共查询到18条相似文献,搜索用时 281 毫秒
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目的: 探讨铁负荷过低上调巨噬细胞、泡沫细胞细胞外基质金属蛋白酶诱导因子(EMMPRIN)表达的机制。研究促分裂原活化的蛋白激酶(MAPK)信号通路、视黄醛x受体(RXR)及过氧化物酶体增殖剂活化受体γ(PPARγ)在此过程中的作用。方法: 将巨噬细胞和泡沫细胞给予MAPK(p38,ERK1/2)信号通路抑制剂及RXR的天然配体预处理,加入或不加入去铁胺继续培养24 h,用Western blot测定细胞中EMMPRIN蛋白的表达。于巨噬细胞和泡沫细胞中加入铁离子鳌合剂去铁胺刺激,用Western blot检测MAPK(p38,ERK1/2)的磷酸化及PPARγ的水平。结果: p38 MAPK通路抑制剂及RXR和配体在本身不影响EMMPRIN表达的同时,可抑制去铁胺对EMMPRIN表达的上调。去铁胺可促进p38 MAPK磷酸化,但不影响PPARγ蛋白表达。结论: p38 MAPK 参与了铁负荷过低上调巨噬细胞和泡沫细胞中EMMPRIN表达的过程;RXR可能参与了该过程。 相似文献
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目的: 探讨核转录因子(NF)-κB在铁负荷过低上调巨噬细胞、泡沫细胞炎症因子反应中的作用。方法: 将巨噬细胞和泡沫细胞给予NF-κB抑制剂预处理,加入或不加入铁离子鳌合剂去铁胺(DFO)继续培养24 h,用Western blot测定细胞中细胞外基质金属蛋白酶诱导因子(EMMPRIN)蛋白的表达。于巨噬细胞和泡沫细胞中加入DFO刺激,用Western blot测定细胞核中NF-κB p65蛋白的表达。将巨噬细胞和泡沫细胞给予p38 MAPK信号通路抑制剂或视黄醛x受体(RXR)的天然配体预处理,加入DFO刺激,用Western blot测定细胞核中NF-κB p65蛋白的表达。结果: NF-κB抑制剂可抑制DFO对巨噬细胞、泡沫细胞中EMMPRIN上调的作用。DFO可促进巨噬细胞、泡沫细胞细胞核中NF-κB p65蛋白的表达。p38 MAPK通路抑制剂或RXR配体可抑制DFO对NF-κB p65蛋白水平上调的作用。结论: NF-κB 参与了铁负荷过低上调巨噬细胞和泡沫细胞中EMMPRIN表达的过程。RXR配体对铁负荷过低上调炎症反应的抑制作用,同其抑制NF-κB激活有关。 相似文献
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Xie J Wang J Li R Dai Q Yong Y Zong B Xu Y Li E Ferro A Xu B 《Journal of molecular and cellular cardiology》2012,53(2):250-258
Syndecan-4 (synd4) is a heparan sulfate proteoglycan, involved in repair following tissue damage, through modulating neovascularization and inflammation. In acute myocardial infarction its myocardial expression is up-regulated in a time-dependent manner, and in synd4-deficient mice severe cardiac dysfunction and abnormal remodeling are observed following induction of myocardial infarction. Here we explored the therapeutic potential of sustained synd4 over-expression in the context of myocardial infarction. Adenovirus containing the synd4 gene (Ad-synd4), or corresponding control adenovirus (Ad-null), was administered intramyocardially in rats immediately after induction of myocardial infarction. Cardiac function was ascertained by echocardiography, hemodynamic assessment and brain natriuretic peptide level 28 days post-intervention. Hearts were excised for molecular and histological analyses at predetermined time points. We observed reduced mortality and improved cardiac function post-myocardial infarction in the Ad-synd4 as compared to the Ad-null group, with associated attenuation of cardiac remodeling, less myocyte loss and reduced fibrosis. Additionally, the Ad-synd4 group exhibited endothelial cell activation and increased angiogenesis and arteriogenesis in the myocardium. The Ad-synd4 group also showed evidence of reduced myocardial inflammation as compared with the Ad-null group, with reduced inflammatory cell (CD45+) and myofibroblast (α-SMA+) infiltration as well as suppressed collagen III deposition and iNOS expression. Our results suggest that sustained synd4 over-expression in the myocardium is of therapeutic benefit following experimental myocardial infarction, through inducing neovascularization, suppressing tissue inflammation and fibrosis, with resultant improvements in cardiac function and remodeling. 相似文献
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Wang Y de Waard MC Sterner-Kock A Stepan H Schultheiss HP Duncker DJ Walther T 《European journal of heart failure》2007,9(6-7):548-557
OBJECTIVE: Infused C-type natriuretic peptide (CNP) was recently found to play a cardioprotective role in preventing myocardial ischaemia/reperfusion (I/R) injury and improving cardiac remodelling after myocardial infarction (MI) in rats. Our study aimed to investigate the effect of cardiomyocyte-specific CNP over-expression on I/R injury and MI in transgenic mice. METHODS AND RESULTS: We generated transgenic (TG) mice over-expressing CNP in cardiomyocytes. Elevated CNP expression on RNA and protein levels was demonstrated by RNase-protection assay and radioimmunoassay. Male TG mice and age-matched wild-type (WT) littermates were subjected to 1-hour global myocardial