STK15在眼睑鳞状细胞癌中的表达及临床意义

近年的研究表明,细胞有丝分裂的启动与维持除了受熟知的细胞周期素依赖激酶l( cyclin dependent kinase 1,CDK1)调控外,还受到其他的一些激酶,如保罗样激酶1( polo like kinasel,plk1)、丝氨酸/苏氨酸激酶15(serine/threonine kinasel5,STKl5)的调节,STKl5通过磷酸化特定下游底物影响细胞G2/M期转换,促进细胞进入M期后中心体成熟及双极纺锤体建立;STKl5在恶性肿瘤中的过表达与中心体的异常扩增、非整倍体形成及细胞恶性转化密切相关[1-2].本研究应用免疫组织化学法检测STKl5在眼睑鳞状细胞癌(squamous cell carcinoma,SCC)的表达,旨在探讨二者的相关性.     

Ki-67,P53及HPV在眼睑结膜鳞状细胞癌2例的表达

我们对2例患者的肿瘤组织切片，利用免疫组化及多酶链反应（Polymerase Chain Reaction.PCR）方法研究了Ki-67，P53，及人类乳头状瘤病毒（Human Papilloma Virus HPV）在眼睑、结膜鳞状细胞癌的表达报道如下。     

显性结膜鳞状细胞癌1例

患者,男,37岁,以"右眼肿块迅速增大,无痛感5mo"就诊于我科.行眼科常规检查,发现一发白带蒂肿块位于右眼睑裂区角膜缘颞侧.遂行肿块切除术,手术成功.对切除肿块进行病理组织学检查,显示为分化良好的鳞状细胞癌.通过对此例居住在热带地区的亚裔患者发生的结膜鳞状细胞癌的诊治,认为长期的阳光照射是导致鳞状细胞癌发生的一个主要危险因素,提示我们对于任何眼表面病变都必须进行仔细的评估,进而正确诊断和治疗.     

结膜鳞状细胞癌1例

1病例报告女,42岁,左眼球内侧新生物2mo。患者在无意中发现左眼球内侧有一黄豆粒大突起,时有异物感,无视物不清,畏光等症状,2002-08-29以左结膜新生物住院。专科检查:视力:双1.0,左眼球结膜轻度充血,鼻侧角膜缘处球结膜有一灰黄色乳头状肿物,约5mm×5mm×3mm,表面凹凸不平呈杨梅状外观,周围有数条血管围绕。眼B超及胸透检查均未见异常,全身淋巴结无肿大及压痛。入院后2d在局麻下上行肿物切除+游离结膜瓣修补术,术中沿肿物周围3mm切除肿瘤,并送病理,确定切除干净后在上方取游离结膜瓣修补缺损处,术后双眼包扎,每日换药,于术后7d拆除结膜缝线…     

儿童角膜结膜鳞状细胞癌1例

患儿周×　男　 10岁　右眼长肿物 1年。于 1年前发现右眼球内侧近角膜处长一灰白色斑块 ,不痛 ,无异物感、无畏光和流泪 ,不影响视力 ,故没有就诊。半年前发现肿物生长增快 ,已影响视物 ,故来本院就诊。就诊前未做过任何治疗。全身检查正常 ,耳前、颈部淋巴结无肿大。胸透心肺正常。肝胆脾胰Ｂ超检查正常声象图。视力右眼 4 5 ,左眼 5 0 ,右眼睑无肿胀 ,无内翻、外翻。角结膜内侧有一约 13ｍｍ×8ｍｍ的肿物 ,向前累及角膜达瞳孔中央 ,向后累及球结膜7ｍｍ。边界清楚 ,粉红色 ,表面凹凸不平 ,似菜花样 ,球结膜有一支粗大血管 ,呈树枝状进…     

