载脂蛋白E基因多态性与冠心病关系的研究

目的：探讨载脂蛋白Ｅ（ａｐｏＥ）基因多态性在冠心病（ＣＨＤ）发生发展中的作用及其对血脂质、脂蛋白水平的影响。方法：应用聚合酶链反应技术和遗传学方法,测定９３ 例ＣＨＤ患者和９４例正常对照者的ａｐｏＥ基因型;按常规方法测定血浆脂质、脂蛋白水平。结果：共发现５ 种ａｐｏＥ基因型,分别为Ｅ３／３、Ｅ３／２、Ｅ４／３、Ｅ４／２和Ｅ４／４。ＣＨＤ组ａｐｏＥ４／３ 基因型和ε４ 等位基因频率及总胆固醇（ＴＣ）、低密度脂蛋白胆固醇（ＬＤＬ－Ｃ）均显著高于对照组（均Ｐ＜ ０．０１）,ａｐｏＡＩ的水平高于对照组（Ｐ＜ ０．０５）,其他血脂指标无显著性差异（ Ｐ＞ ０．０５）。在ＣＨＤ组各亚型之间,ＴＣ、ＬＤＬ－Ｃ和ａｐｏＡＩ水平之间存在显著性差异（Ｐ＜ ０．０５）。结论：ａｐｏＥε４ 等位基因是ＣＨＤ重要的遗传易患因素,ａｐｏＥ基因多态性亦影响血ＴＣ、ＬＤＬ－Ｃ和ａｐｏＡＩ水平。     

冠心病家族史少年儿童血清脂蛋白（a）的研究

对５４例有冠心病（ＣＨＤ）家族史和１０７例无ＣＨＤ家族史（作对照组）少年儿童检测血清脂蛋白（ａ）［Ｌｐ（ａ）］、载脂蛋白Ａ－Ｉ（ａｐｏＡ－Ｉ）、载脂蛋白Ｂ（ａｐｏＢ）水平及身高、体重五项指标。结果显示：（１）有ＣＨＤ家族史组Ｌｐ（ａ）平均值为１９８．６ｍｇ／Ｌ，对照组则为１３６．０３ｍｇ／Ｌ，两组有差异显著性（Ｐ＜０．０１）；（２）有ＣＨＤ家族史组Ｌｐ（ａ）增高的频率也明显高于对照组；（３）Ｌｐ（ａ）水平与体重无显著相关，与ａｐｏＡ－Ｉ、ａｐｏＢ、年龄和身高也均无相关性。     

力平脂治疗高脂血症的临床观察

报告对１５３例高脂血症患者以力平脂治疗１００例，潘特生治疗５３例。于服力平脂４和８周后分别检测有关指标，总胆固醇（ＴＣ）分别下降２０％、２３％；甘油三酯（ＴＧ）下降５５％、５９％；低密度脂蛋白胆固醇（ＬＤＬ－Ｃ）下降２０％、２３％；高密度脂蛋白胆固醇（ＨＤＬＣ）升高２８％、３８％；ＴＣＨＤＬ－Ｃ／ＨＤＬ－Ｃ比值下降３１％。前后对比差异非常显著（Ｐ＜０．０１～０．００１）。其中１５例重度高ＴＧ血症（ＴＧ≥５．５ｍｍｏｌ／Ｌ），服力平脂８周后，ＴＧ值下降７．２±３．３ｍｍｏｌ／Ｌ下降率为７４．０％，显示其降ＴＧ尤为显著。服药前ＴＧ值越高，治疗后下降越显著。力平脂还可使载脂蛋白Ａ（ａｐｏ－Ａ１）升高，为１５％（４周）、１８％（８周）；ａｐｏ－Ａ１／ａｐｏ－Ｂ比值分别上升４６％、４８％；ａｐｏ－８分别下降１５％、１７％。力平脂组部分病例的ＡＬＴ轻度升高，但为可逆性，其余尚未发现明显副作用。     

