MR纳米分子影像探针靶向示踪口腔鳞状细胞癌研究进展

既往对口腔鳞状细胞癌(OSCC)细胞在体内迁移能力进行实验分析时,多采用侵袭性方法,即在细胞移植后一定时间内处死实验动物,对其口腔颌面部组织进行病理切片分析,并不能对同一实验动物OSCC细胞在体内的迁移和增殖情况进行动态观察。近年来,随着非侵袭性检查手段不断进步,通过以纳米颗粒作为对比剂的MR检查技术,可标记OSCC细胞内高度特异性表达的蛋白,从而靶向示踪其在体内的迁移,并由此推测OSCC的具体分期。本文主要对MR纳米分子探针靶向示踪OSCC的研究进展进行综述。     

Src家族酪氨酸激酶Fyn在人食管鳞状细胞癌中的表达

目的 探讨食管鳞状细胞癌组织中Fyn的表达水平及其意义.方法 收集13例食管鳞状细胞癌组织(ESCC)和10例食管正常黏膜上皮组织(UNR),采用免疫组织化学法和免疫印迹法分析食管鳞状细胞癌组织及食管正常黏膜组织Fyn蛋白表达水平的变化.结果 免疫组织化学染色显示食管鳞状细胞癌组织中Fyn蛋白表达水平(9.68±2.31)高于食管正常黏膜组织中染色指数(3.21±1.25),差异有统计学意义(P<0.01).免疫印迹结果亦显示食管鳞癌组织中Fyn蛋白表达强于食管正常黏膜组织.结论 Fyn蛋白在食管鳞状细胞癌组织中高表达,提示Fyn基因在食管鳞状细胞癌的发生发展过程中可能起重要作用.     

三级淋巴结构在头颈部鳞状细胞癌中的研究进展

头颈部鳞状细胞癌(HNSCC)是全球范围内第六常见的恶性肿瘤。约60%的患者就诊时即为晚期,且发病率存在逐渐上升的趋势,但3年生存率目前仍未得到明显提高。由于确诊时大多患者已到晚期,给治疗带来了较大的困难,并导致了不好的预后产生。近年来多项研究发现三级淋巴结构(TLS)存在于HNSCC患者手术切除标本的肿瘤中及肿瘤周围...     

MCL-1在口腔鳞状细胞癌中的表达及意义

目的:探讨MCL-1在口腔鳞状细胞癌(Oral squamous cells carcinoma,OSCC)组织中的表达情况及意义。方法:采用免疫组织化学和实时荧光定量PCR技术,检测OSCC和口腔正常黏膜组织中MCL-1蛋白及mRNA的表达。结果:MCL-1蛋白在OSCC组织中的阳性表达率为61.9%(52/84),在口腔正常黏膜组织中的阳性表达率为15.0%(3/20),两组有显著性差异(P0.001);MCL-1 mRNA在OSCC肿瘤组织中的含量高于癌旁正常组织(P0.05)。结论:MCL-1的高表达在OSCC的发展过程中可能发挥了重要作用。     

原发性甲状腺鳞状细胞癌1例

患者女,77岁。因发现右侧颈部包块4个月,进行性增大,伴声嘶、呛咳1个月入院。体查:甲状腺Ⅲ度肿大,于右侧甲状腺中下极可及一约3.0cm×5.0cm包块,其质硬、表面欠光滑、无压痛,随吞咽上下活动的肿物,右侧颈部可及多个1.5~3cm肿大的淋巴结。B超检查示甲状腺左叶混合性肿物,以实性为主,右侧甲状腺实性占位性病变,颈部淋巴结大,以右侧明显。甲状腺、全身Ⅰ扫描示:(1)甲状腺右叶“冷节结”,左叶正常显影;(2)颈部淋巴结及全身其它部位未见明显异常浓聚灶。CT扫描示右侧甲状腺占位性病变,考虑肿瘤性病变,并右颈部多个淋巴结肿大。喉镜检查右侧声…     

Tollip参与Src家族酪氨酸激酶Fyn在人食管鳞状细胞癌中负调控机制

目的 探讨人食管鳞状细胞癌中Tollip对Src家族酪氨酸激酶Fyn表达的负调控机制.方法 收集3例新鲜食管鳞状细胞癌组织(ESCC)和食管正常黏膜上皮组织(UNR),采用免疫印迹法分析Tollip蛋白表达水平的变化.同时转染人Tollip质粒DNA人TE1细胞株中观察其对Fyn表达的影响.结果　免疫印迹结果　显示食管鳞癌组织Tollip蛋白表达低于食管正常黏膜组织.转染Tollip质粒DNA可以降低人食管鳞状细胞癌TE1细胞中Fyn的表达.结论 Tollip可能是人食管鳞状细胞癌中Src家族酪氨酸激酶ryn的负调控因子,在食管鳞状细胞癌的发生发展过程中起重要作用.

Abstract：

Objective To determine the relationship between Src family tyrosine kinases Fyn expression and Toll-interacting protein ( Tollip ) in human esophageal squamous cell carcinoma ( ESCC ).Methods The Tollip expression was detected in 3 cases of ESCC and compared to the unremarkable epithelium by Western blotting. Tollip plasmid DNA ( 1 μg) was transfected into TE1 cells and the Fyn/Tollip expression was examined by Western blotting. Results The Tollip expression level was lower in ESCC group than in UNR group. Tollip decreased Fyn protein expression in TE1 cells. Conclusion Tollip may act as a negative regulator of Fyn in human ESCC, and play an important role in human ESCC progress.     

