骨关节炎急性时相反应蛋白的变化

目的：了解急性时相反应蛋白与骨关节炎的关系，方法：应用Ａｒｒａｙ特殊蛋白全自动分析仪检查２０例骨关节炎患者及２０名正常人血清Ｃ反应蛋白（ＣＲＰ），α１酸性糖蛋白（ＡＡＧ），触殊蛋白（ＨＰＴ）和转铁蛋白（ＴＲＰ）含量，结果：ＯＡ患者血清ＣＲＰ，ＡＡＧ和ＨＰＴ含量升高，ＴＲＦ含量下降，ＣＲＰ与ＡＡＧ，ＨＰＴ及动态血沉存在正相关关系，与ＴＲＦ存在负相关关系，结论：急性时相反应蛋白中关节炎症程度有关，相互     

肾综合征出血热患者血清促肾生长活性的测定

动物或人肾脏“单切”后血中促肾生长因子活性增强,进一步研究发现该物质产生于肾系膜细胞。从尿中分离到这种因子,推测其可能由肾细胞分泌,经尿液排出［１,２］。我们采用原代大鼠肾皮质细胞体外培养和３ＨＴｄＲ掺入法对１０例肾综合征出血热（ＨＦＲＳ）所致急性...     

肾综合征性出血热

老年肾综合征出血热的临床分析；肾综合征出血热患者血清α-肿瘤坏死因子的检测及其意义。     

肾综合征出血热血清酶测定的临床意义

肾综合征出血热血清酶测定的临床意义于德航吴力克逄金聚刘宏王启娟作者单位：２６６０７１青岛，解放军第４０１医院为了探讨肾综合征出血热（ＨＦＲＳ）患者的血清酶变化规律，我们将１９９４年１１月以来收治的５１例ＨＦＲＳ患者，进行七种主要血清酶检测并追踪其动态...     

肾综合征出血热患者血清Zn,Cu测定的临床意义

HFRS患者血清微量元素Zn、Cu的测定国内报告较少。本文就近年来收集的HFRS患者血液标本117份进行了血清Zn、Cu的测定，同时测定了肾功能指标尿素氮（BllN）及肾衰指数（FRI）作为分析时比较，现报告如下。1材料与方法1.1标本来源117价标本取自湖南医科大学附二院传染科近几年来的住院患者。均按1986年南京会议标准，并经检测HFRS特异性抗体阳性而确诊。其中男84例，女33例，年龄35.7士9.2（7～57）岁。临床分期：发热期6例，低血压休克期13例，少尿期38例，移行期16例，多尿期33例，恢复期正工例。临床分型：轻型13例，中型51例，…     

肾综合征出血热患者尿液中病毒结构蛋白的检测及意义

为了阐明肾综合片出血热（ＨＦＲＳ）患者尿液中病毒膜蛋白（ＭＰ）。核蛋白（ＮＰ）的消长与病情及预后的关系，探讨ＨＦＲＳ的发病机制，应用免疫酶斑点法（ＩＥＤＡ）对２６例ＨＦＲＳ患者不同病日的１１８份尿液中病毒结构蛋白抗原进行了检测，结果ＨＦＲＳ患者尿液中ＭＰ和ＮＰ抗原在发病初期阳性率最高，以后逐渐下降，且４～７病日尿液中ＭＰ阳性率高于ＮＰ，７病日后两者无明显差别，同时重度患者ＭＰ水平明显高于中度患者，     

肾综合征出血热血清酶值变化的意义

为了探讨肾综合征出血热（ＨＦＲＳ）患者血清丙氨酸转氨酶（ＡＬＴ）、天门冬氨酸转氨酶（ＡＳＴ）、乳酸脱氢酶（ＬＤＨ）酶值变化的临床意义,我们对１９９６年度１３０例ＨＦＲＳ住院病例作血清酶值测定,同时观察ＡＬＴ、ＡＳＴ、ＬＤＨ与ＨＦＲＳ各病型间及血清肌配（ＳＣｒ）、血小板数（ＰＬｔ）、血异型淋巴细胞（异淋）、尿蛋白与预后的关系。现报道如下。对象与方法１３０例ＨＦＲＳ住院患者,男性９６例,女性３４例,年龄２１～６７岁,平均３９±１０．５岁。按１９８７年全国流行性出血热会议制定的标准诊断与分型,并经金标…     

肾综合征出血热患者血清一氧化氮的检测及其临床意义

肾综合征出血热患者血清一氧化氮的检测及其临床意义崔速南汪明明张娟吴世英于淑丽一氧化氮（ＮＯ）是近年在人体内发现的一种生物活性物质，并已认识到其在多种感染性疾病中发挥着重要作用［１］。为探讨人体内ＮＯ在肾综合征出血热（ＨＦＲＳ）中的作用及其临床意义，我...     

