Collagen metabolism in experimental lung silicosis. A trimodal behavior of collagenolysis

In spite of several studies, both in vivo and in vitro, the pathogenesis of silicosis remains unclear, mainly in those mechanisms related to fibrogenesis. In this study, we analyzed the concentration, biosynthesis, and degradation of collagen in silica-treated rats 7, 15, 30, 45, and 60 days after instillation. Our results showed a significant increase in collagen content and biosynthesis from the 15th day onward. However, our most remarkable finding was related to collagenolytic activity. In this sense, the silicotic rats presented a trimodal behavior: some animals showed an increased degradation, others had similar values to those of the controls, and others exhibited a decrease of collagenolytic activity. Altogether, these results suggest that collagen deposition in silicotic lungs is due to a rise in biosynthesis and, at least in some animals, to a decrease in degradation. Nevertheless, the steps of collagenolysis must be studied in more detail.     

Effect of aluminum inhalation on alveolar phospholipid profiles in experimental silicosis

In the sheep tracheal lobe model of silicosis, we have recently reported that total phospholipid, lecithin, and phosphatidylglycerol levels were elevated in lung lavage. To investigate further this observation, we obtained complete phospholipid profiles of lung lavage in 10 sheep exposed to saline only (Sa group), 10 sheep exposed to aluminum lactate inhalation only (Al group), 10 sheep exposed to 100 mg Minusil-5 in saline followed by monthly saline inhalation (Si group), and 10 sheep exposed to 100 mg Minusil-5 in saline followed by monthly aluminum lactate inhalation (Si-Al group). The following phospholipid components were measured: total phospholipids, phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine, disaturated phosphatidylcholine, sphingomyelin, and lyso-phosphatidylcholine. All values were comparable in the Sa group, Al group, and Si-Al group. In the Si group, there was a significant increase in total phospholipid to approximately 200% of the control values. The phospholipid profile of this group demonstrated an increase in all of the phospholipid components with some enrichment of the fraction of PG, PE, and PI. We concluded that lung exposure to silica dust significantly increases the concentration of phospholipids in the alveoli. This increase is of a large spectrum of alveolar phospholipids and is completely suppressed by aluminum lactate inhalation.     

矽肺患者肺功能障碍的临床观察

目的探讨矽肺病人各期肺功能通气障碍程度与性质。方法随机选取矽肺患者215例（其中Ⅰ、Ⅱ、Ⅲ期患者分别为167、40、8例）;非矽肺患者30例,设为对照组。本文对这四组病例的血气分析、以及肺功能检查指标进行统计学分析。结果矽肺病人肺功能障碍早期以阻塞型和混合型通气障碍为主,Ⅱ、Ⅲ期矽肺患者肺功能障碍类型以限制性通气功能障碍为主。结论长期接触高浓度的生产性粉尘对肺功能的损害作用明显,而且损害的程度随着病期的进展而加重。     

安体舒通对血压控制平稳的高血压患者胶原代谢的影响

目的观察安体舒通对已控制血压的高血压患者胶原代谢的影响。方法选取已控制血压的原发性高血压患者45例,随机分为对照组和安体舒通组。对照组继续原降压方案治疗,安体舒通组在原降压治疗方案基础上加用安体舒通20mg/d治疗,观察6月。所有患者均在治疗前、后应用超声心动图测定左心室质量指数(LVMI),应用放免技术测定Ⅰ、Ⅲ型胶原血清代谢性标记Ⅰ型前胶原氨端肽(PⅠNP)、Ⅲ型前胶原(PCⅢ)和Ⅰ型胶原C端绞链肽(ⅠCTP)。结果治疗后两组血压皆有下降趋势,但未达统计学差异,各相同观察时点两组间血压比较亦无差异。安体舒通组治疗后的LVMI明显低于治疗前(P<0.05),治疗后安体舒通组与对照组比较LVMI亦明显降低(P<0.05)。与治疗前比较,安体舒通组治疗后血清的PCⅢ、PⅠNP的浓度皆明显降低(P<0.01),但ⅠCTP浓度无变化(P>0.05);治疗后安体舒通组与对照组比较,血清的PCⅢ、PⅠNP的浓度皆明显降低(P<0.01),但ⅠCTP浓度无变化(P>0.05)。结论安体舒通可以抑制血压控制平稳的高血压患者的胶原合成,但可能对其胶原的降解影响不大。安体舒通的这一作用与降压无关,可能属非压力相关性获益。     

