不同物理因子对新乌头碱体外透皮吸收的影响

目的观察不同物理因子促新乌头碱(MA)透皮吸收的效果,同时测定新乌头碱透入量与作用时间的关系。方法首先采用高效液相色谱法(HPLC)建立新乌头碱的色谱峰(Y)与含量(X,μg)的标准曲线。选取成年大鼠28只,将其随机分成4组(每组7只)。各组大鼠分别在直流电场、低频脉冲电场、超声场及自由透皮条件下进行离体鼠皮的透皮吸收实验。分离各组大鼠背部皮肤,建立离体透皮吸收模型,采用V-C渗透池,将离体鼠皮置于供药池及接受池之间,角质层面向供药池侧。以含生药3 g／ml浓度的生川乌提取液作为释放液,生理盐水作为接受液。各组分别于实验开始后10,20,30,40及50 min时从接受液中取样,根据已求得的Y-X标准曲线测定各取样液中新乌头碱的浓度以及各时间点(t,min)新乌头碱的累积渗透量(Q,μg／cm2)。通过Q-t曲线图求得新乌头碱体外透皮吸收的渗透速率(J,μg·cm-2·min-1),并以此为参数观察不同物理因子干预条件下新乌头碱的透皮吸收状况以及药物透过量与时间的关系。结果在新乌头碱体外透皮吸收实验开始后50 min内,检测到自由透皮组、超声促透组、脉冲电促透组、直流电促透组的渗透速率J分别为4．168, 6．111,12．268及12．623μg·cm-2·min-1;对各组的曲线渗透速率进行统计学分析,发现超声促透组和自由透皮组间差异无统计学意义(P>0．05);脉冲电促透组、直流电促透组分别与自由透皮组比较,发现其间差异均有统计学意义(P<0．05);直流电促透组与脉冲电促透组比较,其间差异无统计学意义(P>0．05)。在整个实验过程中,各组新乌头碱的累积渗透量均呈持续性增长。结论在体外透皮吸收过程中,超声对新乌头碱的促透作用不大,直流电和脉冲电的促透作用较明显;直流电与脉冲电促透作用两者间比较,发现差异无统计学意义(P>0．05);各组自实验开始后50 min内,其新乌头碱的透皮量均随时间延长而持续增多。     

乌头碱直流电离子导入对大鼠关节炎的镇痛作用及其机制探讨

目的探讨乌头碱直流电离子导入对大鼠关节炎的镇痛机制。方法Wistar大鼠45只，随机分为空白对照组（空白组，n=15）、致炎模型组（模型组，n=15）和乌头碱直流电离子导入治疗组（治疗组，n=15），每组又根据观察时间点分为1d、5d和10d亚组，每亚组5只。制作大鼠佐剂性关节炎（AA）模型后，治疗组行乌头碱直流电离子导入治疗，于实验第1，5和10天分别取3组大鼠下丘脑、脑干组织及局部炎性组织，采用放射免疫法测定大鼠下丘脑β-内啡肽（β-EP）含量，采用高效液相色谱法测定大鼠脑干5-羟色胺（5-HT）、去甲肾上腺素（NE）及局部炎性组织5-HT含量。结果模型组大鼠各时间点踝关节炎性组织5-HT的含量明显高于空白组（P〈0．01）；治疗组乌头碱导入治疗1d后，局部炎性组织5-HT含量与模型组比较，差异无统计学意义（P〉0．05），治疗5d及10d后，5．HT含量明显低于模型组（P〈0．05）。模型组大鼠各时间点脑干5-HT含量均明显低于空白组，下丘脑β-EP明显高于空白组（P〈0．05）；治疗组治疗5d及10d后，脑干5-HT和下丘脑β-EP含量均明显高于模型组（P〈0．05）。模型组大鼠各时间点脑干NE含量与空白组比较，差异无统计学意义（P〉0．05）；治疗组治疗5d及10d后，脑干NE含量明显高于模型组（P〈0．05）。结论多次乌头碱直流电离子导入治疗能降低炎性组织局部5-HT含量，升高脑干5-HT、NE和下丘脑β-EP的含量，产生镇痛作用。     

