男性泌尿生殖道淋球菌,沙眼衣原体和解脲支原体混合感染的临床分析

对１９９３年￣１９９６年间的２１９７例男性由性病引起的尿道炎患者进行淋球菌、沙眼衣原体和解脲支原体检测，并对混合感染情况进行分析。结果表明：２１９７例患者总的混合感染率为２１．８％（４７８／２１７９），其中，淋球菌混合感染率为４４．２％，沙眼衣原体为４４．７％，解脲支原体为５８．８％；混合感染特别是解脲支原体的混合感染，在近１￣２年增长迅速，而且９０．３％发生在２０￣４０岁年龄组。提示对性病引起的     

287例男性泌尿生殖道感染病原体分析

为进一步了解目前我国男性泌悄生殖道感染的病因，利用细菌培养、解脲支原体培养、免疫荧光检测沙眼衣原体等方法，对２８７例男性泌尿生殖道感染患者进行病原体检测。结果２８２例检出病原体，其中淋球菌７８例（２７．６５％），解脲支原体４９例（１７．３７％），沙眼衣原体４５例（１５．９５％），较国内其它报道少；而非特异性细菌感染物别是在慢性前列腺炎中增加，尤以金黄色葡萄球菌为甚，共９８例，占病例总数的３４．７５     

168例盆腔炎患者宫颈沙眼衣原体及解脲支原体感染结果分析

目的 研究盆腔炎患者与宫颈沙眼衣原体及解脲支原体感染的相关性。方法 对１６８例盆腔炎患者进行宫颈沙眼衣原体和解脲支原体检测,ＣＴ采用抗原免疫法,ＵＵ采用培养法。结果 １６８例中,ＣＴ和ＵＵ的检出率分别为２５．０％和３０．０％,两者感染的总阳性率为５５．４％,两者混合感染率为１０．１％。结论 提示沙眼衣原体及解脲支原体感染为盆腔炎的重要致病因素,对于盆腔炎的患者应重视进行宫颈沙眼衣原体及解脲支原体检     

尖锐湿疣患者合并解脲支原体沙眼衣原体和淋球菌感染的结果分析

对６５例确诊的尖锐湿疣患者，用ＰＣＲ法检测其泌尿生殖道的淋球菌、沙眼衣原体、解脲支原体。结果显示６５例患者中，有３５例合并淋球菌和（或）沙眼衣原体、解脲支原体感染，合并单一病原体感染者２５例，合并两种病原体混合感染者１０例。３５例合并感染者中，男１２例，女２３例。提示ＳＴＤ患者易合并（两种或两种以上）病原体感染，ＳＴＤ患者应进行全面的病原菌检查，以尽早发现隐匿性感染，及时进行针对性治疗     

1063例STD门诊患者解脲支原体、沙眼衣原体与淋球菌感染情况分析

目的了解本地区STD门诊患者的解脲支原体、沙眼衣原体和淋球菌的感染情况。指导临床诊断、用药。方法采集女性宫颈口分泌物、男性尿道口分泌物分别做淋球菌培养、沙眼衣原体抗原检测和解脲支原体培养。结果1063例STD门诊患者中查出单一淋球菌感染208例,单一衣原体感染172例,单一解脲支原体感染358例,支原体、衣原体混合感染53例,淋球菌、支原体混合感染68例,淋球菌、衣原体感染60例,淋球菌、衣原体、支原体三重感染5例。结论混合感染率比较高,混合感染女性明显高于男性,支原体感染女性明显高于男性。     

尖锐湿疣患者合并解脲支原体沙眼衣原体和淋球菌感染的结果 …

对６５例确诊的尖锐湿疣患者，用ＰＣＲ法检测其泌尿生殖道的淋球菌、沙眼衣原体、解脲支原体。结果显示６５例患者中，有３５例合并淋球菌和（或）沙眼前原体、解脲支原体感染、合并单一病原体感染者２５列，合并两种病原体混合感染者１０例。３５例合并感染者中，男１２例，女２３例。提示ＳＴＤ患者易合并（两种或两种以上）病原体感染、ＳＴＤ患者应进行全面的病原菌检查，以尽早发现隐慝性感染，及时进行针对性治疗。     