PCR-ELISA用于HFRS病人早期诊断的研究

目的　建立检测HFRS患者血清标本中HV基因的PCR -ELISA方法。方法　设计并合成互补于HVS基因片段的标记引物 ,对 98例HFRS患者血清进行RT -nestedPCR扩增 ,并用酶免方法分析扩增产物。结果　 98例患者不同病日采集的血清标本的RT -nestedPCR平均检出率仅为 6 0 .2 % ,而PCR -ELISA平均检出率为 80 .6 %。结论　PCR -ELISA用于早期HFRS患者的HV基因检测是一种理想的检测方法     

血清急性时相蛋白水平与胃癌临床病理的关系

采用Array特定蛋白分桥系统、全自动速率散射比浊法检测120例胃癌患者手术前的血清急性时相蛋白水平，并与正常对照组比较。结果显示胃癌患者血清C—反应蛋白(CRP)、α1—抗胰蛋白酶(α1-AT)和α—酸性糖蛋白(α—AG)水平显著高于对照组(P＜0．001)，其水平与细胞的分化程度差异无显著性(P＞0．05)；癌细胞浸润胃壁浆膜及肌层组显著高于浸润粘膜及粘膜下层组(P＜0．01)；有淋巴结转移组明显高于无淋巴结转移组(P＜0．01)；Ⅲ、Ⅳ期组显著高于Ⅰ、Ⅱ期组(P＜0．01或P＜0．05)。提示CRP、α1—AT和α—AG在胃癌发展过程中起重要作用，且与胃癌某些临床病理指标密切相关。     

A new acute-phase protein. II. Quantitative determination in the serum of patients with rheumatoid arthritis

An antigen (SF1 or TSGA) originally found in the inflammable synovial fluid of man, which comes from the cytoplasm of neutrophils, possesses the properties of an acute-phase-protein. The production of a specific antiserum against this antigen and the method of its quantitative determination by means of the Mancini-technique are described. In 89 sera of patients with rheumatoid arthritis the antigen was determined. First results of this investigation which speak for a dependence of the antigen concentration in the serum on activity and progression of the disease are reported.     

Profiles of the acute-phase reactants C-reactive protein and ferritin related to the disease course of patients with systemic lupus erythematosus

OBJECTIVE: To obtain insight in the acute-phase response in SLE. METHODS: The clinical history, SLEDAI, CRP and ferritin concentrations were analysed throughout the disease course of 10 SLE patients. RESULTS: During a mean follow-up of 4.8 years, 10 exacerbations (SLEDAI > or = 11) occurred. Throughout the disease course, CRP and SLEDAI correlated positively in 5 patients, whereas the correlation between SLEDAI and ferritin was positive in 7 patients. However, elevated CRP concentrations together with elevated ferritin levels were only observed during 4 exacerbations. Ferritin concentrations were exceptionately high (> 1500 microg/L) during 4 flare-ups. CRP and ferritin levels remained normal during 5 exacerbations. CONCLUSION: SLE is characterised by highly variable and unusual CRP and ferritin responses that do not always reflect the extent of inflammation in individual patients. Despite severe disease activity, ferritin levels can remain well within the normal range, limiting its clinical usefulness as a marker for disease activity.     

Cytokines and acute-phase response markers derangements in patients with obstructive jaundice

BACKGROUND/AIMS: Prolonged acute-phase response and increase of cytokines have been associated with higher mortality and surgical complications. This study investigated the status of cytokines and acute-phase response markers in patients with obstructive jaundice. METHODOLOGY: Forty-one patients were investigated. Endotoxin, tumor necrosis factor-alpha, interleukin-6, nitric oxide, C-reactive protein, liver enzymes, albumin and percentage of weight loss were determined at admission. RESULTS: Endotoxin, interleukin-6 and C-reactive protein were significantly elevated in both benign and malignant obstructive jaundice. Increased plasma levels of tumor necrosis factor-alpha were only detected in malignant tumors (68 vs. 24 pg/mL; P < 0.001). Patients with positive acute-phase response (C-reactive protein > mean + 2 SD of controls) had greater weight loss (P = 0.02), endotoxin (P = 0.03) and interleukin-6 plasma levels (P = 0.05) than those with no inflammatory response. Prolonged biliary obstruction (> 10 days) was associated with higher weight loss (P = 0.04), tumor necrosis factor-alpha (P = 0.003) and interleukin-6 (P = 0.05) plasma levels. CONCLUSIONS: A prolonged high-grade biliary tract obstruction prompted an increase in endotoxin levels, associated with a positive acute-phase response and cytokine elevation.     

