HPLC法测定盐酸氯己定痱子粉中盐酸氯己定的含量

目的　建立测定盐酸氯己定痱子粉中盐酸氯己定的高效液相色谱分析方法。方法　采用 Kromasil C18(15 0 mm× 4 .6 mm,5 μm)色谱柱 ,0 .0 12 mol· L-1庚烷磺酸钠的甲醇溶液 -水 -冰醋酸 (73∶ 2 7∶ 12 )为流动相 ,流速 1.0 m L· min-1,检测波长 2 5 9nm。结果　线性范围 4 .188～ 2 0 .94 mg· L-1(r =0 .9998) ,平均回收率为 97.4 % ,RSD为 0 .5 2 %。结论　该法简便、快速、准确 ,适用于盐酸氯己定痱子粉中盐酸氯己定的含量测定。     

HPLC法测定甲硝唑氯己定洗剂中氯己定含量

目的:建立HPLC法测定甲硝唑氯己定洗剂中氯己定含量的方法。方法:采用AGT XBP-C_8(250mm×4.6mm,5μm)柱,以甲醇-0.1%三乙胺溶液(含庚烷磺酸钠10mmol·L~(-1),用磷酸调pH至3.0)(68:32)为流动相,检测波长为260nm。结果:氯己定在进样量1.998～9.992μg范围内呈良好的线性关系(r=0.999 9),回收率为99.5%,RSD为1.9%(n=9)。结论:本方法简便、快速准确,可作为该制剂中葡萄糖酸氯己定质量控制方法。     

HPLC法测定盐酸氯己定的有关物质和含量

目的:建立高效液相色谱法测定盐酸氯己定有关物质和含量的方法.方法:采用资生堂MG C18(250 mm×4.6 mm,5μm)色谱柱;以辛烷磺酸钠溶液(取辛烷磺酸钠2.0 g,加冰醋酸120 ml,水270 ml)-乙腈(60:40)为流动相;流速:1.0 ml·min-1;检测波长:254 nm;柱温:35℃;进样量...     

二阶导数光谱法测定复方异丙嗪糖浆中盐酸异丙嗪的含量

目的用紫外分光光度法不经分离直接测定复方异丙嗪糖浆中盐酸异丙嗪的含量。方法二阶导数光谱法,测定波长为256nm。结果盐酸异丙嗪在5．21～31．26ug/ml浓度范围内线性关系良好,峰零间振幅D对浓度C的回归方程C=-651．25×D＋0．3256,r=1．0000,平均回收率为101．10％,相对标准偏差为0．98％。结论二阶导数光谱法测定复方异丙嗪糖浆中盐酸异丙嗪含量简便、快速、结果准确。     

二阶导数光谱法测定克霉唑软膏的含量

用二阶导数光谱法不经预处理直接测定克霉唑软膏中克霉唑的含量。该法操作简便,结果准确,平均回收率为99.46,RSD=0.48％。     

导数光谱法测定盐酸利多卡因注射液的含量

盐酸利多卡因为临床上常用的抗心律失常药和局麻药,其含量测定中国药典85版采用提取一中和法,此法操作费工费时,很不适于医院药房制剂的快速分析。盐酸利多卡因经扫描在紫外区虽然有吸收(见图1),但吸     

醋酸氯己定栓剂中醋酸氯己定的含量测定

目的建立醋酸氯己定栓剂中醋酸氯己定的含量测定方法。方法采用紫外分光光度法测定醋酸氯己定的含量。结果醋酸氯己定在2～10μg/mL浓度范围内线性关系良好,其回归方程为:C=13.35A+0.06273(r=0.9997),平均回收率99.6%,RSD=0.83%(n=9)。结论此含量测定方法简便,准确可靠。     