安体舒通对血压控制平稳的高血压患者胶原代谢的影响

目的 观察安体舒通对已控制血压的高血压患者胶原代谢的影响.方法 选取已控制血压的原发性高血压患者45例,随机分为对照组和安体舒通组.对照组继续原降压方案治疗,安体舒通组在原降压治疗方案基础上加用安体舒通20 mg/d治疗,观察6月.所有患者均在治疗前、后应用超声心动图测定左心室质量指数(LVMI),应用放免技术测定Ⅰ、Ⅲ型胶原血清代谢性标记Ⅰ型前胶原氨端肽(PⅠ NP)、Ⅲ型前胶原(PCⅢ)和Ⅰ型胶原C端绞链肽(Ⅰ CTP).结果 治疗后两组血压皆有下降趋势,但未达统计学差异,各相同观察时点两组间血压比较亦无差异.安体舒通组治疗后的LVMI明显低于治疗前(P＜0.05),治疗后安体舒通组与对照组比较LVMI亦明显降低(P＜0.05).与治疗前比较,安体舒通组治疗后血清的PCⅢ、PⅠ NP的浓度皆明显降低(P＜0.01),但Ⅰ CTP浓度无变化(P＞0.05);治疗后安体舒通组与对照组比较,血清的PCⅢ、PⅠ NP的浓度皆明显降低(P＜0.01),但Ⅰ CTP浓度无变化(P＞0.05).结论 安体舒通可以抑制血压控制平稳的高血压患者的胶原合成,但可能对其胶原的降解影响不大.安体舒通的这一作用与降压无关,可能属非压力相关性获益.     

早期矽肺患者血清IL-8及支气管灌洗液IL-6含量的测定及与肺功能相关性研究

目的通过对矽肺患者的血清IL-8及肺泡灌洗液的IL-6进行联合检测,并与肺功能进行相关性研究,探讨其在矽肺发病机制中所发挥的作用,为寻找敏感的矽肺诊疗标记物提供依据。方法选择矽肺患者60例为矽肺组,选取普通肺部病变行纤支镜检查明确为非肿瘤的患者50例为对照组,两组分别进行血清中IL-8水平与肺泡灌洗液(BALF)中IL-6含量以及肺功能的检测。结果矽肺组血清中IL-8水平、肺泡灌洗液中的IL-6含量较对照组明显增高(P0.01),差异有显著性,矽肺组在FEV1(%)、FVC(%)、FEV1/FVC(%)均明显较对照组降低,差异具有统计学意义(P0.05)。矽肺患者血清中IL-8水平与肺泡灌洗液中IL-6含量与肺功能检测均存在负相关(r分别为-0.24,-0.31,P均0.05)。结论矽肺患者血清IL-8和灌洗液IL-6含量不仅可作为矽肺早期生物标志物之一,也可以作为评估纤维化倾向和预后敏感指标,将会为早期评估患者病情提供一定的依据。     

Collagen metabolism in experimental cardiac hypertrophy in the rat and the effect of digitoxin treatment.

