共查询到20条相似文献,搜索用时 62 毫秒
1.
不同海拔高度健康人超氧化物歧化酶和丙二醛的测定 总被引:1,自引:1,他引:1
对不同海拔高反的西安市(海拔340m)、西宁市(2260m)、茶卡镇(3100m)和甘德地区(4080m)共1089例健康人进行了红细胞——超氧化物歧化酶(SOD)和血清丙二醛(MDA)测定。结果表明:随着海拔高度的升高和大气压、大气氧分压的降低,血清MDA明显增加(P<0.01),红细胞SOD显著降低(P<0.01)。相关分析表明:MDA与大气氧分压呈明显负相关(r=-0.979,P<0.05),SOD与大气氧分压呈明显正相关(r=0.984,P<0.05)。本文对长期生活在高原低氧环境下居民MDA升高和SOD降低的原因进行了探讨。 相似文献
2.
ApoE基因敲除鼠12周游泳运动前后纤溶激活功能的改变 总被引:3,自引:0,他引:3
目的:探讨ApoE在长期有氧运动中影响纤溶激活作用中的有关机制。方法:以ApoE基因敲除(ApoE-/-)鼠为实验组、以相同遗传背景的C57BL/6J鼠为对照组建立运动模型。结果:12周游泳后,对照组鼠TG(0.96±0.30vs0.54±0.070mmol/L,P<0.01)和PAI(0.92±0.07vs0.80±0.09AU/ml,P<0.05)降低,tPA升高(0.60±0.12vs1.04±0.23IU/ml,P<0.01);而ApoE-/-鼠的TG(1.28±0.34vs1.29±0.32mmol/L,P>0.05)和PAI(0.74±0.10vs0.79±0.04AU/ml,P>0.05)均没有显著变化,tPA(0.71±0.15vs0.97±0.17IU/ml,P<0.01)升高。结论:长期有氧运动使对照小鼠纤溶激活作用明显改善,其机制与TG和含ApoE脂蛋白水平降低导致LRP清除tPA-PAI作用加强、PAI抑制作用减弱及tPA合成水平增加有关。Ap oE-/-鼠运动后,PAI水平稳定而tPA活性增加,提示tPA合成作用增强。 相似文献
3.
缺氧对肺动脉内皮细胞和平滑肌细胞分泌血管活性肽的影响 总被引:3,自引:0,他引:3
用放射免疫测定和免疫组化法缺氧对培养的小牛肺动脉内皮细胞(PAEC)和肺动脉平滑肌细胞(PASM)自分泌心钠素(ANP)、血管紧张素Ⅱ(ATⅡ)和内源性洋地黄因子(EDLF)的影响。无氧培养24h末,PAEC分泌ANP减少43.5%(P〈0.001),ATⅡ和EDLF呈明显负相关(r为-0.88和-0.786,P〈0.01),细胞内ANP阳性颗粒也显著减少(P〈0.001);PASM分泌ANP无显 相似文献
4.
目的观察急性低压缺氧后内皮素前体原(ppET)和内皮素-1(ET-1)的变化规律,探讨ET-1升高的机理。方法将实验大鼠分成三组,观察急性中度低压缺氧30min时及6h后ppET和ET-1的含量变化,分析其相关性。结果①急性中度低压缺氧30min后ppET和ET-1均明显升高(P<0.05或0.01),脱离缺氧后6h,ppET和ET-1有轻度下降,但仍未恢复到对照组水平(P<0.05)。②缺氧前对照组ppET与ET-1呈明显正相关(r=0.93,P<0.001),缺氧30min后,ET-1和ppET虽明显升高,但二者仍呈明显正相关(r=0.90,P<0.001),脱离缺氧后6h,二者有轻度下降,但二者亦呈明显正相关(r=0.80,P<0.01)。结论急性低压缺氧可启动ET基因系统,ET-1的升高是ppET合成和释放增加的结果。急性中度低压缺氧对ET基因启动的时间较长,至少可维持6h以上。 相似文献
5.
为研究高血压病(EH)患者左室重量与外周血淋巴细胞β受体密度的关系,采用超声心动图与3H-双氢阿普洛尔(3H-DHA)放射性配基结合分析法,检测70例EH男性患者左室重量指数(LVMI)与外周血淋巴细胞β受体最大结合容量(βmax)。结果显示:①LVMI>125g/m2患者βmax为(603±101)fmol/107细胞,分别高于LVMI<125g/m2患者[(411±103)fmol/107细胞,P<0.01]和正常人[(367±110)fmol/107细胞,P<0.001];②随着LVMI的增加,外周血淋巴细胞βmax呈现进行性上行调节(二者相关系数r=0.44,P<0.01)。表明高血压病患者左室重量的增加与外周血淋巴细胞β受体密度的上行性调节具有相关关系。可能与高血压病理状态下机体内源性儿茶酚胺系统的激活,使β受体对其细胞生物活化效应的中介作用增强有关。 相似文献
6.