A study of acute-phase reactant proteins in Indonesian patients with pulmonary tuberculosis

The levels of 8 acute phase reactants (alpha 1-antitrypsin, alpha 2-macroglobulin, transferrin, alpha 1-acid glycoprotein, C-reactive protein, ceruloplasmin, haptoglobin and the third component of complement) and immunoglobulin in the IgG, IgM and IgA classes were assayed, by laser nephelometry, in sera from 107 East Japanese patients with smear-positive pulmonary tuberculosis and 144 healthy subjects. These levels were correlated with clinical, haematological and radiological features, the levels of antibody to Mycobacterium tuberculosis, and the diameters of the tuberculin skin test read at various times. Levels of all acute phase reactants increased significantly in tuberculosis except for that of transferrin which was lowered. The correlations between the various acute phase reactants in health and disease were calculated. In general, the correlations were lower in disease than in health, except for the third component of complement and a greatly increased correlation between the levels of alpha 1-antitrypsin and ceruloplasmin. There was a significant correlation between levels of some of the acute phase reactants and those of antibodies to M. tuberculosis, mainly with IgG, less with IgA and least with IgM antibodies. By contrast correlations between acute phase protein and total immunoglobulin levels were most evident in the IgM class, less with IgA and not at all with IgG. Although there were some associations between protein levels and age, sex and weight of controls and patients, these were not great enough to account for the differences between the two groups. There was a tendency for patients, but not controls, with intestinal helminthiasis to have higher levels of total IgM than those without evidence of parasites. In general, the levels of proteins bore very little relation to the clinical and radiological features of disease and were, with the exception of the antimycobacterial antibodies, of no diagnostic value. Likewise, protein levels were not associated with the extent of disease; better correlations were found with the ESR and leucocyte count. Transferrin levels tended to be higher in those with chronic disease and showed a correlation with the diameters of the dermal reactions to tuberculin at 24 hours, which were also significantly larger in chronic disease. Among the haematological findings, the most significant was a negative correlation between the lymphocyte count and haptoglobin levels in disease, suggesting a possible regulatory role for this protein.     

Inflammation-induced recombinant protein expression in vivo using promoters from acute-phase protein genes.

We report that promoters for two murine acute-phase protein (APP) genes, complement factor 3 (C3) and serum amyloid A3 (SAA3), can increase recombinant protein expression in response to inflammatory stimuli in vivo. To deliver APP promoter-luciferase reporter gene constructs to the liver, where most endogenous APP synthesis occurs, we introduced them into a nonreplicating adenovirus vector and injected the purified viruses intravenously into mice. When compared with the low levels of basal luciferase expression observed prior to inflammatory challenge, markedly increased expression from the C3 promoter was detected in liver in response to both lipopolysaccharide (LPS) and turpentine, and lower-level inducible expression was also found in lung. In contrast, expression from the SAA3 promoter was found only in liver and was much more responsive to LPS than to turpentine. After LPS challenge, hepatic luciferase expression increased rapidly and in proportion to the LPS dose. Use of cytokine-inducible promoters in gene transfer vectors may make it possible to produce antiinflammatory proteins in vivo in direct relationship to the intensity and duration of an individual's inflammatory response. By providing endogenously controlled production of recombinant antiinflammatory proteins, this approach might limit the severity of the inflammatory response without interfering with the beneficial components of host defense and immunity.     

干扰素、病毒唑+胸腺肽治疗肾综合征出血热的免疫基础研究

肾综合征出血热 (HFRS)在我国发病率最高 ,其发病机制既有病毒的直接作用也与机体免疫功能紊乱有关。当前常应用干扰素、病毒唑 +胸腺肽进行病原治疗 ,但其对患者免疫功能的具体影响尚不清楚。本文对干扰素、病毒唑 +胸腺肽治疗HFRS患者后的细胞免疫功能及细胞因子变化进行了初步研究。1　材料和方法1 1　研究对象　均为 1999年 3月～ 2 0 0 2年 2月收治的HFRS住院病人 ,共 90例。其中男 5 4例 ,女 36例 ;年龄最小14岁 ,最大 6 9岁 ,平均年龄 36 7岁。诊断均经血清学特异性抗体证实 ,均符合 1996年全国HFRS专题会议制定的诊断标准。…     

多学科医疗团队干预与老年急性期患者疾病恢复的相关性

目的评估多学科医疗团队参与管理老年急性期疾病的疗效及预后。方法纳入2017年9月至2018年3月宜宾市第二人民医院老年医学科合并老年综合征并进行多学科干预的122例老年急性期患者作为研究组,选取2016年10月至2017年4月同科室合并老年综合征的140例老年急性期患者作为对照组。比较2组患者住院期间用药情况、患者满意度、住院费用、出院时Barthel日常生活能力(ADL)评分、住院天数、全因死亡率等结局指标。采用SPSS 22. 0软件进行统计学分析。组间比较采用t检验或卡方检验。结果与对照组比较,研究组患者的用药数量[(8. 28±0. 18) vs (9. 33±0. 22),P 0. 05]及住院天数[(10. 42±0. 51) vs (11. 21±0. 73)d,P 0. 05]减少,患者满意度[(97. 56±4. 19) vs (91. 22±3. 71)分;P 0. 05]升高,患者住院费用[(7187. 55±17. 24) vs (7469. 34±22. 18) RMB$,P 0. 05]下降,Barthel ADL评分[(83. 36±4. 29) vs(63. 77±5. 36),P 0. 05]增高,全因死亡率下降(1. 64%vs 4. 63%,P 0. 05)。结论在老年病房中,多学科医疗团队的医疗干预可使老年患者出院时ADL更加独立,住院时间更短且花费更低,死亡率下降,同时患者及其家属的满意度均更高。