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1.
El-Haddad MA Ismail Y Guerra C Day L Ross MG 《American journal of obstetrics and gynecology》2003,189(4):949-952
OBJECTIVE: In adults, nutrient intake is controlled by opposing actions of appetite stimulants (eg, neuropeptide Y [NPY]) and suppressors (eg, leptin). Because NPY may exert a preferential role in mediating adult carbohydrate intake, we sought to determine the effect of central NPY on near-term fetal carbohydrate ingestion. STUDY DESIGN: Five pregnant ewes and fetuses were prepared with fetal vascular, sublingual, and intracerebroventricular catheters, electrocorticogram, and esophageal electromyogram electrodes and studied at 131+/-2 days' gestation. After a 2-hour baseline period, 10% sucrose was infused sublingually for the duration of the study. At 4 hours' time, NPY was injected into the fetal cerebral ventricles and fetal swallowing monitored for an additional 6 hours. RESULTS: During the basal period, mean (+/-SEM) swallowing averaged 0.8+/-0.1 swallows per minute. Fetal swallowing increased significantly in response to sublingual sucrose (1.3+/-0.1 swallows/min, P=.001), and further significantly increased at 4 to 6 hours after NPY injection into the cerebral ventricles (1.8+/-0.3, P=.001). CONCLUSION: These results indicate central NPY stimulation of fetal ingestion beyond that resulting from sublingual 10% sucrose. The in utero development of NPY-induced ingestive behavior may be in preparation for high neonatal caloric intake. 相似文献
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El-Haddad MA Chao CR Ma Sx Ross MG 《American journal of obstetrics and gynecology》2000,182(3):713-719
OBJECTIVE: Human and ovine fetuses demonstrate an enhanced rate of spontaneous and angiotensin II-stimulated swallowing. Angiotensin II and nitric oxide synthase have been localized to thirst centers in the brain. This study was performed to determine whether central nitric oxide contributes to the regulation of angiotensin II-induced fetal swallowing. STUDY DESIGN: Six pregnant ewes with near-term singleton fetuses were chronically prepared with fetal vascular and lateral ventricle catheters and electrocorticogram and esophageal electromyogram electrodes. After a 2-hour control period, fetuses were administered serial lateral ventricle injections (1 mL) of angiotensin II (3.2 microg; time, 2 hours) and N omega-nitro-L -arginine methyl ester (3 mg; time, 3 hours) and a repeat angiotensin II injection (3.2 microg; time, 5 hours). All fetuses received an additional control study of lateral ventricle injections of artificial cerebrospinal fluid on a previous day. RESULTS: Angiotensin II injection significantly increased mean +/- SEM fetal swallowing (0.9 +/- 0.1 to 2.7 +/- 0.4 swallows/min). N omega-nitro-L -arginine methyl ester significantly decreased fetal swallowing to below the basal rate (0.4 +/- 0.1 swallows/min), and swallowing did not increase with the second angiotensin II dose (in the presence of nitric oxide blockade). CONCLUSIONS: These results demonstrate that inhibition of central nitric oxide suppresses fetal swallowing behavior in response to central angiotensin II. We speculate that tonic nitric oxide facilitates angiotensin II swallowing stimulation by maintenance of glutamate activation of hypothalamic N -methyl-D -aspartate receptors. 相似文献
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MA El-Haddad Y Ismail L Day MG Ross 《The journal of maternal-fetal & neonatal medicine》2013,26(5):291-296
Objective: Leptin is produced in adipocytes and is present in the term fetus. In the adult, leptin acts centrally to inhibit neuropeptide Y-induced carbohydrate intake. We sought to examine if central leptin alters fetal ingestion of oral sucrose in the near-term ovine fetus.Methods: Five pregnant ewes and fetuses were prepared with fetal vascular, sublingual and intracerebroventricular (ICV) catheters and esophageal electromyogram electrodes, and studied at 132?±?1 days' gestation (term 145–150 days). Following a 2-h baseline period, 10% sucrose was infused sublingually (0.25?ml/min) for the duration of the study. At time 4?h, leptin (0.075?mg/kg) was administered ICV and fetal swallowing was monitored for an additional 6?h.Results: During the basal period, fetal swallowing averaged 0.7?