电位滴定法测定安乃近片的含量

目的：建立安乃近片的含量测定方法。方法：以乙醇-0.01 mol/L盐酸溶液（1：1）为溶剂，用电位滴定法测定安乃近片的含量。结果：本法滴定终点突跃明显，重现性好（RSD=0.33%，n=6），平均回收率为99.44%。结论：试验表明该方法简便，快速，专属性强，准确可靠，适用于安乃近片含量的测定。     

电位滴定法测定L—半胱氨酸盐酸盐水合物中氯离子含量

电位滴定法测定Ｌ－半胱氨酸盐酸盐水合物中氯离子含量苏玉永（同济医科大学附属协和医院武汉４３００２２）魏柳珍（湖北省药品检验所）Ｌ－半胱氨酸盐酸盐水合物是制备氨基酸输液的一种重要原料，其结构式中有巯基（－ＳＨ）可以络合银离子，因此不能用银量法测定其氯离...     

电位滴定法测定L—精氨酸的含量

Ｌ－精氨酸属于氨基酸类的一种在我厂生产的精氨酸类头孢拉定原粉中起助溶作用。能否精确测定精氨酸含量，关系到 头孢拉定的可溶性。     

电位滴定法测定苯磺酸左旋氨氯地平的含量

目的：建立测定苯磺酸左旋氨氯地平含量的新方法。方法：采用电位滴定法。结果：该法和国家标准规定的HPLC法测定结果一致。结论：所建方法简便，准确，适用于苯磺酸左旋氨氯地平的含量测定。     

电位滴定法测定磺胺多辛的含量

磺胺多辛即磺胺邻二甲氧嘧啶是磺胺类药物，目前各国药典[1，2，3]都采用重氯化法，以永停满定法（即电流简定法）指示终点。重氯化滴定虽不是氧化还原反应，但当滴定至终点时，溶液中有多余的亚硝酸，电极即起氧化还原反应，使电位产生突跃’‘’，由此，我们利用自动电位滴定仪，对磺胺多辛原料的含量采用电位滴定法指示终点，并与水停法比较，方法快速准确、方便。互．仪器与试剂：METTLERDL25自动电位滴定仪（瑞士），DM140－SC铂金电极，确胶多辛由宁波金露生物工程有限公司提供。2．含量测定：取经IOSC干燥4h样品约O·sg，精…     

电位滴定法测定盐酸麻黄碱原料的含量

目的采用电位滴定法,对盐酸麻黄碱进行含量测定。方法采用氢氧化钠滴定液(0.1 moL/L),用METTLER TOLEDO DL50自动电位滴定仪测定。结果在该条件下,盐酸麻黄碱在0~0.25 g的取样量范围内有良好的线性关系(R~2=1),测得平均回收率为100.33%,标示量的百分含量在100.00%~101.00%。结论本法与2015版《中国药典》方法比较避免了用醋酸汞试液等优点。     

电位滴定法测定盐酸美西律的含量

盐酸美西律为Ib类抗心律失常药,用于急、慢性室性心律失常,如室性早搏、室性心动过速、心室颤动及洋地黄中毒引起的心律失常。盐酸美西律系《中国药典》2005年版二部收载品种,含量测定采用HClO4非水滴定指示剂法,滴定时需加入醋酸汞,有机汞盐对环境和生态的影响很大,容易导致扩大污染的特点,均会对现在与未来的人类生存环境造成影响与威胁。本文建立了电位滴定法。并与原非水滴定指示剂法对照比较,该方法简便快速,易于操作,准确可靠。     

4—氨基—5—咪唑甲酰胺盐酸盐的合成

４－氨基－５－咪唑甲酰胺盐酸盐（１）是重要医药中间体。文献［１］以氰乙酸乙酯（５）为原料,经酯化、氨解和偶联反应制得２－脒基－２－苯偶氮基乙酰胺盐酸盐（２）,再还原制得２－脒基－２－甲酰胺基乙酰胺盐酸盐,在此步反应中使用大量无水乙醚和９５％乙醇,然后在１７０°Ｃ油浴上直接加热环合制备１。该法成本高,纯化困难。文献［２］以２为原料经还原和环合反应制得４－甲酰胺基－５－咪唑甲酰胺盐酸盐,然后酸性水解,用乙醇－乙醚精制得１,收率为２７％。本文参照文献［１～５］,以价廉易得的氯乙酸为起始原料,经氰解、酯…     