90例85岁以上老人血脂,脂蛋白和载脂蛋白的研究

对９０例８５岁以上高龄老人的血脂、脂蛋白和载脂蛋白进行测定，并与陈旧性心肌梗塞（ＯＭＩ）组对比。结果显示：与ＯＭＩ组比较，高龄老人血清ＴＣ、ＬＤＬ－Ｃ、ＡｐｏＡⅠ和ＡｐｏＢ_（１００）的明显低，而ＨＤＬ－Ｃ／ＴＣ、ＨＤＬ－Ｃ／ＬＤＬ－Ｃ和ＡｐｏＡⅠ／ＡｐｏＢ_（１００）明显高；ＴＧ、ＨＤＬ－Ｃ和ＶＬＤＬ－Ｃ在两组之间无统计学差异。单因素相关分析显示，ＨＤＬ－Ｃ／ＴＣ，ＨＤＬ－Ｃ／ＬＤＬ－Ｃ和ＡｐｏＡⅠ／ＡｐｏＢ_（１００）与长寿呈正相关，与ＯＭＩ呈负相关；而ＴＣ、ＬＤＬ－Ｃ、ＡｐｏＡⅠ和ＡｐｏＢ_（１００）则与长寿呈负相关，与ＯＭＩ呈正相关；ＨＤＬ－Ｃ、ＴＧ和ＶＬＤＬ－Ｃ与长寿和ＯＭＩ几乎无相关关系。对各检测指标进行多元逐步回归分析，ＡｐｏＢ_（１００）对长寿作用最大，其次为ＡｐｏＡⅠ／ＡｐｏＢ_（１００）和ＨＤＬ－Ｃ．因此认为血清ＡＰｏＢ_（１００）水平的低值、ＨＤＬ－Ｃ和ＡｐｏＡⅠ／ＡｐｏＢ_（１００）的高值，是高龄老人血脂、脂蛋白和载脂蛋白的特点，亦是长寿的指标。     

冠心病患者血浆氧化修饰低密度脂蛋白水平的变化

对８６例冠心病（ＣＨＤ）患者及６０例正常对照组血浆氧化修饰低密度脂蛋白（ＯＸ－ＬＤＬ）及血脂、脂蛋白和载脂蛋白（ａｐｏ）水平进行了测定。结果显示ＣＨＤ患者血浆ＯＸ－ＬＤＬ、血清甘油三酯（ＴＧ）、低密度脂蛋白（ＬＤＬ）及ａｐｏＢ１００均显著高于正常对照组，而血清高密度脂蛋白（ＨＤＬ）、ａｐｏＡＩ及ａｐｏＡＩ／ａｐｏＢ１００均显著低于正常对照组；相关分析表明，血浆ＯＸ－ＬＤＬ水平与ＴＧ、ＬＤＬ、ａｐｏＢ１００水平均呈显著正相关，而与ＨＤＬ、ａｐｏＡＩ及ａｐｏＡＩ／ａｐｏＢ１００水平均呈显著负相关；多元逐步回归分析表明ＯＸ－ＬＤＬ对ＣＨＤ的预测价值优于目前临床常用的血脂、脂蛋白及ａｐｏ。认为，ＯＸ－ＬＤＬ与ＣＨＤ的发生发展密切相关，ＣＨＤ患者体内ＬＤＬ氧化修饰增加，在治疗过程中应加强抗氧化治疗以抑制ＬＤＬ在体内的氧化修饰。     