眼睑鳞状细胞癌移植瘤模型建立及其VEGF的表达研究

目的建立眼睑鳞状细胞癌(SCC)移植瘤模型并研究其血管内皮生长因子(VEGF)的表达。方法BALB/c裸鼠40只,随机平均分为实验组和对照组,实验组裸鼠右上睑皮下接种Tca8113细胞建立眼睑SCC移植瘤,接种后第15、30 d,应用酶联免疫吸附检测(ELISA)法检测所有裸鼠血清中VEGF蛋白水平,并采用免疫组织化学法检测实验组移植瘤组织和对照组正常眼睑组织中VEGF的表达。结果实验组裸鼠右上睑均有SCC移植瘤形成,接种后第15、30 d,实验组裸鼠血清中VEGF质量浓度(188.6 pg/m l±79.93 pg/m l和200 pg/m l±75.28 pg/m l)均显著高于对照组(94.8 pg/m l±31.41 pg/m l和90.4 pg/m l±28.62 pg/m l)(P<0.01),两组间VEGF染色分数经统计学分析也存在显著差异(2.6±0.16和2.38±0.19,0.64±0.18和0.52±0.15)(P<0.01)。结论Tca8113细胞裸鼠眼睑皮下接种是建立眼睑SCC移植瘤的可行方法,靶向VEGF的基因治疗可望为眼睑SCC治疗提供新的途径。     

结膜鳞状细胞癌眼内浸润一例

患者女 ,4 7岁。因右眼球结膜鳞状细胞癌切除术后畏光、流泪、疼痛 ,伴视力下降 1个月 ,于 2 0 0 0年 10月 30日入院。患者于 2 0 0 0年 4月 2 0日发现右眼内眦处肿物 ,曾在外院行局部肿物和结膜切除术。术前眼部检查 :视力右眼 0 7,左眼 1 2 ;右眼内侧角膜缘 3点钟处球结膜呈灰白色隆起 ,肿物约 0 4ｃｍ× 0 3ｃｍ× 0 2ｃｍ ,局部可见扩张血管 ,角膜透明 ,余无异常。术后未进行任何治疗 ,病理诊断 :右眼结膜鳞状细胞癌。本次入院全身体检未见异常。眼部检查 :视力右眼 0 1,左眼 1 2。右眼球结膜混合性充血 (++) ,鼻侧球结膜增生肥厚 …     

眼睑色素性基底细胞癌的临床,病理及免疫组化

本文分析了我院１９８３－１９９０年收治的色素性基底细胞癌病人１９例，在收治的基底细胞癌６０例中占３１．６７％。年龄３０－３８岁，平均病程７．８４年。好发于下睑占６８％，光镜下该病例除含少量至中量色素外，细胞表现与基底细胞癌无差异。Ｓ－１００染色阴性，Ｋｅｒａｔｉｎ染色阳性，证明癌细胞来源于基底细胞。由于多表现带色素的结节，故临床易误诊为黑色素瘤。本文讨论该病的临床、病理特点及鉴别诊断。     

p16基因在喉鳞癌中的表达及其临床意义

目的 探讨p16基因在喉鳞癌中的表达及其临床意义.方法 应用原位杂交法检测49例喉鳞癌组织、19例声带息肉、12例癌旁增生组织及8例非典型增生组织中p16基因的表达情况.结果 喉鳞癌组织中p16基因表达呈阳性者5例,定位于胞浆,其余44例呈阴性表达.p16阳性表达例数在喉鳞癌组织、声带息肉、癌旁增生组织及非典型增生组织中分别为5,16,8,4例,喉鳞癌组织中p16mRNA的阳性表达显著低于声带息肉、癌旁增生组织及非典型增生组织(P＜0.01),但与喉鳞癌的l临床分期及患者性别无相关性(P＞0.05).结论 喉鳞癌组织中存在p16基因的低表达;p16基因可能参与调控了喉鳞癌的发生过程.     

