Plasma visfatin levels in severe obstructive sleep apnea—hypopnea syndrome

Background  

Obstructive sleep apnea syndrome (OSAS) is associated with obesity and insulin resistance. Visfatin is an insulin-mimicking adipokine, which is considered a link between obesity and insulin resistance. Aim of this study was to evaluate levels of plasma visfatin in patients with severe OSAS and examine their potential correlation with sleep characteristics and several biochemical parameters.     

visfatin与2型糖尿病

visfatin是在人和小鼠的内脏脂肪中提取出的一种新的脂肪细胞因子,它可以结合并激活胰岛素受体,模拟胰岛素的作用,降低血糖水平,改善胰岛素抵抗。糖尿病的患病人数逐年增加,糖尿病已经成为一个新的流行性疾病。现发现visfatin与糖尿病关系密切,本文将visfatin的结构及在体内的分布以及visfatin与胰岛素受体、血糖水平和胰岛素抵抗和脂代谢紊乱的关系作一综述。     

Change of plasma visfatin level in the population with different glucose tolerances

Objective To investigate the change of plasma visfatin level and the relationship of plasma visfatin level to body mass index (BMI) , waist hip ratio (WHR) , blood glucose, plasma insulin levels as well as other factors in the subjects with different glucose tolerances. Methods Fasting and glucose loading 2 h plasma visfatin levels were assayed by ELISA in patients with type 2 diabetes     

有氧运动对胰岛素抵抗小鼠visfatin表达的影响

目的 探讨高脂饮食诱导的胰岛素抵抗(IR)小鼠visfatin表达的改变及有氧运动对visfatin表达及脂代谢的影响.方法 雄性C57BL/6小鼠24只,按随机数字表随机分为普通饮食组(n=12)和高脂饮食组(n=12),后者喂养8周高脂饲料建立IR模型,再各自分为普通饮食对照组(n=6)、普通饮食运动组(n=6)、高脂饮食对照组(n=6)、高脂饮食运动组(n=6).两个运动组进行6周跑台训练,各组饮食不变.14周后ELISA检测小鼠血清visfatin,Western印迹检测腓肠肌、肝脏及睾周脂肪visfatin表达.结果 8周后,与普通饮食组相比,高脂饮食组空腹胰岛素水平升高,血糖曲线下面积增加(t=6.32,17.44,P均＜0.01).14周后,高脂饮食运动组血清visfatin水平低于高脂饮食对照组,而普通饮食运动组血清visfatin水平高于普通饮食对照组(F=43.59,P＜0.05).高脂饮食运动组骨骼肌、肝脏、内脏脂肪组织中visfatin蛋白表达水平较高脂饮食对照组降低(F=29.33～85.17,P均＜0.05),普通饮食运动组骨骼肌visfatin表达较普通饮食对照组升高(F=52.79,P＜0.05),但肝脏、内脏脂肪组织visfatin蛋白表达在普通饮食组间无明显变化(P均＞0.05).结论 高脂饮食可以增加IR模型动物visfatin水平,有氧运动能有效下调visfatin的表达,改善脂代谢紊乱.     

脂肪因子visfatin与动脉粥样硬化性脑梗死的关系

目的 探讨血清visfatin水平与动脉粥样硬化性脑梗死的关系。方法选取急性脑梗死患者60例,按IMT厚度、斑块的回声特征分为无动脉粥样硬化组（B1组）21例、稳定型斑块组（B2组）17例和不稳定型斑块组（B3组）22例。另取健康查体者35例为对照（A组）。酶联免疫吸附试验测定各组血清visfatin水平,并行NIHSS功能评定,分析visfatin与动脉粥样硬化性脑梗死的关系。结果visfatin水平B1、B2、B3组高于A组（P均〈0．01）,B3组高于B1、B2组（P〈0．01）。visfatin水平与NIHSS评分正相关（r=0．47,P〈0．01）,结论血清高水平visfatin与脑梗死的发病相关,visfatin可作为动脉粥样硬化的独立危险因素。     

