宫颈癌盆腔淋巴结切除临床处理的循证评估

目的分析临床宫颈癌手术淋巴结切除处理方法,对其利弊进行评估。方法回顾2008。2011年3年间105例宫颈癌Ⅲ型子宫切除同时盆腔淋巴切除患者的淋巴病理检查结果,分析盆腔淋巴结切除的临床利弊和病理结果的可靠性。结果（1）临床上未能了解淋巴结内肿瘤细胞的生物学活性,难以区分细胞是侵入生长或被捕获杀伤凋亡。（2）部分病例切除淋巴结未达到95％切除,每一病例病理报告未达到95％的淋巴切片,阴性结果难以具有统计学意义。（3）术中无法确定1-2mm的淋巴完全切除。（4）文献报道术前CT检查发现淋巴结转移敏感度为66．7％。特异度为100％,漏诊率为33．3％,误诊率为0。结合PET探测结果更加准确。结论（1）判断淋巴结中的肿瘤细胞是免疫反应还是浸润转移,是盆腔淋巴结切除术必要性的关键之一。（2）“淋巴结病理阴性”结论难有统计学的意义,“淋巴转移阴性结果”只有参考作用。（3）手术可能有阳性淋巴遗漏。（4）CT、PET-CT的影像学阳性诊断指导下淋巴结切除的临床意义会更确定。     

子宫内膜癌组织中成纤维细胞激活蛋白的表达及其与微血管密度的关系

目的 探讨成纤维细胞激活蛋白(FAP)在子宫内膜癌间质中的表达及其与微血管密度(MVD)的关系.方法 收集82例子宫内膜癌组织及20例经病理证实的子宫内膜息肉,采用免疫组织化学染色法检测FAP和微血管特异性标记物CD34的表达.分析FAP表达与MVD的关系.结果 在子宫内膜癌组织中,FAP表达于间质成纤维细胞,表达阳性率为89.02%(73例),而子宫内膜癌细胞和子宫内膜息肉中无表达.FAP表达和MVD分别与子宫内膜癌的分化程度、侵袭深度及淋巴结转移有关(均P＜0.01).随着FAP表达水平的增高,MVD也随之增加.结论 FAP的表达与子宫内膜癌的分化程度、侵袭深度及淋巴结转移有关.FAP可能通过促进肿瘤的微血管生成,加快肿瘤的生长、侵袭和转移.     

Evaluation of the morphology of submucosal tumor invasion and its volume in early gastric cancer

BACKGROUND: The aim of the present study was to evaluate the clinical importance of the morphology of submucosal tumor invasion and its volume in early gastric cancer. MATERIALS AND METHODS: The subjects were 92 patients with a single lesion of early gastric cancer, who underwent gastrectomy with dissection of lymph nodes, and in whom the lesion was histologically-diagnosed as early gastric cancer with submucosal invasion. The volume of lesions (Vsm, S: < 50 mm3, L: > = 50 mm3) was determined by reconstructing submucosal lesions by the surface rendering method using pathological tissue sections (mean, 3.6 sections/lesion). The relationships between the volume of lesions and lymph node metastasis and between conventional clinicopathological parameters and lymph node metastasis were evaluated. RESULTS: Lymph node metastasis was observed in 18 patients (20%). The depth of submucosal invasion was 1.7 +/- 1.4 mm (mean +/- S.D.; range, 0.2-7.5 mm). sm1 (depth of submucosal tumor invasion < 0.5 mm) was observed in 11 patients (12%), and sm2 (depth of submucosal tumor invasion > = 0.5 mm) in 81 patients (88%). The mean Vsm was 104.1 +/- 215.4 mm3 (0.4-1,730.5 mm3). Fifty-seven patients (62.0%) were in group S, and 35 patients (38.0%) in group L. There was a significant difference between lymph node metastasis and Vsm (S vs. L) alone (p = 0.006). Logistic regression analysis also demonstrated that Vsm alone was correlated with lymph node metastasis (p = 0.005; odds ratio, 1.27; 95% confidence interval, 0.074-0.401). CONCLUSION: The volume of submucosal lesions in early gastric cancer is important for evaluating lymph node metastasis.     