二甲基硫脲对心肌细胞抗过氧化氢损伤的实验研究

为探讨二甲基硫脲（ＤＭＴＵ）保护心肌细胞抗过氧化氢（Ｈ２Ｏ２）损伤。３２瓶培养乳鼠心肌细胞随机分４组，每组８瓶：（１）对照组；（２）Ｈ２Ｏ２（５ｍｍｏｌ）组；（３）ＤＭＴＵ（２０ｍｍｏｌ）组；（４）Ｈ２Ｏ２（５ｍｍｏｌ）＋ＤＭＴＵ（２０ｍｍｏｌ）组。在３７℃、５％ＣＯ２的ＭＥＭ培养４小时。结果发现：（１）与对照组比，Ｈ２Ｏ２组乳酸脱氢酶（ＬＤＨ，Ｕ／１００ｍｌ）释放多（２８２．３８±４７．２８比７７．２５±１８．２５，Ｐ＜０．０１）、ＴＢＡ反应物（ＴＢＡＲＳ，ｎｍｏｌ／ｍｇＰｒ．）产生多（２．２５±０．５３比０．７９±０．３６，Ｐ＜０．０１）；（２）与Ｈ２Ｏ２组比，Ｈ２Ｏ２＋ＤＭ－ＴＵ组ＬＤＨ释放少（９９．２５±４１．８８比２８２．３８±４７．２８，Ｐ＜０．０１）；ＴＢＡＲＳ产生少（０．５９±０．１８比２．２５±０．５３，Ｐ＜０．０１）；此外，我们还发现ＤＭＴＵ组超氧化物歧化酶（ＳＯＤ，μｇ／ｍｇＰｒ．）比对照组高（８．４９±３．６５比１．９２±１．４０，Ｐ＜０．０１）。ＤＭＴＵ能保护心肌细胞抗Ｈ２Ｏ２损伤，机制与灭活羟自由基（·ＯＨ）、保护ＳＯＤ活性有关     

褐藻胶对大鼠实验性肝纤维化的防治作用

目的研究褐藻胶对大鼠实验性肝纤维化的防治作用．方法用４０％四氯化碳（ＣＣｌ４）制备大鼠肝纤维化模型，实验分组为正常对照组（ｎ＝８），ＣＣｌ４组（ｎ＝８），秋水仙硷（ＣＯＬ）组（ｎ＝６）和褐藻胶组（ｎ＝６）（２００ｍｇ／ｋｇ，ｉｇ，３次／周×４）．观察肝脏组织学和血清Ⅲ型前胶原（ＰＣⅢ）及透明质酸（ＨＡ）水平的变化．结果褐藻胶组ＰＣⅢ（１４２８μｇ／Ｌ±７６１μｇ／Ｌ）和ＨＡ水平（２６５５μｇ／Ｌ±９３１μｇ／Ｌ）显著低于ＣＣｌ４组（２９３５μｇ／Ｌ±７８３μｇ／Ｌ，５１９８μｇ／Ｌ±１１８３μｇ／Ｌ，Ｐ＜００１），而褐藻胶组与ＣＯＬ组间无显著差异．病理学观察显示，ＣＣｌ４组肝纤维化程度重于褐藻胶组和ＣＯＬ组．结论褐藻胶对实验性肝纤维化有防治作用．     

老年男性冠心病患者血清硫酸脱氢表雄酮含量变化的临床意义

目的观察老年男性冠心病患者血清硫酸脱氢表雄酮（ＤＨＥＡ－Ｓ）含量变化，探讨其与睾酮（Ｔ）、胰岛素（ＩＮＳ）、血糖（Ｇｌｕ）、甘油三酯（ＴＧ）、总胆固醇（ＴＣ）、载脂蛋白Ｂ（ａｐｏＢ）及年龄的相关性。方法用放免法测定６９例老年男性冠心病患者血清ＤＨＥＡ－Ｓ含量，并与３５例年龄匹配的男性健康人对照。结果冠心病组ＤＨＥＡ－Ｓ含量（２．９６±１．８０μｍｏｌ／Ｌ）显著低于对照组（４．０６±１．７６μｍｏｌ／Ｌ，Ｐ＜０．０１），病情重组（２．４４±１．３６μｍｏｌ／Ｌ）又明显低于病情轻组（３．３２±２．１２μｍｏｌ／Ｌ，Ｐ＜０．０５）；冠心病组ＤＨＥＡ－Ｓ含量与年龄呈负相关（ｒ＝－０．３０５４，Ｐ＜０．０１），对照组两者也呈负相关（ｒ＝－０．３６１５，Ｐ＜０．０５）；冠心病患者ＤＨＥＡ－Ｓ降低与空腹血清ＩＮＳ、ＴＧ及ａｐｏＢ增高均呈负相关（分别为ｒ＝－０．３２９７、－０．２５１９及－０．２４１３，Ｐ＜０．０１或０．０５）。结论老年男性冠心病患者血清ＤＨＥＡ－Ｓ含量降低，并与冠心病某些危险因素如老年、高胰岛素血症、高甘油三酯血症、血清ａｐｏＢ高值相关，但其确切的发病机理有待深入研究。     