老年动脉粥样硬化性脑梗死患者血清visfatin水平变化

目的探讨老年动脉粥样硬化性脑梗死患者血清内脂素(visfatin)水平变化。方法急性脑梗死患者108例根据颈动脉内中膜厚度和斑块回声特征,分为无动脉粥样硬化组32例,稳定斑块组49例,不稳定斑块组27例,同时选取健康者40例作为对照,检测各组血清visfatin水平及美国国立卫生研究院脑卒中量表(NIHSS)评分。结果 4组血清visfatin、NIHSS评分差异显著(P0.05),其中不稳定斑块组血清visfatin和NIHSS评分分别为(94.08±24.19)mg/L和(16.15±3.28)分,明显高于其他组;血清visfatin评估老年脑梗死的受试者工作特征(ROC)曲线下面积(AUC)=0.989(P0.05),截断值为16.21μg/L时,灵敏度为99.10%,特异度为97.60%。血清visfatin评估老年动脉粥样硬化性脑梗死的AUC=0.964(P0.05),截断值为56.93μg/L时,灵敏度为92.10%,特异度为91.67%;血清visfatin与NIHSS评分呈正相关(r=0.460,P0.05)。结论血清visfatin水平的升高可能是老年动脉粥样硬化性脑梗死发生的危险因素。     

Can adipokine visfatin be a novel marker of pregnancy‐related disorders in women with obesity?

Overweight and obesity have become a dangerous disease requiring multiple interventions, treatment and preventions. In women of reproductive age, obesity is one of the most common medical conditions. Among others, obese state is characterized by low‐grade systemic inflammation and enhanced oxidative stress. Increased maternal body mass index might amplify inflammation and reactive oxygen species production, which is associated with unfavourable clinical outcomes that affect both mother and child. Intrauterine growth retardation, preeclampsia, or gestational diabetes mellitus are examples of the hampered maternal and foetoplacental unit interactions. Visfatin is the obesity‐related adipokine produced mainly by the visceral adipose tissue. Visfatin affects glucose homeostasis, as well as the regulation of genes related to oxidative stress and inflammatory response. Here, we review visfatin interactions in pregnancy‐related disorders linked to obesity. We highlight the possible predictive and prognostic value of visfatin in diagnostic strategies on gravidas with obesity.     

Nampt/PBEF/visfatin在呼吸系统疾病的研究应用

尼克酰胺磷酸核糖转移酶(Nampt)又名内脏脂肪素(vis-fatin)和前B细胞克隆增强因子(PBEF)。因为Nampt已经分别被人类及小鼠基因组命名委员会指定为该基因和蛋白质的官方命名,故在本综述中,统一使用官方命名Nampt。由于Nampt具有调节尼克酰胺腺嘌呤二核苷酸(NAD)合成、代谢、炎症反应、细胞通透性、血管生成等多种生物学功能,进而在急性肺损伤、慢性阻塞性肺病、肺癌等多种呼吸系统疾病中发挥重要作用。深入研究Nampt与肺部疾病的关系,可能为相关疾病的诊断和治疗提供新靶点。本文就Nampt在呼吸系统相关疾病中的研究进行综述。     

Determination of serum visfatin levels in patients with Behçet’s disease: A case–control study

Aim of the workBehçet’s disease (BD) is an inflammatory, systemic and chronic disorder with unknown etiology affecting multiple systems. Visfatin is a new adipokine with insulin-mimetic properties and pro-inflammatory function. The serum visfatin levels were evaluated in BD patients to investigate its role in the pathogenesis and clinical manifestations of the disease.Patients and methodsForty BD patients were recruited from the Behçet’s disease clinic at Shiraz University of Medical Sciences in southern Iran and 40 healthy control subjects of matching age, sex and body mass index (BMI) were also included. Serum visfatin level was measured using ELISA.ResultsThe 40 BD patients included 16 males and 24 females. Seventeen had active clinical manifestations; 16 with oral ulcer, 5 with genital ulcer, 6 with arthritis and 2 with uveitis. The mean age of the BD patients was 34.95 ± 9.6 years and mean BMI was 23.98 ± 4.44. There were no significant differences between cases (5.05 ± 3.05 ng/ml) and controls (4.72 ± 2.84 ng/ml) in the visfatin level (p = 0.61). The difference in the visfatin level between patients with active and inactive manifestations did not reach statistical significance (6.13 ± 3.20 and 4.25 ± 2.73, respectively; p = 0.07). There was no significant difference according to the gender of the patients or the presence of clinical manifestations.ConclusionIn view of the results in this study, visfatin levels were not significantly different between cases and controls. There was a tendency to a higher level of serum visfatin in active BD patients compared to the inactive cases; however, the difference was unremarkable.     

Resistin and visfatin: are they valuable enough to be the differential diagnosis in familial Mediterranean fever with acute appendicitis?