A case for gastrointestinal stromal tumor (GIST) with reference to its ultrastructure and 'gain-of-function' mutation

A case for primary gastrointestinal stromal tumor (GIST) is described with reference to its ultrastructural characteristics and mutation within the exon 11 of c-kit gene. A forty-seven years old woman complaining of dysphasia was examined by endoscopy, which depicted a submucosal tumor (70 mm in diameter) with ulcerations at the fundus of the stomach. Histopathologically, the tumor cells had large nuclei and eosinophilic cytoplasm and were frequently during mitosis phase. The tumor cells were immunopositive for KIT, CD 34 and vimentin, suggesting their fibroblast-like characteristics. In contrast, desmin and S-100, a smooth muscle and an enteroglial marker, were not immunopositive within the cells. At least 30 % of the tumor cells possessed MIB-I and 20 % of them possessed p53, which are compatible with fast development of the tumor. By electron microscopy, the tumor cells possessed large oval nuclei, abundant mitochondria, caveolae and smooth endoplasmic reticulums, while no gap junctions were seen on the cells: The tumor cells thus possessed interstitial cells-like characteristics at least in part. DNA mutation search for the tumor cells however realized no gain-of-function mutation within the exon 11 of the c-kit gene, suggesting existence of other mechanism for neoplasmic growth of the tumor cells classified as gastrointestinal stromal tumors.     

卵巢交界性子宫内膜样肿瘤4例临床病理观察

目的 深入了解卵巢交界性子宫内膜样肿瘤(EBT)的临床病理特点、鉴别诊断、治疗和预后.方法 对清远市人民医院病理科2012年1月至2019年9月诊断的4例卵巢EBT的临床资料、组织学形态、免疫组化特点及预后进行分析总结,并复习相关文献.结果 4例因不规则阴道出血、下腹胀痛或发现盆腔占位入院,均行子宫及双附件切除术,其中...     

Six-month toxicity study of butylated hydroxyanisole in beagle dogs

Groups of three or four male and female beagle dogs were maintained for 6 months on diets containing BHA at concentrations of 0 (control group), 0.25, 0.5 and 1.0% (w/w). The average daily intake of BHA was about 60 mg/kg body weight in the 0.25% group, 110 mg/kg in the 0.5% group and 220 mg/kg in the 1.0% group. All animals survived the entire experimental period without showing signs of toxicity other than a dose-related retardation of growth. Serum biochemical examinations conducted at 1, 3 and 6 months revealed a slight decrease in albumin concentration and an elevation of alkaline phosphatase and leucine aminopeptidase activity in the higher dose groups. Histopathological and histometrical examination showed no evidence of mucosal alteration in the stomach, oesophagus or duodenum attributable to the administration of BHA. The mitotic index in the squamous epithelium of the distal oesophagus was comparable in the control and BHA-treated groups. Liver weights were increased in BHA-treated groups, but there were no related histopathological changes. From these data it is concluded that feeding of BHA-supplemented diets at palatable concentrations for 6 months has no pathological effects on the stomach, oesophagus, duodenum or liver of beagle dogs.     

早期胃癌临床分析68例

目的探讨早期胃癌(EGC)的诊断、治疗方法及病理特点。方法回顾性分析1980年9月至2004年12月在周口市中心医院接受手术治疗EGC患者的临床资料。结果本组患者共68例,男性50例,女性18例;年龄32～74岁,平均53.4岁。肿瘤部位:贲门部1例,胃体4例,胃角16例,胃窦45例,双原发癌2例(胃窦+胃体,胃角+胃体各1例)。本组患者症状无特异性,以上腹疼痛为首诊症状35例,占51.5%,合并嗳气、反酸60例,占88.2%;合并恶心、呕吐、食欲不振、乏力、消瘦10例,占14.7%;腹胀、腹泻14例,吞咽困难2例,黑便3例,体检发现1例。上消化道X线造影、B超的EGC检出率分别为14.7%和26.5%。全部行胃镜检查,凹陷型62例(91.2%),隆起型8例,病理确诊率92.6%。本组全部接受胃癌根治术,其中D241例(60.3%),D127例(39.7%),远端胃癌根治术63例,近端胃癌根治术1例,根治性全胃切除术4例。手术病死率为0%,无1例吻合口瘘。肿瘤最大径6.5cm,平均2.34cm。术后病理分型:低分化腺癌4例,中分化腺癌16例,高分化腺癌2例,黏液细胞癌23例,印戒细胞癌2例,溃疡癌变17例。黏膜内癌67例,黏膜下癌1例,一点癌1例,息肉恶变及萎缩性胃炎癌变各1例。未见淋巴结转移。11例患者术后行化疗,化疗方案主要采用CF+5-FT-207。获得随诊63例,随诊率92.6%,随诊时间13个月～17年。术后病程超过5年者24例,死亡3例(1例肝、胰腺转移,2例肝转移),5例失访。术后5年生存率为91.9%,病死率4.4%。结论根治性手术治疗是EGC取得良好疗效的最佳途径。胃癌根治术是治疗EGC的标准术式。胃镜病理活检是EGC诊断的金标准。     

