缺铁性贫血红细胞表面阴离子位点的观察

以聚乙烯亚胺为阳离子探针，标记缺铁性贫血患者红细胞表面阴离子位点，透射电镜观察，图象分析仪定量分析。发现患者红细胞阴离子位点密度较正常人者为少，反应减弱，平均灰度值明显变低。神经氨酸酶处理后阴离子位点少于相应对照组。提示缺铁性贫血患者红细胞表面阴离子位点减少与红细胞的早衰有关。     

再生障碍性贫血患者红细胞表面阴离子位点

采用秀射电镜观察再生障碍性贫血患者红细胞表面的阴离子位点，并用图象分析仪检测阴离子位点的面积和平均灰度值，与正常人红细胞表面阴离子位点比较，再障组红细胞２表面阴离子位点的密度减少，积分光密度明显降低，用神经氨酸酶处理后的表面阴离子位点均少于相应对照组，提示病组红细胞表面阴离子位点的数量减少与红细胞生存期缩短有关。     

缺铁性贫血的病因探讨及对策

目的探讨缺铁性贫血（IDA）的病因及治疗对策。方法对30例缺铁性贫血患者的临床资料进行分析，并观察铁剂治疗后缺铁性贫血患者的血红蛋白、红细胞总数、红细胞平均体积、红细胞平均压积的变化情况。结果铁剂治疗后患者的血红蛋白、红细胞总数、红细胞平均体积、红细胞平均压积恢复正常时间均明显增高。结论月经过多是导致IDA的主要原因，其次是消化系统疾病，但由恶性肿瘤引起IDA也不少见。对非肿瘤引起的缺铁性贫血，铁剂治疗能达到满意疗效。     

贫血诊断与鉴别诊断中应用血液检验的临床研究

目的 探讨贫血诊断与鉴别诊断中应用血液检验的临床效果.方法 选择在我院接受治疗的地中海贫血患者30例、缺铁性贫血患者30例、健康体检者30例作为研究对象,分别设为地中海贫血组、缺铁性贫血组和对照组.对3组进行血液检测,对各组患者的血液指标及检测效果进行比较.结果 地中海贫血组患者红细胞计数、血红蛋白等指标显著高于缺铁性贫血组患者,缺铁性贫血组患者红细胞体积分布宽度显著高于地中海贫血组,缺铁性贫血组患者红细胞体积分布宽度显著高于对照组,对照组患者红细胞平均体积、红细胞平均血红蛋白量、血红蛋白等指标均显著高于地中海贫血组和缺铁性贫血组患者,差异均有统计学意义(P<0.05).地中海贫血组检出率为76.7%,缺铁性贫血组检出率为80.0%,差异无统计学意义(P>0.05).结论 将血液检查应用在贫血诊断中具有明显的效果,可以在临床上推广.     

血常规检测对鉴别缺铁性贫血和地中海贫血的应用价值分析

目的探讨血常规检测对鉴别缺铁性贫血和地中海贫血的临床意义。方法 2014年9月至2016年8月由我院收治的158例缺铁性贫血和地中海贫血患者,其中,86例缺铁性贫血患者,72例地中海贫血患者,对其静脉血进行血常规检测。比较血样中的红细胞分布宽度(RDW)、血红蛋白、红细胞计数和红细胞平均体积(MCV)水平。结果地中海贫血患者的RDW较缺铁性贫血患者更低,而缺铁性贫血患者的血红蛋白和红细胞计数较地中海贫血患者明显更低,2组在MCV这一指标上差异无统计学意义。结论血常规检测可以较好的区分辨别缺铁性贫血和地中海贫血,具有较好的应用价值,值得临床借鉴使用。     

右旋糖酐铁静滴治疗缺铁性贫血9例疗效观察

目的观察右旋糖酐铁静滴治疗缺铁性贫血的疗效。方法静滴右旋糖酐铁，观察缺铁性贫血患者血红蛋白、红细胞总数、红细胞平均体积、红细胞平均压积的变化情况。结果静脉注射右旋糖酐铁后血红蛋白、红细胞总数、红细胞平均体积、红细胞平均压积恢复正常时间明显短于对照组（P〈0.05），输血量较对熙组减少（P〈0.05）。结论静脉注射右旋糖酐铁对治疗缺铁性贫血有肯定疗效，可尽快改善贫血，减少输血量。     

红细胞参数在缺铁性贫血诊疗中的临床意义

目的 探讨平均红细胞体积(MCV),平均红细胞血红蛋白含量(MCH),平均红细胞血红蛋白浓度(MCHC),红细胞体积分布宽度(RDW)等红细胞参数在缺铁性贫血中的变化情况.方法 实验室分别测定试验组和对照组的红细胞参数,并进行两组数据的比较、分析.结果 缺铁性贫血患者的各项红细胞参数均有显著变化.结论 试验结果符合缺铁性贫血的各项参数标准,且RDW在患者体内的显著升高现象为缺铁性贫血的早期发现提供了一条可行捷径.     

红细胞四项参数诊断缺铁性贫血的临床价值

目的 探讨缺铁性贫血患者红细胞参数;平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、红细胞体积分布宽度(RDW)变化情况.方法 用仪器测定115例缺铁性贫血患者MCV、MCH、MCHC、RDW.结果 缺铁性贫血患者MCV、MCH、MCHC、RDW有明显变化.结论 MCV、MCH、MCHC、RDW变化与缺铁性贫血的参数相符.尤其以RDW升高更为敏感,可作为发现早期缺铁性贫血(IDA)筛选的简易指标.     

红细胞参数在缺铁性贫血诊疗中的临床意义

目的 探讨平均红细胞体积(MCV),平均红细胞血红蛋白含量(MCH),平均红细胞血红蛋白浓度(MCHC),红细胞体积分布宽度(RDW)等红细胞参数在缺铁性贫血中的变化情况.方法 实验室分别测定试验组和对照组的红细胞参数,并进行两组数据的比较、分析.结果 缺铁性贫血患者的各项红细胞参数均有显著变化.结论 试验结果符合缺铁性贫血的各项参数标准,且RDW在患者体内的显著升高现象为缺铁性贫血的早期发现提供了一条可行捷径.     

缺铁性贫血红细胞参数变化的临床意义(附123例分析)

目的 :探讨缺铁性贫血患者红细胞参数 :平均红细胞体积 (MCV)、平均红细胞血红蛋白含量 (MCH)、平均红细胞血红蛋白浓度 (MCHC)、红细胞体积分布宽度 (RDW )变化情况。方法 :用仪器测定 12 3例缺铁性贫血患者MCV、MCH、MCHC、RDW。结果 :缺铁性贫血患者MCV、MCH、MCHC、RDW有明显变化。结论 :MCV、MCH、MCHC、RDW变化与缺铁性贫血的参数相符 ,尤其以RDW升高更为敏感 ,可作为发现早期缺铁性贫血 (IDA)筛选的简易指标     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.