白河县204例0～3岁婴幼儿25-羟维生素D检测分析

目的:调查0~3岁婴幼儿血清25-羟维生素D(25-(OH)D)水平,为临床诊断和预防维生素D缺乏性佝偻病提供实验室依据。方法:2012年3月~2014年2月儿科门诊就诊的0~3岁婴幼儿204例,用酶联免疫吸附试验(ELISA)进行血清25-(OH)D浓度检测。结果:204例0~3岁婴幼儿中,血清25-(OH)D较为足够99例,占48.6%;相对缺乏79例,占38.7%;缺乏26例,占12.7%,前两者占总人数的87.3%。血清25-(OH)D以2~3岁组最高,0~1岁组最低,不同年龄组比较差异有统计学意义(P0.05),不同性别间25-(OH)D水平差异无统计学意义(P0.05)。结论:婴幼儿25-(OH)D缺乏率较高,其中以2~3岁幼儿25-(OH)D血清水平最低。     

那曲县0～3岁婴幼儿25-羟维生素D水平分析

目的了解那曲县0~3岁婴幼儿血清25-羟维生素D(25-OH-D)水平。方法选取2013年10月至2014年10月在西藏那曲县防保服务中心就诊的213例0~3岁婴幼儿,按照年龄分为0~1岁、>1~2岁、>2~3岁3个年龄组,采用酶联免疫法检测血清25-OH-D水平。结果婴幼儿血清25-OH-D平均浓度为(25.31±10.59)ng/mL,65例(占30.52%)儿童为缺乏,83例儿童为不足(占38.97%),65例(占30.52%)儿童为充足。各年龄组间血清25-OH-D水平有显著性差异,>1~2岁组血清25-OH-D水平最高,0~1岁组最低(F=5.895,P<0.01)。不同季节时期儿童血清25-OH-D水平有显著性差异,其中夏季儿童血清25-OH-D水平最高,冬季最低(F=10.722,P<0.05);冬季儿童维生素D缺乏比例最高(x^2=15.175,P<0.01);夏季儿童维生素D不足比例最低(x^2=11.433,P<0.05)。同一季节内,男女儿童血清25-OH-D水平、维生素D缺乏与不足比例差异均无统计学意义(均P>0.05)。结论那曲县0~3岁婴幼儿血25-OH-D水平低,维生素D缺乏、不足率较高。     

0~14岁儿童25-羟维生素D 水平调查与分析

目的 了解0~14岁儿童体内维生素D的营养状况, 为本地区儿童合理补充维生素D提供科学依据。方法 对广州中山大学附属第三医院儿童保健门诊进行常规体检的1 000例0~14岁的儿童采用酶联免疫法检测血清25-羟维生素D[25-hydroxy vitamin D, 25-(OH)D]水平。结果 25-(OH)D缺乏及不足者634例(63.4%);25-( OH) D水平充足者366例(36.6%)。0~1和1~2岁组儿童血清25-(OH)D水平最高, 2岁后儿童随着年龄增长逐渐下降(P＜0.01)。0~7岁男、女童血清 25-(OH)D水平差异无统计学意义(P＞0.05), 但在7~14岁组儿童男、女比较差异有统计学意义(P＜0.01)。血清25-(OH)D水平夏＞秋＞春＞冬, 冬季维生素D缺乏或不足的检出率为70.29%, 高于其他季节(P＜0.05)。结论 0~14岁儿童 25-(OH)D平均水平低下, 普遍存在维生素D缺乏或不足, 特别是在冬季和年长儿童。     

金华市部分0～6岁儿童25-羟维生素D水平分析

【目的】了解金华金华婴幼儿25-羟维生素D(25-OHD)水平,关注维生素D缺乏性佝偻病。【方法】对来院儿科门诊检查的0~6岁4 836例儿童,采用酶联免疫法进行25-OHD检测,对结果进行统计分析。【结果】4 836例儿童中25-OHD水平较为足够(合适水平)的为1 564例,占32.3%;相对缺乏(临界水平)的为2 520例,占52.1%;缺乏的为752例,占15.6%。后两者占总人数的68.0%,以0~2岁婴幼儿为主,不同年龄组25-OHD均值差异有统计学意义(F=26.45,P0.05)。性别间25-OHD水平差异无统计学意义(t=1.340,P0.05);区域间25-OHD水平差异有统计学意义(t=6.417,P0.05)。【结论】应加强婴幼儿的户外活动并系统管理。     

