流苏龙胆中的酮类成分研究

目的研究流苏龙胆Gentiana panthaica中的酮类成分。方法采用多次的硅胶柱色谱法分离纯化,并通过理化性质和光谱与波谱分析技术鉴定其化学结构。结果从流苏龙胆中分离得到6个酮类化合物,分别鉴定为:1-羟基-2,3,4,7-四甲氧基酮(Ⅰ)、1,8-二羟基-3,5-二甲氧基酮(Ⅱ)、1-羟基-2,3,4,5-四甲氧基酮(Ⅲ)、1-羟基-2,3,7-三甲氧基酮(Ⅳ)、1-羟基-2,3,5-三甲氧基酮(Ⅴ)和1,7-二羟基-2,3,5-三甲氧基酮(Ⅵ)。结论6个化合物均为首次从该植物中分离得到。     

藏药花锚中的(口山)酮类成分及其抗氧化活性

目的研究藏药椭叶花锚中(口山)酮类成分及其抗氧化活性.方法利用正相硅胶柱色谱、Sephadex LH-20柱色谱、反相高效液相色谱和重结晶等手段进行分离;借助红外光谱、质谱及核磁共振谱等波谱方法进行结构鉴定;以不同浓度化合物对Fe2 -半胱氨酸(Cys)诱导大鼠肝微粒体丙二醛生成的影响评价其抗氧化活性.结果分离得到8个(口山)酮化合物(化合物Ⅰ～Ⅷ),其结构鉴定为1,7-二羟基-2,3,5-三甲氧基(口山)酮、1-羟基-2,3,4,7-四甲氧基(口山)酮、1,7-二羟基-2,3,4,5-四甲氧基(口山)酮、1,7-二羟基-2,3-二甲氧基(口山)酮、1,5-二羟基-2,3-二甲氧基(口山)酮、1-羟基-2,3,5-三甲氧基(口山)酮、1-羟基-2,3,4,5-四甲氧基(口山)酮、1-羟基-2,3,5,7-四甲氧基(口山)酮;其中1,7-二羟基-2,3,5-三甲氧基(口山)酮为新化合物;化合物Ⅰ、Ⅲ(10μg/ml、100 xg/ml)对Fe2 -Cys诱导大鼠肝微粒体丙二醛生成有显著抑制作用.结论从椭叶花锚乙醇提取物乙酸乙酯萃取部分分离得到8个(口山)酮化合物,且该类化合物具有一定抗氧化活性.     

藏药大籽獐牙菜的化学成分研究

目的研究藏药大籽獐牙菜Swertia macrosperma C.B.Clark全草的化学成分。方法运用硅胶柱层析和高效液相色谱法进行化学成分的分离纯化,通过理化常数和波谱分析鉴定化合物结构。结果从大籽獐牙菜的80%乙醇提取物中分离得到9个化合物,分别鉴定为芒果苷(Ⅰ)、1-羟基-3,7,8-三甲氧基口山酮(Ⅱ)、1,8-二羟基-3,7-二甲氧基口山酮(Ⅲ)、1,7-二羟基-3,8-二甲氧基口山酮(Ⅳ)、1,5,8-三羟基-3-甲氧基口山酮(Ⅴ)、1,7,8-三羟基-3-甲氧基口山酮(Ⅵ)、獐牙菜苦苷(Ⅶ)、龙胆苦苷(Ⅷ)、獐牙菜苷(Ⅸ)。结论上述化合物Ⅳ、Ⅵ～Ⅸ为首次从大籽獐牙菜中分离得到。     

川西獐芽菜化学成分研究

目的 研究川西獐牙菜Swertia mussotii的化学成分。方法 采用柱色谱方法进行分离纯化,根据理化性质和波谱解析鉴定化合物结构。结果 从川西獐牙菜75%乙醇提取物中分离得到19个化合物,分别鉴定为1-羟基-3, 4, 7, 8-四甲氧基呫吨酮（1）、1, 7-二羟基-3-甲氧基呫吨酮（2）、1, 3, 7-三羟基呫吨酮（3）、1, 3, 7, 8-四羟基呫吨酮（4）、1, 3, 8-三羟基-7-甲氧基呫吨酮（5）、1, 3-二羟基-7, 8-二甲氧基呫吨酮（6）、1, 5, 8-三羟基-3, 4-二甲氧基呫吨酮（7）、1-羟基-3, 4, 5, 8-四甲氧基呫吨酮（8）、1-羟基-3, 5, 8-三甲氧基呫吨酮（9）、(S)-(+)-龙胆内酯（10a）、(R)-(?)-龙胆内酯（10b）、1, 8-二羟基-3, 7-二甲氧基呫吨酮（11）、1-羟基-3, 7, 8-三甲氧基呫吨酮（12）、1, 7-二羟基-3, 8-二甲氧基呫吨酮（13）、1, 7, 8-三羟基-3-甲氧基呫吨酮（14）、1, 3, 5, 8-四羟基呫吨酮（15）、1, 7-二羟基-3, 4, 8-三甲氧基呫吨酮（16）,芒果苷（17）、齐墩果酸（18）。结论 化合物1～9 均为首次从该种植物中分离得到,化合物10a和10b为首次从獐牙菜属植物中分离得到。     

