抗精子抗体对人精子表面ConA受体影响的研究

本研究运用刀豆蛋白-A(ConA)-胶体金分子探针,对经兔抗人精子抗血清处理的正常人精子和免疫性不育患者的精子做分子水平的定位观察。结果表明,经兔抗人精子抗血清处理的精子和免疫性不育症患者的精子,其顶体反应的囊泡上和顶体后区胶体金的结合均明显低于正常,提示抗精子抗体能封闭精子顶体后区与卵细胞识别的位点,从而影响精子的受精能力。     

活性钙改善不良者精子质量的临床观察

本研究应用活性钙治疗精子质量异常的男性不育者，治疗前后采用精液常规检查，多次曝光法测定精子前向运动速度、底物膜法测定精子顶体蛋白酶活性，以及血清钙，精浆钙浓度等，并按照随机分组及双盲的原则对精子质量进行对照观察研究。     

抗精子抗体对精子顶体酶活性的影响

本文旨在观察抗精子抗体对精子顶体酶活性的影响。选择男性不育者５０例与正常生育者２０例，采用固相酶染色法测抗精子抗体，以固定明胶薄膜法测精子顶体酶活性。结果发现５０例不育者抗精子抗体阳性率为５２％，不育者精子顶体酶活性明显低于生育者；抗精子抗体阳性者顶体酶活性明显低于阴性者，表明抗精子抗体可降低精子顶体酶活性。     

抗精子抗体对精子顶体酶活性的影响

本文旨在观察抗精子抗体对精子顶体酶活性的影响。选择男性不育者50例与正常生育者20例，采用固相酶染色法测抗精子抗体，以固定明胶薄膜法测精子顶体酶活性。结果发现50例不育者抗精子抗体阳性率为52％。不育者精子顶体酶活性明显低于生育者；抗精子抗体阳性者须体酶活性明显低于阴性者，表明抗精子抗体可降低精子顶体酶活性。     

抗精子抗体对精子顶体酶活性的影响

目的：观察抗精子抗体对精子顶体酶活性的影响。方法：选择男性不育者５０例，与正常生育者２０例。采用固相酶染色法测抗精子抗体，固定明胶薄膜法测精子顶体酶活性。结果：５０例不育者抗精子抗体阳性率为５２％。不育者精子顶体酶活性明显低于生育者；抗精子抗体阳性者顶体酶活性低于阴性者。结论：抗精子抗体可降低精子顶体酶活性。     

IgA抗精子抗体对精子顶体反应的影响

目的 探讨精浆中IgA抗精子抗体对人精子顶体反应的影响。方法 利用免疫珠法（IBT）筛选出IgA抗精子抗体阳性精浆标本同正常人精子孵育，以孕酮诱发精子顶体反应；以特异性荧光标记物．络合异硫氰酸荧光素的花生凝集素（FITC-PNA）标记精子顶体，通过流式细胞仪检测精子顶体完整性。结果 与IgA抗精子抗体阳性精浆孵育的精子，其孕酮诱发的顶体反应发生率明显低于正常精浆及精子培养液组（P〈0．01），正常精浆组及精子培养液组间无显著性差异（P〉0．05）；IgA抗精子抗体阳性精浆组、正常精浆组、精子培养液组自发顶体反应的发生率无显著性差异（P〉0．05）。结论 免疫性不育患者精浆中的IgA抗精子抗体可以明显抑制孕酮诱发的顶体反应的发生，可能是导致不育的原因之一。     

人类抗精子抗体对精子功能的影响——1.人类抗精子抗体对精子穿越宫颈粘液的影响

为探讨抗精子抗体对精子穿越宫颈粘液能力的影响,本文用15份含高滴度抗精子抗体的血清配成不同稀释度的血清标本,用间接精子免疫珠结合试验测定各血清标本的抗体滴度,并用精子宫颈粘液穿透试验检测不同滴度抗精子抗体对精子穿越宫颈粘液能力的影响。结果显示:(1)IgA和IgG类抗精子抗体对精子穿越宫颈粘液的能力均有明显影响(P<0.01),且IgA类较IgG类作用大。(2)头部及全身结合有抗体的精子受损较明显,而仅尾部结合有抗体的精子则无明显受损。(3)当结合抗体的活动精子≥60％或头及全身结合抗体的活动精子≥30％时这种影响变得十分确切。     