Familial Mediterranean fever (FMF) is an autosomal recessive disease which predominantly affects certain ethnic groups mainly Sephardic Jews, Turks, Arabs, and Armenians. Differential diagnosis of an attack of FMF with appendicitis could be difficult in patients presenting with acute abdomen. Circulating levels of resistin and visfatin have been shown to increase in several inflammatory conditions. In this study we aimed to investigate the role of resistin and visfatin in diseases activity by monitoring these adipokines’ levels in patients with FMF (attacks and attack-free period) and acute appendicitis. The study involves four groups: group 1—31 FMF patients at attack (M/F, 14/17), group 2—27 FMF patients at attack-free period (M/F, 9/18), group 3—29 acute appendicitis patients (M/F, 16/13), and group 4—20 healthy controls (M/F, 10/10). Erythrocyte sedimentation rate, C-reactive protein, white blood cell count, fibrinogen, resistin, visfatin, interleukin-1β, interleukin-6, interleukin-10, TNF-α, and IFN-γ were evaluated concurrently. Resistin level could be a useful test in diagnosis of FMF patients in attacks period but not in acute appendicitis as differential diagnosis. Measuring visfatin levels would not give additional information neither for attacks and attack-free period nor FMF attack and appendicitis.     

Relationship between circulating visfatin/NAMPT,nutritional status and insulin resistance in an elderly population – results from the PolSenior substudy

Background

Circulating visfatin/nicotinamide phosphoribosyltransferase (visfatin/NAMPT) levels according to some studies are related to nutritional status and insulin resistance. These associations have not been studied in large elderly populations. Therefore, the aim of our study was to assess the relationships between circulating visfatin/NAMPT levels, nutritional status, and insulin resistance in a large population of the elderly.

Materials and methods

Concentrations of glucose, albumin, creatinine, CRP, interleukin-6, insulin, and visfatin/NAMPT (by ELISA) were assessed, and HOMA-IR calculated in 3050 elderly participants of the PolSenior study.

Results

The highest plasma visfatin/NAMPT levels were observed in obese, as well as in non-diabetic insulin resistant subjects; however there were only significant differences found in women. The regression models showed that plasma visfatin/NAMPT levels decline with age and increased with waist circumference, BMI, and hs-CRP. Waist circumference was better correlated than BMI for visfatin/NAMPT levels in statistical models not adjusted by sex, and just the opposite in models which were. We demonstrated a 0.023 ng/mL increase of Visfatin/NAMPT levels for 1 mg/L increase of hs-CRP, and a 0.007 ng/mL decline for each year of age.

Conclusion

Our study revealed that in elderly subjects, circulating visfatin/NAMPT levels are related to age, nutritional status, especially visceral obesity, and inflammation.     

COPD合并T2DM患者血清visfatin与CRP的关系

目的探讨COPD合并2型糖尿病（T2DM）患者血清内脂素（visfatin）与C反应蛋白（CRP）的关系;研究visfatin与CRP对机体糖脂代谢与炎症免疫的影响。方法用酶联免疫吸附法（ELISA）测定血清visfatin水平;速率散射免疫比浊法检测CRP;全自动生化仪测定生化指标。结果 COPD合并2型糖尿病组血清visfatin与CRP水平较正常对照组明显升高[（36.20±3.62）μg/L/（69.75±8.92）mg/Lvs（15.04±0.71）μg/L/（4.75±2.43）mg/L],有统计学差异（P〈0.05）。结论 COPD合并2型糖尿病患者血清visfatin与CRP水平明显升高。     

血清visfatin水平对OSAHS严重程度的判断和诊断价值

目的探讨阻塞性呼吸睡眠暂停低通气综合征(OSAHS)患者血清内脏脂肪素(visfatin)水平与其严重程度的关系及其诊断价值。方法选择OSAHS患者50例,采用酶联免疫吸附法(ELISA)测定轻、中、重患者血清visfatin、白细胞介素6(IL-6)、白细胞介素8(IL-8)及肿瘤坏死因子α(TNF-α)水平,并与20例正常人对照。结果 OSAHS组患者血清visfatin水平与AHI、IL-6、IL-8、TNF-α均呈正相关(P0.05)。患者血清Visfatin水平明显高于健康对照组(P0.05),轻、中、重度OSAHS组患者血清visfatin水平比较差异有统计学意义(P值均0.05)。以血清Visfatin≥28.01 ng/m L为判别标准,诊断OSAHS的敏感度为82%,特异度为75%。结论血清visfatin水平可以了解OSAHS的严重程度,并有助于诊断。     

