荨麻疹患者血清特异性IgE过敏原检测结果排序分析

目的 了解荨麻疹患者血清特异性IgE检测过敏原的情况.方法 回顾国内核心期刊发表的关于荨麻疹患者通过血清特异性IgE检测过敏原的文献报道,归纳总结引起人们过敏的过敏原主要为哪些.结果 吸入性过敏原以尘螨、粉螨最高,约占19.14 %.食入性以虾最高,约占11.03 %.结论 荨麻疹病因比较复杂,过敏原是大多数荨麻疹患者发病的主要因素,通过了解人们对过敏原过敏情况,为临床治疗和预防提供依据.     

2972例变应性疾病患者皮肤点刺试验临床分析

目的:了解温州地区常见变应性疾病经皮肤点刺试验诊断过敏原的分布,探讨常见变应性疾病与过敏原的关系。方法:使用丹麦爱尔开-阿贝优公司的吸入组点刺试剂和德国默克公司的食入组点刺试剂,组胺液为阳性对照,生理盐水为阴性对照。结果:①2972例患者吸入过敏原以粉尘螨和屋尘螨阳性例数居多,其次为霉菌1;食入过敏原以小虾阳性例数最多。②2972例患者中变应原阳性率以男性居多。③1岁～18岁患者吸入过敏原与食入过敏原阳性率均呈上升趋势。结论:温州地区常见变应性疾病皮肤点刺试验有较高的检出率,其中吸入过敏原以粉尘螨和屋尘螨为主,食入过敏原以小虾为主。     

过敏性紫癜儿童血清过敏原特异性IgE抗体检测分析及与空气质量关系的初步研究

目的：分析过敏性紫癜（HSP）患儿血清过敏原谱分布并探讨其发病及其与空气质量的关系。方法：以2019年11月至2021年10月于天津市儿童医院住院的116例HSP患儿为研究对象;采用酶联免疫捕获法定量检测血清过敏原特异性IgE抗体水平,分析HSP患儿血清过敏原谱分布;收集同时间段天津市空气质量监测数据,探讨HSP发病及其与空气质量的关系。结果：116例HSP患儿血清中,有特异性IgE抗体检测阳性患者63例,阳性率54.31%,其中一种过敏原阳性35例,阳性率30.17%,两种及两种以上过敏原阳性28例,阳性率24.14%。食入性过敏原中以鸡蛋、牛奶及小麦面粉阳性率最高,分别为31.03%、12.07%和8.62%;吸入性过敏原中以粉尘螨、交链孢霉及屋尘阳性率最高,分别为15.52%、12.93%和8.62%。不同性别HSP患儿总特异性IgE抗体阳性检出率差异无统计学意义（P>0.05）,女性患儿对牛奶过敏的阳性检出率高于男性,差异有统计学意义（P<0.05）。HSP好发于学龄前期儿童,且年龄越小对鸡蛋、交链孢霉过敏的阳性检出率越高,差异均有统计学意义（P<0.05）。...     

通辽地区过敏原检测结果及与其他地区对比分析

为了解通辽地区常见过敏原的分布情况与特点,采用欧蒙免疫印迹法,对428例疑似过敏性疾病患者的血清标本中18种过敏原的特异性IgE类抗体进行半定量检测。根据各种过敏原特异性IgE抗体阳性百分率,分别对通辽地区和其他地区的结果进行了排序,并计算出各地区同一过敏原特异性IgE抗体阳性百分率的平均序位,并将通辽地区序位与其他地区序位和各个地区平均序位进行了对比。结果显示,通辽地区食入性和吸入性过敏原阳性率最高的分别为淡水鱼组合(10.30%)和艾蒿(6.54%)。有20.56%的过敏性疾病患者只对某一种过敏原呈阳性反应,有10.51%的患者对两种过敏原呈阳性反应。通辽地区蟑螂、霉菌组合、屋尘、普通豚草、尘螨组合的特异性IgE抗体阳性百分率的序位明显低于各地区平均序位,而螃蟹/虾、葎草、花生、淡水鱼组合、海洋鱼组合和大豆的序位明显高于各地区平均序位。这提示淡水鱼组合、海洋鱼组合、大豆、花生、艾蒿、树木组合、蟑螂、狗上皮、豚草、尘螨组合是通辽地区常见的引起过敏性疾病的过敏原。多数患者只对一种或两种过敏原呈阳性反应。通辽地区过敏原特异性IgE抗体阳性百分率的序位与其他地区序位和各地区平均序位对比显示,通辽地区疑似过敏性疾病人群的过敏原分布具有鲜明的特征。     