0～3岁婴幼儿维生素D水平检测结果及缺乏的风险因素分析

目的了解0~3岁婴幼儿维生素D营养状况,分析相关影响因素,为婴幼儿维生素D的合理补充提供依据。方法选取2015年6月-2017年6月在该院儿童保健门诊体检的1 300例0~3岁婴幼儿为研究对象,均行血清25-羟基维生素D~([25-(OH)-D])检测。结果 1 300例儿童中,131例出现维生素D缺乏(10.08%),246例出现维生素D不足(18.92%)。男童与女童血清25-(OH)-D水平及维生素缺乏率、不足率比较差异无统计学意义(t=1.749,χ~2=1.648、0.301,P0.05),≤1岁、1~2岁、2~3岁维生素D缺乏率分别为17.90%、6.90%、10.62%(χ~2=22.746,P0.01),且在血清25-(OH)-D水平上比较差异有统计学意义(F=15.264,P0.01);维生素D缺乏率冬季最高(14.55%),夏季最低(6.97%),不同季节比较差异有统计学意义(χ~2=8.541,P0.01);纯母乳喂养儿童维生素D缺乏率高于部分母乳喂养、配方奶喂养(13.00%vs.9.38%、5.44%),差异有统计学意义(χ~2=14.371,P0.01);户外活动时间1 h儿童维生素D缺乏率高于户外活动时间1~2 h儿童(14.15%vs.8.15%),差异有统计学意义(χ~2=11.233,P0.01)。Logistic回归分析结果显示,年龄≤1岁、户外活动时间1 h、纯母乳喂养是维生素D缺乏的独立危险因素。结论 0~3岁婴幼儿维生素D缺乏情况与年龄、户外活动时间、喂养情况有关。     

扬州地区婴幼儿25-羟维生素D水平调查分析

目的 了解扬州市婴幼儿25-羟维生素D水平及现状,为科学合理指导婴幼儿维生素D缺乏提供理论依据。方法 对2012年8-12月来本院儿童保健门诊进行常规检查的986例婴幼儿进行静脉末梢血血清25-羟维生素D水平测定。结果 986例婴幼儿血清25-羟维生素总体水平为(87.33±35.77)nmol/L,性别差异无统计学意义。25-羟维生素D水平缺乏、不足、适宜水平的检出率为分别为11.4%、32.3%、56.4%。季节变化对婴儿的维生素D水平的影响不明显,而1~2 h/d户外活动及常规补充维生素D可有效预防维生素D缺乏。结论 扬州地区婴幼儿维生素D水平普遍较低,应对家长进行有关科学预防维生素D缺乏知识的宣传教育,对婴幼儿参加户外活动及维生素D服用情况进行系统管理。     

南京市0～3岁婴幼儿25羟维生素D缺乏与贫血关系的研究

目的 分析南京市0～3岁婴幼儿25羟维生素D[25-(OH)D]水平,并研究其与贫血的相关性,为儿童贫血防治提供理论依据。方法 选取江苏省人民医院妇幼分院儿童保健门诊2017年8月-2019年10月共1 143例常规体检的0～3岁婴幼儿,对其末梢血25-(OH)D、血红蛋白(Hb)结果进行统计分析;并分析各年龄段25-(OH)D水平与Hb的相关性。结果 1 143例0～3岁婴幼儿25-(OH)D平均水平为(79.03±25.71)nmol/L,其中缺乏者113例(9.88%),不足者451例(39.46%),充足者579例(50.66%);不同年龄段25-(OH)D水平差异有统计学意义(F＝25.26,P＜0.01),以2～3岁组水平最低;贫血者122例(10.67%),非贫血者1 021例(89.33%),25-(OH)D缺乏组贫血检出率明显高于充足组,差异有统计学意义(F＝8.261,P=0.004);Pearson相关性分析发现25-(OH)D水平与Hb在0～、0.5～、1～2岁组均存在正相关性(r=0.288,0.274,0.123, P<0.05),2～3岁组未发现相关性(r=0.080,P=0.264)。结论 南京市0～3岁婴幼儿25-(OH)D总体缺乏和不足率较高;0～2岁年龄段婴幼儿25-(OH)D水平与Hb存在正相关性,提示25-(OH)D缺乏可能影响Hb的生成,与婴幼儿贫血的发生相关。     