高原人睾丸酮、雌二醇水平与年龄和血红蛋白之间相关性研究 总被引:4,自引:0,他引:4
本资料研究高原地区(3200m)375例正常人(15~85岁)的血浆睾酮(T)、雌二醇(E_2)浓度与年龄和血红蛋白(Hb)等之间的相互关系,结果:①男性T值在60岁前高于平原值(P<0.05),在60岁后,T随龄增加而下降,E_2随龄增而上升,Hb和睾丸体积(V)在50岁后,逐渐下降或减少,Hb与T之间呈正相关(r=0.145,P<0.05);②女性中T随龄增有下降趋势,E_2随龄增而明显下降(r=0.202,P<0.05);T/E_2比值在男性中随龄增而下降,在女性中则上升。提示:在高原低氧环境中,人体为了适应缺氧环境,性激素等发生了相应性变化。 相似文献
7.
高原低氧与循环内皮细胞计数及内皮素-1和心钠素含量的相关性研究 总被引:2,自引:0,他引:2
目的:探讨高原低氧环境下血管内皮细胞(VEC)的损伤情况及功能变化;方法:对从平原(海拔1 400m )进驻海拔3 700m (10 人)与5 380m (20 人)高原第7 天和半年的青年进行循环内皮细胞计数(CEC)、血浆内皮素-1(ET-1)和心钠素(ANP)含量测定,并与平原作对照。结果:在高原缺氧条件下,血浆CEC计数和ET-1、ANP含量明显高于平原(P< 0.01),且随海拔高度的升高而增高(P< 0.01),初入高原时明显高于在高原居住半年的数值(P< 0.05 或P< 0.01)。在不同海拔高度居住不同时间的ET-1与CEC和AVP呈高度正相关;结论:血管活性肽ET-1、ANP分泌可能对急、慢缺氧环境下的心血管系统功能及神经内分泌调节具有重要意义。 相似文献
8.
本文应用火箭免疫电泳法分别测定了不同海拔地区总共573例健康人血清α_1-AT水平,其中上海(海拔5m)359人,西宁(海拔2260m)129人,青海热水(海拔3700m)85人。结果为:上海地区健康人血清α_1—AT水平2.81±0.68g/L,西宁3.91±1.11g/L,热水3.36±0.82g/L。结果表明,健康人血清α_1-AT水平由高到低依次是西宁、热水、上海。经统计学分析三组之间差别有高度显著性意义(P<0.01),而与性别无关(P>0.05)、提示健康人随居住地海拔增高血清α_1-AT水平有增高趋势。这种改变可能与海拔增高,空气中氧分压下降刺激肝细胞和肺泡巨噬细胞合成α_1-AT增加有关,但随着海拔进一步增高,这种作用反而受到抑制。 相似文献
9.
10.
本文测定了未孕妇女、正常孕妇和妊高征孕妇的超氧化物歧化酶(SOD),丙二醛(MDA)和维生素E(VE)水平。结果为正常孕妇SOD低于未孕妇女,而MDA和VE高子未孕妇女(P均<0.01),提示孕妇抗氧化能力可能增强。妊高征孕妇与正常孕妇相比,SOD、VE更低(P<0.01),而MDA则更高(P<0.05),而且妊高征孕妇的MDA与SOD、MDA与VE呈负相关(r=-0.956,-0.7048、P<0.05),提示可能有脂质过氧化反应的增强和氧化能力下降,为妊高征的急性动脉硬化提供了条件。 相似文献
11.
Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version 总被引:5,自引:0,他引:5
E. M. Roos H. P. Roos C. Ekdahl L. S. Lohmander 《Scandinavian journal of medicine & science in sports》1998,8(6):439-448
The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living. 相似文献
12.
13.
14.
Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas (CCF) and their complications such as pseudoaneurysms. Methods: Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% (4/22). A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% (8/22). Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents. 相似文献
15.
Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered. 相似文献
16.
Introduction Ankle sprains are the most common musculoskeletal injury that occurs inathletes particularly in sports that require jumping landing on one foot such assoccer and basketball 《中国运动医学杂志》2008,27(3):378-381
Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8). 相似文献
17.
18.
Martin J.Gibala 《中国运动医学杂志》2008,27(3)
KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief,intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.,≥90%of VO2 peak). 相似文献
19.
20.
Tucker VC Hopwood AJ Sprecher CJ McLaren RS Rabbach DR Ensenberger MG Thompson JM Storts DR 《Forensic science international. Genetics》2011,5(5):436-448
In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex® ESI 16 (European Standard Investigator 16) and the PowerPlex® ESI 17 Systems. The PowerPlex® ESI 16 System combines the 11 loci compatible with the UK National DNA Database®, contained within the AmpFlSTR® SGM Plus® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR® SGM Plus® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex® ESI 17 System amplifies the same loci as the PowerPlex® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR® SGM Plus® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors. 相似文献