乌头直流电离子导入对佐剂性关节炎大鼠痛阈的影响

目的：观察乌头直流电离子导入对关节炎大鼠痛阈的影响。方法：Wistar大鼠45只，随机分为空白对照组(n=15)，致炎模型组(n=15)和乌头直流电离子导人治疗组(n=15)。制作大鼠佐剂性关节炎，测量乌头直流电离子导人前后佐剂性关节炎大鼠踝周径．以热板反应潜伏期为指标观察大鼠实验后ld，3d，5d，7d，10d的痛阈变化以及乌头直流电离子导入治疗后佐剂性关节炎大鼠治疗局部皮肤的组织学变化。结果：①模型组大鼠各时间点热板反应潜伏期均较空白组明显缩短：治疗组大鼠各时间点反应潜伏期均较模型组延长。②模型组大鼠各时间点踝关节周径均较空白组明显增大：乌头导入治疗1d，3d踝关节周径较模型组无明显变化，5d，7d，10d踝关节周径较模型组减小。结论：乌头直流电离子导入可明显提高佐剂性关节炎大鼠痛阈，治疗局部可见真皮层内毛细血管扩张。     

月桂氮Zhuo酮对氟脲嘧啶直流电导入的促进作用

为研究皮肤促渗剂月桂氮Ｚｈｕｏ酮（Ａｚｏｎｅ）对药物直流电导入的促进作用，我们采用大鼠腹部皮肤作模型，在双室扩散池中研究药物氟脲嘧啶（５－ＦＵ）两种解离状态下的直流电导入，并以被动扩展作对照。其结果，药物以解离型或非离型存在时，皮肤经月桂氮Ｚｈｕｏ酮预处理后直流电导入的伪稳态透皮速率比单用直流电导入的速率分别增大１．４和２．７倍，说明了月桂氮Ｚｈｕｏ酮预处理和直流电导入有协同作用。     

极性交替直流电及其离子导入作用的实验研究

将大鼠和健康自愿者随机分为直流电组和极性交替直流电组,分别用不同电流强度和通电时间,观察大鼠皮肤PH值变化,皮肤损伤情况及~131I人体离子导入量的实验.结果表明:直流电组电流强度0.1mA/cm~2通电60min,PH值已明显变化并出现皮肤烧伤;而极性交替直流电组电流强度0.5 mA/cm~2通电40min以内,pH值无明显变化,电极下皮肤无烧伤.在人体离子导入量观察中,其耐受的电流强度、时间以及离子导人量均具有更大的优越性.并探讨了导人原理.对指导直流电的临床应用有重要意义.     

附片水煎制剂中乌头类生物碱水解物含量测定研究

目的考察用乌头碱水解物、新乌头碱水解物、次乌头碱水解物的含量作为附片水煎剂及附片质量控制指标的可行性。方法采用高效液相色谱分析法,色谱柱为ShimpackCLC-ODS,以甲醇-乙酸铵溶液（O．2mol／L）（200：210）为流动相,流速1．0mL／min,检测波长为241nm,对乌头碱新,乌头碱、次乌头碱水解物的含量测定进行研究。结果3种水解物检测方法测得水解物在进样范围有良好线性关系,重现性好,稳定性好,生药提取时间宜选定为40min,不同批号的附片中3种水解物含量差别较大。结论此方法简便,使用新乌头碱、乌头碱、次乌头碱的水解物作为含附片制剂的质量控制指标是可行的。     

