与24月龄对照组比较差异无统计学意义(P0.05);艾灸2组大鼠血清BUN水平降低,差异有统计学意义(P0.05)。结论不同时序艾灸肾俞穴均可降低衰老大鼠血清MDA含量,且对衰老大鼠肝肾功能无损伤作用。     

厥阴俞考源及功用析疑

有关厥阴俞最早的描述见于《素问·长刺节论》,此后唐《千金要方》中四见而四名,同时载有定位、主治,至宋《太平圣惠方》将之归入膀胱经,使之正式成为背俞穴,及至明《针灸聚英》首次明确其为心包络的背俞穴。多数古今文献记载厥阴俞的主治病证病位在心肺、胸背,但厥阴俞对齿痛、膝痛及全身多种疾病均具有一定的治疗作用,而文献中所见较少,试举例分析。     

膏肓灸法对大耳白兔类风湿性关节炎免疫因子的影响

目的观察膏肓灸法对佐剂型类风湿性关节炎（RA）大耳白兔血清IgG、Iga含量及红细胞C3b受体花环形成率（RBC—C3bR）、红细胞复合物花环形成率（RBC—IcR）的影响,为本法在临床应用提供理论依据。方法将40只日本大耳白兔随机分为正常组、RA模型组、药物对照组和膏肓灸法组各10只。除正常组外,其余各组均按文献方法造模。药物对照组每次给予埏痹冲剂0．56g,1日3次;膏肓灸法组每日治疗1次。10d后检测结果。结果膏肓灸法组和药物对照组血清IgG、IgA含量明显降低（P〈0．05）,膏肓灸法组疗效低于药物对照组（P〈0．05）;膏肓灸法组和药物对照组对RBC—C3bR的升高作用明显（P〈0．05）,均能降低RBC—IcR（P〈0．05）,膏肓灸法组疗效低于药物对照组（P〈0．05）。结论膏肓灸法具有较强的改善体液免疫作用,并能提高红细胞免疫功能。     

Effect of acupuncture at acupoint of Shenshu (BL 23) on the bones of ovariectomized rats

OBJECTIVE

To investigate the effect of acupuncture at acupoint of Shenshu (BL 23) in ovariectomized rats and sham-operated rats.

电针“心俞-神门”对急性心肌缺血模型大鼠心肌与海马组织Glu、Asp和NR1表达的影响

目的观察电针“心俞-神门”配穴对急性心肌缺血(acute myocardial ischemia,AMI)模型大鼠心肌和海马组织谷氨酸(Glu)、天冬氨酸(Asp)和N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体亚基NMDAR1(NR1)的影响,探讨俞原配穴的协同效应,及其对AMI致脑损伤保护的作用机制。方法将60只健康雄性SD大鼠随机分为假手术组、模型组、电针“神门”组(神门组)、电针“心俞”组(心俞组)和电针“心俞+神门”组(心神组),每组12只。除假手术组外,其他4组大鼠选用冠状动脉左前降支结扎法建立AMI模型。干预组每天电针治疗1次,20 min/次,共治疗1周,假手术组和模型组不作干预治疗。观察分析心电图ST段变化;ELISA法检测各组大鼠心肌和海马组织中Glu、Asp浓度;实时荧光定量PCR检测各组大鼠心肌和海马组织中NR1 mRNA表达。结果与假手术组比较,模型组大鼠心电图ST段明显抬高(P<0.01),心肌、海马Glu、Asp浓度和NR1 mRNA表达均显著上升(P<0.01);与模型组比较,3组电针组治疗后ST段明显降低(P<0.01),心神组心肌和海马Glu、Asp浓度、NR1 mRNA表达均明显下降(P<0.01),神门组、心俞组心肌和海马NR1 mRNA表达及心肌Asp、海马Glu浓度均显著下降(P<0.01);与神门组比较,心神组心肌Asp浓度和海马Glu、Asp浓度及心肌和海马的NR1 mRNA表达均明显下降(P<0.05或P<0.01);与心俞组比较,心神组心肌和海马Asp浓度、NR1 mRNA表达明显下降(P<0.05或P<0.01)。Pearson相关分析显示,电针治疗后心肌、海马组织中Glu浓度与NR1 mRNA表达呈正相关。结论电针俞原配穴“心俞-神门”能够改善AMI模型大鼠心肌缺血状态,降低心肌组织和海马组织的兴奋性氨基酸Glu、Asp和NR1水平,可能是其发挥保护心肌缺血致脑损伤的作用机制之一。     