药用真菌竹黄化学成分的研究

目的 研究药用真菌竹黄Shiraia bambusicola的化学成分。方法 利用硅胶柱色谱及高效制备液相色谱等分离纯化手段，并通过1H-NMR、13C-NMR、MS等波谱学技术进行结构鉴定。结果 共分离得到9个化合物，分别鉴定为3，6，8-三羟基-1-甲基口山酮(1)、3，8-二羟基-6-甲氧基-1-甲基口山酮(2)、2，3，6，8-四羟基-1-甲基口山酮(3)、3，4，6，8-四羟基-1-甲基口山酮(4)、Δ5，10-3β，17α，20β-孕甾三醇(5)、macrosphelide A(6)、(+)-griseofulvin(7)、griseophenone A(8)和抗肿瘤活性成分11，11′-dideoxyverticillin A(9)。结论 化合物1～9均为首次从该真菌中分离得到。     

黄花獐牙菜的化学成分研究

目的 研究黄花獐牙菜 Swertia kingii的化学成分。方法 用各种色谱柱方法进行分离纯化,根据理化性质和波谱解析鉴定其化学结构。结果 从黄花獐牙菜中分离到15个化合物,鉴定了其中的11个化合物,分别为β-谷甾醇(β-sitosterol,1)、1,8-二羟基-3,7-二甲氧基口山酮(甲基獐牙菜宁,methylswertianin,2)、1,7-二羟基-3 ,8二甲氧基口山酮(gentiacaulein,3)、1,8-二羟基-3,5-二甲氧基口山酮(印度獐牙菜素,swerchirin,4)、8-O-β-D-glucopyranosyl-1, 3, 7-trihydroxanthone,5)、芒果苷(mangiferin,7)、1-O-[β-D-吡喃木糖(1→6)-β-D-吡喃葡萄糖］-7-羟基-3,8-二甲氧基口山酮(1-O-［β-D-xylopyranosyl-(1→6)-β-D-glucopyranosyl］-7-hydroxy-3, 8-dimethoxyxanthone,8)、8-O-［β-D-吡喃木糖(1→6)-D-吡喃葡萄糖］-1,7-二羟基-3-甲氧基口山酮(8-O-［β-D-xylopyranosyl(1→6)-lucopyranosyl］1, 7-dihydroxy-3-methoxy-xanthone,9)、1-O-β-D-吡喃葡萄糖-7-羟基-3,8-二甲氧基口山酮(1-O-β-D-glucopyranosyl-7-hydroxy-3,8-dimethoxyxanthone,10)、异荭草苷(isoorientin,11)、8-O-β-D-吡喃葡萄糖-1,3,5-三羟基口山酮(8-O-β-D-glucopyranosyl-1, 3, 5-trihydroxanthone,13)。其余4个还在结构鉴定中。结论 所有化合物1～11均为首次从该植物中得到。     

大籽獐牙菜化学成分研究

目的 研究藏药大籽獐牙菜(Swertia macrosperma C.B.Clark)的化学成分.方法 采用硅胶柱色谱进行分离纯化,根据化合物的理化性质和波谱解析鉴定其化学结构.结果 从大籽獐牙菜的脂溶性部分分离得到6个化合物,分别鉴定为1,8-二羟基-3,5-二甲氧基(口山)酮(swerchirin,Ⅰ)、1-羟基-3,5-二甲氧基(口山)酮(Ⅱ)、1,8-二羟基-3,7-二甲氧基(口山)酮(methylswertianin,Ⅲ)、1-羟基-3,7,8-三甲氧基(口山)酮(decussatin,Ⅳ),齐墩果酸(oleanolic acid,Ⅴ)和1,5,8-三羟基-3-甲氧基(口山)酮(bellidifodin,Ⅵ).结论 化合物Ⅰ～Ⅴ均为首次从该植物中分离得到.     