抗精子抗体对精子顶体酶活性的影响

目的；观察抗精子抗体对精子顶体酶活性的影响。方法：选择男性不育者５０例，与正常生育者２０例。采用固相酶染色法测搞精子抗体，固定明胶薄膜法测精子顶体酶活性。结果：５０例不育者抗精子抗体阳性率为５２５，不育者精子顶体酶生明显低于生育者；抗精子抗体阳怀者顶体酶活性低于阴性者。结论：抗精子抗体可降低精子顶体酶活性。     

精子功能检测的研究——Ⅰ.底物膜技术对精子顶体酶的测定

本研究应用健康人和不育症患者的精液各30人份,用底物膜技术检测精子顶体酶,同时进行精子形态学和多次曝光摄片分析。结果表明,在精子头部周围,顶体蛋白酶和透明质酸酶活性区域呈现亮区或晕。不育症患者顶体蛋白酶反应率和反应区的平均直径显著降低(P<0.01),但是透明质酸酶无明显变化(P>0.05).不育症患者精子畸形率高于健康人.不育症患者和健康人精子运动平均速度相似,但直线运动方式的精子在前者明显减少.本文对精子的顶体酶、形态异常和运动等相互关系作了讨论.     

顶体蛋白酶抑制剂KF-950对精子顶体蛋白酶的抑制作用

本文研究蛋白酶抑制剂KF-950对人精子顶体蛋白酶的抑制作用.醋酸抽提并纯化人精子顶体蛋白酶,BAEE/ADH法测定不同浓度KF-950作用后残余酶的活性.结果正常人精子顶体蛋白酶活性为37.65±4.47mu/ml(pH 8.5,25℃);在加入不同浓度KF-950后,残余酶的活性与KF-950的浓度呈负相关(r=-0.998),1 μgKF-950可抑制1.895 mu人顶体蛋白酶活性(pH 8.5,25℃).结论KF-950对人精子顶体蛋白酶具有很强的抑制作用.     

不育患者精浆α-1,4糖苷酶活性与精液参数的关系

目的　探讨精浆 α-1 ,4糖苷酶活性与精液参数之间的关系。　方法　分光光度比色法测定精浆 α-1 ,4糖苷酶活性及进行精液常规分析。　结果　 2 90 2例男性不育者精浆α-1 ,4糖苷酶活性异常率为 3 8.87%。该酶活性与精子密度、精子活率、a,b级精子活力和顶体酶活性呈显著正相关 ( r分别为 0 .460、0 .1 2 2、0 .0 86和 0 .2 3 0 ,P均 <0 .0 0 1 ) ,而与精液量、精液 p H、液化时间和畸形精子率无显著相关 ( P>0 .0 5)。 α-1 ,4糖苷酶活性正常组精子密度、活率、a,b级精子活力和精子顶体酶活性均明显高于 α-1 ,4糖苷酶活性异常组 ( P<0 .0 0 1 )。以常规精液分析法 ( RSA)主要参数正常与否分成的两组间α-1 ,4糖苷酶活性差异有显著性 ( P<0 .0 0 1 )。　结论　α-1 ,4糖苷酶活性对精子密度、活率、a,b级精子活力和顶体酶活性均有明显影响 ,对精液量、精液 p H、液化时间和畸形精子率无显著影响     

男性年龄与精子顶体酶活性及精子DNA碎片指数的相关性分析

目的探讨男性年龄与精子顶体酶活性、精子DNA碎片指数(DFI)的相关性。方法选取2016年1~8月在我院生殖医学中心就诊的436例不育症男性患者为研究对象,所有患者均行精液常规检查、精子顶体酶活性检查和(或)精子DFI分析。将患者按年龄分为<30岁、30~39岁、≥40岁3组,分析各组的精液常规、顶体酶活性及精子核DFI的差异及其相关性。结果不同年龄段患者的体重指数(BMI)、禁欲天数、精液量无显著性差异(P>0.05);年龄≥40岁组患者的前向运动精子百分率、活动精子百分率及精子顶体酶活性显著低于<30岁和30~39岁组(P<0.05);≥40岁组患者的精子DFI显著高于<30岁和30~39岁组(P<0.05)。年龄与前向运动精子百分率及活动精子百分率之间呈负相关(P<0.05),但是相关性较弱。精子顶体酶活性与精子正常形态率、前向运动精子百分率、非前向运动精子百分率、活动精子百分率呈正相关(P<0.05);精子DFI与年龄、禁欲天数、前向运动精子百分率呈正相关(P<0.01),与精液量、精子浓度、活动精子百分率呈负相关(P<0.05);精子顶体酶活性和DFI之间无相关性(P>0.05)。结论年龄增长会导致精液前向运动精子百分率、活动精子百分率、精子顶体酶活性、DFI等参数改变,直接或间接影响男性生育力。说明年龄对男性不育的影响是多方面的,建议有生育需求的大龄(≥40岁)男性尽早进行生育咨询与评估。     