Expression of p63 in conjunctival intraepithelial neoplasia and squamous cell carcinoma

Background p63 is a homologue of the tumour suppressor gene p53, which is expressed in human basal squamous epithelium. Some investigators maintain that p63 plays a role in the development of squamous epithelium and, despite its homology to p53, it is considered to act as an oncogene. This study investigated the expression of p63 in conjunctival intraepithelial neoplasia of different grades, and conjunctival squamous cell carcinoma and its correlation to the proliferation marker MIB-1. Material and methods Seventeen conjunctival specimens excised with the suspicion of either conjunctival intraepithelial neoplasia (CIN) or squamous cell carcinoma were diagnosed histologically as follows: 2 squamous cell carcinomas of the conjunctiva, 2 CIN grade I, 3 CIN grade II, 7 CIN grade III, 2 CIN with beginning invasion and 1 normal conjunctiva with no dysplasia. Sixteen microscopically-normal postmortem conjunctival specimens and normal conjunctiva, CIN and carcinoma specimens were stained immunohistochemically with antibodies against p63 and MIB-1. At least 500 cells per specimen were counted and the percentage of positively-stained cells of each antibody was calculated. Results A mean of 80% (57–89%) of the dysplastic cells from the CIN specimens stained positively with antibodies against p63, especially in the lower two-thirds of the epithelium, statistically significantly more compared with the normal specimens (9–55%, mean 36%, p<0.001). Nevertheless, we did not find a correlation between the percentage of p63-positive cells and the differentiation grade of the malignant specimens. MIB-1 positivity was shown by 0–1% of the cells in the normal postmortem controls, by 3–30% (mean 12%) of the cells in the basal and occasionally in the middle layer of the CIN specimens, and 16–61% (mean 23%) in the carcinoma specimens. Conclusion In conjunctival intraepithelial neoplasia and squamous cell carcinoma of the conjunctiva, p63 is preferentially expressed in the immature dysplastic epithelial cells. Its staining does not correlate with MIB-1-expression, and therefore does not appear to be linked to cell proliferation.     

Expression of the epidermal growth factor receptor in conjunctival squamous cell carcinoma

PURPOSE: To determine whether the tyrosine kinase epidermal growth factor receptor (EGFR) is expressed in squamous cell carcinoma of the conjunctiva. METHODS: A retrospective, observational case series of 5 patients with biopsy-proven conjunctival squamous cell carcinoma treated at one institution between January 1996 and April 2004. Medical records and tissue specimens from the 5 patients were reviewed. A control specimen of normal conjunctiva was also obtained from one of the 5 patients. Immunohistochemical analysis was performed using antibodies against EGFR. RESULTS: Immunohistochemical staining showed that EGFR expression was moderate to strong in all tissue specimens, both in the normal mucosa and in the in situ and invasive components of the conjunctival squamous cell carcinomas. CONCLUSIONS: An intense expression of EGFR occurs in squamous cell carcinoma of the conjunctiva. Further study is needed to determine whether an EGFR inhibitor that targets this expression would have potential therapeutic benefit in treating squamous cell carcinoma of the conjunctiva.     

Sclerokeratitis and invasive conjunctival squamous cell carcinoma

PURPOSE/METHODS: To describe one case of invasive squamous cell carcinoma of the conjunctiva in a 74-year-old woman, who two years previously had presented with a lesion which appeared to be an anterior nodular scleritis. RESULTS/CONCLUSIONS: An anterior nodular scleritis, which did not respond to therapy, preceded the development of a squamous cell carcinoma of the conjunctiva adjacent to it. In our patient, the conjunctival squamous cell carcinoma could have masqueraded as a scleritis, delaying the correct diagnosis and allowing orbital spread of the tumor. A diagnosis of neoplasia must be considered when a scleritis shows an atypical appearance or does not respond to the usual therapy.     

Massive corneal and conjunctival squamous cell carcinoma

A patient with massive, protuberant squamous cell carcinoma of conjunctiva invading the whole cornea, so as to hang from the surface, was referred with a visual acuity of hand motion near to face. A microscopically-controlled, frozen section guided excision, followed by double-freeze-thaw cryoapplication to the sclera and the edges of the conjunctival bed and lamello-lamellar sclerokeratoplasty, was performed. Three years later the patient's visual acuity was 20/60 with no evidence of recurrence of the lesion. Frozen section guided excision with adjuvant cryotherapy and lamellar sclerokeratoplasty is a viable therapy for massive squamous cell carcinoma of cornea and conjunctiva.     