克拉玛依市特殊人群碘缺乏病监测结果分析

采用放射免疫法对克拉玛依市区５１０例新生儿脐带血ＴＳＨ和１２２例２～４岁儿童的ＴＳＨ及１２７例８～１０岁儿童尿碘进行测定,市区新生儿ＴＳＨ值〉５μＩＵ／ｍｌ 占２２．９％;２～４岁儿童ＴＳＨ值大〉５μＩＵ／ｍｌ占１．７％,８～１０岁儿童尿碘〉１００μｇ／Ｌ占９８．４％,〈１００μｇ／Ｌ 占１．６％。甲状腺肿大率为１６．６７％（触诊法）。调查结果表明克拉玛依市还存在着碘缺乏。     

磺脲类降糖药对HIT细胞胰岛素分泌刺激作用的分子机制研究

通过对ＨＩＴ细胞的体外试验，探讨了甲磺丁脲及优降糖抑制ＡＴＰ敏感性钾离子通道和钾的外流、促进细胞外钙离子的内入和胰岛素释放的一系列分子生物学机制，并且对此两种药物的作用强度及特点进行了比较。二者与β细胞受体的亲和常数分别为０．４±０．１ｎｍｏｌ／Ｌ和２．５±０．５μｍｏｌ／Ｌ；抑制８６Ｒｂ＋外流的ＩＣ５０分别为０．７±＋０．５ｎｍｏｌ／Ｌ和１．９±０．８μｍｏｌ／Ｌ；促进细胞内钙离子升高的ＥＤ５０分别为０．９ｎｍｏｌ／Ｌ和２．４μｍｏｌ／Ｌ，刺激胰岛素分泌的ＥＤ５０则分别为１．０±０．４ｎｍｏｌ／Ｌ和４．３±２．２μｍｏｌ／Ｌ。ＡＴＰ－敏感性钾离子通道激动剂──二氮嗪（０．４ｍｍｏｌ／Ｌ）能在上述各个环节阻断优降糖对β细胞的作用；而钙离子通道抑制剂──尼莫地平（１μｍｏｌ／Ｌ）则能在不影响８６Ｒｂ＋外流的情况下显著减低细胞内钙离子的浓度，进而抑制ＨＩＴ细胞的胰岛素分泌。表明此类药物能在不影响腺苷酸环化酶─ｃＡＭＰ系统的情况下，通过促进胰岛β细胞膜上ＡＴＰ敏感性钾离子通道的关闭，导致电压依赖性钙离子通道的开启，进而触发了细胞外钙离子的内入和胰岛素的分泌。     

Autoantibodies to oxidized low density lipoprotein: the relationship to low density lipoprotein fatty acid composition in diabetes

Autoantibodies to oxidized low density lipoprotein have been shown to be an independent predictor of the progression of carotid atherosclerosis. This study examines the relationship between low density lipoprotein fatty acid composition and autoantibodies to both malondialdehyde-modified and copper-oxidized low density lipoprotein in non-diabetic patients with (n = 17), and without (n = 18), definite evidence of previous myocardial infarction. The third group were non-insulin-dependent diabetic patients with no evidence of atherosclerosis (n = 15) and the fourth group were patients with non-insulin-dependent diabetes (n = 17) who had definite evidence of previous myocardial infarction. Fatty acids were measured by gas-liquid chromatography. Antibodies to malondialdehyde-modified low density lipoprotein and copper-oxidized low density lipoprotein were determined by an ELISA method. Autoantibodies to copper-oxidized low density lipoprotein were significantly higher in the non-diabetic patients with heart disease when compared to any other group (p < 0.05). Autoantibodies to malondialdehyde-modified low density lipoprotein were significantly higher in the non-diabetic subjects with heart disease and in both diabetic groups compared to non-diabetic subjects without coronary heart disease (p < 0.05). Lineolic acid (%) in low density lipoprotein did not differ between groups but arachidonic acid (%) was significantly lower in both diabetic and non-diabetic patients with coronary heart disease (p < 0.05). The diabetic patients with low antibodies had 39.6 ± 2.2 % polyunsaturated fatty acids in their low density lipoprotein while diabetic patients with high antibodies had 46.7 ± 1.2 % polyunsaturates in their low density lipoprotein (p < 0.01). This study confirms the association between antibodies to oxidized low density lipoprotein and coronary heart disease and shows raised low density lipoprotein antibody levels in diabetic patients with and without demonstrable atherosclerosis. In the diabetic patients, those with high antibody levels had high polyunsaturated fatty acid levels in their LDL suggesting a possible role for dietary intervention. © 1997 John Wiley & Sons, Ltd.     