肝、结肠和胰腺癌患者血清visfatin的表达及意义

目的研究肝、结肠和胰腺癌患者血清内脏脂肪素(visfatin)的表达变化,探讨其对肿瘤诊断的价值及与营养状况、抗肿瘤治疗的关系。方法采集首次确诊的肝癌、结肠癌、胰腺癌患者手术前后及慢性胃炎、消化性溃疡等对照者的血清样品,采用ELISA和放免法检测血清visfatin、癌胚抗原(CEA)水平,采用全自动生化分析仪检测反映机体营养状况的相关生化指标(如白蛋白、前白蛋白等);同时测量其体质学指标(如身高、体质量、腰围、臀围等)。结果与慢性胃炎、消化性溃疡等患者相比,三组肿瘤患者血清visfatin均显著升高(P均<0.05);肿瘤患者术后血清visfatin较术前显著降低(P均<0.05);随肿瘤分期的进展各组血清visfatin亦随之升高(P均<0.05);CEA、相应生化指标及体质学指标均有不同程度变化。结论 visfatin有望成为一项新的广谱性肿瘤标记物、评估营养状况的新指标及治愈肿瘤的新靶点。     

2型糖尿病非酒精性脂肪肝患者visfatin水平与相关因素分析

目的 探讨visfatin与2型糖尿病(T2DM)及其非酒精性脂肪肝(NAFLD)并发症和相关代谢指标的关系.方法 将80例T2DM患者分为合并NAFLD组50例和单纯T2DM组30例,并选30例健康人做对照.所有的受试者均采用酶联免疫测定法(ELISA)测定空腹血清visfatin浓度;并测定各组的人体指标和血代谢指标水平;并分析各指标间及与NAFLD并发症的相关性.结果 ①T2DM合并NAFLD组、单纯T2DM组及对照组间相互比较血清visfatin浓度差异无显著性(P =0.874;P=1.000).②相关分析显示,T2DM组血清visfatin浓度与WC、TG均呈正相关.③多元线性逐步回归分析显示,TG是血清visfatin独立相关因素.结论 血清visfatin浓度在合并非酒精性脂肪肝的2型糖尿病中无明显变化,但与腹型肥胖及高甘油三酯血症密切相关.     

血清脂肪因子visfatin、A-FABP与老年高血压血压变异性的相关性

目的 探讨血清visfatin、A-FABP与老年高血压血压变异性（BPV）相关性。 方法 选取2017年1月至2019年2月保定市第二医院收治的老年高血压患者（≥ 60岁）235例，采用ELISA法检测血清visfatin、A-FABP水平，分析不同水平组visfatin、A-FABP与老年高血压24 h平均动脉压（24 hMABP）、24 h舒张压变异率（24 hDBPV）、24 h收缩压变异率（24 hSBPV）的相关性。 结果 采用最大选择检验法确定血清visfatin、A-FABP的截断点分别为43.5 ng/ml、20.9 ng/ml，visfatin低水平组的24 hMABP、24 hDBPV、24 hSBPV较高水平组明显降低（均P < 0.01）。A-FABP低水平组24 hDBPV、24 hSBPV、visfatin显著低于高水平组（均P<0.01）。24 hSBPV与年龄、LDL-C、血清visfatin、血清A-FABP呈正相关（均P < 0.01）。24 hDBPV与年龄、血清visfatin、血清A-FABP呈正相关（均P < 0.01）。 结论 年龄、A-FABP、visfatin是BPV增高的独立相关因素。     

阻塞性睡眠呼吸暂停低通气综合征患者血清visfatin变化及其临床意义

目的探讨阻塞性睡眠呼吸暂停低通气综合征（OSAHS）患者血清visfatin变化及其临床意义。方法选择年龄、BM I匹配的OSAHS患者33例（OSAHS组）和单纯肥胖者30例（对照组）;并检测两组血清visfatin、血压、空腹血糖（FPG）、胰岛素（FINS）、血脂,以及AHI、最低血氧饱和度（SaO2）。结果与对照组比较,OSAHS组visfatin、AHI、FINS明显升高,最低SaO2明显降低（P〈0.05或〈0.01）。结论 OSAHS患者血清visfatin升高,visfatin可能是导致OASHS发生、发展的因素。