±?0.1 swallows/min. Fetal swallowing increased significantly in response to 10% sucrose (1.2?±?0.1 swallows/min; p?<?0.05). In response to ICV leptin, fetal swallowing remained significantly elevated at 2, 4 and 6?h (1.3?±?0.4, 1.4?±?0.3 and 1.5 ±?0.2 swallows/min, respectively; p?<?0.05 vs. control).Conclusions: These results indicate that central leptin inhibition of sucrose ingestion is not functional in the near-term fetus. We speculate that a leptin-mediated anorexigenic response is not present at birth, such that unopposed appetite stimulatory mechanisms in the newborn may facilitate rapid newborn weight gain despite high body fat levels. 相似文献
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M A El-Haddad Y Ismail L Day M G Ross 《The journal of maternal-fetal & neonatal medicine》2004,15(5):291-296
OBJECTIVE: Leptin is produced in adipocytes and is present in the term fetus. In the adult, leptin acts centrally to inhibit neuropeptide Y-induced carbohydrate intake. We sought to examine if central leptin alters fetal ingestion of oral sucrose in the near-term ovine fetus. METHODS: Five pregnant ewes and fetuses were prepared with fetal vascular, sublingual and intracerebroventricular (ICV) catheters and esophageal electromyogram electrodes, and studied at 132 +/- 1 days' gestation (term 145-150 days). Following a 2-h baseline period, 10% sucrose was infused sublingually (0.25 ml/min) for the duration of the study. At time 4 h, leptin (0.075 mg/kg) was administered ICV and fetal swallowing was monitored for an additional 6 h. RESULTS: During the basal period, fetal swallowing averaged 0.7 +/- 0.1 swallows/min. Fetal swallowing increased significantly in response to 10% sucrose (1.2 +/- 0.1 swallows/min; p < 0.05). In response to ICV leptin, fetal swallowing remained significantly elevated at 2, 4 and 6 h (1.3 +/- 0.4, 1.4 +/- 0.3 and 1.5 +/- 0.2 swallows/min, respectively; p < 0.05 vs. control). CONCLUSIONS: These results indicate that central leptin inhibition of sucrose ingestion is not functional in the near-term fetus. We speculate that a leptin-mediated anorexigenic response is not present at birth, such that unopposed appetite stimulatory mechanisms in the newborn may facilitate rapid newborn weight gain despite high body fat levels. 相似文献
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The transient relationship between Braxton Hicks' contractions and fetal motor behavior was studied in 14 healthy nulliparous women near term. Two-hour recordings of fetal heart rate and uterine contractions and of real-time scanning for fetal body movements, breathing, and eye movements were made. The recordings were divided into state 1F and non-state 1F. Braxton Hicks' contractions were not influenced by fetal behavioral states and state changes were not related to these contractions. Fetal body movements did not stimulate contractions, but contractions coincided with a specific clustering of body movements during the ascending part of contractions. Breathing was clustered during the descending part of short-lasting contractions but decreased gradually during the long-lasting ones. Heart rate variation was increased during contractions. 相似文献
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Rocha E Totten S Hammond R Han V Richardson B 《American journal of obstetrics and gynecology》2004,191(2):497-506
OBJECTIVE: The purpose of this study was to determine the immunoreactivity of selected structural proteins in the preterm and near-term ovine fetal brain and the response to intermittent umbilical cord occlusion as a measure of altered cellular growth. The intermediate filament proteins nestin, vimentin, and glial fibrillary acidic protein was used as markers for astroglial maturation and astrogliosis, and myelin basic protein as a marker for oligodendrocytes and myelin formation. STUDY DESIGN: Fetal sheep (control and experimental groups at 0.75 and 0.90 of gestation) were studied over 4 days; umbilical cord occlusion was performed in the experimental group by complete inflation of an occluder cuff for 90 seconds every 30 minutes for 3 to 5 hours each day. Animals were then killed, and the fetal brain was perfusion fixed and processed for immunohistologic examination of