低密度脂蛋白对胰岛细胞功能的影响

目的探讨低密度脂蛋白（LDL）受体在胰岛β细胞（NIT-1细胞）上的表达及其LDL和氧化型LDL对胰岛β细胞功能的影响。方法实验室培养NIT-1细胞,用RT-PCR扩增后电泳检测LDL受体在NIT-1细胞上的表达;检测LDL及氧化型LDL对胰岛β细胞活性和胰岛素分泌及胰岛素mRNA表达的影响。结果LDL受体在NIT-1细胞上表达;与对照组相比,LDL及氧化型LDL对胰岛β细胞的活性无明显影响（P〉0．05）;LDL对胰岛β细胞胰岛素分泌无明显影响（P〉0．05）,氧化型LDL能显著抑制胰岛β细胞胰岛素的分泌（P〈0．01）;LDL对胰岛β细胞胰岛素mRNA表达无明显影响（P〉0．05）,氧化型LDL能显著抑制胰岛β细胞胰岛素mRNA表达（P〈0．01）。结论LDL受体在NIT-1细胞表达;LDL及氧化型LDL对胰岛β细胞活性无明显影响,但氧化型LDL显著降低胰岛β细胞胰岛素的分泌和细胞胰岛素mRNA的表达。     

Effects of nicotinic acid on serum cholesterol concentrations of high density lipoprotein subfractions HDL2 and HDL3 in hyperlipoproteinaemia

Nicotinic acid was given in a 4-g daily dose for 6 weeks to 41 weight-stable patients of mean age (+/- SD) 52 +/- 9 years, with type IIa, type IIb or type IV hyperlipoproteinaemia (HLP), in order to study its effects on serum cholesterol concentrations of high density lipoprotein (HDL) subfractions HDL2 and HDL3. The triglyceride and cholesterol levels of serum very low density (VLDL) and low density (LDL) lipoproteins decreased during treatment (P less than 0.001). Serum HDL and HDL2 cholesterol levels increased by 37% and 135%, respectively. These changes were positively correlated (r = 0.93; P less than 0.001). There was no significant change in mean serum HDL3 cholesterol concentration. A negative correlation existed between changes in HDL3 and HDL2 cholesterol levels (r = -0.54; P less than 0.001). Multiple stepwise linear regression analyses revealed that the initial HDL3 cholesterol predicted more than 30% of the increase in HDL2 cholesterol. Changes in the concentrations of HDL2 and HDL3 cholesterol after 6 weeks of drug treatment were not related to the type of HLP, neither were these effects of nicotinic acid correlated with changes in VLDL or LDL lipid levels. The concept has previously been proposed, on the basis of in vitro data, that HDL2 is formed from HDL3 particles in the blood. Our results suggest that, in man, this reaction is stimulated in vivo by prolonged nicotinic acid therapy.     

Soluble low-density lipoprotein receptor-related protein

Soluble forms of receptors can influence the activity of their membrane-bound counterparts by affecting their interactions with ligands. Low density lipoprotein (LDL) receptor-related protein (LRP), a member of the LDL receptor family, binds multiple classes of ligands and has been implicated in a broad range of normal and disease processes involving lipid metabolism, protease clearance, and cell migration. We recently identified a soluble form of LRP (sLRP) in human plasma and showed that it retains LRP-ligand binding ability. These findings open potentially important additional aspects in the biology of this multifunctional receptor. This review summarizes characteristics of soluble LRP and relates these to the membrane-bound form of the receptor.     