电位滴定法测定姜胆咳喘片的氯化铵含量

姜胆咳喘片为由氯化铵、氨苯碱、猪胆粉、乌梅肉、姜、白矾、地龙、陈皮、白芥了组成的中西药复方制剂，原标准收载于《广东省药品标准》(1987年版上册)，无氯化铵的含量测定方法。为了提高质量标准，本文采用电位滴定法测定氯化铵含量，方法可靠，结果准确。     

电位滴定法测定盐酸麻黄碱糖浆的含量

采用电位滴定法测定盐酸麻黄碱糖浆的含量,盐酸麻黄碱在0.02～0.20g范围内线性关系良好,平均回收率为99.8%,RSD为0.08%(n=5),方法简单,精密度高,本法与铁铵矾指示剂法比较,样品的测定结果有显著性差异(P<0.01).     

酸碱双点电位法测定盐酸地尔硫(艹卓)的含量

目的 :建立测定盐酸地尔硫芏卓 含量的新方法。方法 :采用酸碱双点电位滴定法。结果 :该法含量测定和药典法一致。结论 :该法具有所需仪器简单、测定快速、准确等特点 ,可作为盐酸地尔硫芏卓 的质量控制方法。     

在乙醇中用电位滴定法测定依达拉奉原料药含量

OBJECTIVEA method for the determination of edaravone bulk material was established. METHODThe acid-base titration was used, with the solvent of ethanol and the titrating solution of sodium hydroxide standard solution. RESULTSSolved the problem of edaravon     

电位滴定法测定盐酸苯乙双胍含量

目的 建立环保便捷的盐酸苯乙双胍含量的电位滴定方法.方法 采用Mettler Toledo DL53电位滴定仪、Mettler DG113-GC电极,以20mL冰醋酸和20mL醋酐为溶剂进行电位滴定.结果 盐酸苯乙双胍取样量在0.06～0.14g范围内,线性良好,r=0.9999(n=5),中间精密度为0.34%,稳定...     

非水电位滴定法测定盐酸非索非那定原料药含量方法的改进

目的：改进非水电位滴定法测定盐酸非索非那定原料药含量的方法。方法：采用电位滴定法,以冰醋酸-醋酐(1:3)为溶剂,革除现行标准中醋酸汞的使用,建立了测定盐酸非索非那定原料药含量的方法。结果：改进后的方法突跃明显增大,测定结果与高效液相色谱法测定结果无显著差异。结论：改进后的方法不但革除了醋酸汞的使用,且操作简便、快速、重复性好,可用于盐酸非索非那定原料药的含量测定。     

Metabolism of 5-amino-1-beta-D-ribofuranosylimidazole-4-carboxamide and related five-membered heterocycles to 5'-triphosphates in human blood and L5178Y cells.

The facile metabolism of 5-amino-1-β-d-ribofuranosylimidazole-4-carboxamide (AICA ribonucleoside) and several of its structural analogs [5-fluoro-1-β-d-ribofuranosylimidazole-4-carboxamide (FICA ribonucleoside), pyrazofurin and ribavirin] to their corresponding 5′-triphosphates in human blood cells has been demonstrated in vitro. Evidence is presented that both the β- and α-anomeric forms of pyrazofurin nucleotides were present in the extracts of pyrazofurin-treated blood. Determination of the extent of incorporation of radioactivity from [U-¹⁴C]d-glucose into analog ribonucleoside 5′-triphosphates formed in human blood cells indicated that AICA ribonucleoside and ribavirin were metabolized to their 5′-monophosphates mainly (> 90 per cent) via a nucleoside kinase; however, FICA ribonucleoside appeared to be metabolized to its 5′-monophosphate both via a nucleoside kinase (ca. 67 per cent) and via phosphorolytic cleavage followed by a phosphoribosyltransferase-mediated reaction (ca. 33 per cent). The aglycones of AICA ribonucleoside and FICA ribonucleoside were also metabolized extensively to their corresponding ribonucleoside 5′-triphosphates. 5′-Aminoimidazole-4-thiocarboxamide and 3-aminopyrazole-4-thiocarboxamide were metabolized only slightly to their ribonucleoside 5′-triphosphates. [³H]ribavirin was metabolized extensively to its 5′-triphosphate in L5178Y cells but was not detectably incorporated into RNA. Ribavirin caused a substantial decrease in the pool size of GTP in L5178Y cells and a concomitant increase in the pool size of both CTP and UTP.     

