共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
4.
5.
Popesco MC Lin S Wang Z Ma ZJ Friedman L Frostholm A Rotter A 《Neurobiology of aging》2008,29(5):774-788
Changes in specific cerebellar molecules contribute to impaired balance and motor coordination frequently observed in aged individuals. Serial analysis of gene expression (SAGE) was used to construct six libraries from adult and aged mouse cerebella. Combined unique tags for each group revealed 325 genes that were differentially expressed (p-chance=0.05). Four additional tests (mixed effect model, t-test, Wilcoxon rank-sum and z-test) were used to reduce the likelihood of false positives. The first two tests accounted for intra-group variation, the third for outliers and the fourth was a probability test similar to p-chance. Twenty-nine genes were identified by at least two of the four tests as being differentially expressed in the aged cerebellum. Three of these genes are, as yet, unidentified and likely to represent novel genes involved in the cerebellar aging process. The largest group of genes revealed by the combined tests was related to glycerophospholipid metabolism, and included phosphoethanolamine, phosphatidic acid and diacylglycerol synthetic enzymes, suggesting that alterations in lipid biosynthesis may, at least in part, underlie changes in conductivity in the aged cerebellum. 相似文献
6.
7.
Discovery of novel tumor markers of pancreatic cancer using global gene expression technology 总被引:20,自引:0,他引:20
Iacobuzio-Donahue CA Maitra A Shen-Ong GL van Heek T Ashfaq R Meyer R Walter K Berg K Hollingsworth MA Cameron JL Yeo CJ Kern SE Goggins M Hruban RH 《The American journal of pathology》2002,160(4):1239-1249
8.
9.
10.
11.
12.
13.
The limb phenotypes of Tbx2 and Tbx3 mutants are distinct: loss of Tbx2 results in isolated duplication of digit 4 in the hindlimb while loss of Tbx3 results in anterior polydactyly and posterior oligodactly in the forelimb. In the face of such disparate phenotypes, we sought to determine whether Tbx2 and Tbx3 have functional redundancy during development of the mouse limb. We found that sequential loss of alleles generates defects that are not simply additive of those observed in single mutants and that multiple structures in both the forelimb and hindlimb display compound sensitivity to decreased gene dosage. 相似文献
14.
15.
Treatment of pregnant mice at the egg cylinder stage with retinoic acid (RA) has caused ectopic hindlimbs in the offspring. Proposed causes of ectopic hindlimbs include homeotic transformation or multiple axis formation. Two mouse strains were determined to be divergent in susceptibility to this malformation (C57BL/6N, highly sensitive; SWV/Fnn, less sensitive). Ectopic limbs were hindlimbs (expressing Pitx1 and Tbx4 but not Tbx5), yet they also expressed the predominantly forelimb Hoxb8. Ectopic body axis formation was indicated by gene expression for ectopic primitive streaks, notochords, and nodes, as well as inhibition of anterior visceral endoderm and mesodermal migration. The earlier in development that embryos were examined, the higher the rate of ectopic hindlimb development and axis formation. Ectopic axis formation and cell migration inhibition had the same strain susceptibility as the dysmorphogenesis. We propose that all extra hindlimbs were derived from ectopic axis formation, perturbation of which is genetic background dependent. 相似文献
16.
基因表达系列分析及其应用前景 总被引:6,自引:0,他引:6
基因表达系列分析(Serial analysis of gene expression,SAGE)是一种研究真核细胞表达基因信息的高通量检测技术,它能对细胞内所有表达基因进行定性与定量分析。近年来此技术广泛应用于获得表达基因谱的研究,并且可以发现新基因信息,还可发现基因的靶向定位以及对其它基因的影响,明确表达基因的功能。本文就SAGE的原理、实验方案、技术发展与演变及其应用前景进行详细介绍。 相似文献
17.
18.
19.
20.
We recently reported the positional cloning of a homeobox gene involved in
the pathogenesis of Rieger syndrome, RIEG1 , and its mouse homolog, Rieg1 .
Rieg1 (also independently described as Pitx2) is highly homologous to the
Ptx1/Potx gene product, suggesting that there may be additional members of
this novel Pitx family. The Pitx genes play an important role in eye,
tooth, pituitary and umbilical region development as evidenced by Rieger
syndrome and iris hypoplasia phenotypes, resulting from mutations in the
RIEG1 gene and by expression studies. In order to characterize further the
Pitx gene family we searched mouse cDNA libraries to identify additional
members. A new gene was isolated which encodes a homeoprotein with strong
homology to the other Pitx proteins and 97-100% identity in the homeodomain
itself, suggesting that this is a third member of the family, Pitx3 . In
whole mount in situ hybridization on mouse embryos ranging from 8.5 to 11.5
days post-coitum (d.p.c.), Pitx3 mRNA was seen only in the developing lens
starting at day 11. Hybridization on cross- sections revealed strong
signals in the lens vesicle in 11 d.p.c. embryos and throughout the lens,
particularly in the anterior epithelium and equator region in 15 d.p.c.
embryos. Pitx3 was mapped close to aphakia on mouse chromosome 19. The
aphakia homozygous mouse is characterized by small eyes lacking a lens,
which fail to develop beyond 11 d.p.c. These data make Pitx3 a strong
candidate gene for the aphakia phenotype in the mouse and suggest a role
for the human homolog in congenital lens malformations.
相似文献