类风湿关节炎患者血清可溶性白介素-2受体水平及临床意义

目的 探讨类风湿关节炎患者血清可溶性白介素-2受体水平及临床意义.方法 用双抗体夹心酶联免疫吸附试验检测30例类风湿关节炎患者血清可溶性白介素-2受体水平.结果 类风湿关节炎患者均显著高于正常对照组.结论 血清可溶性白介素-2受体水平和类风湿关节炎患者病情活动密切相关,是判断预后的一个有用的指标.     

类风湿关节炎患者的免疫活性细胞

目的 研究类风湿关节炎患者免疫活性细胞的活性。 方法 检测外周血Ｔ细胞及其亚群，类风湿因子分类及血清中可溶性白细胞介素２受体含量来研究其活性。 结果 ＣＤ３细胞、ＣＤ４细胞、ＣＤ４／ＣＤ８、ＩｇＭ、ＩｇＡ和ＩｇＧ类风湿因子、血清可溶性白细胞介素２受体含量均高于正常对照组（Ｐ〈０．０５），ＣＤ８细胞无明显变化；同时观察到１５例缓解期患者ＩｇＭ和ＩｇＧ类的类风湿因子及血清可溶性白细胞介素２受体亦高于正     

哮喘儿童血清可溶性白细胞介素－2受体与外周血T细胞亚群检测

哮喘儿童血清可溶性白细胞介素－２受体与外周血Ｔ细胞亚群检测徐琳玲，林小璎，华雪玲，陈珊关键词：哮喘，白细胞介素－２受体／可溶性，Ｔ细胞．亚群，儿童近年来，Ｔ淋巴细胞与细胞因子及其受体在哮喘发病中的作用日益受到重视。为探讨可溶性白细胞介素－２受体（ｓＩ...     

Graves病人血清可溶性白细胞介素－2受体水平的变化

Ｇｒａｖｅｓ病人血清可溶性白细胞介素－２受体水平的变化陈萱林，叶晓芬关键词：Ｇｒａｖｅｓ病，白细胞介素－２受体／可溶性可溶性白介素－２受体（ｓＩＬ－２Ｒ）与一些疾病的病情变化密切相关。本文通过检测Ｇｒａｖｅｓ病患者血清ｓＩＬ－２Ｒ水平并分析临床病情和...     

肾病综合征患者可溶性白介素2受体变化及意义

肾病综合征患者可溶性白介素２受体变化及意义（张道友等．中华内科杂志１９９４；３３（５）：３０９）为了探讨肾病综合征（ＮＳ）患者可溶性白介素２受体（ＳＴＬ－２Ｒ）变化的临床意义，我们检测３６例ＮＳ血清及尿液ＳＩＬ－２Ｒ．结果表明：ＮＳ发作组血清及尿液Ｓ...     

原发性肝癌患者血清可溶性白介素2受体检测及其临床意义

原发性肝癌患者血清可溶性白介素２受体检测及其临床意义［朱瑞龙等．中国肿瘤临床，１９９６；２３（１）：１８］应用夹心酶联免疫吸附法（ＥＬＩＳＡ）检测４５例原发性肝癌（ＰＨＣ）患者及４２例健康成人的血清可溶性白介素２受体（ＳＩＬ２Ｒ）水平，结果显示ＰＨＣ...     

微小病变型肾病患者血尿可溶性白介素2受体的测定及其意义

目的：观察可溶性白细胞介素2受体(sIL—2R)在微小病变型肾病综合征(MCNS)病人血浆和尿液中浓度变化，探讨其在免疫学发病机制中的意义。方法：用ELISA分析法检测23例微小病变型肾病综合征患者发病及缓解期血、尿白介素2受体，同时用^3H—TdR掺入法检测患者淋巴细胞增殖情况。结果：MC—NS患者在发病期血、尿sIL—2Rα明显增高，于缓解期恢复正常。与对照组相比，发病期间患者尿液中增高的白介素2受体与血清浓度不成比例地异常增高。同时尚发现血清可溶性白介素2受体与T细胞体外对PHA的增殖反应呈负相关，缓解期恢复正常。结论：可溶性白介素2受体可能为血清中抑制T细胞克隆扩增的一种因子。     