新会地区婴幼儿维生素D的营养状况研究

目的了解江门市新会地区婴幼儿维生素D的营养状况,为指导维生素D的合理应用提供依据。方法随机选取2013年4月-2014年3月在广东省江门市新会区妇女儿童医院儿童保健门诊健康体检的0~3岁儿童300例,采用高效液相色谱-串联质谱法测定血清25羟基维生素D水平。结果新会地区婴幼儿血清25-(OH)D平均水平为(29.13±8.12)ng/ml,血清25-(OH)D水平随年龄增加而下降,不同年龄段和不同季节血清25-(OH)D比较差异有统计学意义(P0.05),维生素D的缺乏率为2.3%、不足率为8.0%,不同年龄段维生素D不足率比较差异有统计学意义(P0.05)。结论新会地区婴幼儿维生素D营养状况较好,应重点加强幼儿维生素D的补充。通过定期检测血清25-(OH)D了解体内维生素D水平,科学指导维生素D的补充,消除家长对过量中毒的担忧,从而增加家长的依从性。     

湖州市3 724例0~6岁门诊体检儿童维生素D营养状况

目的 了解0～6岁门诊体检儿童的维生素D营养状况,为科学合理地补充维生素D制剂提供理论依据.方法 选取2013年1月至2014年3月在湖州市妇幼保健院儿童保健门诊进行常规体检的3 724例0～6岁儿童,采用电化学发光法检测血清25-(OH)D的水平,比较不同年龄段儿童及不同季节血清25-(OH)D水平及维生素D不足与缺乏情况.结果 0～6岁门诊体检儿童的血清25-(OH)D平均水平为(37.80±11.67) ng/ml,维生素D不足与缺乏的比例为7.33％,男女童之间差异均无统计学意义(t=-1.57,P=0.117;x2=0.37,P=0.543).＞3且≤6岁儿童血清25-(OH)D水平显著低于≤1岁、＞1且≤2岁和＞2且≤3岁儿童[(27.36±8.90) ng/ml比(38.64±12.10) ng/ml,t=18.60,P=0.000;比(41.63±10.31) ng/ml,t=26.07,P=0.000;比(36.85±10.01) ng/ml,t=16.42,P=0.000],维生素D不足与缺乏比例显著高于其他3个年龄段儿童(104/466比122/1 487,x2=69.06,P=0.000;比18/1 106,x2=196.06,P=0.000;比29/665,x2=85.14,P=0.000).冬季儿童25-(OH)D水平显著低于春、夏、秋季[(35.16±11.30) ng/ml比(40.03±12.57) ng/ml,t=9.15,P=0.000;比(36.86±10.60) ng/ml,t=3.34,P =0.001;比(39.99±11.36) ng/ml,t=9.65,P=0.000],维生素D不足与缺乏的比例显著高于其他3个季节(127/1 189比56/849,x2=10.11,P=0.001;比54/787,x2=8.30,P=0.004;比36/899,x2 =31.71,P=0.000).结论 本院0～6岁门诊体检儿童的总体维生素D营养状况良好,冬季和＞3且≤6岁儿童维生素D水平较低、不足与缺乏的比例较高,因此应重视冬季和3岁以上儿童的维生素D补充,适当增加儿童户外活动,同时加强科学预防维生素D缺乏的宣传教育.     

67例儿童血清维生素A、D、E水平研究

目的了解婴幼儿血清维生素A、D、E水平状况,为临床诊治工作提供参考。方法以2013年1-3月在首都儿科研究所附属儿童医院保健门诊67名要求健康体检儿童作为研究对象,采用高效液相色谱检测血清维生素A、25羟维生素D[25(OH)D]、维生素E水平,收集其基本情况、喂养方式、体格等方面信息。结果 67名儿童中,男40例,女27例。维生素A水平为(0.23±0.07)mg/L,缺乏率34.3%,不同月龄段血清水平差异有统计学意义(F=5.866,P=0.001),随月龄增加血清维生素A缺乏率逐渐减少,0~4个月组最低[(0.17±0.04)mg/L],缺乏率高达68.8%;不同喂养方式血清维生素A水平差异有统计学意义(F=2.838,P=0.045),纯母乳喂养组血清水平偏低[(0.18±0.03)mg/L],缺乏率为60.0%。血清25(OH)D水平为(102.26±90.54)nmol/L,缺乏率为13.3%;不同喂养方式水平差异有统计学意义(F=2.951,P=0.038),纯母乳喂养婴儿仍偏低[(96.93±20.42)nmol/L],缺乏率较高为33.3%。血清维生素E水平为(10.09±2.76)mg/L,不足率仅为13.4%,未发现缺乏儿童,维生素E营养状况良好。结论婴幼儿维生素A、D营养状况不容乐观,应针对高危人群及时施加干预以预防维生素缺乏症的发生。     

Total,Bioavailable, and Free 25-Hydroxyvitamin D Equally Associate with Adiposity Markers and Metabolic Traits in Mexican Adults