内质网蛋白29在食管癌中的表达及其临床意义

目的:探讨食管癌组织中ERP29的表达情况及其与食管癌临床病理特征以及预后等之间的关系。方法:食管癌切除手术标本60例,通过免疫组化检测ERP29在食管癌组织及癌旁食管正常组织中的表达情况。结果:ERP29在食管癌旁组织中的阳性表达率为85%,而在食管癌组织中的阳性表达率为43.3%,两者相比具有统计学差异(χ2=22.652,P<0.001)。食管癌组织ERP29的表达与患者年龄、性别、肿瘤部位、癌组织学类型及局部浸润深度等无明显相关性,但与肿瘤TNM分期及淋巴结转移呈负相关。患者食管癌组织ERP29表达阳性组1、2及3年生存率分别为92.3%、80.8%、65.4%,高于食管癌组织ERP29表达阴性组79.4%、55.9%、35.3%。根据Kaplan-Meier生存分析和log-rank检验,ERP29表达阳性与阴性患者相比,有统计学差异。结论:食管癌组织ERP29的表达与食管癌旁组织相比明显下降,并且食管癌组织ERP29的表达与肿瘤分期、淋巴结转移呈负相关,提示ERP29是一个潜在抑癌基因。ERP29表达与食管癌的预后相关,可作为预测食管癌预后的一个潜在指标。     

转移抑制子KISS1和nm23在结肠癌中的表达以及与转移的关系

目的观察KISS1和nm23在结肠癌及淋巴结转移组织中的表达,并探讨他们在肿瘤发生和转移中的作用。方法采用组织芯片和免疫组化方法检测KISS1和nm23在正常黏膜(n=126)、原发癌(n=126)和淋巴结转移癌(n=62)中的表达,比较它们在原发灶和正常组织、转移灶与原发灶中的表达差异。结果KISS1的表达随肿瘤的发生和进展逐步下降。KISS1在正常黏膜、原发癌和淋巴结转移组织中的表达率分别为:95.2%、73.8%和41.9%;而nm23在相应组织中的表达为91.2%、82.5%和56.5%。正常黏膜组织中KISS1的表达率显著高于原发癌中的表达,而转移灶中的表达率又显著低于原发灶中的表达。结论肿瘤转移是多基因参与、多阶段、多步骤的结果,转移抑制子如KISS1和nm23的表达在结肠癌发展和转移中起重要作用。     

胃黏膜下隆起性病变的内镜超声诊断与治疗

目的探讨内镜超声对胃黏膜下隆起性病变的诊断与治疗价值。方法应用内镜超声对40例胃黏膜下隆起性病变患者进行检查及引导治疗。结果经内镜超声检查诊断为平滑肌瘤18例（45．0％），胃间质瘤2例（5．0％），脂肪瘤2例（5．0％），胃底静脉瘤1例（2．5％），异位胰腺2例（5.0％），胃壁外压迫13例（32．5％），未见异常2例（5.0％）。胃壁外压迫性病变主要为脾脏（8／13，61．5％），其次是肝左叶、胆囊、胰腺囊肿、胰腺囊腺癌等。3例平滑肌瘤、2例脂肪瘤及2例异住胰腺经内镜下切除；3例平滑肌瘤、2例良性胃间质瘤、1例胰腺囊肿及1例胰腺囊腺癌经手术治疗。病理与内镜超声诊断符合率为100％。结论内镜超声诊断胃黏膜下隆起性病变较为准确，能对病变的性质及起源做出诊断，并对治疗方法的选择提供参考。     