微小病变型肾病患者血、尿中可溶性白介素Ⅱ受体浓度的变化及意义

目的：观察可溶性白细胞介素Ⅱ受体（sIL-2R)在微小病变型肾病综合征（MCNS）病人血浆和尿液中浓度变化，探讨其在免疫发病中的意义。方法：用ELISA分析法检测23例微小病变型肾病综合征患者发病及缓解期血，蛋白介素Ⅱ受体，同时3H－TdR掺入法检测患者淋巴细胞增殖情况。结果：MCNS患者在发病期血，尿sIL-2Rα明显增高，于缓解期恢复正常，与对照组相比，发病期间患者尿液中增高的白介素II受体与血清浓度不成比较地异常增高，同时还发现血清可溶性白介素II受体与T细胞体外对PHA的增殖反应呈负相关，缓解期恢复正常。结论：可溶性白介素II受体可能为血清中抑制T细胞克隆扩增的一种因子。     

慢性乙型肝炎患者血清SIL—2R及外周血T淋巴细胞亚群检测

应用双抗体夹心法检测了５４例慢性乙型肝炎，肝硬化患者血清可溶性白介素２受体（ＳＩＬ－２Ｒ）应用单克隆抗体和ＡＰＡＡＰ法对Ｔ淋巴细胞亚群进行了检测，结果示慢性乙型肝炎病人血清ＳＩＬ－２Ｒ明显高于对照组，并且其升高与血清胆红素水平相关，而病人ＯＫＴ３细胞与正常对照组无显著差异，ＯＫＴ４阳性细胞比例下降，ＯＫＴ８阳性细胞比例增加，ＯＫＴ／ＯＫＴ比值下降，各临床类型中以重症肝炎ＯＫＴ／ＯＫＴ比值下降显著，     

原发性肝癌患者血清可溶性白介素2受体检测及其临床意义

原发性肝癌患者血清可溶性白介素２受体检测及其临床意义［朱瑞龙等，中国肿瘤临床，１９９６；２３（１）：１８］应用夹心酶联免疫吸附法（ＥＬＩＳＡ）检测４５例原发性肝癌（ＰＨＣ）患者及４２例健康成人的血清可溶性白细胞介素２受体（ｓＩＬ２Ｒ）水平，结果显示Ｐ...     

Soluble interleukin-2 receptor in patients with glomerular diseases.

In this study, we measured the soluble interleukin-2 receptor (sIL-2R) level to evaluate the cellular immune status in 61 patients with different types of glomerular diseases; 40 healthy volunteers were used as control. All patients with glomerular diseases had levels of serum sIL-2R significantly higher than those of the controls (766 +/- 59 vs 280 +/- 23 U/ml; p < 0.05). Even patients with normal renal function still had higher serum sIL-2R levels than the controls, no matter to which subgroups they belonged (primary glomerulonephritis, lupus nephritis or diabetic nephropathy). Serum sIL-2R levels were similar among the three subgroups. The serum levels of sIL-2R correlated well with age and were significantly higher in older patients, although this was not observed in the control group. Serum sIL-2R levels were significantly higher in patients with active urinary sediment and in patients with impaired renal function and showed a significant negative correlation with creatinine clearance (r = -0.56; p < 0.05). Although urinary and serum sIL-2R levels were quite well correlated, (r = 0.35; p < 0.05), the urinary levels of sIL-2R did not differ in patients with different disease activity or different renal functions although they had a significant correlation with 24-hour urinary protein (r = 0.39; p < 0.05). Patients with nephrotic syndrome also had higher urinary sIL-2R levels than other patients (529 +/- 106 vs 280 +/- 31 U/ml; p < 0.05). We conclude that greater T-cell activation might contribute to the pathogenesis of different glomerulonephritis entities, and serum levels of sIL-2R can serve as a useful clinical marker of glomerulonephritis activity. Renal function influenced the serum levels of sIL-2R significantly. This factor must be considered when we interpret the data. Urinary sIL-2R levels did not reflect the disease activity as well. This might be due to the secondary influence of the extent of the glomerular protein leak. Further investigation is needed to define the exact excretory pathway of this substance.     