热叠加低频调制中频脉冲电实验研究

实验证明，热叠加调制脉冲电能提高正常人皮肤温度和痛阈，降低皮肤电阻率。通过半透膜实验及人体实验，比较了热叠加否的两组脉冲电，直流电，脉冲直流电透入量，结果表明热叠加组透过亚甲蓝最高，１／４脉冲组最少；人体导入实验结果，＾１３１Ｔ导入率热叠加组最高为７．６２±０．２１，单纯１／２脉冲电组最低为２．２２±０．２２，两组差异有非常显著性，＾１３１Ｉ通电１５分钟以后皮肤扩散率直流电组最高，３４小时热叠加组     

50kHz方法脉冲电流与直流电药物离子导入的比较

目的　研制 5 0kHz方波脉冲电疗仪 ,以建立新型药物离子导入系统。方法　采用5 0kHz方波脉冲电流 ,体外测定双氯灭痛钠药物凝胶透皮速率和累积渗透量 ,测定人体皮肤阻抗 ,观察对疼痛性疾病的镇痛作用和治疗时的电流密度 ,以直流电作对照。结果　 5 0kHz方波脉冲电流与直流电在相同电流密度时 ,药物的透皮速率和累积渗透量差异无显著性 (P >0 .0 5 ) ,但人体局部皮肤阻抗值分别为 ( 6 .70± 3 .74)kΩ ,( 14.2 5± 6 .2 1)kΩ ,(P <0 .0 1) ,电流密度 (P <0 .0 1) ,镇痛作用 (P <0 .0 1) ,均显示差异有非常显著性。结论　 5 0kHz方波脉冲电流可以取代传统的直流电进行离子导入 ,为药物经皮吸收提供了良好的运载电流     

急性草乌中毒兔血浆毒性成分及组织病理学改变的研究

目的 观察急性草乌中毒时的血浆毒性成分及组织病理学改变。方法 选择日本大耳白兔8只，给予草乌酒灌胃染毒制备急性草乌中毒模型。记录心电及血压变化，并于染毒后0．5、1、2、3和6h采血测定血浆乌头碱、新乌头碱、次乌头碱的浓度；取心室肌、肝脏、大脑皮质，光镜下观察组织病理学改变。结果 染毒后动物迅速出现心律失常、血压下降的进行性加重趋势[染毒前：（121.98&#177;16．77）／（110．66&#177;8.78）mmHg，染毒1h：（102.98&#177;8.34）／（90.22&#177;5.85）mmHg，染毒2h：（66．81&#177;9．13）／（53.40&#177;6．32）mmHg，1mmHg-0.133kPa，P均〈0．013；血浆乌头碱、新乌头碱和次乌头碱浓度均持续升高，以染毒1h和2h的差异最为显著[乌头碱；（4．72&#177;3．26）μg／L比（18．48&#177;12．46）μg／L，新乌头碱：（21.52&#177;10．18）μg／L比（345.12&#177;81.36）μg／L，次乌头碱：（2．33&#177;0．70）μg／L比（23.66&#177;19．30）μg／L，P〈0.05或P〈0．013；两者呈正相关。光镜下观察，心肌、肝、脑组织均可见充血、水肿、细胞浸润等病理学改变。结论草乌毒性剧烈，吸收快，心律失常的严重程度与血浆毒性成分浓度呈正相关，早期清除血浆毒性成分是治疗的关键。     