It is generally agreed that cardiac hypertrophy is accompanied by the hyperplasia of connective tissue cells. In the present work, collagen metabolism was studied in the heart of nondigitalised and digitalised rats after the constriction of the aorta. The activity of prolyl hydroxylase was maximally increased 2 days after the operation. The incorporation of proline into collagen hydroxyproline increased without any increase in the specific radioactivity of free intracellular proline, the peak labelling of collagen occurring at 4 days. Although the treatment of the rats with digitoxin prevented the development of cardiac hypertrophy and an increase in collagen labelling, an increase in the activity of prolyl hydroxylase was observed. The intracellular free proline pool and its specific radioactivity were significantly lower in digitalised rats as compared with non-digitalised rats. The results indicate that constriction of the aorta is accompanied by an activation of connective tissue cells leading to increased synthesis of collagen. However, digitoxin treatment can prevent the increase in collagen labelling, possibly by inhibiting the amino acid transport, but it is unable to remove the stimulus for hypertrophy.     

Effect of NGM282, an FGF19 analogue,in primary sclerosing cholangitis: A multicenter,randomized, double-blind,placebo-controlled phase II trial

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The influence of the ACE inhibitor lisinopril on the glomerular metabolism of proteolytic enzymes in diabetic rats

Clinical studies indicate a nephro-protective effect in conjunction with the use of ACE inhibitors. This study's aim was to determine whether ACE inhibitors influence the metabolism of glomerular cells in addition to their known hemodynamic effects. Streptozotocin diabetic rats were treated with lisinopril (DLis 1.5 mg/l water), or hydralazine (Dhyd, 50 mg/l water) over 4 weeks. Untreated diabetic rats (DC) and non-diabetic rats (C) served as controls. After four weeks of treatment, urinary excretion of albumin, blood pressure and metabolic control (Glyc-Hb) were measured. After treatment glomeruli were isolated and homogenized, and β-NAG and total proteolytic activity against azocasein were measured. Glycated hemoglobin levels were similar in all diabetic groups (DC, 12%, Dhyd, 10%; DLis 11%). Blood pressure of DLis rats (79 ± 3 mmHg) and DHyd rats (46 ± 2 mmHg) was lower than that of DC rats (111 ± 3 mmHg). Urinary albumin excretion of diabetic groups was lowest in DLis. Diabetic rats showed a decrease in glomerular β-NAG (10 vs. 60.5 U/g protein) and total proteolytic activity against azocasein (148 vs. 170 U/mg protein hour) compared to non-diabetic rats. Lisinopril increased β-NAG (30 vs. 14 U/g protein) and total proteolytic activity (160.5 vs. 141.5 U/mg protein hour) compared with hydralazine. Our study confirms that the nephro-protective effect of ACE inhibitors is partially due to modulatory effects on the metabolism of basement membrane proteins. Received: 2 February 2000 / Accepted in revised form: 2 March 2001     

Paraileostomy Recontouring by Collagen Sealant Injection: A Novel Approach to One Aspect of Ileostomy Morbidity. Report of a Case

Introduction Poorly fitting stoma appliances, resulting in stomal leakage and subsequent skin excoriation, remain a significant cause of ileostomy-related morbidity. One cause of ill-fitting stoma bags is the presence of parastomal dermal contour defects/irregularities. These may occur after surgical complications or change in patient weight and body habitus. Methods We report the case of a 29-year-old man who, after panproctocolectomy and formation of ileostomy for ulcerative colitis, experienced significant problems with stoma bag application because of dermal contour defects. As a result, he suffered from significant stomal leakage and skin excoriation. After a single treatment of cutaneous parastomal infiltration of porcine collagen (Permacol™ Injection), applied stoma bags achieved a watertight seal, and the patient experienced complete and sustained resolution of his symptoms. Conclusions Porcine collagen is a safe, versatile, and relatively easy method of restoring irregular skin defects surrounding abdominal stomas, thus resolving the significant patient morbidity associated with ill-fitting stomal appliances. Such a technique avoids the need for surgical stoma refashioning, which may be associated with significant morbidity and unsatisfactory outcomes.     

Matrix metalloproteinases 2, 9, and 13, and tissue inhibitors of metalloproteinases 1 and 2 in experimental lung silicosis.