胃息肉患者过敏原测定结果分析

目的 分析检查胃息肉病人血清过敏原特异性IgE抗体（sIgE）的结果。方法 选择我院120例胃息肉患者设为患者组,另选择同期120例健康体检者为对照组,均进行血清sIgE检查,对比两组sIgE检测的阳性率。结果 120例胃息肉患者sIgE抗体检测阳性率高达56.67%（68/120）,而对照阳性率为11.67%（14/120）,两者比较差异有统计学意义（P＜0.05）。在吸入性sIgE抗体检测显示,患者组尘螨组合的sIgE阳性比率高于对照组（P＜0.05）。在食入性过敏原sIgE方面,患者组中海鱼组合、淡水鱼组合的sIgE阳性比率较对照组显著升高（P＜0.05）,患者组中食入性过敏原sIgE阳性比率最高的是蟹,占28.33%。结论 胃息肉患者具有较高的血清过敏原sIgE抗体阳性检出率,过敏因素与胃息肉的发病有一定关联,对胃息肉的预防有一定的指导意义。     

过敏性鼻炎患儿过敏原特异性IgE检测与临床意义

目的 了解过敏性鼻炎儿童主要过敏原分布情况及特点,以指导临床防治.方法 采用免疫印迹法体外定量检测患儿血清中特异性IgE抗体及20种常见过敏原.结果 938例过敏性鼻炎患儿中,100%至少对一种以上的过敏原呈阳性反应,其中487例患儿总IgE〉400IU/Ml,230例患儿总IgE在200IU/ml~400IU/ml之间.在检测20种过敏原中以户尘螨和屋尘的阳性率最高（90.0%,80.8%）.结论 过敏性鼻炎患儿至少有一种特异性IgE抗体显著增加,户尘螨、屋尘、动物皮屑、羊肉和牛奶是引起儿童过敏性鼻炎的主要过敏原.儿童在春秋温暖季节易发过敏性鼻炎.     

儿童特应性皮炎血清特异性IgE和斑贴试验结果的临床分析

目的:回顾性分析广州地区特应性皮炎(AD)患儿常见变应原,为AD预防和诊治提供依据.方法:ELISA检测138例AD患儿血清特异性IgE(sIgE),并分析其吸入组、食入组变应原;其中43例患儿同时接受了用于检测日常接触类变应原的皮肤斑贴试验,分析其结果分布特征.结果:吸入性特异性总IgE抗体检测结果阳性率为57.97...     

慢性荨麻疹患者过敏原特异性IgE抗体检测

慢性荨麻疹（chronic urticaria,CU）是人们常见的一种皮肤病,引起的原因很多;由于不慎食入或接触过过敏原而引起的慢性荨麻疹占有较大比率,我们采用免疫标记法检测70例慢性荨麻疹患者的血清,了解其过敏状况以指导临床预防及治疗,现报道如下。     

免疫球蛋白E在儿童呼吸道变态反应性疾病中的作用及分布

目的探讨呼吸道变态反应性疾病儿童特异性免疫球蛋白IgE（SIgE）在各年龄分布及其与总IgE（TIgE）的关系。方法采用荧光酶联免疫检测系统,对73例哮喘、过敏性咳嗽患儿,按年龄分为3组,进行14项SIgE检测,包括食人组和吸入组,同时检测TIgE。结果①患儿TIgE检出率,三组检出率没有显著差异。②患儿SIgE检出率有随年龄增加而增高的趋势。③患儿〈3岁组以食物多价Fmea以及Dx最高,各为5例;而3—6岁组以Fmea最高,为15例;〉6岁组Mxl最高14例。④SIgE和TIgE检测结果有明显相关,但两者检出率无明显差异。如以SIgE阳性和（或）TIgE阳性合并统计,患儿IgE阳性检出率为95．89％。结论①患儿〈3岁组的SIgE和TIgE检出率并不低。②患儿〈3岁婴幼儿最常见的变应原为食入性Fmea、F2、F4。③3～6岁学龄前儿童仍以食人性变应原为主,最高为Fmea,其次为F2,但吸入性变应原开始增多,Dx、Mxl以及wx;④〉6岁组学龄儿童则以室内吸入性变应原为主,Mxl最高,其次为El／E5、Dx,还有食入性Fmea。⑤SIgE与TIgE有很好的相关性,SIgE联合TIgE检测能够提高检出率,更有效地诊断儿童呼吸道变态反应疾病。     