穴位贴敷天灸透皮吸收剂对哮喘豚鼠血清肿瘤坏死因子α的影响

背景：中国古代天灸疗法与现代中药经皮控释给药系统相结合研制而成的透皮吸收剂，经皮肤给药除了能起到局部作用外，还可以透过皮肤进入血液循环，发挥全身作用。目的：观察穴位贴敷天灸透皮吸收剂对哮喘豚鼠血清肿瘤坏死因子α水平的影响。设计、时间及地点：随机对照动物实验，于2007-10／2008-09在南方医科大学中药制剂实验室、广州中医大学动物实验中心完成。材料：48只豚鼠随机分为正常对照组、模型对照组、天灸透皮吸收剂药贴组及地塞米松组，每组12只。方法：用卵蛋白致敏法制作实验性豚鼠过敏性哮喘模型，诱喘成功后，天灸透皮吸收剂药贴组在哮喘发作次日开始进行穴位贴敷治疗。穴位选择：大椎穴、肺俞（双）穴、肾俞（双）穴。每次敷药6h，隔日治疗1次，共治疗7次。地塞米松组采用腹腔注射地塞米松0．5mg／kg，治疗时间与次数同天灸透皮吸收剂药贴组。模型对照组和正常对照组不给予任何干预治疗。主要观察指标：在末次诱喘后2～4h内，采用酶联免疫吸附法测定各组豚鼠血清肿瘤坏死因子α水平。结果：24只豚鼠进入结果分析，每组6只。天灸透皮吸收剂药贴组、地塞米松组豚鼠血清肿瘤坏死因子α水平明显低于模型对照组（P〈0．05）：天灸透皮吸收剂药贴组、地塞米松组豚鼠血清肿瘤坏死因子a水平稍高于正常对照组（P〈0．05）：天灸透皮吸收剂药贴组与地塞米松组豚鼠血清肿瘤坏死因子α平相当，差异无显著性意义（P〉0．05）。结论：穴位贴敷天灸透皮吸收剂药贴与地塞米松均能降低哮喘豚鼠血清肿瘤坏死因子α水平，从而减轻免疫炎性反应及气道高反应性。     

不同的抗原修复方法对肺癌组织TTF-1、P53、SPB等6种抗体表达的影响

目的探讨不同的修复方法配搭不同修复液对肺癌组织TTF-1、P53、SPB等6种抗体表达的阳性数量及强度是否存在影响。方法10例肺癌患者标本,分别用微波、高压配搭柠檬酸缓冲液(pH6.0)、EDTA缓冲液(pH8.0、pH9.0)处理,分别滴加一抗,采用二步法,进行免疫组化标记,分别由两位病理医师进行独立看片,并进行结果比较。结果不同的修复方法配搭不同修复液对这些抗体表达的阳性数量及强度存在影响。结论微波配搭柠檬酸缓冲液对易表达抗体的染色效果佳;高压配搭EDTA缓冲液(pH8.0)对显示细胞核染色效果佳,高压配搭檬酸缓冲液(pH6.0)对显示难表达抗体的细胞浆、细胞膜染色效果佳。     

Evaluation of a system for intragastric pH monitoring of intensive care unit patients: preliminary report.

BACKGROUND: A new pH probe-tipped nasogastric sump tube is available to monitor gastric pH conveniently. This study assesses its ability to measure gastric acidity accurately. METHODS: The accuracy of the combined pH probe nasogastric tube (GrapHprobe ST) was determined by comparing it with standard buffer solutions (pH 1.0, 2.0, 4.0 and 7.0) traceable to the National Institute of Standards and Technology. Gastric pH values obtained were compared with values obtained using indicator paper and a calibrated glass electrode on gastric aspirate. RESULTS: Although statistically significant differences were found in vitro between the pH of three of the buffer solutions and the pH values obtained by the nasogastric sump tube, the results were within 0.5 pH unit. When rounded to the nearest pH unit, all values were the same as the buffer solutions. No significant difference was found in the pH values obtained during in vivo testing. CONCLUSIONS: The GrapHprobe ST measured gastric pH within reasonable accuracy in this small series.     

Apoprotein B in fasting and postprandial human jejunal mucosa.

