干细胞因子对红白血病细胞系TF-1凋亡的抑制作用

目的 :探讨干细胞因子 (stemcellfactor ,SCF)阻止红白血病细胞系TF 1进入凋亡程序的作用。方法 :进行3H TdR掺入实验、细胞存活实验、细胞基因组DNA电泳分析和流式细胞术分析。结果 :发现TF 1细胞对SCF的反应存在量 半高效关系 ,在SCF存在时细胞存活率明显提高 ,TF 1细胞系在SCF饥饿 2 4h时出现DNA梯型条带 ;流式细胞术检测显示 ,在SCF饥饿 36h后 ,在G1峰前出现凋亡特有的AP峰。结论 :SCF有阻滞或抑制红白血病细胞系TF 1凋亡的作用。     

Effect of fibrin glue and endothelial cell growth factor on the early healing response of the transplanted allogenic meniscus: a pilot study

Twentyfour meniscal allotransplantations were conducted in 12 adult mongrel dogs. The medial meniscus was replaced using a deep-frozen meniscal allograft. The junction between the meniscus and capsule was treated in one of the three ways. In the control group, the meniscus was sutured only to the adjacent capsular tissue (group C). In the second group, fibrin glue was injected at the junction (group F), and in the third group, fibrin glue and endothelial cell growth factor (ECGF) were injected at the juncture between the transplanted meniscus and the adjacent capsule before the meniscus was sutured (group FE). Histological observation was performed to investigate the effect of fibrin glue and ECGF on the healing process of transplants at various intervals of 1, 4, 8 and 12 weeks. No immunological response was noted in any of the knees. The healing of the transplanted meniscus was first observed at the peripheral attachment. Also, the pannus-like tissue extended from the synovium to the surface of the meniscus. The healing rate in each group at 1 week and 12 weeks was 22% and 77% in group C, 52% and 80% in group F, and 64% and 80% in group FE, respectively. At 4 and 8 weeks, early cellular repopulation was found in group FE and the area which contained new cells was larger than the acellular central core at 8 weeks. However, there was no difference among the three groups at 12 weeks. The early stage of regeneration of the transplanted meniscus seemed to be accelerated by the use of fibrin glue and ECGF; however, they had little influence on the long-term results. Few cells were found in the central portion of the transplanted meniscus even at 12 weeks.     

Computer-aided diagnosis via model-based shape analysis: automated classification of wall motion abnormalities in echocardiograms

RATIONALE AND OBJECTIVE: Shape analysis of endocardial contour sequences from echocardiograms can provide classification of wall motion abnormalities (WMA). MATERIALS AND METHODS: We previously reported on active appearance motion models (AAMM) for automated detection of endocardial contours in sequences of echocardiograms. The shape analysis of AAMM renders eigenvariations of shape/motion, including typical normal and pathologic endocardial contraction patterns. A set of stress echocardiograms (single-beat four-chamber and two-chamber sequences with expert-verified endocardial contours) of 129 infarct patients was split randomly into training (n = 65) and testing (n = 64) sets. AAMMs were generated from the training set and AAMM shape coefficients (ASCs) were extracted for all sequences and statistically related to regional/global visual wall motion scoring (VWMS) and volumetric parameters. RESULTS: Linear regression showed clear correlations between ASCs and VWMS. Discriminant analysis showed good prediction by ASCs of both segmental (74% correctness) and global WMA (90% correctness). Volumetric parameters correlated poorly to regional VWMS. CONCLUSION: 1) ASCs show promising accuracy for automated WMA classification. 2) VWMS and endocardial border motion are closely related; with accurate automated border detection, automated WMA classification should be feasible. 3) ASC shape analysis allows contour set evaluation by direct comparison to clinical parameters.     

The skeletal maturation status estimated by statistical shape analysis: axial images of Japanese cervical vertebra

Objectives:

To evaluate axial cervical vertebral (ACV) shape quantitatively and to build a prediction model for skeletal maturation level using statistical shape analysis for Japanese individuals.

Methods:

The sample included 24 female and 19 male patients with hand–wrist radiographs and CBCT images. Through generalized Procrustes analysis and principal components (PCs) analysis, the meaningful PCs were extracted from each ACV shape and analysed for the estimation regression model.

Results:

Each ACV shape had meaningful PCs, except for the second axial cervical vertebra. Based on these models, the smallest prediction intervals (PIs) were from the combination of the shape space PCs, age and gender. Overall, the PIs of the male group were smaller than those of the female group. There was no significant correlation between centroid size as a size factor and skeletal maturation level.