Cholesteryl ester transfer activity : Localization and role in distribution of cholesteryl ester among lipoproteins in man

The cholesteryl ester exchange/transfer protein is involved in the transport of cholesteryl ester from high density lipoproteins (HDL) to very low density lipoproteins (VLDL) and low density lipoproteins (LDL). Localization of cholesteryl ester transfer activity (CETA) in plasma was studied by measuring CETA in various delipidated fractions from a single step density ultracentrifugation gradient of plasma. CETA was measured in an in vitro system by calculating the exchange of cholesteryl ester in a standard mixture of [3H]CE-HDL and LDL. The method used for the delipidation of plasmas and fractions to be tested was critical. Optimal results were obtained by delipidation with diisopropylether-butanol (60: 40, v/v) at O degrees C. The bulk of CETA was detected in HDL3 (1.125 less than d less than 1.210 g/ml) when the lipoproteins were separated by single-step density gradient ultracentrifugation and in the 'lipoprotein-free' fraction (d greater than 1.250 g/ml) when the lipoproteins were separated by flotation ultracentrifugation including two washes. To determine whether CETA plays a role in the distribution of cholesteryl ester among the various lipoproteins, it was measured in whole plasma from normal and hyperlipidemic subjects. Plasma was delipidated before the assay in order to prevent bias due to variation of cholesterol content. CETA was higher in delipidated plasma of hyperlipidemic subjects (117.3 +/- 36.5 nmol CE/ml/h) than in delipidated plasma of normolipidemic controls (68.7 +/- 17.6 nmol CE/ml/h) (P less than 0.005). A positive correlation (r = 0.80, P less than 0.005) was found between CETA and (VLDL + LDL) cholesterol levels. A negative correlation (r = 0.57, P less than 0.05) existed between CETA and HDL cholesterol. This correlation was found both in the group as a whole and within the normal and the hyperlipidemic groups separately. The activity of the cholesteryl ester transfer appears to be a regulatory factor in the distribution of cholesteryl ester over the various lipoproteins.     

冠心病患者及高危人群血脂异常分析

目的 :观察甘肃地区冠心病 (CHD)和高危人群的低密度脂蛋白胆固醇 (LDL C)的异常情况。方法 :分析 2 2 0例冠状动脉造影 (CAG)确诊的CHD者 (CHD组 )及 90例富含CHD危险因素、CAG阴性的非CHD者(NCHD组 )的总胆固醇 (TC) ,三酰甘油 (TG) ,LDL C ,高密度脂蛋白胆固醇 (HDL C)水平及异常率。结果 :与NCHD组相比 ,CHD组的平均TC和LDL C水平明显增高 ,HDL C水平降低 (P <0 .0 5 )。CHD组LDL C和HDL C异常率明显大于NCHD组 ,分别为 6 8.6 % :4 6 .7%和 5 0 % :2 6 .7% (均P <0 .0 1)。CHD组患者LDL C水平的达标率为 33%左右。结论 :LDL C水平升高和HDL C水平降低是该地区CHD发生和发展的关键。CHD及其高危人群中他汀类药物的应用应加强并且有很大空间。通过药物开发提高HDL C水平在防治CHD事件将有很好的前景     