We tested whether apoprotein B is present in fasting and postprandial human duodenojejunal mucosa because lipoprotein-like particles are visualized by electron microscopy within the smooth endoplasmic reticulum and the Golgi cisternae of these absorptive cells. Duodenojejunal biopsies from normal volunteers were incubated in citrate buffer and were shaken in 1% EDTA so that absorptive cells could be freed from underlying tissue. Apoprotein B was determined by double-antibody radioimmunoassay in homogenates of absorptive cells. The preparations of absorptive cells were shown to be uncontaminated by plasma lipoproteins; they did not contain any albumin by immunodiffusion able to detect 2 mug/ml. They adsorbed less than 0.1% of 125I-low density lipoprotein which was added to the citrate buffer. Cell preparations from suction biopsies of human rectum contained no detectable apoprotein B. Duodenojejunal absorptive cells from 22 fasting subjects contained 3.2 +/- 0.5 mug of apoprotein B per 100 mg (wet wt) of biopsies or 1.3 mug of apoprotein B per mg of total cell protein. The amount of apoprotein B per milligram of cell protein fell to 0.3 mug in 14 of these individuals whose mucosa was also sampled 45 min after instilling fat intraduodenally. These experiments provide immunochemical evidence that human duodenojejunal absorptive cells contain apoprotein B. This technique should be valuable for studying the physiology of intestinal lipoproteins in absorption and in patients with hyperlipidemia.     

Relationship between in vitro activities of amphotericin B and flucytosine and pH for clinical yeast and mold isolates

In this study, we investigated the pH dependency of the in vitro activities of amphotericin B (AMB) and flucytosine (5FC) against Candida spp., Cryptococcus neoformans, Aspergillus fumigatus, Rhizopus spp., and Scedosporium prolificans in RPMI 1640 buffered with citrate buffer (pH 4.0, 5.0, 5.4, and 6.0), citrate-phosphate buffer (pH 5.4, 6.0, 6.4, and 7.0), and 3-[N-morpholino]propanesulfonic acid (MOPS) (pH 6.4, 7.0, 7.4, and 7.9). For 5FC, no significant differences were found between MICs obtained with the different buffers, while for AMB, significant differences were found. The MICs obtained with citrate-phosphate buffer were approximately 1 twofold-dilution step higher than the MICs obtained with MOPS. We demonstrated that the in vitro activities of AMB and 5FC against yeast and mold isolates were pH dependent. The in vitro activity of AMB decreased when the pH was lowered, while the in vitro activity of 5FC increased. The effect of the pH on the in vitro activities was dependent not only on the antifungal agent tested but also on the microorganism. For AMB, there was a nonlinear relationship (median r(2), 0.864) for Candida spp., C. neoformans, A. fumigatus, and Rhizopus spp. over the pH range tested. The mean MICs ranged from 0.5 to 2.52 microg/ml at pH 7.0 and from 20.16 to 32 microg/ml at pH 5.0. For S. prolificans, there was no relationship. For 5FC, there was a linear relationship for Candida spp. (median r(2), 0.767) and a nonlinear relationship for C. neoformans and A. fumigatus (median r(2), 0.882) over the pH range tested. The mean MIC values ranged from 0.125 to 1,024 microg/ml at pH 7.0 and from 0.02 to 4 microg/ml at pH 5.0. For Rhizopus spp. and S. prolificans, the relationship could not be determined, since the MIC was >1,024 microg/ml over a pH range of 4.0 to 7.9.     

氯羟二苯醚杀灭微生物效果及其影响因素研究

氯羟二苯醚为白色结晶粉末,以丙二醇溶解,再用水稀释制成消毒液。采用载体定量试验法,观察其杀灭微生物效果及影响因素。结果,用含氯羟二苯醚 ２００ ｍｇ／ Ｌ的溶液（ ｐＨ ６．５）对不锈钢片表面金黄色葡萄球菌、大肠杆菌作用 ３ ｍｉｎ,对白色念珠菌作用 １０ ｍｉｎ,杀灭率均≥９９．９５％。杀菌效果因有机物增多、作用温度降低而下降,在消毒液 ｐＨ值为 ３．０～７．０时无明显变化, ｐＨ值为 ９．０时明显下降。该剂于 ５４℃下存放 １４ ｄ,杀菌作用无明显变化。     