内镜下黏膜切除术及剥离术在早期消化道肿瘤中的应用

目的 探讨内镜下黏膜切除术(EMR)和内镜黏膜下剥离术(ESD)治疗消化道黏膜层病变包括癌的疗效和安全性。方法 对行EMR和ESD治疗的215例患者260处消化道病变做回顾性分析。结果 食管病灶4处,胃70处,大肠186处,其中EMR 185例,ESD 30例。ESD病变长径、手术时间均明高于EMR;EMR术后2例迟发性出血,无穿孔,ESD术后1例迟发性出血,术中穿孔2例,皆内镜下钛夹夹闭治疗后治愈。术后病理确诊早期癌变7处,高级别上皮内瘤变28处,腺瘤性息肉伴低级别上皮内瘤变161处,间质瘤6处,炎性息肉及其他58处。术后3个月和6个月行内镜随访,无病变残留和复发。结论 EMR和ESD技术治疗消化道黏膜层病变是安全有效的,可以提供完整的病理诊断学资料。EMR和ESD可用于治疗消化道早期癌变及癌前病变。     

ECL Cell Histamine Mobilization Studied byGastric Submucosal Microdialysis in Awake Rats:Methodological Considerations

Abstract: The ECL cells are endocrine/paracrine cells in the acid‐producing part of the stomach. They secrete histamine in response to circulating gastrin. Gastric submucosal microdialysis has been used to study ECL‐cell histamine mobilization in awake rats. In the present study we assess the usefulness and limitations of the technique. Microdialysis probes were implanted in the gastric submucosa. Histological analysis of the stomach wall around the probe revealed a moderate, local inflammatory reaction 1–2 days after implantation; the inflammation persisted for at least 10 days. Experiments were conducted 3 days after the implantation. The “true” submucosal histamine concentration was determined by perfusing at different rates (the zero flow method) or with different concentrations of histamine at a constant rate (the no‐net‐flux method): in fasted rats it was calculated to be 87±5 (means±S.E.M.) nmol/l and 76±9 nmol/l, respectively. The corresponding histamine concentrations in fed rats were 93±5 and 102±8 nmol/l, respectively. With a perfusion rate of 74 μl/hr the recovery of submucosal histamine was 49%, at 34 μl/hr the recovery increased to 83%. At a perfusion rate below 20 μl/hr the microdialysate histamine concentration was close to the actual concentration in the submucosa. The ECL‐cell histamine mobilization was independent of the concentrations of Ca²⁺ in the perfusion medium (0–3.4 mmol/l Ca²⁺). In one experiment, histamine mobilization in response to gastrin (10 nmol/kg/hr subcutaneously) was monitored in rats pretreated with prednisolone (60 mg/kg) or indomethacin (15 mg/kg). The two antiinflammatory agents failed to affect the concentration of histamine in the microdialysate either before or during the gastrin challenge, which was in accord with the observation that the inflammatory reaction was modest and that inflammatory cells were relatively few around the probe and in the wall of the probe. In another experiment, rats were given aminoguanidine (10 mg/kg) or metoprine (10 mg/kg) 4 hr before the start of gastrin infusion (5 nmol/kg/hr intravenously). Metoprine (inhibitor of histamine N‐methyl transferase) did not affect the microdialysate histamine concentration, while aminoguanidine (inhibitor of diamine oxidase) raised both basal and gastrin‐stimulated histamine concentrations. We conclude that microdialysis can be used to monitor changes in the concentration of histamine in the submucosa of the stomach, and that the inflammatory reaction to the probe is moderate and does not affect the submucosal histamine mobilization.     

Gastric secretion and ulceration induced in the rat by an extract from scorpion (B. quinquestriatus) telsons

A extract from scorpion telsons evoked gastric secretion rich in pepsin, with increased titratable acidity, and a decrease in total acid output. In rats treated with hydergine plus scorpion telson extract, the gastric juice was richer in acid and pepsin than that evoked by either scorpion telson extract or hydergine alone. Atropine sulphate plus scorpion telson extract decreased the volume of gastric juice and acid output while increasing the titratable acidity more than that by scorpion telson extract alone. Macroscopic examination of the stomach in animals receiving hydergine plus scorpion telson extract revealed gross lesions including haemorrhagic erosions and ulcers and destruction of the surface epithelium. Nerves between the gastric glands appeared degenerate. Ganglion cells in the submucosal plexus showed chromatolytic changes in which Nissl bodies were absent. Stomachs from animals receiving hydergine alone did not differ from those of controls.     