天麻素对百草枯和代森锰诱导的小鼠脑黑质多巴胺能神经元损伤的保护作用

目的：观察天麻素（GAS）对百草枯（PQ）和代森锰（MB）致小鼠黑质多巴胺（DA）能神经元损伤的神经保护作用及其机制。方法：用PQ和MB建立C57BL小鼠PD模型,设立空白组、模型组、GAS低剂量组、GAS中剂量组、GAS高剂量组5组,每组15只,在应用PQ及MB后给予GAS干预,观察小鼠行为学改变,进行TH免疫组织化学染色,并测定黑质区MDA、GSH-Px、GSH、SOD的含量。结果：小鼠注射PQ和MB后出现震颤、竖尾、竖毛、运动迟缓、步态不稳、肢体僵硬等症状。同时黑质致密部（SNc）的TH阳性细胞明显减少;GAS低、中、高剂量组症状较模型组轻,TH阳性细胞数均较模型组明显增加（P〈0.05）。GAS低、中、高剂量组MDA含量（分别为6.5 nmol/mg±2.7 nmol/mg、6.2 nmol/mg±2.3 nmol/mg、5.3 nmol/mg±1.6 nmol/mg）与模型组MDA含量为9.7 nmol/mg±3.1 nmol/mg相比明显降低（P〈0.05）,GAS低、中、高剂量组GSH-Px、GSH、SOD的含量（GSH-Px的含量分别为3.1nU/g±0.3 nU/g、3.5 nU/g±0.5 nU/g、3.9 nU/g±0.4 nU/g;GSH的含量分别为6.1 mg/g±2.1 mg/g、6.5 mg/g±1.9 mg/g、6.8 mg/g±1.7 mg/g;SOD的含量分别为160.3 nU/mg±7.3 nU/mg、164.5 nU/mg±10.2 nU/mg、182.1 nU/mg±9.7 nU/mg）与模型组GSH-Px、GSH、SOD的含量（2.5 nU/g±0.4 nU/g、4.4mg/g±1.5 mg/g、138.5 nU/mg±6.4 nU/mg）相比明显增高（P〈0.05）。结论：GAS对PQ和MB诱导的DA能神经元损伤具有一定的保护作用,其机制可能与清除过氧化脂质及自由基,提高机体抗氧化能力有关。     

艰难梭菌感染C57BL/6小鼠模型的建立方法研究

目的 建立C57BL/6小鼠抗菌药物诱导艰难梭菌感染模型,为更有效地认识人类艰难梭菌感染性疾病的发病机制及治疗措施提供依据。方法 C57BL/6小鼠经抗菌药物混合液预处理3天后,进行克林霉素腹腔注射。之后给予高、中、低浓度艰难梭菌ATCC43255悬液灌胃,建立艰难梭菌感染诱导肠损伤和结肠炎的CDI模型。观察小鼠的病死率,平均相对体重、结肠组织病理学评分和粪便艰难梭菌A&B毒素变化。结果 小鼠经抗菌药物处理和艰难梭菌灌胃后,出现腹泻,体重下降,粪便艰难AB毒素检测阳性。结肠病理切片可见炎性细胞浸润和组织坏死等结肠炎表现。随着灌胃菌液浓度的增加,小鼠症状越重,粪便毒素值越高。造模后存活下来的小鼠即使再次给予艰难梭菌灌胃也不再出现症状。结论 C57BL/6小鼠艰难梭菌感染模型与人类CDI病程变化相似,可进一步用于CDI防治措施的研究。     

电针肾俞、膈俞、百会穴对拟血管性痴呆学习记忆障碍小鼠水迷宫实验的影响

目的　观察电针肾俞、膈俞、百会穴对拟血管性痴呆小鼠学习记忆障碍的影响。方法　复制脑缺血再灌注拟血管性痴呆小鼠模型 ,并电针肾俞、膈俞、百会穴 ,分别于术后 7天、15天、30天 ,与药物喜德镇对照 ,采用水迷宫法 ,记录游全程时间、错误次数。结果　造模导致了小鼠学习与记忆能力下降 ,表现为游全程时间延长 ,错误次数增加 ,且随着观察时间的延长 ,这种改变逐渐加重。电针和喜德镇均可使模型小鼠的游全程时间缩短 ,错误次数减少 ,但电针组于 7天和 30天时 ,成绩显著优于药物组。结论　电针肾俞、膈俞、百会穴对模型小鼠学习与记忆能力下降有改善和提高作用