Transdermal Kinetics of A Mercurous Chloride Beauty Cream: An In Vitro Human Skin Analysis

Background: Crema de Belleza-Manning is a popular mercurous chloride-containing beauty cream used to smooth and lighten the complexion and treat acne. Hundreds of people in the Southwestern US border states have been identified with elevated (>20 μg/L) urine mercury levels believed to be secondary to using this cream. The kinetic characteristics of percutaneous mercury absorption are incompletely defined. The objective of this study was to determine the transdermal kinetics of two formulations of mercurous chloride from a beauty cream in an in vitro human skin model. Methods: A proprietary formulation and an aqueous formulation of the beauty cream were studied using modified Franz diffusion cells. Mercury content in the skin samples and the underlying diffusion buffer was determined using atomic absorption spectrophotometry. Results: A rapid initial increase in mercury content both in the skin and the buffer was noted for both formulations. Mercury concentrations in the aqueous samples were significantly (p < 0.05) higher in both the skin and the diffusion buffer compared to parallel samples containing glycerol. Conclusions: Mercury was readily absorbed through the skin in this in vitro human skin model. The aqueous preparation had a markedly increased rate and extent of mercury absorption relative to the proprietary formulation.     

Optimisation of an enzymatic method for beta-galactosidase

BACKGROUND: The enzyme beta-galactosidase present in the Kupffer cells of the liver has potential as a marker of liver dysfunction prior to transplantation. Spectrophotometric methods have insufficient sensitivity. METHODS: Fluorimetric methods have the required sensitivity and we have optimised such a method in a microtitre plate format to improve its utility. beta-galactosidase acts on the substrate 4-methylumbelliferyl-galactoside (MUG) to produce 4-methylumbelliferone (4-MU), detected fluorimetrically with excitation wavelength 355 nm and emission wavelength 460 nm. RESULTS: Reaction conditions in a citrate-phosphate buffer were optimised to give maximal enzyme activity: pH was optimal at 4.4 (range investigated 3.6-5.0) and substrate concentration at 3.33 mmol/l. A small specimen volume (10 microl) in 80 microl of substrate solution produced adequate fluorescent yield after an incubation period of 30 to 60 min at 37 degrees C. Reaction was terminated by addition of 200 microl of glycine-NaOH, pH 12.8. The assay is linear to 3,000 U/ml. The intra-assay coefficient of variation (CV%) at 50, 502, and 2,012 U/ml was 4.7, 3.1, and 3.4, respectively (n=10). Inter-assay CV% at 51, 496, and 1,986 U/ml was 7.0, 4.0, and 3.9, respectively (n=10). CONCLUSIONS: The assay has greater practical utility and demonstrated significant differences in the perfusate beta-galactosidase between cold-stored and warm-perfused livers in a porcine model of transplantation.     

Separation of CK isoenzymes on DEAE-sephadex A-50 at neutral pH

The MM, MB and BB isoenzymes of human creatine kinase (CK) were separated by elution from micro-columns of DEAE-Sephadex A-50 with Tris buffer containing increasing concentrations of NaCl at pH 7.0, instead of pH 8.0 as has commonly been used. Since pH 7.0 is close to the pH optimum of CK, this allowed the use of four times larger aliquots of the eluates for the estimation of CK activity and, consequently, a 4-fold increase in sensitivity. Using serum specimens from patients with acute myocardial infarction, there was a good correlation of the CK-MM (r = 0.99) and CK-MB (r = 0.93) activities obtained with the two buffer systems. Similarly, normal sera had CK-MB and CK-BB activities of less than 2 U/l with both buffer systems. Comparison of the composition of serum proteins in the eluates by conventional electrophoresis revealed that although the distribution of CK isoenzymes separated by the two buffer systems was similar, the distribution of proteins at pH 7.0 showed an appreciable shift of protein from the MB to the MM eluates.     