ECL cell histamine mobilization studied by gastric submucosal microdialysis in awake rats: methodological considerations

The ECL cells are endocrine/paracrine cells in the acid-producing part of the stomach. They secrete histamine in response to circulating gastrin. Gastric submucosal microdialysis has been used to study ECL-cell histamine mobilization in awake rats. In the present study we assess the usefulness and limitations of the technique. Microdialysis probes were implanted in the gastric submucosa. Histological analysis of the stomach wall around the probe revealed a moderate, local inflammatory reaction 1-2 days after implantation; the inflammation persisted for at least 10 days. Experiments were conducted 3 days after the implantation. The "true" submucosal histamine concentration was determined by perfusing at different rates (the zero flow method) or with different concentrations of histamine at a constant rate (the no-net-flux method): in fasted rats it was calculated to be 87+/-5 (means+/-S.E.M.) nmol/l and 76+/-9 nmol/l, respectively. The corresponding histamine concentrations in fed rats were 93+/-5 and 102+/-8 nmol/l, respectively. With a perfusion rate of 74 microl/hr the recovery of submucosal histamine was 49%, at 34 microl/hr the recovery increased to 83%. At a perfusion rate below 20 microl/hr the microdialysate histamine concentration was close to the actual concentration in the submucosa. The ECL-cell histamine mobilization was independent of the concentrations of Ca2+ in the perfusion medium (0-3.4 mmol/l Ca2+). In one experiment, histamine mobilization in response to gastrin (10 nmol/kg/hr subcutaneously) was monitored in rats pretreated with prednisolone (60 mg/kg) or indomethacin (15 mg/kg). The two antiinflammatory agents failed to affect the concentration of histamine in the microdialysate either before or during the gastrin challenge, which was in accord with the observation that the inflammatory reaction was modest and that inflammatory cells were relatively few around the probe and in the wall of the probe. In another experiment, rats were given aminoguanidine (10 mg/kg) or metoprine (10 mg/kg) 4 hr before the start of gastrin infusion (5 nmol/kg/hr intravenously). Metoprine (inhibitor of histamine N-methyl transferase) did not affect the microdialysate histamine concentration, while aminoguanidine (inhibitor of diamine oxidase) raised both basal and gastrin-stimulated histamine concentrations. We conclude that microdialysis can be used to monitor changes in the concentration of histamine in the submucosa of the stomach, and that the inflammatory reaction to the probe is moderate and does not affect the submucosal histamine mobilization.     

双氢青蒿素抗乳腺癌MDA-MB-231细胞裸鼠移植瘤作用实验研究

目的 研究双氢青蒿素（alpha-Dihydroartemisinin,DHA）对人乳腺癌MDA-MB-231细胞裸鼠移植瘤的抗瘤作用.方法 将裸鼠随机分为阴性对照组、DHA低剂量组（DHA给药剂量为50 mg·kg-1·d-1）、DHA中剂量组（DHA给药剂量为100 mg·kg-1·d-1）和DHA高剂量组（DHA给药剂量为200 mg·kg-1·d-1）,建立人乳腺癌MDA-MB-231细胞裸鼠移植瘤模型,测定不同浓度的DHA对裸鼠的体重、抑瘤率、胸腺指数、脾脏指数的影响,免疫组化检测移植瘤P53蛋白的表达.结果 DHA可明显抑制移植瘤的生长,DHA低剂量、中剂量和高剂量对移植瘤的抑制率分别为20.25%、31.01%和46.20%,DHA各组胸腺指数和脾脏指数较阴性对照组均有不同的的提高,与阴性对照组比较,DHA高、中剂量组小鼠胸腺指数和脾脏指数均有显著性差异（P〈0.05）;免疫组化结果显示DHA可下调移植瘤细胞P53蛋白的表达.结论 DHA对人乳腺癌MDA-MB-231细胞移植瘤有显著的抑制作用,其抗癌机理可能是调节机体免疫和下调P53蛋白的表达.     

A young gastric carcinoma patient with umbilical and scrotal metastasis

A 21-year-old gastric carcinoma patients who showed umbilical and scrotal metastasis is reported. Cutaneous metastases are seen in 3-4% of malignant tumors. The most frequent primary sites are the breast, lung, stomach, ovary, colon, uterus and kidney. Intraabdominal malignancies may produce umbilical metastasis. The primary cancer is usually in the stomach, colon, pancreas or ovary. Umbilical metastasis from the stomach seen in our case is known as Sister Joseph's nodule which is a sign of a poor prognosis. Metastatic scrotal carcinoma is a relatively rare condition which occurs by the hematogeneous pathway. However, in our case, direct invasion of tumor cells though the peritoneal cavity and along the lymphatic system of the spermatic cord was the most likely cause of scrotal metastasis.     