323例泌尿生殖道衣原体感染合并淋菌及支原体分析

沙眼衣原体(CT)是引起非淋菌性尿道炎(NGU)及女性生殖系统感染的主要病原体。为了解沙眼衣原体合并淋球菌、支原体感染情况，我们于1997年6月～2002年9月，对于确诊为沙眼衣原体感染患者同时进行淋球菌(NG)、解脲支原体(UU)、人型支原体(MH)的检测，有完整资料共323例，结果报告如下：     

1996～1997年305例淋病患者合并感染交原体及沙眼衣原体分析

在现阶段，淋病是我国很常见的性病之一。近年来，支原体及沙眼衣原体感染引起的非淋球菌性尿道炎／宫颈炎（NGU／NGC）也呈上升趋势。为了解淋病患者合并感染支原体及衣原体情况，我们对1996年及1997年本科性病门诊中有完整资料的305例淋病患者进行了分析并报告如下。l材料与方法1．l；旧床资料：所有患者均来自我科性病门诊。其中1996年191例，1997年114例。年龄18－60岁，平均3015岁。男性251例，女性54例。患者同时检查淋球菌（NG）、解腮支原体山U）、人型支原体（MH）及沙眼衣原体（CT）。所有患者自发病至检查期间未用过药物治…     

非淋菌性尿道炎的病原检查研究

目的：探讨有性乱史女性沙眼衣原体、解脲支原体和人型支原体感染的检查方法。方法：沙眼衣原体抗原检查采用C-C快速法解脲支原体和人型支原体的检查采用培养法。结果：406例被检者，沙眼衣原体抗原阳性者106例（26.10%)解脲支原体和人型支原体培养阳性者266例（65.52%)。结论：有性乱史的女性支原体感染率较高；对非淋菌性尿道炎的病原检查，沙眼衣原体抗原采用C-C快速法检查，解脲支原体和人型支原体采用培养法检查均较简便、快速、实用。     

广州地区性病门诊细菌性阴道病的现状调查

为了解广州地区性病门诊女性患者细菌性阴道病（BV）的发病情况，用BV快速检测方法和诊断标准方法对2000年性病门诊725例女性患者进行检测；并对确诊的BV患者做淋球菌、衣原体、支原体、滴虫和念珠菌检测。结果725例病人确诊为BV者131例，患病率为18．07％（131／725）。131例BV患者中有113例合并其它病原菌感染。其合并感染率为86．26％（113／131）。其中解脲支原体(Uu)占首位（38．93％），其次为淋球菌（14．50％），第3为Uu、Mh混合感染（9．90％）。BV快速检测方法阳性预测价值不高（57．60％），不能用于确诊。     

Etiology of urethritis in Thai men

The etiology of urethritis was determined for 303 Thai men with urethral discharge containing 5 or more polymorphonuclear cells (PMN)/high power field (hpf) and 132 men with a discharge containing less than 5 PMN/hpf. Neisseria gonorrhoeae was isolated significantly more often from men with greater than or equal to 5 PMN/hpf than from men with less than 5 PMN/hpf (42% vs 1%, P less than .0001). Chlamydia trachomatis was also isolated more often from patients with greater than or equal to 5 PMN/hpf than from men with less than 5 PMN/hpf (16% vs 8%, P less than .03). Ureaplasma urealyticum was isolated with nearly equal frequency from both groups of patients (45% vs 37%). Among men with a urethral exudate containing greater than or equal to 5 PMN/hpf, N. gonorrhoeae was isolated as the only pathogen from 19% and in combination with C. trachomatis or U. urealyticum in 23% of these men. C. trachomatis or U. urealyticum, but not N. gonorrhoeae, was isolated from 30%, and no pathogen was isolated from 28% of these men. Among men with urethral exudate containing less than 5 PMN/hpf, N. gonorrhoeae was isolated from only 1%, C. trachomatis or U. urealyticum from 41%, and no pathogen from 58%. These findings suggest that all Thai men with urethral discharge containing greater than or equal to 5 PMN/hpf should be treated for non-gonococcal urethritis and for gonococcal urethritis if gram-negative diplococci are demonstrated on gram stain of the urethral discharge. Men with urethritis with less than 5 PMN/hpf should be treated for only non-gonococcal urethritis.     