Exposure to silica induces granulomatous lung inflammation evolving to fibrosis through yet unclear pathogenic mechanisms. We examined the expression of extracellular matrix remodeling molecules: collagenase 3, gelatinases A and B, and TIMP-1 and TIMP-2 in experimental lung silicosis. Rats were instilled with 50 mg of silica and sacrificed after 15 and 60 d. At 60 d a significant increase in lung collagen content was found (170.2 +/- 34.4 versus 88.2 +/- 20.8 microgram/mg in controls, p = 0.01). Gelatin zymography of bronchoalveolar lavage fluid (BALF) from 15 and 60 d revealed bands of progelatinase A and progelatinase B, and lung tissue zymograms showed in addition, the active gelatinase A form at 15 d. By in situ hybridization and immunohistochemistry, early silicotic granulomas exhibited intense staining for all matrix metalloproteinases (MMPs) and TIMPs assayed. Labeling was restricted inside granulomas and surrounding areas. Late silicotic granulomas at 60 d showed lower MMP expression than did early lesions, and in highly fibrotic nodules scarce signal was usually found. TIMP-1 and TIMP-2 showed a moderate reduction in 60-d silicotic nodules. These findings suggest that an imbalance in the expression of MMPs and TIMPs may be implicated in extracellular matrix remodeling and basement membrane disruption during experimental lung silicosis.     

碘过量对实验性自身免疫性甲状腺炎大鼠骨代谢的影响

目的 探讨碘过量对实验性自身免疫性甲状腺炎(EAT)大鼠骨代谢的影响.方法 雌性Lewis大鼠36只,体质量为(131±15)g,按体质量随机分为3组:对照组、EAT组和EAT+高碘组,每组12只.以不同含碘量(0.9、0.9、18.0 mg/kg)的饲料喂养各组大鼠,并用猪甲状腺球蛋白(pTG)和完全弗氏佐剂(CFA)对EAT组和EAT+高碘组大鼠进行免疫以建立EAT模型.2周后观察大鼠甲状腺病理改变,测定血清甲状腺自身抗体[甲状腺球蛋白抗体(TGAb)、甲状腺微粒体抗体(TMAb)]和甲状腺激素[三碘甲腺原氨酸(T3)、甲状腺素(T4)]以及骨代谢相关指标[骨钙素(BGP)、抗酒石酸盐酸性磷酸酶(TRAP)、Ⅰ型前胶原羧基末端前肽(PICP)、Ⅰ型胶原羧基吡啶并啉交联肽(ICTP)、胰岛素样生长因子-1(IGF-1)、护骨素(OPG)、核因子κB受体活化因子配体(RANKL)]水平.结果 EAT组和EAT+高碘组大鼠甲状腺组织均呈现炎细胞浸润,局部滤泡结构破坏,其中EAT+高碘组以甲状腺滤泡扩张、融合为主.EAT组和EAT+高碘组大鼠血清TGAb、TMAb、T3和T4水平[(63.01±12.36)%、(60.62±11.24)%,(3.78±1.43)、(125.12±16.00)pmol/L和(75.00±15.44)%、(72.15±15.00)%,(3.69±0.91)、(149.40±20.67)pmol/L]高于对照组[(4.47±1.04)%、(5.73±1.01)%,(0.75±0.12)、(76.91±9.30)pmol/L,P均＜0.05],EAT+高碘组大鼠血清TGAb、TMAb和T4水平较EAT组升高(P均＜0.05).EAT组大鼠血清BGP、PICP和IGF-1水平[(1.70±0.31)、(11.31±1.52)μg/L,(0.31±0.06)mg/L]较对照组[(8.60±0.33)、(14.28±3.10)μg/L,(1.16±0.02)mg/L]降低(P均＜0.05),血清TRAP、ICTP、OPG和RANKL水平[(19.88±3.60)ng/L,(2.43±0.82)、(22.36±2.80)、(1.35±0.23)μg/L]较对照组[(14.57±3.56)ng/L,(0.50±0.20)、(1.61±0.34)、(0.10±0.02)μg/L]升高(P均＜0.05);与EAT组比较,EAT+高碘组大鼠血清PICP和OPG水平[(8.03±1.84)、(16.80±3.79)μg/L]明显降低(P均＜0.05).结论 EAT时大鼠的破骨细胞分化与成熟增强,导致骨吸收增强.碘过量可抑制EAT大鼠成骨细胞和破骨细胞活性,呈现低转化型骨质疏松.