不同类型冠心病患者血浆氧化低密度脂蛋白及其抗体变化的临床意义

目的　探讨急性冠脉综合征 (ACS)、稳定型心绞痛 (SAP)患者血浆血脂、氧化低密度脂蛋白 (OX-L DL)及其抗体 (Anti- OX- L DL)变化在冠状动脉粥样硬化 (AS)发生、发展中的作用及其相关性。方法　分别用酶联免疫吸附法测定 136例冠心病 (ACS组 10 6例 ,SAP30例 )及 5 6例正常对照组的血浆血脂、OX- L DL、Anti- OX-L DL,并进行直线相关性分析。结果　 1冠心病组血脂 TC、TG、L DL- C与对照组存在显著差异 (P<0 .0 1)。 2ACS组血浆 OX- L DL及 Anti- OX- L DL与 SAP组、对照组比较 ,P均 <0 .0 1;SAP组与对照组比较上述指标差异无显著性 (P>0 .0 5 )。 3ACS组 OX- L DL与 L DL呈正相关 (r=0 .6 4 2 ,P<0 .0 1) ;Anti- OX- L DL与 L DL无关 (r=0 .341,P>0 .0 5 )。结论　高脂血症是冠心病的绝对危险因素 ;OX- L DL及其抗体在 ACS发病中起重要作用 ;Anti- OX- L DL 作用不依赖于 L DL。     

葡萄糖对巨噬细胞不同脂蛋白受体表达的影响

目的研究不同浓度葡萄糖对巨噬细胞高密度脂蛋白受体(SR-BI)及凝集素样氧化型低密度脂蛋白受体(LOX-1)表达的影响。方法2003年5月至2005年1月在中国医科大学附属第一医院将5.6mmol/L、11.1mmol/L、16.7mmol/L和33.3mmol/L葡萄糖与诱导分化48h后的U937细胞共同孵育24h及16.7mmol/L葡萄糖与诱导分化48h后的U937细胞作用0,12,24,48,72h后,用Western印迹法检测SR-BI及LOX-1蛋白的表达。结果葡萄糖对巨噬细胞SR-BI蛋白表达量无显著影响(P>0.05)。葡萄糖浓度升高及作用时间延长可使LOX-1蛋白表达增加。结论葡萄糖诱导LOX-1蛋白表达增加并呈浓度和时间依赖性。     

高密度脂蛋白对氧化型低密度脂蛋白抑制的血管平滑肌细胞一氧化氮释放的影响

为观察高密度脂蛋白是否能损坏抗氧化型低密度脂蛋白对血管平滑肌细胞释放一氧化氮的抑制作用，培养了人脐动脉平滑肌细胞并用脂多和γ－博古纱诱导一氧化氮的释放。应用ＧＲＩＥＳＳ试剂测定细胞培养基中亚硝酸盐的含量，以免疫组织化学染色和逆转录－聚合酶链反应分析导型一氧化氮合酶基因的表达。结果发现，氧化型低密度脂蛋白可使脂多糖和γ－干扰素诱导的平滑肌细胞一氧化氮合酶及其ｍＲＮＡ水平下降，抑制一氧化氮释放。而高密     

Alteration of platelet counts and lipid profiles after treatment of acute Plasmodium vivax

During malaria infections, thrombocytopenia and low cholesterol levels are frequently observed changes. We compared these changes in patients admitted with fevers and infected with Plasmodium vivax, patients admitted with fevers with respiratory/urinary infections and afebrile normal (control) non-infected volunteers. Changes in the platelet count and lipid parameters are reported for malaria patients after treatment with hydroxychloroquine and primaquine for acute P. vivax malaria. Of a total 141 participants, 55 patients were diagnosed with malaria (positive blood smear) prior to treatment. Compared to the normal (n=52) and non-malaria fever groups (n=34), there was a significant decrease in five hematologic indices (white blood cell, red blood cell, hemoglobin, hematocrit and platelet) and three lipid parameters (total cholesterol, HDL-c and LDL-c) in the vivax malaria group at day 0 (pre-treatment). Following treatment, the platelet counts returned to normal limits (P<0.05) from 91,058/microL on day 0 to 246,833/microL by day 17 after treatment. However, changes in the lipid parameters of malaria patients showed a slow recovery to normal limits compared to the platelet counts. The HDL-c and LDL-c remained low for 1 month after treatment but increased at 3 and 6 months post-treatment. At 12 months after treatment, the levels of two lipid parameters had fully recovered to the normal limits. Thus, special attention should be applied when interpreting laboratory blood profiles of malaria patients, especially platelet and lipid based tests, until full recovery after treatment.