脑血管病急性期血清sIL-2R水平的检测及其临床意义的初步探讨

目的 :探讨可溶性白细胞介素 -2受体 (sIL -2R)在脑血管病急性期发生、发展中作用。方法 :采用ELISA双抗体夹心法 ,对 5 6例脑血管病急性期患者和 17例正常对照组血清sIL -2R进行了测定。结果 :脑出血组和脑梗塞组急性期sIL -2R水平与对照组相比明显升高 (P <0 .0 1) ;sIL -2R水平与脑出血量无相关性 (r =0 .13 8,P >0 .0 5 ) ;sIL -2R水平随着病情的好转而下降。结论 :sIL -2R可作为判断脑血管病急性期患者病情变化的指标之一。     

Clinical significance of serum CD44 measurement in malignant lymphoma

We monitored the effectiveness of lymphoma therapy by measuring the serum levels of soluble CD44std (sCD44std) and soluble CD44v6 (sCD44v6). Furthermore, we measured the level of soluble interleukin 2 receptor (sIL-2R). A total of 24 patients with non-Hodgkin's lymphoma were enrolled. sCD44std, sCD44v6, and sIL-2R on serum were measured using ELISA system. In all patients, only the sIL-2R level decreased significantly following therapy. However, an analysis of CR and PR showed that the degree of decrease in the sCD44std level was significantly greater than that in the sIL-2R level. Furthermore, among the CS IV cases, only the CD44std level decreased significantly after therapy. These findings suggest that the level of serum sCD44std reflects clinical pathology more closely than the level of serum sIL-2R in CS IV patients and those who respond well to therapy. Moreover, when T-cell and B-cell lymphomas were analyzed separately, the levels of sCD44std and sIL-2R decreased significantly after therapy in patients with B-cell lymphomas, and the degree of decrease in the sCD44std level was very significant with a p-value of 0.0003. This suggests that when sCD44std is used as an index of treatment, it more closely reflects the treatment of B-cell lymphomas. Level of serum sCD44std should prove to be a useful marker for assessing the effectiveness of lymphoma therapy.     

反复自然流产患者血清sIL-2R水平分析

目的 :探讨不明原因反复自然流产 (RSA)患者在不同时期血清sIL - 2R水平。方法 :采用双抗体夹心ELISA法测定了 2 6例非孕期RSA患者 ,2 2例孕期RSA患者 ,8例出现先兆流产的孕期RSA患者 ,2 0例正常妊娠的孕妇与2 1例正常经产妇血清中sIL - 2R水平。结果 :出现先兆流产组血清sIL - 2R水平显著高于其它组 (P <0 .0 1) ,其余 4组间无显著性差异 (P >0 .0 5 )。结论 :血清sIL - 2R水平升高与先兆流产有关 ,RSA患者孕期血清sIL - 2R水平升高应警惕再次发生流产的可能。     

食管癌患者手术前后血清sIL-2R、sIL-6R水平的动态变化

目的:研究可溶性白细胞介素-2受体(sIL-2R)、可溶性白细胞介素-6受体(sIL-6R)在食管癌患者血清中的水平变化,以及sIL-2R与sIL-6R水平在肿瘤临床分期之间的相关关系,并探讨食管癌患者手术前后细胞因子水平的变化。方法:用酶联免疫吸附试验(EL ISA)方法检测了80例食管癌早期(Ⅰ~Ⅱ)患者、58例食管癌晚期(Ⅲ~Ⅳ)患者和120例对照者血清sIL-2R、sIL-6R水平,同时对138例食管癌患者手术前后的两项细胞因子指标进行动态观察。结果:食管癌早期患者组和食管癌晚期患者组血清sIL-2R、sIL-6R水平均显著高于正常对照组(P<0.01),晚期患者较早期升高(P<0.05);食管癌转移组手术后血清sIL-2R、sIL-6R水平比手术前高(P<0.01),无转移组手术后两者水平较手术前明显降低(P<0.01),食管癌患者血清sIL-2R、sIL-6R水平与肿瘤的恶性化程度有明显相关性。结论:sIL-2R在食管癌发病过程中可能起着促进作用,并可以与sIL-6R起协同作用;两者的免疫失调状态可能与食管癌密切相关;sIL-2R、sIL-6 R水平可作为食管癌病情监测的指标,同时对疾病的临床分期、疗效和预后的判断也有一定的帮助。     