Abstract：

Objective To explore the effect of iodine excess on bone metabolism in experimental autoimmune thyroiditis (EAT) rats. Methods We selected 36 female Lewis rats with body weight of (131 ± 15)g,and divided them into 3 groups randomly: control group, EAT group and EAT + high iodine group, assuring 12 rats in every group. These rats were fed fodder with different concentration of iodine(0.9,0.9, 18.0 mg/kg), and rats in EAT group and EAT + high iodine group were immunized with pig thyroglobulin(pTG) and complete Freund's adjuvant(CFA) to create EAT model. After two weeks, the pathological changes of the thyroid tissues were observed,and the serum thyroid autoantibody[thyroglobulin antibody(TGAb) and thyroid microsomal antibody(TMAb)], the thyroid hormone levels[triiodo thyronine(T3) and thyrine(T4)] and some relevant data of bone metabolism[bone gla protein (BGP), tartrate-resistant acid phosphatase (TRAP), C-terminal propeptide of type Ⅰ procollagen (PICP),C-terminal telopeptide of type Ⅰ collagen (ICTP), insulin-like growth factor- 1 ( IGF- 1 ), osteoprotegerin (OPG) and receptor activator of NF-κB ligand (RANKL)] were measured. Results Inflammatory cell infiltration and local follicular structural damage were observed in the thyroid tissues of EAT rats in EAT group and EAT + high iodine group, and the pathological changes of EAT + high iodine group were mainly thyroid follicular expansion and integration. The level of serum TGAb, TMAb, T3 and T4 of EAT rats in EAT group and EAT + high iodine group[ (63.01 ± 12.36)%, (60.62 ± 11.24)%, (3.78 ± 1.43), (125.12 ± 16.00)pmol/L and (75.00 ± 15.44)%,(72.15 ± 15.00)%, (3.69 ± 0.91 ), (149.40 ± 20.67)pmol/L] were higher than those of the control group[ (4.47 ±1.04)%, (5.73 ± 1.01 )%, (0.75 ± 0.12), (76.91 ± 9.30)pmol/L, all P ＜ 0.05], and the level of serum TGAb,TMAb and T4 of EAT rats in EAT + high iodine group were higher than those of the EAT group(all P ＜ 0.05).The level of serum BGP, PICP and IGF- 1 in EAT group[ ( 1.70 ± 0.31 ), ( 11.31 ± 1.52) μg/L, (0.31 ± 0.06 ) mg/L]were lower than those of the control group[ (8.60 ± 0.33), (14.28 ± 3.10)μg/L, (1.16 ± 0.02)mg/L, all P ＜0.05], and the level of serum TRAP, ICTP, OPG and RANKL[ ( 19.88 ± 3.60)ng/L, (2.43 ± 0.82), (22.36 ± 2.80),( 1.35 ± 0.23 )μg/L] were higher than those of the control group[ ( 14.57 ± 3.56)ng/L, (0.50 ± 0.20), (1.61 ± 0.34),(0.10 ± 0.02)μg/L, all P ＜ 0.05]; compared with EAT group, the level of PCIP and OPG in EAT + high iodine group [ (8.03 ± 1.84), ( 16.80 ± 3.79)μg/L] were obviously decreased(all P ＜ 0.05). Conclusions The reinforcement of differentiation and maturation of osteoblast in the EAT rats results in the increasing of bone resorption. The activity of osteoblast and osteoclast of the EAT rats are inhibited by excessive iodine, showing a low conversion-type osteoporosis.     