Epidemiological studies suggest a relationship between total 25-hydroxyvitamin D [25(OH)D], adiposity, and metabolic traits. The bioavailability of 25(OH)D is regulated by the albumin, vitamin D binding protein (VDBP), and variants of the GC gene. Therefore, it is not clear if bioavailable or free 25(OH)D offer additional benefits compared to total 25(OH)D when estimating the magnitude of these associations. Our aim was to evaluate the association between 25(OH)D (total, free and bioavailable) with adiposity and metabolic traits. This was a cross-sectional study of 1904 subjects from the Health Workers Cohort Study from Mexico. Free and bioavailable 25(OH)D were calculated based on VDBP and albumin determinations, using a formula adjusted for the GC gene diplotypes. Adiposity and metabolic traits were measured with standardized procedures. Free and bioavailable 25(OH)D levels correlated with total 25(OH)D, r = 0.71 and 0.70, respectively (p < 0.001). Total, bioavailable and free 25(OH)D levels were negatively associated with the adiposity marker (visceral adiposity index) and metabolic traits (metabolic syndrome, type 2 diabetes, triglycerides, triglycerides/HDL-c ratio, and triglycerides/glucose index) in multivariate regression models (ORs = 0.73 to 0.96). Our findings suggest that free and bioavailable 25(OH)D do not offer additional advantages over total 25(OH)D regarding its association with adiposity and several metabolic traits in Mexican adults.     

VITAMIN D METABOLISM IN PATIENTS WITH SMALL-BOWEL RESECTION

Malabsorption of vitamin D after small-bowel resection occurs and apparently is due not only to interruption of the enterohepatic circulatory system of bile acids, but also to a primary failure of the small intestine to absorb vitamin D.     

Older Mayan residents of the western highlands of Guatemala lack sufficient levels of vitamin D

Vitamin D (VitD) levels in older Mayans are currently unknown. Geographic factors, for example, residences in areas receiving ample sunlight at high altitudes and latitudes near the equator, would favor optimum VitD levels, whereas demographic factors, for example, darker skin pigmentation, clothing practices, and older age, would favor low 25-hydroxy-vitamin D, or 25(OH)D, levels. Conjecturing that demographic factors affecting VitD status might outweigh geographic factors in this population, we hypothesized that older Mayans have suboptimal values of 25(OH)D. We also hypothesized that older Mayans in rural areas would have higher VitD levels than would their urban counterparts. Blood samples were collected from 108 healthy older Mayans (mean age, 69 years) from urban (n = 84, 50% male) and rural settings (n = 24, 50% male) during the summer of 2008 in the highlands of Quetzaltenango, Guatemala. We assessed 25(OH)D concentrations by radioimmunoassay in a US-based laboratory. Mean (SD) serum 25(OH)D values were 53.3 (15.0) nmol/L, and lower 25(OH)D values were associated with increasing age (r = −0.58, P = .004). Of all subjects, 3.7% (n = 4) maintained an optimal status of 25(OH)D (>80 nmol/L), 50% (n = 54) had values between 50 and 80 nmol/L, and 46.3% (n = 50) had levels less than 50 nmol/L. Urban subjects had nonsignificantly higher 25(OH)D values (55.0 ± 15.3 nmol/L) than did rural subjects (47.4 ± 12.4 nmol/L, P = .228). Men had significantly higher values (58.2 ± 16.5 nmol/L) than did women (48.4 ± 11.6 nmol/L, P = .001). We conclude that despite residing in an optimal geographic location to receive adequate sunlight exposure, most older Guatemalan Mayans in Quetzaltenango have suboptimal levels of VitD.     

Relative concentrations of 25-hydroxyvitamin D2/D3 and 1,25-dihydroxyvitamin D2/D3 in maternal plasma at delivery

Quantitation of circulating 25-OH-D₂, 25-OH-D₃, 1,25-(OH)₂-D₂ and 1,25-(OH)₂-D₃ was performed on the plasma from 10 women collected at delivery following full term pregnancies. These data indicate that approximately 50% of the circulating 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D in these women are in the vitamin D₂ form. Further, they demonstrate that vitamin D₂ contributes significantly to the total vitamin D status of these individuals and cannot be considered of trivial importance. It is apparent from this study that assay techniques which specifically quantitate vitamin D₂, as well as D₃ must be employed when measuring the total vitamin D status of individuals who are consuming significant quantities of dietary vitamin D₂.     