二阶导数光谱法测定茶碱缓释片含量

目的 :不经分离直接测定茶碱缓释片含量。方法 :二阶导数光谱法。结果 :茶碱在 2 .5～ 12 .5 μg ml浓度范围内线性关系良好 ,r =0 .9998平均回收率 10 0 .0 8% ,RSD 1.84(n =5 ) ,测得茶碱含量符合中国药典规定。结论 :该方法测定茶碱缓释片含量准确、快捷 ,与中国药典该项下化学滴定法测定结果基本一致。     

二阶导数光谱法测定苯海拉明麻黄碱滴鼻液的含量

本文报道用二阶导数光谱法可以分别测定苯海拉明麻黄碱滴鼻液中盐酸苯海拉明和盐酸麻黄碱的含量。实验结果证明：本法简便、正确、重现性好。     

一阶导数紫外分光光度法测定瑞巴派特片中瑞巴派特的含量

瑞巴派特是一种新型胃粘膜保护剂 ,具有预防溃疡发生和促进溃疡愈合的作用 ,可增加胃粘膜血流量、前列腺素 E2的合成和胃粘液分泌 ,并促进消化性溃疡的愈合及炎症的改善。新药暂行标准 [1 ] 采用以二甲基甲酰胺作溶剂的碱量法测定含量 ,文献报道主要有高效液相色谱法 [2 ] 。本文建立了以p H6 .8的磷酸盐缓冲液作溶剂的一阶导数紫外分光光度法测定瑞巴派特片含量的方法 ,可消除片剂中全辅料的干扰。结果表明 ,本法与碱量法测定结果一致。本法操作简便、省时 ,专属性强 ,结果满意。1　仪器与试药1.1　仪器　 U V- 2 5 6 FW型紫外可见分光…     

二阶导数光谱法测定培氟沙星片的含量

以二阶导数光谱法测定培氟沙星片的含量，以峰－谷间（２９２～２７８ｎｍ）的振幅值为定量依据，可以消除辅料对测定的干扰。平均回收率为１００．１％，ＲＳＤ为０．８％（ｎ＝６）。对三个批号的样品进行了测定，方法简便、准确、快速。     

二阶导数光谱法测定葛根芩连微丸的含量

目的：不经分离直接测定葛根芩连微丸的含量。方法：二阶导数光谱法，盐酸小檗碱在２～１０μｇ·ｍｌ－１浓度范围内线性关系良好，峰零间振幅Ｌ对浓度Ｃ的回归方程Ｃ＝２８１．６９Ｌ＋０．２５３５，ｒ＝０．９９８６。结果：平均回收率为１００．３７％，ＲＳＤ为０．８７％（ｎ＝５），测得样品含量符合药典规定。结论：本文以二阶导数光谱法测定葛根芩连微丸的含量，准确、满意，较之薄层扫描法、碘量法等更为快捷、方便     

一阶导数光谱法测定解磷注射液中氯磷定的含量

目的：建立解磷注射液的含量测定方法。方法：采用一阶导数光谱法不经分离直接测定解磷注射液的含量。选用氯磷定的测定波长为２７９ｎｍ。结果：相关系数为ｒ＝０．９９９８,平均回收率为９９．８２％,ＲＳＤ为１．０３％。结论：以一阶导数光谱法测定氯磷定的含量,结果准确、满意,较紫外分光光度法更为快捷、方便。     

一阶导数光谱法测定奈韦拉平片含量

目的:建立测定奈韦拉平片中奈韦拉平含量的方法。方法:采用一阶导数UV光谱法,以275 nm(峰)与299 nm(谷)振幅值为定量依据,进行定量分析。该法与RP-HPLC法和零阶导数光谱法进行了对比。色谱柱为Shimadzu ODS(250 mm×4.6 mm,5μm),流动相为甲醇-水(用磷酸调pH=3),(60∶40),流速为1.0 mL.min-1;紫外检测波长282 nm,柱温35℃,采用外标法计算含量。结果:结果表明奈韦拉平浓度在2.5～25.0μg.mL-1范围内与振幅值呈良好的线性关系(r=0.9997),平均回收率为99.5%(RSD=1.5%)。统计检验发现HPLC法与一阶导数UV法无显著性差异,而与零阶导数法有显著性差异。结论:一阶导数UV光谱法可靠,简便快速,可有效消除赋形剂干扰,结果准确,可用于奈韦拉平片的质量控制。     

