Pulmonary fat embolism induced by exposure to high ambient temperature in rats with a fatty liver

The aim of this study was to investigate whether a fatty liver contributes to pulmonary embolism under a high ambient temperature. As an experimental model, we exposed fatty liver rats to a high temperature (45°C) and then looked for fat emboli in the alveolar capillaries using the fat-staining method. Fat emboli were detected in the alveolar capillaries of the fatty liver rats, but not in those of the normal liver rats. Moreover, the degree of pulmonary fat embolism tended to become more severe in proportion to the severity of the fatty liver. In addition, fat emboli did not appear at a core body temperature of 40°C, but were detected at a core body temperature of 44°C. From these results, we conclude that a fatty liver may contribute to the formation of pulmonary fat embolism and that high temperatures act as a trigger for the onset of pulmonary embolism. Moreover, it is possible that fatty liver affects the development of heat stroke induced by exposure to a high ambient temperature and that pulmonary fat embolism is a significant finding which helps to enable a diagnosis of heat stroke in autopsy cases.     

Validity and reliability of the Ergomopro powermeter

The aim of this investigation was to assess the validity and reliability of the Ergomopro powermeter. Nine participants completed trials on a Monark ergometer fitted with Ergomopro and SRM powermeters simultaneously recording power output. Each participant completed multiple trials at power outputs ranging from 50 to 450 W. The work stages recorded were 60 s in duration and were repeated three times. Participants also completed a single trial on a cycle ergometer designed to assess bilateral contributions to work output (Lode Excaliber Sport PFM). The power output during the trials was significantly different between all three systems, (p < 0.01) 231.2 +/- 114.2 W, 233.0 +/- 112.4 W, 227.8 +/- 108.8 W for the Monark, SRM and Ergomopro system, respectively. When the bilateral contributions were factored into the analysis, there were no significant differences between the powermeters (p = 0.58). The reliability of the Ergomopro system (CV%) was 2.31 % (95 % CI 2.13 - 2.52 %) compared to 1.59 % (95 % CI 1.47 to 1.74 %) for the Monark, and 1.37 % (95 % CI 1.26 - 1.50 %) for the SRM powermeter. These results indicate that the Ergomopro system has acceptable accuracy under these conditions. However, based on the reliability data, the increased variability of the Ergomopro system and bilateral balance issues have to be considered when using this device.     

Validity and reliability of the SPORTS score

Purpose  

Successful outcomes for an athlete usually consist of returning to their sport. The Subjective Patient Outcome for Return to Sports (SPORTS) score has been recently proposed as an easy to use score for evaluating an athlete’s ability to return to their sport. The aim of this study was to test the reliability and validity of the SPORTS score in athletes 5–10 years after anterior cruciate ligament reconstruction.     

猪腹部火器伤肠穿孔后细菌学及体温的变化

目的:观察腹部火器伤肠穿孔后猪体温变化和伤后细菌学的定量和定性分析为战创伤救治提供理论依据。方法:健康长白仔猪42头随机等分为对照组和伤后1、2、4、8、12和24 h组,实验组建立猪腹部火器伤肠管穿透伤模型后,观察各个时间点的体温变化。进行伤道分泌物的定量和定性分析以及血液的细菌培养及菌群分析。结果:伤后动物体温逐渐升高,在伤后12、24 h实验组的平均体温达40℃以上;伤后8 h,伤道的每克组织细菌数为(5.93±0.78)×105,达到感染的临界值,弹道局部渗出物中均有表面菌群,伤后4 h开始各组可检测到肠道菌群,血液菌培养阳性,可检测到表面菌群和肠道菌群。结论:腹部火器伤肠穿孔后细菌繁殖快、容易入血,体温升高明显,处理应尽早、合理地清创和早期使用广谱抗生素。     

The ingestible telemetric body core temperature sensor: a review of validity and exercise applications