the gray and white matter. Immunoreactivity was quantified with an image analysis system and expressed as the fractional area positive stain for each protein. RESULTS: In both preterm and near-term animal groups, umbilical cord occlusion caused a large decline in arterial Po(2) (to approximately 7 mm Hg), a modest decline in pH (to approximately 7.30), and a modest rise in Pco(2) (to approximately 61 mm Hg; all P <.01), with a return to control values after the occluder release and no cumulative acidosis over each day of study. Vimentin and glial fibrillary acidic protein immunoreactivity showed reciprocal changes, with vimentin decreased and glial fibrillary acidic protein increased in both the gray and white matter of the control group from 0.75 to 0.90 of gestation, which can be attributed to the transition of radial glia into mature astrocytes. Myelin basic protein immunoreactivity increased approximately 3-fold in the white matter of the control group with advancing gestation, which likely reflected active oligodendrocyte differentiation and increased myelination at this time of development. Intermittent umbilical cord occlusion over 4 days resulted in an approximately 60% decrease in nestin, vimentin, and glial fibrillary acidic protein immunoreactivity, which was qualitatively similar for both the gray and white matter and likely indicative of altered protein synthesis and/or degradation, but only in the preterm group and with no change in myelin basic protein immunoreactivity. CONCLUSION: There is considerable change in the immunoreactivity of structural proteins within the ovine fetal brain over the latter part of gestation and consistent with a high rate of protein turnover, as previously reported. Intermittent umbilical cord occlusion as studied with minimal evidence for necrotic cell injury appears capable of altering selected protein synthesis/degradation, more so in younger animals when protein turnover is higher, which might then impact on the brain's development. 相似文献
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Cerebral blood flow and metabolism in relation to electrocortical activity with severe umbilical cord occlusion in the near-term ovine fetus 总被引:2,自引:0,他引:2
Kaneko M White S Homan J Richardson B 《American journal of obstetrics and gynecology》2003,188(4):961-972
OBJECTIVE: The purpose of this study was to determine the change in cerebral blood flow and substrate metabolism in relation to electrocortical activity in the near-term ovine fetus with repeated umbilical cord occlusion of a severe degree. STUDY DESIGN: Eight near-term fetal sheep were studied through a 2-hour control period, a 6-hour experimental period with repeated cord occlusion of 4 minutes' duration every 90 minutes, and a 16-hour recovery period. Regional cerebral blood flow was measured with the microsphere technique before, during, and after the first cord occlusion; blood flow in the superior sagittal sinus, the cerebral perfusion pressure, and the electrocortical activity were monitored continuously. Brachiocephalic arterial and sagittal venous blood were sampled at selected time points for blood gas and pH, oxygen content, and glucose and lactate levels. RESULTS: Severe umbilical cord occlusion as studied resulted in profound hypoxemia with modest hypercapnia and acidemia, to a similar degree with each insult, but with a return to preocclusion values after occluder release. Glucose values also fell acutely with each cord occlusion by approximately 30% but showed an overall increase through the experimental period, from 0.80 to 1.44 mmol/L; lactate values showed an increase, from 1.21 to 6.10 mmol/L (both P 1 <.01). Fetal electrocortical activity was disrupted markedly, with an abrupt flattening of the electrocortical amplitude by 1.5 minutes of each cord occlusion on average and with an overall increase in indeterminate state activity during the experimental and through the recovery periods. Cerebral blood flow increased approximately 2.5- and 2.8-fold, as measured at 2 and 3.5 minutes during the first cord occlusion (both P <.01) and with the regional flow increase greater in the subcortex and brainstem. Cerebral extraction of oxygen fell toward zero, as measured at 2 minutes during the second and fourth occlusions (P <.05) with oxygen uptake no longer measurable; glucose extraction was now increased approximately 2-fold (P <.05), which indicates that anaerobic metabolism of glucose must be the predominant source of energy at this time. Superior sagittal sinus blood flow also increased in all animals, approximately 1.4- and 1.6-fold at 2 and 3.5 minutes of the first cord occlusion, but much less than the corresponding increase in arterial inflow; the increase was in response to subsequent occlusions was further reduced. CONCLUSION: Severe umbilical cord occlusion in the near-term ovine fetus results in a rapid decrease in the availability of oxygen to the brain. The low PO (2) gradient from blood to tissue rate limits for oxygen consumption by 2 minutes of insult (despite the marked increase in blood flow) and signals the shift to anaerobic metabolism, the suppression in electrocortical activity, and the probable shutdown of other energy-using processes. 相似文献
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We report a near-term fetus with primary acalvaria, an extremely rare malformation characterized by the complete absence of the membranous neurocranium. Sonographic findings were considered consistent with this condition, which was confirmed by magnetic resonance imaging. Spontaneous vaginal delivery resulted in a fresh stillbirth, with an intact cranial sac. 相似文献
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The effects of fetal thyroidectomy in the ovine fetus 总被引:1,自引:0,他引:1
A Bhakthavathsalan L I Mann J Ayromlooi W Kunzel M Liu 《American journal of obstetrics and gynecology》1977,127(3):278-284
Cerebral function and metabolism, morphology and histology of the nervous system, and lung maturity in terms of surfactant production were studied at term in five sheep fetuses thyroidectomized at gestational ages ranging from 104 to 111 days. Compared to age-matched controls, body weight (thyroidectomized animals, 2.8 +/- 0.11 kilograms; controls, 3.9 +/- 0.2 kilograms; P less than 0.0005) and brain weight (thyroidectomized animals, 43.51 +/- 1.38 grams; controls, 48.49 +/- 1.75 grams; P less than 0.05) were significantly reduced. In the nervous system, morphology, histology, brain function, and metabolism were not significantly different from those of the controls. Lung weight was less, but not significantly, and surfactant production was not deficient as compared to that of age-matched controls. The failure to find any disturbance of maturation in the brain and lungs of fetuses thyroidectomized at 104 to 111 days may be related to the relative maturity of the sheep fetus at this age. 相似文献
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Effect of variable long-term maternal feed allowance on the development of the ovine placenta and fetus 总被引:1,自引:0,他引:1
Quigley SP Kleemann DO Walker SK Speck PA Rudiger SR Nattrass GS DeBlasio MJ Owens JA 《Placenta》2008,29(6):539-548
Maternal feed allowance during pregnancy can affect the development of the ovine placenta and fetus. The impact of variations in feed allowance prior to as well as throughout pregnancy has received less attention. Ewes were offered 0.6 (R), 1.2 (C) or 1.8 (AL) maintenance requirements from 89 days before conception until day 133 of pregnancy. Ewes were euthanised on days 50, 92 and 133 of pregnancy. Ewe live weight and body condition score, maternal and fetal metabolic and hormonal profiles, fetal body dimensions and organ weights, and the number, weight and morphology of placentomes were measured. Maternal live weight and condition score were lower in R compared to AL ewes at all stages of pregnancy (P<0.05). Plasma glucose and albumin concentrations of R ewes were significantly reduced (P<0.05) at mid and late gestation, respectively. Placental components were generally unresponsive to long term variations in maternal feed allowance. However, placental weight was significantly (P<0.05) correlated with fetal weight at days 50 (r=0.59) and 133 (r=0.69) of gestation. By late gestation growth-retarded singleton fetuses from R ewes were 19% lighter (P<0.05), with reduced abdominal (9%) and thoracic (10%) girths (P<0.05) but of similar crown-rump length compared with fetuses from AL ewes. These differences were associated with significantly reduced IGF-I concentrations in fetal plasma (P<0.05). In conclusion, maternal, placental and fetal adaptations to long established planes of variable maternal feed allowance were able to maintain fetal growth during early and mid-pregnancy while fetal growth restriction, associated with reduced fetal IGF-I levels, became apparent in late pregnancy. 相似文献