超声诊断胃平滑肌瘤38例分析

目的 探讨胃平滑肌瘤在胃充盈超声下的声像图特征.方法 回顾性分析38例经病理证实平滑肌瘤在胃充盈超声检查中的超声影像资料.结果 特征表现为：黏膜下圆形、类圆形,实性,低回声,边界清楚,回声均匀肿物;肿瘤内少许血流,多见于胃底、体部;大小0.5～5.0 cm,与周围组织界限清楚.胃充盈超声技术对胃平滑肌瘤的诊断符合率85.2%.结论 胃部平滑肌瘤在胃充盈超声下具有特征性,胃充盈超声技术对胃平滑肌瘤诊断有一定价值.     

白细胞介素32在胃癌组织中的表达及与临床病理特征的关系

目的探讨白细胞介素32蛋白在胃癌及其癌旁组织中的表达情况,并分析与临床病理特征之间的相关性。方法采用免疫组织化学法检测胃癌和癌旁组织中白细胞介素32蛋白的表达情况,并结合临床病理资料进行分析。结果胃癌组织中白细胞介素32蛋白的阳性表达率为95%(19/20),明显高于癌旁组织的表达率14.3%(1/7),差异有统计学意义(P<0.05)。白细胞介素32蛋白阳性表达与肿瘤细胞的浸润深度、TNM分期、性别、年龄及有无淋巴结转移无相关性(P>O.05)。结论白细胞介素32在胃癌组织中呈过表达,白细胞介素32蛋白的表达与年龄、性别、肿瘤浸润、肿瘤分期及淋巴转移无关。     

胃肠道小间质瘤55例临床分析

目的：探讨胃肠道小间质瘤临床特点和诊治方法。方法回顾性分析解放军总医院2004年10月-2010年4月接受手术治疗的55例胃肠道小间质瘤患者的临床资料,同时进行随访分析。结果55例中有临床症状者占58.2%;胃部间质瘤占80.0%,其中胃上部间质瘤占52.7%。 Fletcher危险度分级极低和低度占92.7%,肿瘤直径≤1 cm危险度分级更低,Ki-67阳性与危险度分级无关。肿瘤切除采取微创手术者33例（微创手术组）,其中腹腔镜下切除17例,内镜下切除14例,双镜联合局部切除2例;采取传统开放手术者22例（开放手术组）,其中局部切除18例,近端胃切除3例,胰十二指肠切除1例。微创手术组较开放手术组术中出血量及术后开始进食时间差异均有统计学意义（P〈0.01,P〈0.05）。38例获得随访,随访时间26~104个月,中位时间41个月,仅1例胃体间质瘤内镜下切除术后22个月复发。结论胃肠道小间质瘤患者仅半数有临床症状,肿瘤多生长于胃上部,危险度分级及复发风险低,Ki-67不能作为判断胃肠道小间质瘤预后的指标。微创手术治疗安全、有效。     

黑色素瘤细胞外泌体通过Prospero同源异形盒蛋白1调控肿瘤淋巴转移机制的研究

目的 探讨黑色素瘤外泌体(melanoma exosomes,MM-ex)携带的果蝇Prospero同源异形盒蛋白1(prospero homeobox protein 1,Prox-1),在调控淋巴管内皮细胞(lymphatic endothelial cells,LECs)增殖、迁移以及生成具有转移肿瘤细胞功能淋巴管过程中的作用。方法 采用慢病毒转染实验构建低表达Prox-1黑色素瘤细胞株(MM^shProx-1)。采用试剂盒法获取MM和MM-ex?^shProx-1的外泌体(MM-ex和MM-ex^shProx-1)。采用免疫荧光检测、蛋白质印迹法检测、细胞增殖及细胞迁移实验,观察MM-ex及MMex^shProx-1对LECs生物学特性的影响。采用免疫组化(IHC)法检测2015年9月至2021年4月江苏大学附属医院收治的32例皮肤黑色素瘤病理标本中Prox-1及淋巴管内皮细胞透明质酸受体-1(lymphatic vessel endothelial hyaluronic acid receptor...