Amelioration of hemodynamics and oxygen metabolism by continuous venovenous hemofiltration in experimental porcine pancreatitis

AIM: To investigate the potential role of continuous venovenous hemofiltration (CVVH) in hemodynamics and oxygen metabolism in pigs with severe acute pancreatitis (SAP). METHODS: SAP model was produced by intraductal injection of sodium taurocholate [4%, 1 mL/kg body weight (BW)] and trypsin (2 U/kg BW). Animals were allocated either to untreated controls as group 1 or to one of two treatment groups as group 2 receiving a low-volume CVVH [20 mL/(kg·h)], and group 3 receiving a high-volume CVVH [100 (mL/kg·h)]. Swan-Ganz catheter was inserted during the operation. Heart rate, arterial blood pressure, cardiac output, mean pulmonary arterial pressure, pulmonary arterial wedge pressure, central venous pressure, systemic vascular resistance, oxygen delivery, oxygen consumption, oxygen extraction ratio, as well as survival of pigs were evaluated in the study. RESULTS: Survival time was significantly prolonged by low-volume and high-volume CVVHs, which was more pronounced in the latter. High-volume CVVH was significantly superior compared with less intensive treatment modalities (low-volume CVVH) in systemic inflammatory reaction protection. The major hemodynamic finding was that pancreatitis-induced hypotension was significantly attenuated by intensive CVVH (87.4±12.5 kPa vs116.3±7.8 kPa,P＜0.01). The development of hyperdynamic circulatory failure was simultaneously attenuated, as reflected by a limited increase in cardiac output, an attenuated decrease in systemic vascular resistance and an elevation in oxygen extraction ratio. CONCLUSION: CVVH blunts the pancreatitis-induced cardiovascular response and increases tissue oxygen extraction. The high-volume CVVH is distinctly superior in preventing sepsis-related hemodynamic impairment.     

Influence of aβ-adrenergic agonist on septic shock-induced alterations of phosphatidylcholine metabolism in rat lung

Changes in surfactant function play an important part in the pathogenesis of adult respiratory distress syndrome (ARDS). Sinceβ-adrenergic agonists have been shown to exert a decisive influence on surfactant secretion, we studied the effect of fenoterol on lung phospholipid metabolism under conditions of experimental sepsis. Fenoterol administered to live rats increased the incorporation of choline into lung tissue by 80% in normal, by 35% in septic animals. It had no comparable effect on palmitate incorporation. It increased the activity of choline kinase in control animals, but had no additional effect on animals with increased values due to sepsis. Phosphotransferase activity diminished during sepsis was stimulated, and phospholipase activity reduced. Fenoterol restored phosphatidylcholine to normal levels in lung tissue and bronchoalveolar lavage and prevented lysophosphatidylcholine generation. Fenoterol also increased the amount of palmitate in phosphatidylcholine from bronchoalveolar lavage in septic animals. The results imply that aβ-adrenergic agonist influences the conditions of lung phospholipid metabolism altered by sepsis towards normal.     

Effect of extended cold ischemia time on glucose metabolism in liver grafts: experimental study in pigs

Background/Purpose

Recovery of normal carbohydrate metabolism in the liver after transplantation is highly important. The aim of the present study was to evaluate how short and long cold ischemia (CI) time followed by warm ischemia (WI) impact intrahepatic glucose metabolism in a pig liver transplantation model.

Methods

Twenty-six animals were divided into two transplantation groups: group I with a liver ischemia time of 5?h (n = 6), and group II with 15?h of liver ischemia (n = 7). Intrahepatic microdialysis samples were collected throughout the experiment at 20-min intervals, during the donor operation, cold preservation, liver implantation, and liver reperfusion in the recipient. Glucose, lactate, and pyruvate concentrations were analyzed and the lactate/pyruvate ratio (L/Pr) was calculated.