Methotrexate and 5-aminoimidazole-4-carboxamide riboside exert synergistic anticancer action against human breast cancer and hepatocellular carcinoma

Aim:

To investigate the influences of methotrexate (MTX) on the anticancer actions and pharmacokinetics of 5-aminoimidazole-4-carboxamide riboside (AICA riboside) in human breast cancer and hepatocellular carcinoma.

Methods:

Human breast cancer cell line MCF-7 and human hepatocellular carcinoma cell line HepG2 were examined. The cell proliferation was assessed using a sulforhodamine B assay. Western blotting and radioactivity assays were used to analyze the phosphorylation of AMPK. The DNA synthesis was analyzed with BrdU incorporation. Nude mice bearing MCF-7 cell xenografts were used to for in vivo study. MTX (50 mg/kg, ip, per week) and AICA riboside (200 mg/kg, ip, every other day) were administered the animals for 2 weeks. The concentrations of AICA riboside and its active metabolite AICA ribotide in the plasma and tumors were measured with HPLC.

Results:

Synergistic cytotoxicity in vitro was observed with MTX (0.1, 0.5, and 1 μmol/L) combined with AICA riboside (0.25–1 mmol/L) in MCF-7 cells, and with MTX (0.5 and 1 μmol/L) combined with AICA riboside (0.5 and 1 mmol/L) in HepG2 cells. MTX (1 μmol/L) significantly enhanced the AICA riboside-induced AMPK activation and BrdU incorporation in both MCF-7 and HepG2 cells. Co-treatment with MTX and AICA riboside exerted more potent inhibition on the tumor growth in nude mice than either drug alone. After injection of AICA riboside (200 mg/kg, iv) in nude mice bearing MCF-7 xenografts, MTX (50 mg/kg, iv) significantly increased the concentrations of AICA riboside and its active metabolite AICA ribotide in tumors.

Conclusion:

MTX and AICA riboside exert synergistic anticancer action against MCF-7 and HepG2 cells in vitro and in vivo. MTX increases the concentration of AICA riboside and its active metabolite AICA ribotide in tumors in vivo.     

电位滴定法测定盐酸特比萘芬的含量

目的建立测定盐酸特比萘芬含量的方法。方法采用非水电位滴定法,以冰醋酸-醋酐为溶剂,用0.1mol·L-1高氯酸滴定液滴定,以玻璃电极为指示电极、饱和甘汞电极为参比电极,用电位法指示终点。结果滴定突跃明显,重复测定同一批样品,其含量的RSD为0.10%(n=6)。结论非水电位滴定法简便快速,准确度高,精密度好,可作为盐酸特比萘芬的含量测定方法 。     

电位滴定法标定高氯酸滴定液及其应用

目的:建立一种用电位滴定法标定高氯酸滴定液的分析方法,并将其结果运用于多种化学原料药的分析。方法:采用电位滴定对高氯酸滴定液(用DG113-SC电极)进行标定,再以相同方法测定盐酸多巴酚丁胺、硝酸硫胺和甘氨酸的含量。结果:用电位滴定法标定,线性关系好(r=0.9999),精确度更高,重复性更好,并且可以避免人为视觉误差。用电位滴定法标定与传统的酸碱指示剂法标定相比,两者之间存在一定差异。将其运用到化学原料药的分析,两种标定方法的结果对含量测定存在系统误差。结论:在药物分析过程中,对于使用电位滴定法测定药品含量的滴定液,应使用电位滴定法来标定其浓度,可以减小方法误差,使测量结果更严密。     

电位滴定法测定注射用益气复脉(冻干)中总有机酸

目的 建立用于测定注射用益气复脉（冻干）（YQFM）中总有机酸含量的电位滴定方法。方法 注射用益气复脉（冻干）用水溶解后,经001*1阳离子交换树脂将盐还原为酸,以琥珀酸为对照品,用电位滴定法测定益气复脉中总有机酸的含量。结果 以琥珀酸计,YQFM中总有机酸滴定精密度RSD 0.42%,稳定性RSD 0.84%,重复性RSD 1.88%,平均加样回收率（n=9）为100.15%,RSD 1.68%。结论 本实验所建立的电位滴定方法简便易行,可操作性强,适用于YQFM中总有机酸的含量测定。     

醇中碱滴定测定盐酸法舒地尔的含量

目的:建立测定盐酸法舒地尔原料含量的无汞污染的容量分析方法。方法:以乙醇为溶剂,用氢氧化钠滴定液(0.1 mol.L-1)滴定,电位法指示终点。结果:该方法与国家标准方法测定结果一致。结论:该方法简便、准确,适用于盐酸法舒地尔原料的含量测定。