阴茎假血管肉瘤样鳞状细胞癌(附1例报告)

目的 探讨阴茎假血管肉瘤样鳞状细胞癌(PASCC)的诊治方法.方法 回顾分析l例阴茎PASCC癌患者的临床资料,分析其发病情况、临床病理特征、诊断和治疗.结果 首次行阴茎部分切除、双侧腹股沟淋巴结清扫术(T2N2M0),辅以盆腔放疗,术后2个月,因阴茎皮瓣坏死(伴局部复发)再次行阴茎全部切除术.首次术后11个月,因肺部广泛转移死亡.肿瘤组织主要由梭形、多形性的肿瘤细胞和局灶的鳞状细胞癌细胞组成,排列呈血窦状血管样腔隙或网状结构.腹股沟淋巴结仅见普通型高分化鳞状细胞癌细胞(3/9.2/10).复发肿瘤的结构和形态与原发肿瘤相似.免疫组化染色示肿瘤细胞CK(AE1/AE3)、34 β E12、Vimentin(+),EMA呈灶性或片状(+).CD31、CD34、FⅧRAg、HMB45、SMA、Desmin(-).结论 阴茎PASCC是一种罕见的恶性肿瘤,确诊需依赖组织病理学及免疫组织化学检查,早就诊、早诊断和及时恰当的治疗是关键,但预后差.     

口腔鳞状细胞癌中单核细胞趋化蛋白-1表达与肿瘤血管生成的关系

目的在其它肿瘤的研究中发现单核细胞趋化蛋白-1(monocyte chemoauractant protein-1，MCP-1)参与了肿瘤的血管生成，本研究探讨口腔(鳞状细胞癌，简称鳞癌)中MCP-1表达与肿瘤血管生成的关系。方法应用免疫组化方法检测41例口腔鳞癌和10例正常口腔黏膜MCP-1和CD34的表达，光镜下进行MCP-1表达的分级和微血管的计数。结果口腔鳞癌中MCP-1的表达和微血管计数较正常口腔黏膜均增加(P＜0．01)，在鳞癌中随MCP-1表达的增加，微血管计数增加(P＜0．01)。结论MCP-1在口腔鳞癌中参与了肿瘤的血管生成，但其具体机制和在临床中的意义还需进一步的研究。     

Abbe皮瓣在上唇鳞状细胞癌术后缺损修复中的应用

目的:观察Abbe皮瓣修复上唇鳞状细胞癌切除术后缺损的临床疗效。方法:对5例37~62岁患有上唇鳞状细胞癌的患者进行肿瘤根治性切除,术后形成的继发性上唇缺损选择Abbe皮瓣修复,并进行临床观察。结果:4例患者术后外形良好,功能正常。1例术后出现轻度小口畸形,对进食,语言功能影响不明显,患者不要求治疗。结论:对上唇缺损宽度小于上唇唇长1/2者,治疗选择Abbe皮瓣修复,不仅能恢复口唇特有的红,白唇结构,而且还能恢复其特有的解解剖,生理功能,符合唇缺损的美学修复要求。     

Postoperative irradiation for squamous cell carcinoma of the oral cavity: 35-year experience

BACKGROUND: The purpose of this study was to analyze factors influencing outcome in patients who received postoperative irradiation for advanced squamous cell carcinoma of the oral cavity. METHODS: Between October 1964 and November 2000, 226 patients with 230 previously untreated primary invasive squamous cell carcinomas of the oral cavity were treated postoperatively with continuous-course external beam irradiation. All patients had a minimum follow-up of 2 years (analysis, November 2002). No patient was lost to follow-up. RESULTS: The 5-year actuarial rates of locoregional control by pathologic American Joint Committee on Cancer stage were: stage I, 100%; stage II, 84%; stage III, 78%; and stage IV, 66%. Recurrence of cancer above the clavicles developed in 55 patients (24%). In multivariate analysis of locoregional control, positive margins, vascular invasion, perineural invasion, extracapsular extension, and T classification remained significant. CONCLUSIONS: This article provides additional data defining relatively favorable and unfavorable groups of patients in the postoperative setting. Dose recommendations are re-examined and selectively increased for high-risk patients.     

Importance of tumor thickness and depth of invasion in nodal involvement and prognosis of oral squamous cell carcinoma: a review of the literature

BACKGROUND: Oral mucosa squamous cell carcinoma (OSCC) has locoregional evolution, with frequent neck involvement (the most important parameter for prognosis). There are still many doubts concerning the best way to approach N0 neck disease in early-stage lesions (T1 and T2). Many parameters have been studied to identify N0 patients with a high likelihood of harboring occult node metastases or of having them develop. METHODS: A review of the studies analyzing "tumor thickness"/"depth of invasion" in predicting regional metastases and survival was undertaken. RESULTS: The literature suggests that "tumor thickness"/"depth of invasion" is a reliable parameter for predicting regional nodal involvement and survival in OSCC. CONCLUSIONS: Authors are in substantial agreement regarding the reliability of tumor thickness. The lack of comparable study groups, measurement techniques, and cut-off values points to the need for further studies so as to reach a consensus and to develop therapy protocols that include tumor thickness.