急性白血病病人ECR1基因多态性与红细胞免疫功能的关系

目的:探讨急性白血病病人红细胞补体受体Ⅰ型分子(ECR1)基因密度多态性与红细胞免疫黏附功能的相关性。方法:急性白血病46例,正常对照50例,采用PCR-RFLP法测定RBC-CR1基因型。以红细胞黏附花环试验测定RBC-C3b受体花环率(RBC-C3bRR)及免疫复合物花环率(RBC-C ICR)。结果:①与对照组比,急性白血病病人RBC-C3bRR下降,RBC-C ICR上升(P<0.01);②急性白血病病人HH型、HL型LL型分别是60.87%、30.44%、8.69%,突变率是39.13%,与对照组相比无统计学意义(P>0.05);③在急性白血病组内,HL型的RBC-C3bRR为17.23±5.74,低于HH型的26.06±6.21;RBC-C ICR为7.36±2.45,高于HH型的4.63±2.00,其差异有统计学意义(P<0.01)。结论:急性白血病病人RBC免疫功能低下,ECR1基因缺陷对病人的RBC免疫能力下降有一定影响。不同ECR1基因型的人群红细胞免疫功能有差异,HH型的红细胞免疫黏附功能强于HL型。     

肿瘤患者红细胞免疫功能与SAPO-1/Fas、TNF-a、NO表达水平的实验研究

目的:为了探讨肿瘤患者红细胞免疫功能与SAPO-1/Fas、TNF-a、NO表达水平的相关性及相互作用。方法:采用酵母菌-红细胞免疫粘附试验,对45例化疗前和17例化疗后病情缓解者进行了红细胞C3b受体花环试验(RBC-C3bRR)和红细胞免疫复合物花环试验(RBC-ICR)及ELISA法对107例肿瘤患者及22例健康人进行了SAPO-1/Fas的检测,其中部分进行了TNF-a、NO检测。结果:肿瘤患者血清SAPO-1/Fas表达水平明显高于健康人对照组(P<0.001),且与肿瘤种类无关;17例化疗后病情缓解者SAPO-1/Fas表达水平与化疗前显著降低(P<0.05)。45例和47例肿瘤患者血清同时检测SAPO-1/Fas、TNF-a表达水平及SAPO-1/Fas、NO含量并进行单因素直线相关分析,分别为r=0.76,P<0.05,r=0.72,P<0.05,呈显著正相关。RBC-C3bRR明显降低,45例肿瘤患者化疗前又明显低于17例病情缓解者(P<0.01),RBC-ICR明显高于健康人,45例化疗前肿瘤患者RBC-ICR明显高于17例化疗后病情缓解者(P<0.01)。结论:肿瘤患者RBC-ICR和SAPO-1/Fas、TNF-a、NO表达水平明显增高,呈显著正相关,RBC-C3bRR明显降低。为研究红细胞免疫与淋巴细胞免疫的相互关系及肿瘤治疗提供了新的理论基础和实验依据。     

Ⅱ型糖尿病病人红细胞免疫功能的研究

通过测定30例糖尿病病人的红细胞免疫功能表明,糖尿病病人的红细胞C_3b受体花环率(RBC-C_3bRR)降低,红细胞免疫复合物花环率(RBC-ICR)及循环免疫复合物(CIC)增高;且CIC与RBC-ICR呈正相关,与RBC-C_3bRR呈负相关.RBC-ICR与RBC-C_2bRR呈负相关.提示糖尿病病人存在红细胞免疫功能低下,可能为继发性改变.     

血小板活化因子对40例新生儿缺氧缺血性脑病脑损伤作用机制的研究

目的:研究新生儿缺氧缺血性脑病(HIE)血清血小板活化因子(PAF)水平的变化,探讨PAF在HIE脑损伤中的作用及其作用机制。方法:采用生物学方法测定新生儿血清PAF水平,并观察其与脑损伤、脑血流量和1minApgar评分之间的关系。结果:①中、重度HIE患儿急性期血清PAF为(117.7±19.28)μg/L,显著高于正常对照组犤(41.92±14.66)μg/L,P<0.001犦及轻度HIE患儿犤(48.96±15.09)μg/L,P<0.01犦,恢复期犤(45.34±15.98)μg/L,P>0.05犦降至正常水平;轻度HIE患儿血清PAF水平与正常对照组相比差异无显著性。②伴重度脑损伤的HIE患儿血清PAF水平显著高于轻度脑损伤的HIE患儿犤(133.16±19.13)μg/L比(101.13±8.59)μg/L,P<0.01犦。③伴脑血流量异常的HIE患儿血清PAF水平较不伴脑血流量异常的HIE患儿明显升高犤(125.43±24.80)μg/L比(105.41±15.48)μg/L,P<0.01)犦。④HIE患儿血清PAF水平与1minApgar评分呈负相关。结论:PAF在新生儿缺氧缺血性脑损伤的发病机理中起重要作用,其介导脑损伤的发生可能是通过下调脑血流量来实现的。     

Correlation between Erythrocyte Immune Function and TCM Syndromes of COPD Patients at Stable Stage

目的 研究慢性阻塞性肺疾病稳定期中医证型与红细胞免疫功能的相关性.方法 按《慢性阻塞性肺疾病(COPD)诊治指南(2007年修订版)》和中医辨证标准收治肺气虚、脾气虚、肾气虚组COPD稳定期的患者和正常对照组各30例,分析4组的红细胞免疫(RBC-C3bRR、RBC-I-CR)差异.结果 COPD组RBC-C3bRR和RBC-ICR与正常对照组比较,差异具有显著的统计学意义;在肺气虚、脾气虚、肾气虚3个证型中,差异也有显著的统计学意义,肺气虚组→脾气虚组→肾气虚组RBC-C3bRR逐步减少,RBC-ICR逐步增高.结论 RBC-C3bRR、RBC-ICR可作为COPD稳定期患者辨证分型、病情预后、疗效判定的参考指标.     