Conclusions:

Our findings suggest that the ACV maturation method, which was applied by statistical shape analysis, could confirm information about skeletal maturation in Japanese individuals as an available quantifier of skeletal maturation and could be as useful a quantitative method as the skeletal maturation index.     

基于CT平扫纹理分析的影像组学预测肾透明细胞癌WHO/ISUP分级的初步研究

目的:探讨基于肾脏CT平扫图像纹理分析的影像组学模型在预测肾透明细胞癌（ccRCC）WHO/国际泌尿病理学会（ISUP）病理分级中的价值。方法:回顾性分析2016年12月至2019年5月中国科学院大学附属肿瘤医院经手术病理证实且有明确病理分级的90例ccRCC患者,按照7∶3的比例将所有患者随机分为训练组（63例）及测...     

Sex estimation from discriminant function analysis of clavicular and sternal measurements: a forensic anthropological study based on examination of two bones of Northwest Indian subjects

The clavicle and the sternum are important bones of the anterior thoracic skeleton that are commonly found well preserved in forensic contexts. To assess the concurrent suitability of two bones of the same individual in forensic sex estimations, both clavicles and sternums were collected from 252 male and 91 female autopsy cadavers. This study’s bone dimensions were found to be significantly smaller than of subjects of other nationalities and also higher than previous Indian studies. Statistically significant sex differences were noticed in different dimensions of the two bones (p < 0.001). The univariate DFA found that the product index of the clavicle and combined sternal length were the best sex discriminating variables to identify the sex of about 85% of the cadavers. From the multivariate discriminant function analysis of all linear variables (DF: –18.664 + 0.107 (MCI–L) + 0.180 (MCC–R) +.045(MBL) – 0.071(MDL–L), the sex of 90.7% of the cadavers could be estimated correctly and this percentage was highest when either clavicular or sternal measurements were used in the analyses. The concurrent use of the two bones provided higher accuracy levels with lower sex biases than the individual use of either bone in univariate or multivariate analysis. The clavicle was found to be better and more reliable than the sternum for sex estimations in the current autopsy samples. Further studies are needed to corroborate or negate the findings of these investigations.     

抗角蛋白自身抗体对培养人舌鳞癌细胞表达表皮生长因子受体的免疫组化研究

 目的 探讨抗角蛋白自身抗体(AK auto Ab)对培养人舌鳞癌细胞表达表皮生长因子受体(FGFR)的作用.方法 用不同浓度AK aut Ab作用于培养人舌鳞癌细胞后,用免疫组化方法,测鳞癌细胞表达ECFR的变化,并做图像分析.结果 研究发现AK auto Ab对鳞癌细胞EGFR的表达有抑制作用,并呈浓度依赖性,经统计学检验显示有极显著意义(P<0.01).结论 AK auto Ab对鳞癌细胞EGFR的表达有抑制作用.     

Model-based, semiquantitative and time intensity curve shape analysis of dynamic contrast-enhanced MRI: a comparison in patients undergoing antiangiogenic treatment for recurrent glioma

Purpose:

To compare time intensity curve (TIC)‐shape analysis of dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI) data with model‐based analysis and semiquantitative analysis in patients with high‐grade glioma treated with the antiangiogenic drug bevacizumab.

Materials and Methods:

Fifteen patients had a pretreatment and at least one posttreatment DCE‐MRI. We applied a pixel‐by‐pixel TIC shape analysis, where TICs are classified into five different types according to their shape, and calculated the occurrence of each TIC type in the region of interest (ROI). The results were compared to the pharmacokinetic model (PKM) parameters K^trans, K_ep, V_e, and V_i, and with the semiquantitative parameters maximum enhancement (ME) and initial slope of increase (ISI).

Results:

The relative amount of type 2 and 4 TIC shape significantly correlated with the parameter K_ep but not with K^trans or V_e. The PKM parameter V_e and the semiquantitative parameters ME and ISI showed significant changes after treatment. None of the TIC shapes individually showed significant changes.

Conclusion:

The semiquantitative parameters ME and ISI are more sensitive to the effect of the bevacizumab than K^trans and V_e. The pixel‐by‐pixel TIC shape analysis parameters are not sensitive to the effect of bevacizumab, although they can be seen as surrogates for the PKM parameter K_ep. J. Magn. Reson. Imaging 2011;. © 2011 Wiley Periodicals, Inc.