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OBJECTIVE: We sought to investigate the effect of a decrease of maternal plasma osmolality produced by hypotonic rehydration on the fetal urine production rate in normal near-term human fetuses. STUDY DESIGN: Twenty-one healthy pregnant women attending the clinic for antenatal care were studied between 37 and 40 weeks' gestation. The fetal urine production rate was assessed by serial measurements of 3 diameters of the fetal bladder. The hourly fetal urine production rate was determined by linear regression analysis of the calculated bladder volumes versus time and was initially determined after a period of 4 hours of fluid deprivation. Thereafter, the women were asked to drink 1 L of water, and the hourly fetal urine production rate was assessed again. The hourly fetal urine production rate was only studied during behavioral state 1F because it is dependent on the behavioral state. The fetal behavioral state was determined by assessment of fetal heart rate, fetal eye movements, and fetal body movements. RESULTS: Successful recordings were obtained in 10 of the 21 women. The hourly fetal urine production rate increased significantly after hypotonic rehydration (P <.02). Compared with the initial hourly fetal urine production rate after 4 hours of fluid deprivation, the hourly fetal urine production rate showed an increase of 63.2% after hypotonic rehydration, from 38.2 +/- 16.3 mL/h to 62.4 +/- 34.6 mL/h (mean +/- SD). After rehydration, the baseline fetal heart rate fell significantly, from 141 +/- 6 to 132 +/- 8 beats/min (mean +/- SD; P =.005). CONCLUSION: The fetal urine production rate is augmented after acute maternal oral hypotonic rehydration after 4 hours of fluid deprivation. The current findings demonstrate that the near-term human fetus can handle such acute changes in fluid osmolality by increasing the urine production rate to maintain its fluid homeostasis. This mechanism implies that changes in maternal plasma osmolality and volume probably play an important role in determining amniotic fluid volume. Therefore the application of maternal hydration for the treatment of oligohydramnios should be further investigated. 相似文献
14.
Deruelle P Houfflin-Debarge V Magnenant E Jaillard S Riou Y Puech F Storme L 《American journal of obstetrics and gynecology》2003,189(1):208-215
OBJECTIVES: Although mechanisms of glucocorticoids-induced parenchymal lung maturation have been largely studied, little is known about the pulmonary vascular effects of antenatal glucocorticoids (GCs). We therefore hypothesized that antenatal GCs may alter the hemodynamic response to vasodilatory agents in the fetal lung. STUDY DESIGN: We tested the hemodynamic response to acetylcholine, increased PaO(2), and norepinephrine infusion before and after maternal GC administration in chronically prepared, late-gestation fetal lambs (135-137 days of gestational age, term = 147 days). RESULTS: We found that antenatal GCs (1). do not change the basal pulmonary vascular tone and (2). do not alter the vasodilatory response to acetylcholine and increased PaO (2) but enhanced the norepinephrine-mediated pulmonary vasodilation. CONCLUSION: Our results indicate that antenatal GCs alter the pulmonary vascular reactivity to catecholamines. We speculate that the benefits of antenatal GCs on the cardiovascular adaptation at birth may be related to potentiation of catecholamines vascular effects. 相似文献
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Vargas VE Kaushal KM Monau T Myers DA Ducsay CA 《Reproductive sciences (Thousand Oaks, Calif.)》2011,18(3):277-285