针药复合麻醉对体外循环心脏手术患者红细胞免疫功能的影响

选择二尖瓣置换术患者40例,随机分为针刺辅助全麻组(A组)、全麻组(B组),测定红细胞C3b受体花环率(RBC-C3bRR)、红细胞免疫复合物花环率(RBC-ICR)、丙二醛(MDA)、超氧化物歧化酶(SOD)。两组RBC-C3bRR及SOD于CPB结束开始回升,到术后24 h仍未达到术前水平,但A组高于B组(P<0.01);两组RBC-ICR及MDA于CPB结束后开始下降,A组低于B组(P<0.01),到术后24 h A组接近术前水平。针刺辅助全麻对体外循环下心脏手术患者红细胞免疫功能有一定的保护作用。     

偏头痛发作期间的QTc离散度和P波离散度

本研究旨在探讨偏头痛发作期间的QTc离散度和P波离散度的增加情况。本研究共纳入55例(年龄16～65岁,女性49例,男性6例)偏头痛患者。在偏头痛发作期间和头痛间歇期,采用12导联ECG分别测量患者的心率、QTc间期、最大和最小QTc间期、QTc离散度、最大和最小P波持续时间以及P波离散度。通过扫描仪将ECG转换至个人电脑,然后使用Adobe Photoshop软件放大400倍。与头痛间歇期相比,偏头痛发作期的最大QTc间期犤(454±24)m s vs(429±23)m s,P<0.001犦、QTc间期犤(443±26)m s vs(408±22)m s,P<0.001)犦以及QTc离散度犤(63±18)m s vs(43…     

消化性溃疡患者红细胞免疫功能的观察

目的:探讨幽门螺杆菌(Hp)感染对消化性溃疡患者红细胞免疫功能的影响。方法:检测试验组120例消化性溃疡患者与对照组60例健康体检者红细胞免疫功能情况,并进行比较。结果:试验组RBC-C3bRR、RFER明显低于对照组(P<0.01,P<0.05),RBC-ICR、RFIR高于对照组(P<0.05,P<0.01);Hp阳性组RBC-C3bRR明显低于Hp阴性组(P<0.05),RBC-ECR明显高于Hp阴性组(P<0.05),RFER、RFIF两组间比较,差异无统计学意义(P>0.05);Hp阳性患者根除治疗前、后红细胞免疫功能对比发现治疗后RBC-ICR明显低于根除前。结论:Hp的感染可能是消化性溃疡患者红细胞免疫功能下降的主要原因。     

结肠癌患者红细胞免疫功能检测及其临床意义

目的为了解结肠癌患者红细胞免疫功能的变化及其临床意义.方法对50例结肠癌病人和20例健康人进行红细胞免疫功能检测并作统计学处理.结果结肠癌患者术前红细胞C3b受体花环率(RBC-C3bRR)为10.8±3.60%,术后为19.5±4.60%,对照组为20.80±5.00%,患者术前与术后及对照组比较有显著性差异(P＜0.01)结肠癌患者术前红细胞免疫复合物花环率(RBC-ICR)为18.60±5.00%,术后为15.90±5.5%,对照组为10.50±3.00%,对照组与患者组有显著性差异(P＜0.01);结肠癌组循环免疫复合物含量(CIC)术前为18.60±4.10%,术后为8.0±3.00%,对照组为5.2±2.00%,患者组术前与术后、对照组有显著性差异,(P＜0.01).结论红细胞免疫功能的变化可作为结肠癌患者的辅助诊断、预后监测以及疗效观察的一项免疫学参考指标.     

电针对中老年带状疱疹患者细胞免疫功能及早期血液流变学的影响

目的:探讨电针对中老年带状疱疹患者细胞免疫功能与早期血液流变学的影响。方法:选择中老年带状疱疹患者100例,随机分为治疗组、对照组各50例,采用双抗体夹心ELISA法检测血清可溶性白细胞介素-2受体(sIL-2R);流式细胞仪测定外周血T淋巴细胞亚群(CD3+,CD4+,CD8+)及红细胞C3b受体花环(RBC-C3bRR)和红细胞免疫复合物花环(RBC-ICR);同时检测血液流变学。结果:与对照组比较,治疗组sIL-2R明显降低(P<0.01),T淋巴细胞亚群CD3+,CD4+,CD8+明显升高(P<0.01),CD4+/CD8+升高(P<0.05),RBC-C3bRR明显升高(P<0.01),RBC-ICR明显降低(P<0.01),血液流变学检测指标改善(P<0.05,P<0.01)。结论:电针疗法对中老年带状疱疹患者细胞免疫功能及早期血液流变学有一定的改善作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.