Serotypes of Ureaplasma urealyticum isolated from patients with nongonococcal urethritis and gonorrhea and from asymptomatic urethral carriers

Ureaplasma urealyticum strains isolated from urethral specimens of 112 patients with nongonococcal urethritis, 17 with gonorrhea, and 33 asymptomatic carriers (controls) were tested by the growth-inhibition test with standard ureaplasma antisera (serotypes 1-8). Cases with more than one serotype (two to four types) were encountered in 36.6% of patients with nongonococcal urethritis, 29.4% of those with gonorrhea, and 21.2% of controls. The most frequently recovered serotypes were type 4 from patients with nongonococcal urethritis (57.1%) and type 2 from patients with gonorrhea and controls (35.2% and 36.3%, respectively). The difference in frequency of serotype 4 between patients with nongonococcal urethritis and controls was statistically significant (P less than .001). An additional serotyping with types 9 and 10 antisera of the nontypable strains from patients with nongonococcal urethritis and controls (17.8% and 21.2%, respectively) revealed three type-9 strains. The results show a need for utilization of serotyping of U. urealyticum in studies of the epidemiology and transmissibility of genital infections with U. urealyticum.     

Tetracycline in nongonococcal urethritis. Comparison of 2 g and 1 g daily for seven days.

In a previous study treatment with minocycline 100 mg orally every day for seven days was as effective for nongonococcal urethritis (NGU) as 200 mg for seven days or 100 or 200 mg for 21 days. In this prospective, randomised study men with NGU received tetracycline either 500 mg or 250 mg four times daily for seven days. of 200 men initially enrolled, Chlamydia trachomatis was isolated from 40% and Ureaplasma urealyticum from 48%. Eight of 10 homosexual men compared with 39 (21%) of 190 bisexual or heterosexual men had negative culture results for both C trachomatis and U urealyticum (x2 = 15.5, P < 0.0005). U urealyticum was isolated more frequently from chlamydia-negative men and from men with 10 or fewer sex partners during their lifetime. Both regimens were equally effective in their in-vivo activity against C trachomatis and U urealyticum. Failure rates were similar with the two regimens. More obvious failure with purulent or profuse mucoid discharge and pyuria occurred more frequently with the 250-mg regimen (20% of 76 men on the 250-mg regimen compared with 7% of 67 men on the 500-mg regimen; x2 = 4.45, P < 0.05). Failure occurred more frequently in men who were initially chlamydia-negative and in men in whom U urealyticum persisted after medication. Thus, the 250-mg regimen appeared to be as effective as the 500-mg regimen in the initial treatment of NGU. However, one-third of men had persistent or recurrent urethritis with these regimens, and there is a need for antimicrobial agents with greater in-vivo activity, especially against chlamydia-negative NGU.     

Decreased incidence of postgonococcal urethritis following minocycline treatment compared to penicillin/spectinomycin

Two groups of 30 patients each suffering from gonococcal urethritis were treated either with minocyclin for 7 days or with a single administration of penicillin or spectinomycin, respectively. 30% of them revealed an additional infection with C. trachomatis or U. urealyticum. On the 8th day, we observed remaining symptoms in only 20% of the patients treated with minocyclin, but in 40% of the group treated with penicillin/spectinomycin.     

Colonization of sexually abused children with genital mycoplasmas

Although sexually abused children are usually evaluated only for the presence of infections with Neisseria gonorrhoeae and Treponema pallidum, they are also at risk for acquiring other sexually transmitted organisms prevalent in the adult population. Accordingly, we examined pharyngeal, anorectal and genital specimens from 50 children who had been sexually abused and from 40 healthy children who served as controls; these specimens were cultured for Mycoplasma hominis and Ureaplasma urealyticum. M. hominis was isolated from the anorectal and vaginal cultures of 11 (23%) and 16 (34%), respectively, of 47 abused girls as compared with three (8%) and six (17%), respectively, of 36 controls. U. urealyticum was isolated from the anorectal and vaginal cultures of nine (19%) and 14 (30%), respectively, of the abused girls as compared with one (3%) and three (8%), respectively, of 36 controls. Colonization with genital mycoplasmas was not associated with any symptoms.     