优选前后精子顶体酶活性与IVF受精率相关性的研究

目的探讨优选处理前和处理后精子顶体酶活性的变化,及与体外受精(IVF)受精率的相关性。方法采用分光光度比色法,对接受IVF治疗的53例不育夫妇男方精液,分别测定优选处理前和处理后精子顶体酶活性,分析其与IVF受精率的相关性。结果优选处理后精子顶体酶活性与优选前比较有显著性差异(P<0.05);达到常规IVF标准并选择常规IVF治疗者,优选后的精子顶体酶活性与IVF受精率有相关性,精子顶体酶活性降低与IVF受精率降低有关。结论精子顶体酶活性与IVF受精率有相关性,并且通过精子顶体酶活性可以预测IVF受精率。     

γ-氨基丁酸对精子顶体酶活性的影响

目的 :研究γ 氨基丁酸 (GABA)对生育男性精子及抗精子抗体 (AsAb)阳性病人的精子顶体酶活性的影响。　方法 :用BAEE ADH联合法同时测定GABA对精子顶体酶活性的影响。　结果 :GABA可明显提高正常及AsAb阳性精子顶体酶的活性 (P <0 .0 1) ;并使精子的Na+ K+ ATPase、Ca2 + ATPase、超氧化物歧化酶 (SOD)的活性增加(P <0 .0 1,P <0 .0 5 ,P <0 .0 0 1)。　结论 :GABA对精子顶体酶活性有显著影响     

Assessment of released acrosin activity as a measurement of the sperm acrosome reaction

Aim： To develop a method for assessing sperm function by measuring released acrosin activity during the acrosome reaction （AR）. Methods： Human semen samples were obtained from 24 healthy donors with proven fertility after 3-7 days of sexual abstinence. After collection, samples were liquefied for 30 min at room temperature. Standard semen parameters were evaluated according to World Health Organization （WHO） criteria. Calcium ionophore A23187 and progesterone （P4） were used to stimulate the sperm to undergo AR. After treatment, sperm were incubated with the supravital dye Hoechst33258, fixed in a glutaraldehyde-phosphate-buffered saline solution, and the acrosomal status was determined by fluorescence microscopy with fluorescein isothiocyanate-labeled Pisum sativum agglutinin （FITC-PSA）. The percentage of sperm undergoing AR （AR%） was compared to sperm acrosin activities as assessed by spectrocolorimetry. The correlation between AR% and acrosin activity was determined by statistical analysis. Results： The AR% and released acrosin activity were both markedly increased with A23187 and P4 stimulation. Sperm motility and viability were significantly higher after stimulation with P4 versus stimulation with A23187 （P 〈 0.001）. There was a significant positive correlation between released acrosin activity and AR% determined by FITC-PSA staining （r= 0.916, P 〈 0.001）. Conclusion： Spectrocolorimetric measurement of released acrosin activity might serve as a reasonable alternative method to evaluate AR. （Asian J Androl 2008 Mar, 10： 236-242）     

精子DNA损伤、核蛋白组型转换与顶体酶活性及精液参数的相关性分析

目的:探讨精子DNA损伤、精子核蛋白组型转换与顶体酶活性及精液参数的相关性。方法:收集535例精液标本,采用精子染色质扩散(sperm chromatin dispersion,SCD)检测精子DNA损伤,并与精子核蛋白组型转换、顶体酶活性和WHO手册(第4版)精液参数进行相关性分析。结果:精子DNA损伤与精子核蛋白组型转换、顶体酶活性、精子浓度及前向运动精子这些指标之间比较均具有显著性差异(P<0.01);精子DNA损伤与年龄、核蛋白组型转换、精子浓度和D级精子比例之间呈显著正相关(P<0.01或P<0.05),而与顶体酶活性呈显著负相关(P<0.001),多元逐步回归分析显示年龄、精子浓度、D级精子比例、核蛋白组型转换及顶体酶活性是5个独立的相关变量。精子核蛋白组型转换、顶体酶活性、精子密度和前向运动精子这4个指标的异常率在精子DNA异常组(DFI≥30%)中均显著的高于正常组(DFI<30%)。结论:精子DNA损伤与精子核蛋白组型转换、顶体酶活性及WHO(第4版)精液各参数之间存在密切的联系,可能是评价精子质量的另一项重要的指标。     