An ingestible telemetric temperature sensor for measuring body core temperature (Tc) was first described 45 years ago, although the method has only recently gained widespread use for exercise applications. This review aims to (1) use Bland and Altman's limits of agreement (LoA) method as a basis for quantitatively reviewing the agreement between intestinal sensor temperature (Tintestinal), oesophageal temperature (Toesophageal) and rectal temperature (Trectal) across numerous previously published validation studies; (2) review factors that may affect agreement; and (3) review the application of this technology in field-based exercise studies. The agreement between Tintestinal and Toesophageal is suggested to meet our delimitation for an acceptable level of agreement (ie, systematic bias <0.1 degrees C and 95% LoA within +/-0.4 degrees C). The agreement between Tintestinal and Trectal shows a significant systematic bias >0.1 degrees C, although the 95% LoA is acceptable. Tintestinal responds less rapidly than Toesophageal at the start or cessation of exercise or to a change in exercise intensity, but more rapidly than Trectal. When using this technology, care should be taken to ensure adequate control over sensor calibration and data correction, timing of ingestion and electromagnetic interference. The ingestible sensor has been applied successfully in numerous sport and occupational applications such as the continuous measurement of Tc in deep sea saturation divers, distance runners and soldiers undertaking sustained military training exercises. It is concluded that the ingestible telemetric temperature sensor represents a valid index of Tc and shows excellent utility for ambulatory field-based applications.     

Validity and reliability of the Fels physical activity questionnaire for children

PURPOSE: The aim was to evaluate the reliability and validity of the Fels physical activity questionnaire (PAQ) for children 7-19 yr of age. METHODS: A cross-sectional study was conducted among 130 girls and 99 boys in elementary (N=70), middle (N=81), and high (N=78) schools in rural Maryland. Weight and height were measured on the initial school visit. All the children then wore an Actiwatch accelerometer for 6 d. The Fels PAQ for children was given on two separate occasions to evaluate reliability and was compared with accelerometry data to evaluate validity. RESULTS: The reliability of the Fels PAQ for the girls, boys, and the elementary, middle, and high school age groups range was r=0.48-0.76. For the elementary school children, the correlation coefficient examining validity between the Fels PAQ total score and Actiwatch (counts per minute) was 0.34 (P=0.004). The correlation coefficients were lower in middle school (r=0.11, P=0.31) and high school (r=0.21, P=0.006) adolescents. The sport index of the Fels PAQ for children had the highest validity in the high school participants (r=0.34, P=0.002). CONCLUSION: The Fels PAQ for children is moderately reliable for all age groups of children. Validity of the Fels PAQ for children is acceptable for elementary and high school students when the total activity score or the sport index is used. The sport index was similar to the total score for elementary students but was a better measure of physical activity among high school students.     

Validity and reliability of the Polar S710 mobile cycling powermeter

The purpose of this study was to determine the validity and reliability of a new mobile bike-powermeter, Polar S710, in laboratory and field conditions, against the SRM crankset. Eight trained subjects performed in a random order six uphill cycling trials of 6-min duration at three different intensities (60 %, 75 % and 90 % of peak power output [PPO]). In addition, 44 other cyclists performed in the laboratory three cycling bouts each of 5-min duration at three different pedal cadences (60, 90 and 110 rpm) at the same absolute intensity (approximately 150 W). Bias between the two devices was correlated (r = 0.79) with the mean power in field conditions; with the S710 reading higher (p < 0.001) by 7.4 +/- 5.1 % than the SRM in the range of power studied. In other words, the mean differences between the two devices increased as the exercise intensity increased. The mean power output obtained with S710 was significantly higher (p < 0.001) by 6.8 +/- 7.9 W (bias x divided-by random error = 1.042 x divided-by 1.049) than the power obtained with SRM in laboratory conditions. Ninety-five percent of the differences of power measured with the S710 ranged between 21.4 W above to 8.3 W below the SRM in laboratory conditions. Mean differences between the two devices increased as the pedalling cadence increased (0.6 +/- 3.8 %, 4.4 +/- 3.7 % and 7.8 +/- 4.4 % at cadence of 60, 90 and 110 rpm respectively). Coefficients of variation in mean power across the four field-based trials at 75 % PPO was 2.2 % and 1.9 % for S710 and SRM, respectively. In conclusion, the S710 recorded power outputs higher than the SRM system in both field and laboratory conditions. Pedalling cadence and exercise intensity influenced differences in mean power. These characteristics make S710 a useful device for recreational cyclists but not for elite cyclists or scientists who require a greater accuracy and validity. However, the limits of the present study (short-term duration testing; single tested variables as intensity, posture, pedalling cadence) require further investigation for generalizing the present results to extensive use in "real world" cycling.     