Condylomata acuminata-associated STD infections of the urethra of the male. A comparative epidemiologic study

Urethral swabs from 322 men without urethritis, 73 of whom had venereal warts (condylomata acuminata, CA) and 249 who had none, were investigated between 1981 and 1984 by microscope and culture for the presence of Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma species, Trichomonas vaginalis, Garderella vaginalis, B streptococci, aerobic pathogenic bacteria and Candida species. The isolation frequencies in patients with and without CA were as follows: N. gonorrhoea: with CA 0%, without 0.4%; C. trachomatis: with CA 6%, without 4.4%; U. urealyticum in high CFU: with CA 15%, without 17.7%; Mycoplasma spp. in high CFU: with CA 6%, without 4%; T. vaginalis: with CA 0%, without 0.4%; G. vaginalis: with CA 4%, without 5%; B streptococci: with CA 4%, without 6%; Candida spp. only in low CFU: with CA 3%, without 2.4%.--The results indicate that as far as the isolation frequencies of sexually transmittable pathogens in the urethra are concerned, there are no significant differences between patients with CA and asymptomatic patients presenting to an STD department. However, N. gonorrhoeae was significantly less frequently isolated and C. trachomatis and U. urealyticum significantly more frequently isolated in our patients than has been reported in previous studies.     

应用A7琼脂法检测泌尿生殖道支原体

目的：评价A7琼脂法在检测泌尿生殖道标本中解脲脲原体(Ureaplasma urealyticum,Un)和人型支原体(Mycoplsma hominis，Mh)中的意义，为临床诊断Uu和Mh的感染提供实验依据。方法：取接种了泌尿生殖道标本的运送培养基直接接种于A7琼脂，在5％CO2和95％N2的气体环境中培养24h～48h后，低倍镜下观察特征性菌落，计数其菌落形成单位(Colony Forming Units,CFU)。结果：286例泌尿生殖道标本中分离到Uu 102例，Mh29例，混合感染27例，Uu计数≥10^4CFU75例，Mh计数≥10^4CFU24例。结论：A7琼脂法简便可行，结果直观可信，适用于临床泌尿生殖道中Uu、Mh的检测。     

解脲脲原体生物群及基因型与黏液脓性宫颈炎相关性的研究

目的 探讨解脲脲原体生物群及基因型与女性非衣原体非淋球菌感染的黏液脓性宫颈炎的相关性。方法 女性非衣原体非淋球菌感染的黏液脓性宫颈炎患者及正常人群两组,对解脲脲原体培养阳性标本应用PCR-SSCP方法检测解脲脲原体的生物群及基因型。结果 解脲脲原体生物一群中的基因3/14型在两组中的检出率均最高,分别为30.98%（57/184）和43.42%（33/76）。黏液脓性宫颈炎患者组中生物二群的解脲脲原体基因2B型的检出率为16.30%（30/184）明显高于正常人组的6.58%（5/76）,差异有统计学意义（χ2 = 4.367,P ＝ 0.037）。而健康体检组中主要以生物一群中的基因1、3/14、6型的单型别定植为主占81.58%。结论 解脲脲原体生物二群中的基因2B型可能与性病门诊就诊者中的非衣原体非淋球菌感染的黏液脓性宫颈炎有一定相关性。     

Prevalence of Chlamydia trachomatis and other micro-organisms in women seeking abortions in Pittsburgh, Pennsylvania, United States of America.

The prevalence of Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, group B streptococcus, herpes simplex virus, and Neisseria gonorrhoeae from cervical cultures obtained from 210 women seeking abortion in Pittsburgh, Pennsylvania, United States of America was 9.3%, 72.9%, 25.2%, 4.3%, 0.9%, and 0.9% respectively. Cultures from 40/203 (19.7%) patients failed to produce any of these organisms. C trachomatis isolation was not associated with age, race, marital status, average family income, number of sexual partners, history of gonorrhoea or syphilis, or previous pregnancies, live births, or abortions, and 82.4% of women with chlamydial infections had had no urogenital symptoms in the preceding six months. The highest concentration of U urealyticum was 10(5) colour changing units (ccu)/ml, and about half of the positive ureaplasma cultures produced less than 10(3) ccu/ml of this organism. Screening for C trachomatis, is encouraged to prevent neonatal morbidity and the common complication of pelvic inflammatory disease after abortion.