血清1,25(OH)2D和25(OH)D的测定及正常值的建立

目的　建立 1,2 5 (OH) 2 D和 2 5 (OH)D的长沙居民正常值。　方法　选择长沙居民 13 7例 ,年龄 3 0～ 75岁 ,使用RIA方法测定血清上述指标正常值。　结果　两项指标皆随年龄下降 ,对于 2 5 (OH )D ,<40岁 ,40～ 60岁以及>60岁 ,三年龄组分别为 (2 5 .5± 3 .6) ,(2 1.3± 3 .3 )以及 (18.9± 3 .3 )ng/ml ,1,2 5 (OH) 2 D三年龄组分别为 (3 2 .2± 3 .1) ,(2 7.4± 3 .4)以及 (2 5 .7± 3 .7) pg/ml组间有显著差异。　 结论　 1,2 5 (OH) 2 D和 2 5 (OH)D血清正常值随增龄而下降 ,必须按年龄段分别确定正常值 .     

Relationship between Serum 25OH-Vitamin D2 Level and Vitamin D Status of Children Aged 3–5 Years in China

Background: Vitamin D deficiency is prevalent globally and there is lack of evidence as to how 25(OH)D2 contributes to vitamin D status. The aim of this study was to describe vitamin D status and to assess the role of vitamin D2, a dietary vitamin D source, against the vitamin D status of children aged 3–5 years in China. Methods: Data were extracted from the Chinese National Nutrition and Health Surveillance (CNNHS) in 2013. The concentration of serum 25(OH)D2 and 25(OH)D3 was measured by using LC-MS/MS. Results: A total of 1435 subjects were enrolled and serum 25(OH)D were analyzed. The prevalence of total serum 25(OH)D < 30 nmol/L was 8.9%. Serum 25(OH)D2 was detected in 10.9% of the studied children. After adjusting for confounding factors, total 25(OH)D concentration was 8.48 nmol/L lower and odds ratio of vitamin D deficiency was 4.20 times (OR (95%CI): 4.20 (1.64, 10.77)) in children without 25(OH)D2 than those with 25(OH)D2 detected. Conclusions: Vitamin D deficiency was common among children aged 3–5 years in China. Vitamin D2 may play a role in preventing vitamin D deficiency in Chinese children aged 3–5 years.     

Association between Vitamin D Status and Premenstrual Symptoms

Background

Premenstrual symptoms are experienced by up to 95% of women, and few treatments are available. Previous studies suggest that 25-hydroxyvitamin D (25(OH)D) may be associated with the severity of premenstrual symptoms, but the findings have been inconclusive.

824例婴幼儿血清25-羟基维生素D检测结果分析

目的:了解广州市区0～6岁婴幼儿的维生素D营养状况.方法:收集在广东省妇幼保健院儿保科门诊进行常规保健的824例0～6岁婴幼儿血清,采用酶联免疫吸附试验(ELISA)检测血清25-羟基维生素D[25-(OH) D].结果:824例0～6岁要幼血清25-(OH)D的平均水平为(69.52±57.09) nmol/L,其中充足组213例占25.85％,有73.66％的婴幼儿25-(OH)D处于较低水平(51.33％不足、19.42％缺乏、2.91％严重缺乏).0～6岁婴幼儿血清25-(OH)D水平在不同性别间差异无统计学意义(P＞0.05);3～6岁组婴幼儿血清25-(OH)D水平明显低于＜1岁组,差异有统计学意义(P＜0.05).结论:广州市区0～6岁要幼儿血清25-(OH)D水平普遍偏低,应重视婴幼儿血清25-(OH)D的检测.     

120例0～6岁儿童血清25-羟基维生素D水平的调查

目的:了解中山小榄地区儿童维生素D的营养状况.方法:选择2013年在广东医学院附属陈星海医院儿童保健科和儿科就诊的0～6岁120例儿童为研究对象,采用高效液相质谱法-串联质谱法进行血清25-(OH)D水平检测,并对结果进行统计学分析.结果:120例儿童25-(OH)D水平为(25.7±7.1)ng/ml,婴儿组25-(OH)D水平(31.3±7.2) ng/ml高于幼儿组的(25.7±6.2)ng/ml,幼儿组高于学龄前组的(22.8±7.1)ng/ml;男性儿童25-(OH)D水平(24.2±7.3) ng/ml低于女性儿童的(28.2±6.1)ng/ml,差异均有统计学意义(P＜0.05).儿童25-(OH)D缺乏率为71.7％,幼儿组与学龄前组血清25-(OH)D缺乏率差异无统计学意义(P＞0.05),但高于婴儿组缺乏率(P＜0.05);男性儿童VitD缺乏率73.7％与女性儿童68.2％差异无统计学意义(P＞0.05).结论:中山小榄地区儿童25-(OH)D缺乏率较高,男性和女性缺乏率无差异,25-(OH)D水平随着儿童年龄增大而下降.