皮肌炎患者血清SIL—2R水平变化

目的：探讨血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）在皮肌炎发病机理中的作用。并分析其与血清肌酶的关系。方法：采用双抗体夹心ＥＬＩＳＡ法检测３０例皮肌炎患者皮质类固醇治疗前后及２８例正常对照血清ｓＩＬ－２Ｒ水平。结果：皮肌炎组血清ｓＩＬ２Ｒ水平明显高于正常对照组（Ｐ〈０．００１）,血清ｓＩＬ－２Ｒ水平变化与ＣＰＫ、ＬＤＨ、ＡＬＤ密切相（Ｐ〈０．００１）,关皮质激光治疗后２月血清ｓＩＬ－２Ｒ水平显著降低（Ｐ〉０．０５）。结论：ｓＩＬ－２Ｒ可能在皮肌炎的发病机理中起着重要作用。ｓＩＬ－２Ｒ检测可作为监测皮肌炎活动性的指标。     

Changes and clinical significances of serum soluble interleukin-2 receptor in patients with mechanical trauma

Objective: To study the changes of serum level of soluble interleukin-2 receptor (sIL-2R) in patients with mechanical trauma and the relationship between sIL-2R serum level and injury severity score (ISS), and to explore the mechanism of changes and clinical significances of sIL-2R. Methods: According to the criterion of ISS, 50 patients with mechanical trauma were divided into mild degree group, severe degree group and extremely severe degree group. Twenty-five of those were dynamically observed for 12 d. Blood samples of all the patients were obtained within 24 h, and ond3,d5,d7,d9 and d 12 in dynamically observed patients. The serum concentration of sIL-2R was assayed by ELISA. Results: Level of sIL-2R was significantly higher in patients with trauma than that in healthy persons within 24 h (P<0. 05). Dynamical observation showed curving changes of sIL-2R with increasing in early stage and decreasing later. The climax of sIL-2R appeared earlier in mild group than that in severe degree group and extrem     

SIL-2R在肝癌患者血清中的表达及其临床意义的研究

目的:明确原发性肝癌患者血清中可溶性白细胞介素2受体(SIL—2R)水平与肿瘤的关系。方法:采用双抗体夹心ELISA法.对32例原发性肝癌患者手术前后血清SIL—2R进行检测。结果:1.肝癌患者术前血清SIL—2R水平明显高于健康成人(P<0.01)。2.肝癌肿瘤体积大小与血清SIL—2R水平升高幅度有关(P<0.05)。3.手术后经化疗和介入治疗9个月肿瘤无转移有血清SIL—2R水平低于术前(P<0.05),而有转移者血清SIL—2R水平明显高于术前(P<0.01)。结论:对原发性肝癌患者血清SIL—2R水平测定,不仅能了解肿瘤患者的机体免疫功能状态,且在一定程度上能对疾病的严重程度、疗效及判断预后作出评估。     

儿童肾病综合征细胞免疫功能检测的临床意义

目的　探讨儿童原发性肾病综合征 (NS)淋巴细胞免疫功能的动态变化 ,检测可溶性白介素 2受体 (sIL - 2R)和T细胞亚群 ,阐明其临床意义。方法　应用酶联免疫吸附试验 (ELISA)方法对 2 5例NS活动期和 2 0例缓解期患儿的血清和尿液检测sIL - 2R浓度。应用流式细胞仪检测T细胞亚群的变化。结果　①NS活动期组血清和尿液sIL - 2R浓度分别高于缓解期组 (P <0 .0 1 )和对照组 (P<0 .0 1 ) ;②NS活动期组CD3 、CD4 、CD4 /CD8 比值、NK[CD(1 6 56) ] 细胞均明显低于缓解期组 (P <0 .0 1 )和对照组 (P <0 .0 1 )。结论　NS细胞免疫功能减低 ,血和尿中sIL - 2R浓度的变化可作为肾病综合征活动指标之一。