非编码RNA与银屑病

银屑病是一种免疫介导的慢性炎症性皮肤病,病因及发病机制涉及遗传、免疫、环境等多种因素。该病以皮肤角质形成细胞过度增殖和异常分化为主要特征。微小RNA(microRNA,miRNA)和长链非编码RNA(long non-coding RNA,lncRNA)均属于非蛋白质编码RNA,广泛参与细胞生命活动和多种人类疾病,在表观遗传水平、转录水平及转录后水平发挥重要的调控功能。近年来研究表明,miRNA和lncRNA与银屑病的发生发展有关。该文将miRNA和lncRNA与银屑病的相关性进行综述。     

银屑病与PRINS lncRNA相关性研究进展

银屑病是一种常见免疫介导的,与遗传、代谢等多种因素相关的疾病,目前病因尚未清晰。近年来越来越多的研究表明,PRINS lncRNA作为一种新型长链非编码RNA在银屑病无损伤的表皮中呈高反应性,参与了G1P3之间的信号传递以及NPM蛋白的调控,其可调节炎症介质产生,在慢性炎症中发挥重要作用。本文就银屑病发病机制与PRINS lncRNA相关性的研究进展作一综述。     

lncRNA LINC01206调控银屑病角质形成细胞的功能研究

 目的探讨长链非编码RNA(lncRNA)在银屑病细胞周期信号通路中发挥的功能及其作用机制。方法 通过GEO数据库收集银屑病转录组测序数据集进行分析,筛选差异表达的基因;通过细胞信号通路富集分析（KEGG）识别调控银屑病的关键信号通路;利用加权基因共表达网络分析（WGCNA）构建lncRNA与蛋白编码基因共表达网络;通过siRNA在人永生化表皮细胞（HaCaT细胞）中敲低LINC01206,流式细胞术检测细胞周期进程。结果差异表达基因富集分析研究发现细胞周期信号通路的失调参与银屑病发病。lncRNA与蛋白编码基因共表达网络分析表明,LINC01206、LINC01269、LINC01215和LINC02159等通过与细胞周期信号通路关键基因CCNA2、CCNB1和CCNE1的相互作用调控银屑病进程。细胞实验结果显示,siRNA在HaCaT细胞中敲低LINC01206,引起细胞周期G2/M的阻滞。结论lncRNA的表达变化引起银屑病细胞周期失调,细胞周期信号通路中的关键lncRNA有望成为银屑病治疗的靶标     

长链非编码RNA在T细胞相关性皮肤病中的作用

长链非编码RNA(lncRNA)是长度＞200个核苷酸且不能编码蛋白的RNA.研究发现lncRNA可以调节T细胞的增殖、分化、凋亡等过程.T细胞在某些皮肤疾病的发病机制中具有重要作用,良性疾病如银屑病、特应性皮炎、白癜风和扁平苔藓,恶性疾病如蕈样肉芽肿、Sézary综合征和间变性大细胞淋巴瘤.本文就lncRNA在T细胞...     

长链非编码RNA在皮肤癌中的研究进展

长链非编码RNA(lncRNA)是一组长度超过200个核苷酸的非编码RNA,近年研究发现,lncRNA在细胞的增殖、分化、凋亡以及肿瘤的发展过程中发挥重要作用。本文就皮肤癌发生和发展相关的lncRNA及其发挥作用的机制进行综述。     

长链非编码RNA与皮肤黏膜疾病

长链非编码RNA(long non-coding RNA,lncRNA)是一种长度超过200个核苷酸的非编码RNA,在生物体的发育,细胞的增殖、分化、凋亡以及肿瘤发生发展中担任着不可或缺的角色。lncRNA在皮肤黏膜疾病发生发展中也起到了重要作用。该文就lncRNA及其在皮肤黏膜疾病中的研究进展作一综述。     

银屑病相关miRNAs表达的研究进展

微RNA(miRNAs)是一种大小约18~25个核苷酸的非编码小分子RNA,具有调控基因表达的作用,参与细胞增殖、分化、凋亡、发育等多种生物学过程,是疾病发生的总"开关"。近年来研究发现多种miRNA在银屑病皮损组织和血浆中有异常表达,是银屑病发生、发展过程中重要的调控因素。本文就这些特异性miRNAs与银屑病发病机制的关系以及在银屑病早期诊断和治疗中的应用做一综述。     