To determine the effects of ultraviolet light, natural light and ionizing radiation on pyridinium cross-links in bone and urine using high-performance liquid chromatography

The aims of the study were to characterize the denaturation of urinary free and conjugated pyridinoline (Pyr) and deoxypyridinoline (Dpyr) on exposure to ultraviolet (UV) and natural light at different pH levels and to study the effects of X- and γ-irradiation on Pyr and Dpyr in urine and in the mineralized and non-mineralized compartments of human bone. Urine samples from six normal subjects, adjusted to pH 3.0, 7.0 and 9.0, were exposed to UV light for up to 3 days. Urine collections (2 mL and 24 h) from three subjects, pH adjusted to 1.0, 2.0, 3.0, 4.0 and 5.0, were exposed to natural light for up to 1 day. Urine samples and bone slices from seven human cadaveric femurs were irradiated with increasing doses of X-rays (0–100 Gy) and high-dose γ-radiation (28kGy). Mineralized and non-mineralized bone were separated using a modification of a published method employing heat denaturation followed by trypsin hydrolysis and analysed for Pyr, Dpyr and hydroxyproline (Hypro). The rate of UV photolysis of urinary Pyr and Dpyr increased with pH and was faster in the free fraction (after 3 days’ exposure: free Pyr and Dpyr at pH 7.0 vs. 9.0, P<0.05, conjugated pH 3.0 vs. 9.0, P<0.05). Exposure to natural light for 3 h did not significantly decrease urinary Pyr and Dpyr in either sample collections, but levels were reduced in the 2-mL aliquots after exposure for 1 day (P<0.05). X-irradiation of urine and bone did not affect Pyr and Dpyr. Pyr content was similar in both bone compartments (Pyr/Hypro=0.12±0.004), but Dpyr was higher in the non-mineralized compartment (Dpyr/Hypro=0.047±0.002 vs. 0.038±0.002, P<0.001). UV light and γ-irradiation result in denaturation of pyridinium cross-links in urine. These cross-links are present in both the mineralized and non-mineralized bone compartments but are not affected by the doses of γ-irradiation that denature these cross-links in urine.     

毛细管区带电泳用于血浆蛋白质混合物的分析

目的探索建立采用毛细管区带电泳(CZE)法分析血浆相关蛋白质的条件和方法。方法 1)以血浆COHN法组分Ⅳ(CFⅣ)抽提液为样品,代表蛋白质混合物,分别从波长、基本缓冲液、添加剂、PH、温度等方面,摸索CEZ分析蛋白质混合物的较优条件和方法;2)将摸索得到的较优条件下的CZE分析CFⅣ抽提液的结果,并与通过高效液相色谱(HPLC)法、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法及双向电泳(2-DE)法分析CFⅣ抽提液的结果比较;3)采用较优条件下的CZE法分析标准血浆、标准血浆经离子交换层析(IEC)的FⅧ粗提液等,考察该条件是否适用于血浆中的其他组分。结果 1)CZE法分析CFⅣ抽提液的较优条件为:20℃以214 nm紫外吸收波长,0.5 psi压力进样10 s,10 kV恒压分析50 min,缓冲体系为pH 9.0的0.04 mol/L硼酸硼砂、0.05%SDS、0.007 5%腐胺;2)采用此条件的CZE法分析CFⅣ得到14个蛋白峰、分析时间50 min,HPLC法分析得到10个蛋白峰、分析时间60 min,SDS-PAGE法分析得到11条蛋白条带、分析时间120 min,2-DE法分析得到14个蛋白点数(与CZE分析的蛋白峰数数量相同)、分析时间3 d;3)CZE法用于标准血浆、标准血浆经离子交换层析的FⅧ粗提液的分析可分别分辩出22和11个蛋白峰。结论以CZE法分析血浆相关蛋白混合物与现有分析方法比较,能达到较好的分析效果,经摸索确定的较优分析条件具有一定的通用性。