ischaemia and 23 h of reperfusion or permanent ligation of the coronary artery for 3 weeks. Infarct size did not differ between the WT and TG groups in mice subjected to I/R. In mice that underwent permanent ligation of coronary arteries, both left and right ventricular hypertrophy were prevented by CNP over-expression 3 weeks post-MI. Histological analysis revealed less necrosis, muscular degeneration and inflammation in infarcted TG mice. Impairment of cardiac function was less pronounced in transgenic animals than in the wild-type controls. CONCLUSIONS: Over-expression of CNP in cardiomyocytes does not affect I/R-induced infarct size but prevents cardiac hypertrophy induced by MI. Therefore, CNP may represent a potent therapeutic target for the treatment of patients with cardiac hypertrophy induced by myocardial infarction or other aetiology. 相似文献
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目的 探讨外周输注褪黑素通过下丘脑室旁核(PVN)调节交感神经活性对抗心肌I/R损伤的机制。方法 腹腔注射褪黑素或者无菌生理盐水,24 h后建立C57BL/6J小鼠心肌I/R损伤模型。超声心动图测定心功能,用Evans blue/TTC双染色法测定再灌注24 h后心肌梗死面积。免疫荧光检测下丘脑室旁核(PVN)IL-10和IL-1β及心肌酪氨酸羟化酶(TH)水平;Western blot检测下丘脑室旁核NF-κB水平;ELISA检测血浆去甲肾上腺素(NE)水平。结果 外周给予褪黑素可抑制PVN区NF-κB水平(P<0.01),进而降低PVN区IL-1β水平(P<0.01),升高IL-10水平(P<0.01),降低NE及TH活性,缩小心肌梗死面积(P<0.01),改善C57BL/6J小鼠心功能。结论 外周注射褪黑素,通过调控PVN区炎性细胞因子水平,抑制交感神经活性,改善心肌I/R损伤所致心功能降低。 相似文献
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Xiao J Moon M Yan L Nian M Zhang Y Liu C Lu J Guan H Chen M Jiang D Jiang H Liu PP Li H 《Basic research in cardiology》2012,107(1):239-21
Cellular FLICE-inhibitory protein (cFLIP) is a member of the tumour necrosis factor signalling pathway and a regulator of apoptosis, and it has a role in cardiac remodelling following myocardial infarction (MI) that remains largely uncharacterised. This study aimed to determine the function of cFLIP as a potential mediator of post-infarction cardiac remodelling. Our results show diminished cFLIP expression in failing human and murine post-infarction hearts. Genetically engineered cFLIP heterozygous (cFLIP+/-, HET) mice, cardiac-specific cFLIP-overexpressing transgenic (TG) mice and their respective wild-type (WT) and non-transgenic controls were subjected to MI by permanent ligation of their left anterior descending artery. Cardiac structure and function were assessed by echocardiography and pressure-volume loop analysis. Apoptosis, inflammation, angiogenesis, and fibrosis were evaluated in the myocardium. The HET mice showed exacerbated left ventricular (LV) contractile dysfunction, dilatation, and remodelling compared with WT mice 28 days after MI. Impaired LV function in the HET mice was associated with increases in infarct size, hypertrophy, apoptosis, inflammation, and interstitial fibrosis, and reduced capillary density. The TG mice displayed the opposite phenotype after MI. Moreover, adenovirus-mediated overexpression of cFLIP decreased LV dilatation and improved LV function and remodelling in both HET and WT mice. Further analysis of signalling events suggests that cFLIP promotes cardioprotection by interrupting JNK1/2 signalling and augmenting Akt signalling. In conclusion, our results indicate that cFLIP protects against the development of post-infarction cardiac remodelling. Thus, cFLIP gene delivery shows promise as a clinically powerful and novel therapeutic strategy for the treatment of heart failure after MI. 相似文献
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目的:探讨心肌梗死后小鼠胱天蛋白酶募集域蛋白9(caspase recruitment domain-containingprotein 9,CARD9)的表达及其与炎症的关系。方法:选择野生型小鼠和CARD9敲除小鼠各26只,分为野生型对照组、野生型心肌梗死组、CARD9敲除对照组和CARD9敲除心肌梗死组共4组,每组13只。心肌梗死模型组是通过结扎冠状动脉前降支复制心肌梗死模型,而对照组仅穿线不结扎。于模型复制后第1天,从每组随机抽取3只小鼠处死并行氯化三苯四氮唑(TTC)染色,观察模型是否复制成功;于第3天和第28天,每组分别随机各抽取3只小鼠,处死后取心肌组织行石蜡切片,苏木素伊红(HE)染色和免疫组化染色观察炎症细胞浸润,免疫组化染色和蛋白定量观察CARD9表达。结果:TTC证实心肌梗死模型复制成功;免疫组化染色和蛋白印迹证实,与野生型对照组比较,野生型心肌梗死组3d及28d时梗死区域CARD9表达均增高(P<0.05)与野生型心肌梗死组比较,3 d时CARD9敲除组心肌梗死组巨噬细胞浸润增加(P<0.05)。结论:心肌梗死后CARD9表达上调,而CARD9敲除炎症细胞浸润增加,提示CARD9参与心肌梗死并对早期炎症可能有一定抑制作用。 相似文献