慢性荨麻疹患者幽门螺杆菌检测的意义

目的：研究慢性荨麻疹与幽门螺杆菌（Helicobacter pylori ,HP）感染的相关性,探讨HP检测在慢性荨麻疹患者诊疗中的意义。方法：选取2014年4月至2015年7月门诊治疗的慢性荨麻疹患者420例,并随机选取同期体检中心健康体检者450例为健康对照组,采用胶体金法检测患者及健康体检者血清HP尿素酶抗体,分析慢性荨麻疹组和健康对照组两组HP的阳性情况;同时将162例慢性荨麻疹HP阳性患者分为实验组88例和对照组74例,对照组患者应用常规荨麻疹治疗,实验组患者在对照组治疗基础上增加抗HP三联疗法,分析HP检测阳性的慢性荨麻疹患者采用不同治疗方法的临床疗效。结果：慢性荨麻疹组HP阳性率为38.6%,健康对照组HP阳性率为14.4%,差异有统计学意义（P<0.05）;HP检测阳性患者实验组临床疗效有效率显著高于对照组的有效率,差异有统计学意义（P<0.05）。结论：慢性荨麻疹与HP感染之间存在密切的相关性, HP检测对慢性荨麻疹患者的诊疗具有重要的临床意义。     

江苏省徐州地区支气管哮喘儿童血清过敏原检测结果分析及临床意义

目的:观察支气管哮喘儿童的过敏原检测结果,探讨过敏原特异性血清特异性免疫球蛋白E(special immunoglobulin E,SIgE)、血清嗜酸粒细胞阳离子蛋白(eosinophil cationic protein,ECP)、总免疫球蛋白E(total immunoglobulin E,TIgE)在支气管哮喘...     

IgE to food allergens are highly prevalent in patients allergic to pollens, with and without symptoms of food allergy

Serum samples from 274 patients allergic to one or more of three pollens (birch, grass, mugwort), from 36 patients allergic to cat and/or Dermatophagoides pteronyssinus but not to pollen and from 55 non-allergic controls, as well as 20 cord blood samples, were examined for specific IgE to six ‘pollen-associated’ food allergens In uiing a new sensitive assay (CAP). A questionnaire asking for reactions to food was also sent to all patients. In the pollen group, 111 patients (47%) were positive (≥0.71 kU/l) fora food allergen (392 positive tests). Of these, 92 were sensitive to apple, 68 to potato, 64 to carrot, 63 to celery, 61 to peach and 44 to melon. In the non-allergic group, no IgE to any of the food allergens tested was found, whereas in the group allergic to non-pollen allergens, only one individual had such an IgE. The CAP assay was found to he more sensitive than RAST for the allergens studied. A history of clinical reactions (oral symptoms in 67, rhinoconjunctivitis in 65, asthma in 42 and urticaria in 39) to the corresponding food allergen was reported mainly by patients with positive CAP. In conclusion, we found a high prevalence of IgE to some food allergens in patients allergic to pollen and Ihe absence of such antibodies in the control groups. The new in vitro assay, being moresensitue than previous ones, indicated a high prevalence of food specific IgE in pollen allergic patients, which in many cases did not correspond to clinical symptoms of food allergy.     

患儿鸡蛋、牛奶过敏原体外检测分析

为了解患儿鸡蛋、牛奶过敏原分布情况,采用荧光ELISA法检测患儿血清中鸡蛋和牛奶过敏原SIgE抗体.结果表明:患儿中鸡蛋SIgE和牛奶SIgE阳性检出率分别为31.1%和21.7%.0～1岁组,1～3岁组和3～6岁组鸡蛋SIgE的阳性率显著高于6～14岁组(P＜0.05 ); 0～1岁组和1～3岁组牛奶SIgE的阳性率显著高于3～6岁组和6～14岁组(P＜0.05), 鸡蛋SIgE和牛奶SIgE阳性率都有随着年龄增长而逐渐降低的趋势;鸡蛋SIgE阳性同时牛奶SIgE阳性患儿的阳性率为40.9%,牛奶SIgE阳性同时鸡蛋SIgE阳性患儿的阳性率为44.3%.支气管肺炎患儿鸡蛋和牛奶SIgE阳性率最高,分别为30.3%和29.5%.建议在变态反应性疾病患儿和支气管肺炎患儿中检测鸡蛋SIgE和牛奶SIgE.     