Validity and reliability of shuttle-run test in Korean adults

The purpose of this study was to evaluate equations of Leger (E1, 1988) and Brewer (E2, 1988) to predict VO (2max) in Korean adults (158 men and 155 women), and reliability tests were randomly conducted among men ( N=90) and women (n=29) in addition to VO (2max) measurements during a graded exercise treadmill test. Both equations significantly underestimated VO (2max) compared to those from the treadmill test ( P<0.01). A strong intra-class correlation between the treadmill result and 20 m shuttle run test was detected (0.86-0.94, P<0.001). 95 percentile limit of agreement of absolute difference in E1 and E2 in both genders were mostly larger than results from the treadmill test. There was a strong correlation between laboratory test and retest but testing hypothesis of equality of means in men showed significant bias ( P=0.003), except difference between test and retest in women ( P=0.05). The test and retest of the upper and lower of absolute difference ± 95% limit of agreement were -4.86 to -19.05 (men) and 13.61 to 27.91 (women). In conclusion, their equations, based on the results, should not be applied for predicting VO (2max) in Korean adults.     

Determination of regional brain temperature using proton magnetic resonance spectroscopy to assess brain-body temperature differences in healthy human subjects.

Proton magnetic resonance spectroscopy ((1)H MRS) was used to determine brain temperature in healthy volunteers. Partially water-suppressed (1)H MRS data sets were acquired at 3T from four different gray matter (GM)/white matter (WM) volumes. Brain temperatures were determined from the chemical-shift difference between the CH(3) of N-acetyl aspartate (NAA) at 2.01 ppm and water. Brain temperatures in (1)H MRS voxels of 2 x 2 x 2 cm(3) showed no substantial heterogeneity. The volume-averaged temperature from single-voxel spectroscopy was compared with body temperatures obtained from the oral cavity, tympanum, and temporal artery regions. The mean brain parenchyma temperature was 0.5 degrees C cooler than readings obtained from three extra-brain sites (P < 0.01). (1)H MRS imaging (MRSI) data were acquired from a slice encompassing the single-voxel volumes to assess the ability of spectroscopic imaging to determine regional brain temperature within the imaging slice. Brain temperature away from the center of the brain determined by MRSI differed from that obtained by single-voxel MRS in the same brain region, possibly due to a poor line width (LW) in MRSI. The data are discussed in the light of proposed brain-body temperature gradients and the use of (1)H MRSI to monitor brain temperature in pathologies, such as brain trauma.     

Validity, reliability and sensitivity of measures of sporting performance

Performance testing is one of the most common and important measures used in sports science and physiology. Performance tests allow for a controlled simulation of sports and exercise performance for research or applied science purposes. There are three factors that contribute to a good performance test: (i) validity; (ii) reliability; and (iii) sensitivity. A valid protocol is one that resembles the performance that is being simulated as closely as possible. When investigating race-type events, the two most common protocols are time to exhaustion and time trials. Time trials have greater validity than time to exhaustion because they provide a good physiological simulation of actual performance and correlate with actual performance. Sports such as soccer are more difficult to simulate. While shuttle-running protocols such as the Loughborough Intermittent Shuttle Test may simulate physiology of soccer using time to exhaustion or distance covered, it is not a valid measure of soccer performance. There is a need to include measures of skill in such protocols. Reliability is the variation of a protocol. Research has shown that time-to-exhaustion protocols have a coefficient of variation (CV) of >10%, whereas time trials are more reliable as they have been shown to have a CV of <5%. A sensitive protocol is one that is able to detect small, but important, changes in performance. The difference between finishing first and second in a sporting event is <1%. Therefore, it is important to be able to detect small changes with performance protocols. A quantitative value of sensitivity may be accomplished through the signal : noise ratio, where the signal is the percentage improvement in performance and the noise is the CV.     

Validity and reliability of selected commercially available metabolic analyzer systems

Automated metabolic gas analysis systems have advanced considerably over the past decade. They provide an abundance of information, which is not possible by using the traditional Douglas bag method and have become an essential tool in both physiological monitoring and in the diagnosis of cardiopulmonary disease. The validity and reliability of the different online metabolic analyzer systems are not well known, with relatively few independent studies being published. The purpose of this review was to examine and evaluate current literature regarding the validity and reliability of commercially available metabolic analyzer systems. This review reveals significant differences between the available systems in the way that they capture and process basic respiratory measurements. Online metabolic analyzer systems were found to vary significantly when compared with Douglas bag methods. These variations have the potential to introduce error into the accuracy with which the health of cardiovascular system can be assessed or training loads can be assigned. Compounding this is the fact that many automated systems are a "black box", which makes it easy to generate data without the user having much understanding of how the data were generated. In conclusion automated metabolic analyser systems are a scientifically robust method for the evaluation of cardiopulmonary function. Individual researchers and clinicians must, however, be able to make their own decisions about the level of error that is tolerable for their individual needs. This presents a significant practical challenge in light of the speed with which technical developments in the field occur and we make some suggestions for the formulation of intersystem comparison studies.     