IL-6对人精子顶体反应影响机制的初步探讨

目的:探讨IL-6对人精子顶体反应(AR)的影响机制。方法:采用BAEE/ADH法测定精子顶体酶的活性,以及通过FITC-PSA法检测精子顶体反应。结果:IL-6可诱导精子顶体酶及超氧化物歧化酶(SOD)的活性,促进精子顶体反应;胞外Ca2+单独不能诱导精子顶体反应,且没有胞外Ca2+的参与,IL-6也不能诱导精子顶体反应;蛋白激酶C(PKC)抑制剂calphC能逆转IL-6诱导的精子顶体反应。结论:IL-6对精子顶体反应有一定的促进作用,可能通过诱导精子的顶体酶和SOD活性等途径来实现,在此作用中,也涉及了PKC的激活,且还需要外源性Ca2+的参与。     

Cytochrome P450‐2D6*4 polymorphism seminal relationship in infertile men

This study aimed to assess cytochrome (CY) P450‐2D6*4 polymorphism relationship with semen variables in infertile men. In all, 308 men were included; fertile normozoospermia (N) (n = 77), asthenozoospermia (A) (n = 70), asthenoteratozoospermia (AT) (n = 75) and oligoasthenoteratozoospermia (OAT) (n = 86). They were subjected to history taking, clinical examination, semen analysis, sperm acrosin activity, seminal malondialdehyde (MDA) and CYP450‐2D6*4 genotyping. CYP450‐2D6*4 wild‐type allele was represented in 76.5% of N, 70% of A, 66.7% of AT and 57.7% of OAT men where homozygous gene mutation was present in 5.9% of N, 20% of A, 26.6% of AT and 26.9% of OAT men, respectively. Sperm acrosin activity, sperm concentration, sperm motility, linear sperm velocity and sperm normal forms were significantly higher, and seminal MDA level was significantly lower in men with CYP450‐2D6*4 wild‐type allele compared with men with homozygous mutation. It is concluded that CYP450‐2D6*4 wild‐type allele has higher frequency where homozygous‐type allele has lower frequency in N men compared with A, AT and OAT men. Sperm acrosin activity index, sperm concentration, sperm motility, linear sperm velocity and sperm normal forms were significantly higher, and seminal MDA level was significantly lower in men with CYP450‐2D6*4 wild‐type allele compared with men with homozygous mutation.     

Problems in evaluating male fertility: valuable factors in evaluating male fertility and normal values of seminal parameters

To determine the valuable factor for evaluating male fertility, a comparative study was done as to various seminal parameters between fertile and infertile groups. The fertile group consists of 57 proven fertile males and the infertile group consists of randomly chosen 67 infertile patients. Seminal parameters assessed were sperm concentration, motility, mean velocity, total sperm output, total motile sperm output, sperm morphology, acrosin activity and sperm penetration rate on zona-free hamster egg penetration assay (SPA). The infertile group was significantly different from the fertile group in every parameter except acrosin activity. However, the range of each parameter in the two groups overlapped each other. The diagnostic rate of each parameter, which is the percentage of an infertile male correctly diagnosed as infertile, was calculated by using 95% specificity threshold value of fertile males. The 95% specificity threshold values of sperm concentration, motility and % normal shaped sperm were 24.9 x 10(6)/ml, 34.9% and 55%, respectively, and they could be acceptable for the normal limit of seminal parameters. The diagnostic rate was highest in penetration rate (72.4%). In other words, penetration rate is the most valuable factor in various parameters for making a distinction between fertile and infertile males. Sperm motility and mean velocity showed the next highest diagnostic rate. On the other hand, sperm concentration showed a poor diagnostic rate (36.8%). In addition, there was no significant correlation between penetration rate and any other seminal parameters. These results suggest that the SPA will be an essential test for evaluating male fertility and penetration rate may be a marker of male fertility in the treatment of male infertility.     

精浆免疫抑制物质与抗精子抗体等参数的关系

本文报告了精浆免疫抑制物质(SPIM)活性与精子活率、密度、精子顶体酶活力、精浆果糖和血清抗精子抗体阳性率的比较研究。结果表明SPIM活性与精子活率、密度呈正相关;不育组、流产组的SPIM活性与ASA阳性率和精子顶体酶活率无显著差异(P>0.05);而SPIM活性、精子顶体酶活力均较生育组低,ASA阳性率均较生育组高(P<0.05～P<0.01),生育、不育和流产组之间果糖浓度均有显著差异(P<0.05～P<0.01)。