银屑病相关 miRNAs 表达的研究进展

微RNA（miRNAs）是一种大小约18～25个核苷酸的非编码小分子 RNA,具有调控基因表达的作用,参与细胞增殖、分化、凋亡、发育等多种生物学过程,是疾病发生的总“开关”。近年来研究发现多种miRNA在银屑病皮损组织和血浆中有异常表达,是银屑病发生、发展过程中重要的调控因素。本文就这些特异性miRNAs与银屑病发病机制的关系以及在银屑病早期诊断和治疗中的应用做一综述。     

长链非编码RNA在婴幼儿血管瘤中的研究进展

长链非编码RNA(lncRNA)可通过调控染色体重塑、基因转录及蛋白质翻译等影响细胞增殖、迁移及血管生成等多种生物学过程。本文综述近期lncRNA在婴幼儿血管瘤中的研究进展, 总结lncRNA在婴儿血管瘤发生发展中的作用, 以期为其治疗提供新方向。     

寻常性银屑病患者外周血及皮损内MicroRNA-31和MicroRNA-1266的表达水平及临床意义

正寻常性银屑病是一种常见的免疫介导的慢性炎症性皮肤病,以角质形成细胞的增殖和分化异常为特点,在全球发病率为2%～3%~([1])。Micro RNA(miRNA)是一种高度保守的内源性非编码RNA,可通过调节角质形成细胞的分化、增殖和细胞因子反应,在银屑病发病过程中起重要作用~([2-3])。近年来的研究发现,miR-31及miR-1266在固有免疫、炎症性皮肤病及恶性肿瘤发生等各种生物学过程中均发挥着重要的调控作用~([4-5])。然而,     

Dermatology in private practice in France in 2000

OBJECTIVES: Data regarding French dermatological practice are scarce. Our objective was to identify the skin disorders most commonly diagnosed by office-based dermatologists. We also documented the severity of these skin disorders, as reflected by the repercussions on patient's everyday life, and the way physicians managed patients. DESIGN: We carried out a one-day survey of visits to a randomly selected sample of 900 French office-based dermatologists. The randomization was stratified according to the five French different dialing area codes. RESULTS: Office-based dermatologists saw 6411 patients with 7839 skin disorders during the survey. The daily number of visits to French dermatologists was estimated at 47 000 and the annual number between 12 and 14 millions. Office-based dermatologists mostly managed warts, acne, nevus, dermatitis, malignancies and pre-malignancies, fungal infection and psoriasis. Repercussions on patients'everyday life were assessed by physicians as important or very important in 28 p. 100 of cases. Half of the patients received topical treatment, 20.5 p. 100 a systemic drug and 40 p. 100 a minor surgical procedure (including cryotherapy). CONCLUSION: Although dermatologists frequently see benign skin disorders such as warts or nevus, more severe diseases represent an important part of their activity.     

Pentoxifylline in reactions in leprosy

A study was conducted to assess the response of reactions in leprosy to pentoxifylline therapy. Ten cases were studied; 8 cases had type 2 reaction and 2 cases had type 1 reaction. Pentoxifylline was given orally 400 mg three times daily. In patients with type 2 reaction, good response was observed within one week. There was near complete regression of ENL lesions within one month. Cases with type 1 reaction did not respond to pentoxifylline.     

Leukocyte migration in vivo and in vitro in patients with psoriasis

Leukocyte migration in vivo was studied with a skin chamber technique in 21 patients with active psoriasis vulgaris and 18 with cleared psoriasis vulgaris. Measuring over 24 h, no difference was found between healthy volunteers and most patients with active psoriasis, although a subgroup of patients with long-lasting relapses showed subnormal migration values. In patients with cleared psoriasis on the other hand the in vivo leukocyte migration values were increased. In addition, leukocyte migration in vitro under agarose was studied, but no difference was found between healthy controls and patients with psoriasis, active or cleared.     

Decrease in neutrophils observed in vivo in psoriatics after PUVA therapy

Summary In 56 patients leucocyte and differential counts were done before and at weekly intervals during PUVA treatment of chronic recalcitrant psoriasis. A statistical significant (P<0.01) decrease in the percentage of neutrophils was observed during the first week of the PUVA therapy. This observation could be closely related to the clinical clearing of psoriasis (P=0.02).The effect of PUVA therapy in psoriasis may be due to a decrease in the number of immunocompetent neutrophils demonstrated in psoriatic lesions.