广州地区支气管哮喘患者常见吸入变应原调查分析

目的　调查广州地区支气管哮喘发病相关的主要致敏变应原 .方法　对 10 2例支气管哮喘缓解期患者 ,其中儿童 4 8例 ,成人 5 4例 ,用尘螨、霉菌、猫毛等 12种常见吸入变应原进行皮肤点刺试验 ,并测定其中 2 7例总IgE(TIgE)的水平 .结果　儿童组尘螨皮试阳性率最高 (均为 79.2 % ) ,其次是屋尘 (72 .9% ) .成年组尘螨皮试阳性率最高 ,粉尘螨和屋尘螨分别为 5 9.3%、6 2 .9% ,其次为屋尘 (4 0 .7% ) .在动物毛发皮屑中 ,以狗毛为主 ,猫毛次之 ;对于所有变应原 ,儿童组的阳性百分率远远高于成年组 ;2 7例血清总IgE的均值为 5 0 6 .1KU/L ,远高于正常人 ,其中皮试阳性者TIgE水平高于皮试阴性者 (p<0 .0 1) .结论　广州地区主要变应原均为尘螨 ,且哮喘儿童对尘螨过敏的阳性率显著高于成人     

Distribution of serum IgE in children and adolescents aged 7 to 16 years in Copenhagen, in relation to factors of importance

The distribution of total serum-IgE and factors of importance for the level of IgE was studied in a random sample of 508 children and adolescents, aged 7-16 years, from Copenhagen. A detailed history about asthma, rhinitis, dermatitis and urticaria was obtained, and a physical examination, skin prick test with 9 common allergens, lung function test, bronchial challenge with inhaled histamine and exercise, and measurement of IgE (kU/l) were performed. The distribution of IgE among children and adolescents was found to exhibit a log normal distribution and a positive skin prick test, allergic symptoms, a family history of allergic diseases, age and smoking were found to be significantly related to an increased level of IgE. No relationship was found between increased bronchial responsiveness and IgE. The geometric mean of "normal" values of IgE (*1 SD and *2 SD) of the Danish children and adolescents was 18 kU/l (*4.7, *18.2), suggesting that normal IgE values were within 330 kU/l. Measurement of IgE as the only screening for allergic disease is unreliable, as the predictive value of an elevated IgE in population samples was found to be 50%, whereas misclassification (1-specificity) of asymptomatic subjects as allergic because of an increased IgE was low (4%). In conclusion, total IgE is highly influenced by allergen skin reactivity. Further, this study suggests that normal IgE values were within 330 kU/l, although the range was wide.     

Prevalence of IgE antibodies specific for food allergens in patients with chronic urticaria of unexplained etiology

We investigated whether some cases of chronic urticaria of unexplained cause might be related to food allergy which had remained undetected during routine examination. This investigation was undertaken as the consequence of the availability of a new in vitro assay for specific IgE with increased sensitivity. The following three groups of subjects were studied: 1) a control group of 60 nonatopic subjects, 2) 60 patients with allergy to perennial aeroallergens without skin involvement, and 3) 60 patients suffering from chronic urticaria with no evidence of any triggering factor despite careful clinical investigation. Specific IgE against 19 food allergens frequently involved in urticaria were investigated in all subjects with the new CAP System (Pharmacia). Positive results (CAP > 0.70 kU/1) for one or more food allergens were found in none of the nonatopic controls, in six of the subjects with respiratory allergy, and in 16 of the urticaria patients. The use of an in vitro test with an increased sensitivity allowed us to detect a significant prevalence of IgE specific for food allergens in patients with chronic urticaria of unknown origin. This suggests that, in several of those patients, chronic urticaria might be triggered by a food allergy undetected by the usual methods.     