P185基因在喉鳞癌中的表达及其临床意义

目的 探讨P185基因在喉鳞癌组织中的表达及其临床意义.方法 采用原位杂交方法检测49例喉鳞癌组织、19例声带息肉、12例癌旁增生组织及8例非典型增生组织中P185基因的表达情况.结果 喉鳞癌组织中44例P185基因呈阳性表达,定位于胞浆.在声带息肉、癌旁增生组织及非典型增生组织中P185基因的阳性表达例数分别为0,4,5例,经统计学分析,喉鳞癌组织中P185基因的表达显著高于声带息肉、癌旁增生组织及非典型增生组织(P均＜0.01),与喉鳞癌的临床分型分期,性别无相关性(P均＞0.05).结论 喉鳞癌组织中P185基因呈高表达,提示P185基因可能参与调控了喉鳞癌的发生过程,且与喉癌的临床分期分型无关.     

VEGF和TIMP-2蛋白在喉癌中的表达及其临床意义

目的 探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)和组织基质金属蛋白酶抑制剂-2(tissue inhibitor of matrix metalloproteinase-2,TIMP-2)在喉鳞状细胞癌组织中的表达及其临床意义.方法 采用Envision免疫组织化学法检测38例喉癌组织(肿瘤组)和19例喉癌旁正常黏膜组织(对照组)中VEGF和TIMP-2的表达.结果 肿瘤组中VEGF和TIMP-2的阳性表达率分别为94.74%和52.63%,均高于对照组的64.42%和21.05%(χ2=5.253,χ2=3.967,P＜0.05),两者表达呈正相关(rδ=0.558,P＜0.01);肿瘤组VEGF在声门型喉癌组织中的阳性表达率(88.89%)低于声门上型(100.00%)(χ2=25.735,P＜0.01),I Ⅱ期(88.89%)低于Ⅲ Ⅳ期(100.00%)(χ2=12.539,P＜0.05),病理高分化标本(85.71%)低于中分化(100.00%)(χ2=93.145,P＜0.01),而在有、无颈淋巴结转移组织之间的表达差异无统计学意义;TIMP-2在声门上型喉癌组织中的阳性表达率(50.00%)低于声门型(55.56%)(χ2=20.154,P＜0.01),高分化(51.43%)低于中分化(70.83%)(χ2=26.957,P＜0.01),有颈淋巴结转移的喉癌组织中(33.33%)低于无颈淋巴结转移者(61.54%)(χ2=6.748,P＜0.01),而在不同临床分期间的差异无统计学意义(χ2=0.062,P＞0.05).结论 VEGF和TIMP-2在喉鳞状细胞癌组织中呈高表达,而且在声门上型和声门型喉癌组织中的表达阳性率有差异;VEGF的表达可能与喉癌的临床分期、病理分化程度有关,而TIMP-2的表达可能与其病理分化程度及有无颈淋巴结转移有关.     

Intraocular extension of conjunctival squamous cell carcinoma

Conjunctival squamous carcinoma, which is regarded as a low-grade malignancy, usually originates at the limbus. Most cases remain superficial to the sclera. Intraocular invasion is rarely reported. We describe a woman misdiagnosed as conjunctivitis and pterygium before a clinical diagnosis of conjunctival squamous cell carcinoma. The diagnosis was made by histopathological examination of the biopsy specimen. Examination revealed an elevated mass on the nasal limbus extending intraocularly. White flaky mass occupied approximately 3/7 space of the anterior chamber. Exenteration was performed for control of local lesion. Histopathologic analysis confirmed that intraocular involvement occurs through the emissary vessels near the area of limbus. The case highlights the need for accurate diagnosis and prompt intervention. A brief review of the clinical and histopathologic features of conjunctival squamous cell carcinoma is also presented.