Result

There were no changes in glucose levels during CI. However, during WI, glucose and lactate increased and the increase was significantly higher in the group with longer CI (P < 0.01). The L/Pr increased at the beginning of CI but accelerated to increase during WI in both groups, with significantly prolonged and higher levels in the group with longer CI (P < 0.01).

Conclusions

Extended CI results in increased intrahepatic glycogenolysis, delayed restoration of aerobic glycolysis, and prolonged anaerobic glycolysis shortly after reperfusion. Improvements in glycogen protection and faster restoration of aerobic metabolism during preservation and reperfusion time seem to be necessary in order to improve liver preservation protocols per se.

     

丹红注射液对动脉粥样硬化家兔脂代谢及血管内皮功能的影响

目的观察丹红注射液对动脉粥样硬化(AS)家兔模型脂代谢及血管内皮功能的影响,探讨丹红注射液抗AS作用及可能机制。方法36只新西兰雄性白兔随机均分为正常对照组、高胆固醇组(HC组)、高胆固醇加氟伐他汀组(FC组)和高胆固醇加丹红注射液组(DC组);组织形态学分析AS斑块/内膜面积比值及斑块最厚处内膜厚度/中膜厚度比值;酶法检测血脂;酶法测定血清一氧化氮(NO)和血浆内皮素(ET)水平。结果DC组与HC组比较,血清TC、LDL-C明显降低(P<0.05);血管AS病变显著减轻(P<0.05),血清NO水平显著增高(P<0.05);血浆ET水平显著降低(P<0.05)。结论丹红注射液对实验性AS具有抑制作用,其作用机制可能为①降低血清TC和LDL-C水平,延缓AS斑块的形成;②调节血管内皮细胞生成和释放NO、ET,保护血管内皮细胞功能,进而产生抗AS作用。     

广东省住院患者糖脂代谢状况与合并症调查

目的 了解住院病人糖和脂代谢状态、调脂达标现状及对合并症的影响,以提高临床医务工作者对糖、脂代谢紊乱认知意识和防治水平.方法 采用横断面调查方法,对广东省10家大学附属医院同期住院的8753例患者登记病史、空腹血糖(FBG),行体脂和血脂分析.已诊断糖尿病(PDM)和FBG≥5.6 mmo]/L未诊断精尿病患者填写病例报告表;5.6 mmol/L≤FBG≤6.9 mmol/L者行口服葡萄糖耐量试验(OGTT).结果 PDM和未诊断糖尿病FBG≥5.6 mmol/L资料完整者1067例.未诊断糖尿病5.6 mmol/L≤FBG≤6.9 mmol/L行OGTT检查者占65.8%(325/494).PDM 447例,占41.9%,新诊断糖尿病(NDM)占21.7%,糖调节异常(IGR)占29.1%,正常糖耐量(NGT)7.3%.TG水平NDM组和PDM组高于NGT组和IGR组(P值均<0.05),HDL-C水平NGT组高于糖耐最异常各组(P值均<0.05).血脂异常的比例IGR组52.5%,NGT组56.4%,糖尿病(NDM+PDM)组69.6%.22.8%PDM患者接受系统调脂治疗,达标率3.4%.IGR、NDM和PDM组BMI和腰围大于NGT组(P值均<0.05),腰围PDM组大于IGR组(P<0.01).PDM组合并1种以上血管病变者占72.8%,NDM患者9.7%并发糖尿病肾病,0.2%并发糖尿病视网膜病变.结论 住院糖尿病和IGR患者合并脂质代谢异常的比例显著高于NGT住院患者,以高TG和低HDL-C血症为主.住院PDM患者合并血管病变的比例显著高于其他患者,部分NDM和IGT患者已并发微血管病变.