祁连獐牙菜化学成分研究

目的 研究祁连獐牙菜Swertia przewalskii全草的化学成分。方法 采用硅胶柱层析进行分离纯化,通过波谱方法及化学关联进行结构鉴定;结果 分离纯化出15个化合物,分别鉴定为：1,7-二羟基-3,8-二甲氧基San酮（Ⅰ）,1,8-二羟基-3,7-二甲氧基San酮（Ⅱ）,1,7,8-三羟基-3-甲氧基San酮（Ⅲ）,1,3,7,8-四羟基San酮（Ⅳ）,1-O-β-D-吡喃葡萄糖基-7-羟基-3,8-二甲氧基San酮（Ⅴ）,1-O-β-D-吡喃葡萄糖基-8-羟基-3,7-二甲氧基San酮（Ⅵ）,7-O-β-D-吡喃葡萄糖基-1,8-二羟基-3-甲氧基San酮（Ⅶ）,1-O-[β-D-吡喃木糖（1→6)-β-D-吡喃葡萄糖]-7-羟基-3,8-二甲氧基San酮（Ⅷ）,1-O-[β-D-吡喃葡萄糖]-1,7-二羟基-3-甲氧基S sgmk(X),木犀草素（Ⅺ）,齐墩果酸（Ⅻ）,乌苏酸（XⅣ）和龙胆苦苷（XV）。结论 化合物Ⅳ-Ⅷ,Ⅺ,Ⅻ,XⅣ和XV均为首次从该种植物中分得。     

龙血竭中黄烷和高异黄烷类化学成分研究

研究龙血竭二氯甲烷提取部位中黄烷和高异黄烷类成分。方法 利用硅胶、聚酰胺柱色谱及反复重结晶等方法进行分离纯化,根据理化性质和波谱分析对分离得到的化合物进行结构鉴定。结果 分离得到6个化合物,分别鉴定为7-羟基-4′-甲氧基黄烷（1）、4′-羟基-7-甲氧基-8-甲基黄烷（2）、5,4′-二羟基-7-甲氧基-6-甲基黄烷（3）、7,4′-二羟基高异黄烷（4）、6,4′-二羟基-7-甲氧基高异黄烷（5）和10-羟基-11-甲氧基龙血酮（6）。结论 化合物6为首次从剑叶龙血树中分离得到,化合物2为首次从龙血竭中分离得到。     

青叶胆Shan酮类化合物的成分研究

目的　分离鉴定青叶胆 Swertia mileensis全草的酮类成分。方法　 95 %乙醇浸提 ,经硅胶柱色谱分离纯化 ,UV,IR,MS,1 H和 1 3CNMR波谱方法确定化学结构。结果　分得 12个酮成分 ,分别为 1-羟基 - 2 ,3,4 ,5 -四甲氧基酮 (1- hydroxy- 2 ,3,4 ,5 - tetramethoxyxanthone, ) ;1-羟基 - 2 ,3,7-三甲氧基酮 (1- hydroxy- 2 ,3,7-trim ethoxyxanthone, ) ;1-羟基 - 2 ,3,5 ,7-四甲氧基酮 (1- hydroxy- 2 ,3,5 ,7- tetram ethoxyxanthone, ) ;1,5 -二羟基 - 2 ,3-二甲氧基酮 (1,5 - dihydroxy- 2 ,3- dimethoxyxanthone, ) ;1,5 -二羟基 - 2 ,3,7-三甲氧基酮 (1,5 - dihy-droxy- 2 ,3,7- trimethoxyxanthone, ) ;1-羟基 - 2 ,3,5 -三甲氧基酮 (1- hydroxy- 2 ,3,5 - trimethoxyxanthone, ) ;1,5 -二羟基 - 2 ,3,4 ,7-四甲氧基酮 (1,5 - dihydroxy- 2 ,3,4 ,7- tetramethoxyxanthone, ) ;1,8-二羟基 - 2 ,3,6 -三甲氧基酮 (1,8- dihydroxy- 2 ,3,6 - trimethoxyxanthone, ) ;1-羟基 - 2 ,3,4 ,7-四甲氧基酮 (1- hydroxy- 2 ,3,4 ,7- te-tramethoxyxanthone, ) ;1,2 ,3,5 -四甲氧基酮 (1,2 ,3,5 - tetromethoxyxanthone, ) ;1-羟基 - 2 ,3,4 ,6 -四甲氧基酮 (1- hydroxyl- 2 ,3,4 ,6     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.