This study was designed to determine the potential mechanism/mechanisms of previously observed enhanced fetal cortisol secretion following exposure to long-term hypoxia (LTH). Pregnant ewes were maintained at high altitude (3820 m) for approximately the last 100 days of gestation. Between the gestation days of 138 and 141, adrenal glands were collected from LTH and age-matched normoxic control fetuses. Cyclic adenosine monophosphate (cAMP), cortisol, and steroidogenic acute regulatory (StAR) protein were measured in response to adrenocorticotropic hormone (ACTH) stimulation. Cortisol responses to ACTH were also measured in the presence of the protein kinase (PKA) inhibitor H-89, proopiomelanocortin (POMC), or 22-kDa pro-ACTH. Cortisol output was higher in the LTH group compared to the control (P < .05), following ACTH treatment while the cAMP response was similar in both groups. Although PKA inhibition decreased cortisol production in both groups, however no differences were observed between groups. Western analysis revealed a significant increase in protein expression for StAR in the LTH group (P < .05, compared to control). Proopiomelanocortin and 22-kDa pro-ACTH did not alter the cortisol response to ACTH treatment. Results from the present study taken together with those of previous in vivo studies suggest that the enhanced cortisol output in the LTH group is not the result of differences in cAMP generation or PKA. We conclude that enhanced cortisol production in LTH adrenals is the result of enhanced protein expression of StAR and potential downstream signaling pathways. 相似文献
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Braaksma MA 《European journal of obstetrics, gynecology, and reproductive biology》2001,94(2):186-188
Mild to moderate hypoxemia without major changes in pH and pCO(2) does not reduce fetal renal blood flow and fetal urine production rate. Other factors such as acidemia, hypercapnia or changes in lung liquid production or fetal swallowing are candidates for the reduced amniotic fluid in the growth retarded fetus. Mild hypoxemia influences the fetal brain development in that the migration of PKC immunoreactive cells is delayed after a period of 48 h of hypoxemia. This could be due to the fact that under these circumstances the expected compensatory increase in fetal cerebral blood flow was only significant 1 h after the onset of hypoxemia. Further research on the final position of the hippocampal neurons in term lambs, subjected to fetal mild hypoxemia, can give more information on the effects of hypoxemia on the fetal brain. 相似文献
20.
Kim SJ Cheung CY Widness JA Brace RA 《Journal of the Society for Gynecologic Investigation》2002,9(2):75-79
OBJECTIVE: In previous studies of ovine fetuses, plasma erythropoietin (EPO) concentration increased 10- to 20-fold at 24 hours after hemorrhage and returned toward normal at 48 hours. The present study in fetal sheep was designed to determine in detail the time course of the hemorrhage-induced changes in plasma EPO levels and explore the relationships among EPO concentration, blood oxygen tension, and hematocrit. METHODS: Chronically catheterized, late gestation ovine fetuses (n = 12) were hemorrhaged such that 40% of their blood volume was lost over 2 hours. Plasma EPO concentration, arterial blood gases, pH, and hematocrit were determined at 0, 1, 2, 3, 4, 6, 8, 10, 12, 16, 20, 24, and 36 hours after initiating the hemorrhage. Plasma EPO concentration was determined by radioimmunoassay. Statistical analyses included analysis of variance and least squares regression. RESULTS: Mean plasma EPO concentration increased significantly (2.3 +/- 0.5/+/- standard error of the mean/times basal values) at 4 hours after initiating the hemorrhage, reached a maximum (33.8 +/- 12.9 times basal values) at 10-16 hours, and decreased to 50% of maximal values at 24 hours after hemorrhage. Hematocrit decreased rapidly during the 12 hours after hemorrhage (P < .0001), whereas arterial blood oxygen tension remained unchanged. The logarithm of EPO concentration was correlated with hematocrit (r = -0.74, P < .0001) but not with arterial blood oxygen tension. CONCLUSIONS: Blood EPO concentration began to increase at approximately 3-4 hours after the onset of hemorrhage. Further, EPO concentrations at 24 hours after hemorrhage represented only 50% of the maximal EPO response. The correlation between EPO and hematocrit suggests that blood oxygen content rather than oxygen tension is the more important stimulant for augmented EPO production after fetal hemorrhage. 相似文献