Validity and reliability of cardiac output by CO2 rebreathing

This article reviews the available literature on the validity and reliability of the non-invasive techniques, commonly known as CO2 rebreathing, for estimating cardiac output. The differing indirect methodologies are described and illustrated. A table, constructed from the available literature, comparing criterion versus estimated cardiac outputs is presented. The varying combinations of methods employed, differing measurement conditions, i.e. rest and exercise, and divergent populations are illustrated and discussed. The correlation between criterion and estimated cardiac output for these studies ranged from r = 0.09 to 0.96, with a % standard deviation of the differences of 1.5 to 176.8%. The Collier and end-tidal methods, in conjunction with either the Comroe or McHardy CO2 dissociation curve appears to be the most established, valid and reliable combination of methods for estimating resting cardiac output. These methods appear to be comparable to the combination of the Defares, end-tidal and Comroe curve methods for estimating cardiac output during exercise. Because of the potential for large errors, caution is urged when interpreting cardiac output results based on indirect estimation for individual assessment, or for subjects with certain types of pulmonary or heart diseases.     

多巴胺受体参与体温调节的研究进展

中枢多巴胺受体包括D1R和D2R两大类，又各有不同的亚型，在机体的体温调节中发挥重要作用，但多巴胺受体不是参与体温调节的惟一因素。本文对多巴胺受体激动剂和拮抗剂的作用特异性进行了重新评价，并总结讨论了两大类多巴胺受体在体温调节中的作用。D1R的活化能够介导体温的下降，而D2R是介导静息体温和稳定体温的主要因素。多巴胺受体之间以及与其他神经递质系统之间存在相互调节和协同作用，共同维持体温平衡。     

Validity and reliability of the Norwegian translation of the Achilles tendon Total Rupture Score

Purpose

Patient reported outcome measures (PROMs) are increasingly being used in clinical research. The Achilles tendon Total Rupture Score (ATRS) is a PROM designed to assess outcomes in patients with ruptures of the Achilles tendon. The aim of this study was to develop a Norwegian adaption of the ATRS and evaluate its validity and reliability.

Methods

The ATRS was translated into Norwegian in accordance with recommended guidelines. The study included 94 patients treated for Achilles tendon ruptures from January 2010 until February 2013, and the web-based questionnaires were administered twice. Reliability was assessed by Cronbach’s alpha, the intraclass correlation coefficient (ICC) and minimal detectable change (MDC). Construct validity was evaluated by calculating the Spearman’s correlation coefficient with the five subclasses of the Foot and Ankle Outcome Score (FAOS), the SF-36 subclass physical function and the SF-36 physical and mental summary scores.

Results

Both internal consistency (Cronbach’s alpha = 0.96) and test–retest reliability (ICC = 0.90) were excellent, and the MDC was 2.12 at the group level and 16.98 at the individual level. Construct validity was supported by Spearman’s correlation coefficients above 0.7 with the SF-36 subclass physical function and the SF-36 physical summary score as well as the FAOS subclasses Sport/Rec and quality of life. There was no floor effect, and 6 patients (6.4%) achieved a maximum score of 100.

Conclusions

The Norwegian adaption of the ATRS demonstrates acceptable validity and reliability for use in the Norwegian population to assess clinical outcomes in patients with Achilles tendon ruptures.

     

Validity and test-retest reliability of the Stride Analyzer in people with knee osteoarthritis

IntroductionSubjects with knee osteoarthritis walk differently compared to healthy subjects. Managing these gait alterations has been proven effective for reducing pain and increasing function. The Stride Analyzer is a low cost gait analysis tool but its clinimetric properties have not been investigated yet in subjects with symptomatic knee osteoarthritis. The aim of this study was to investigate the reliability and validity of the SA compared with the Gold standard (Vicon) in persons with knee OA.MethodsFifteen subjects with symptomatic knee osteoarthritis were instructed to walk at a self-selected speed in a gait laboratory. Temporospatial (TS) gait parameters were recorded simultaneously by the Stride Analyzer and by a 16-camera-infrared optoelectronic motion capturing system (Vicon). Validity and test-retest reliability of the Stride Analyzer were examined by Bland-Altman plots, intra-class correlation coefficients (ICC) and the standard error of measurement (SEM).ResultsTest-retest analyses showed good agreement for all TS parameters with ICC values ranging from 0.805 (single limb support right) to 0.949 (velocity) and SEM% values ranging from 0.78% (stance phase right (% of gait cycle)) to 4.52% (double limb support right (% of gait cycle)). Good agreement between Stride Analyzer and Vicon was found for the following TS parameters: velocity (z = 1.01), cadence (z = −0.85), stride length (z = 1.63) and gait cycle (z = 0.86). All other gait parameters showed lower ICC values (<0.689).InterpretationOur results suggest that the Stride Analyzer can be used in the clinical field to perform gait analysis in subjects with symptomatic knee osteoarthritis.     