荧光分光光度法测定盐酸环丙沙星片的含量

采用荧光分光光度法测定盐酸环丙沙星片的含量简便、快速、灵敏度高。线性范围为０．１～０．５μｇ／ｍｌ，平均回收率为１００．０４％，相对标准差为０．６０％。     

一阶导数紫外光谱法测定盐酸芬氟拉明片的含量及含量均匀度

目的：建立一阶导数紫外光谱法测定盐酸芬氟拉明片的含量及含量均匀度,以增加质量控制方法。方法：用水作溶剂,在２８０与２２０ｎｍ波长范围内测定一阶导数光谱,以一阶导数光谱在２６５ｎｍ与２６９ｎｍ波长处峰－谷间的振幅Ｄ为定量依据,用对照品对照法计算含量及含量均匀度。结果：盐酸芬氟拉明浓度在０．０５～０．４ｍｇ／ｍｌ之间与－阶导数峰－谷间的振幅呈良好的线性关系,相关系数ｒ－０．９９９９,６次的平均加样回收     

二阶导数光谱法测定酮基布洛芬栓的含量

本文采用二阶导数光谱法测定酮基布洛芬栓的含量,以255±1nm处为测定波长,排除了基质的干扰,具简便快速。平均回收率为99.80%,CV=1.51%     

Determination of nimesulide in pharmaceutical dosage forms by second order derivative UV spectrophotometry

In this study, nimesulide which has been used as an analgesic, antipyretic and anti-inflammatory agent, was analyzed by using second order derivative UV spectrophotometry. The solvent, the degree of derivation, ranges of wavelength and n-value were chosen in order to optimize the conditions. The concentration of nimesulide in its solutions in ethanol and chloroform were determined between the wavelength ranges of 200 and 500 nm (n = 6, delta lambda = 21) and in the linearity ranges of 2.0-90.0 microg ml(-1) in ethanol and 2.0-50.0 microg ml(-1) in chloroform by using the values obtained from the second derivative UV spectrum of the substance. The developed second derivative UV spectrophotometric method was applied to the pharmaceutical preparations such as tablet, sachet (granule) and suspension. Tablet and sachet were analysed in ethanol while the suspension was analysed in chloroform. The results obtained from derivative UV spectrophotometry were compared with those obtained by using HPLC. It was found that the difference was not statistically important between these methods. It was concluded that developed derivative UV spectrophotometric method was accurate, sensitive, precise, reproducible and could be applied directly and easily to the pharmaceutical preparations.     

Simultaneous determination of benazepril hydrochloride and hydrochlorothiazide in tablets by second-order derivative spectrophotometry

A second-order derivative spectrophotometric method for the simultaneous determination of benazepril hydrochloride and hydrochlorothiazide in pharmaceutical dosage forms is described. The determination of benazepril hydrochloride in the presence of hydrochlorothiazide was achieved by measuring the second-order derivative signals at 253.6 and 282.6 nm, while the second-order derivative signal at 282.6 nm was measured for the determination of hydrochlorothiazide. The linear dynamic ranges were 14.80-33.80 microg ml(-1) for benazepril hydrochloride and 18.50-42.20 microg ml(-1) for hydrochlorothiazide, the correlation coefficient for the calibration graphs were better than 0.9998, n = 5, the precision (%RSD) was better than 1.43% and the accuracy was satisfactory (Er < 0.99%). The detection limits were found to be 2.46 and 1.57 microg ml(-1) for benazepril hydrochloride and hydrochlorothiazide, respectively. The method was applied in the quality control of commercial tablets and proved to be suitable for rapid and reliable quality control.