南京地区过敏性紫癜患儿血清过敏原特异性IgE结果分析

目的 回顾性分析南京医科大学附属儿童医院过敏性紫癜(Henoch-Schonlein purpura,HSP)患儿的过敏原检测情况,为临床诊治提供参考。方法 采用德国MEDIWISS过敏原体外检测系统,检测本院最近四年内接诊的1026例HSP患儿的血清过敏原特异性免疫球蛋白E(specific immunoglobulin E,sIgE)的含量。结果 432例HSP患儿血清sIgE检出为阳性,阳性率为42.11%(432/1026),该432例HSP患儿血清过敏原sIgE分布以1~2级为主,学龄组患儿血清过敏原sIgE阳性率占总人数的65.74%(284/432),远高于婴幼儿组(27例)和学龄前组(121例)(F值分别为7.71和4.05,P值均<0.05)。结论 患儿体内sIgE水平不同程度的增高对HSP的诊治具有一定意义。控制患儿与过敏原接触是防治HSP的关键。     

An epidemiologic study on prevalence of allergen-specific IgE antibodies in school children living in rural area of Saitama Prefecture, Japan]

BACKGROUND: In order to estimate the sensitization to inhalant allergen in childhood, prevalence of allergen-specific IgE antibodies was retrospectively surveyed on sera from school children aged from 9 to 15 living in rural area of Saitama prefecture, Japan, in 2001 and five years ago from 2001 (1996). METHODS: Allergen-specific IgE antibodies against Japanese cedar pollen (JCP), ragweed, sweet vernal grass, Dermatophagoides pteronyssinus (Dp), house dust (HD) and cat dander were examined on sera from 79 school children aged from 9 to 11 (group A) and 119 school children aged from 12 to 15 (group B) collected in 2001, and sera from 117 school children aged from 9 to 11 and 56 school children aged from 12 to 15 collected in 1996. Furthermore, a questionnaire survey about diagnosis of any allergic diseases and symptoms associated with the diseases was conducted on the same subjects in 2001. RESULTS: On the survey from school children, group A and B, collected in 2001, percentage of positive cases for allergen-specific IgE antibodies against any of 6 allergens were 49 in both of the groups for JCP, 10 and 12 for ragweed, 18 and 19 for sweet vernal grass, 39 in both of the groups for Dp, 42 in both of the groups for HD, 23 and 14 for cat dander, respectively. On the survey from school children collected in 1996, there is no significant difference from the percentage of positive cases for allergen-specific IgE antibodies against each allergen in 2001. Among the subjects followed-up on the survey in both of the years, the percentage of positive cases for allergen-specific IgE antibodies against each of six allergens on the survey in 2001 increased as compared with those in 1996. As a result of questionnaire survey in 2001, percentage (70: group A, 89: group B) of positive cases for allergen-specific IgE antibodies against any 6 allergens in the subjects with any symptoms associated with allergic diseases, but not diagnosed, was significantly higher than the percentage (34: group A, 48: group B) of that in the subjects without any symptoms associated with the diseases. CONCLUSION: Further prospective study is required to clarify a cause of allergic diseases in childhood, and it should be taken precautionary measures during childhood against sensitization with inhalant allergen to prevent allergic diseases in surveyed area.     

Risk factors and characteristics of early-onset asthma in Taiwanese children.

BACKGROUND AND PURPOSE: Early-onset asthma has been reported to be associated with a family history of allergy and exposure to environmental factors. This study was designed to evaluate the relationship between age of onset of asthma and genetic and environmental factors with asthma severity in Taiwanese children. METHODS: A group of 352 children with asthma (220 males and 132 females), ranging in age from 5 to 15 years, were enrolled in this study. The subjects were divided into 2 groups: early-onset asthma (up to and including age 3) and late-onset asthma. General characteristics including family history of allergies and exposure to domestic pets and tobacco smoke were recorded. The subjects underwent pulmonary function testing and analysis of serum immunoglobulin E (IgE), eosinophil counts, and specific IgE for common allergens. RESULTS: Early-onset asthma was present in 149 subjects and late-onset asthma in 203. Family history of allergies included a sibling with asthma or urticaria predisposed to early-onset asthma (asthma, p=0.034; urticaria, p=0.024). Food and milk allergen sensitization were more common in early-onset asthma (food allergens, p=0.025; milk, p=0.034). Children with early-onset asthma had higher eosinophil counts (p=0.041). However, there was no correlation between age at onset and pulmonary function testing, the levels of total IgE and IgE specific for Dermatophagoides pteronyssinus or Dermatophagoides farinae. CONCLUSIONS: A history of asthma or urticaria in a sibling is a risk factor for early-onset asthma. A greater prevalence of food allergen sensitization and high eosinophil counts are characteristic of early-onset disease.