Validity and reliability of the Yale Physical Activity Survey in Spanish elderly.

BACKGROUND: The purpose of this study was to validate a Spanish version of the Yale Physical Activity Survey (YPAS) in a population of older adults and to estimate its potential for application in routine physical activity assessment in the elderly. METHODS: A group of 108 (38 men and 70 women) community dwelling, healthy people aged 61-80 yrs responded twice the questionnaire with an interval of two weeks. RESULTS: Test-retest correlations were significant for the total time and energy expenditure. A moderate but also significant agreement was found for the different summary indices. Significant correlations were found for all of the checklist activities and mean differences between the first and second administration did not reach statistical significance for any scores. Total time and energy expenditure correlated positively with Caltrac activity units and negatively with body weight. The activity dimension summary index and the individual index of leisure walk were significantly associated with Caltrac units and negatively with the percentage of body fat. The summary index and the individual index of vigorous activity correlated significantly with the body mass index and the moving index with Caltrac units. A positive association was found between body mass and sitting index. CONCLUSIONS: The Spanish version of the YPAS probed to be a reasonable valid and reliable measure of physical activity in older adults.     

Validity and reliability of the 20 meter shuttle run test in military personnel

is regularly monitored in military personnel, as occupational demands require a certain level of fitness. Distance run (eg, 2 mile) is typically carried out to measure aerobic fitness, but an alternative test could be the 20 meter shuttle run test (20 m SRT). The present study aimed to evaluate validity and reliability of this test in military personnel. An equation for predicting maximal oxygen uptake (VO2max) was developed on 38 Home Guard soldiers and cross validated in 28 Air Force cadets. Reliability of the 20 m SRT, expressed as mean difference in estimated VO2max-- 95% limits of agreement, was -0.8 +/- 3.1 mL x kg(-1) min(-1). Mean difference +/- limits of agreement between estimated and measured VO2max was -0.4 +/- 6.2 mL.kg(-1)x min-'. The 20 m SRT seems to be a reliable test, although validity is less certain, as relatively high variability was observed between measured and estimated VO2max from the 20 m SRT.     

Validity and reliability of activity measures in African-American girls for GEMS

PURPOSE: To determine the reliability and validity of physical activity monitors and self-report instruments suitable for young African-American girls. METHODS: A validation study was conducted by the Girls health Enrichment Multi-site Studies (GEMS) research team to compare an accelerometer with a pedometer and two self-report instruments for assessing physical activity in African-American girls, age 8-9 yr. Girls (N= 68) attended two clinic visits spaced 4 d apart. Each girl wore a MTI/CSA accelerometer (used as the criterion standard for validity) and a pedometer simultaneously for four consecutive days. Girls completed on two occasions a 24-h physical activity checklist of yesterday and usual activities, including sedentary activities (GEMS Activity Questionnaire, GAQ), and a 3-d computerized self-report instrument (Activitygram). RESULTS: Girls were (mean +/- SD) 9.0 +/- 0.6 yr old and had a body mass index of 19.4 kg x m. Reliability measured by intraclass correlations (ICC) and Pearson correlation coefficients (r) were calculated for the MTI/CSA (ICC = 0.37, P< 0.0001), pedometer (ICC = 0.08, = 0.094), Activitygram (ICC = 0.24) (P = 0.005), and GAQ for physical (r = 0.80, P< 0.0001) and sedentary (r = 0.3-0.5, P< 0.005) activities. Significant Pearson correlations between the MTI/CSA and the other instruments, as a measure of validity, were observed for the 4-d average pedometer score (r = 0.47, P< 0.0001), 3-d average Activitygram score (r = 0.37, P= 0.002), and the average of the two yesterday and two usual GAQ activity scores for a subset of 18 physical activities questions (r = 0.27, = 0.03; and r = 0.29,P = 0.02, respectively). The MTI/CSA was uncorrelated with single day scores from the three other instruments. CONCLUSION: The reliability of the instruments tested was acceptable, except the pedometer. Validity correlations were significant when more than one day was used. Self-report instruments need further development for improved reliability and validity.