两种饲料对丝光绿蝇蛹长和羽化率的影响

目的 观察比较两组丝光绿蝇室内连续饲养的蛹期形态和羽化率。方法 将丝光绿蝇引入室内，分成鱼粉组和麦麸组两种幼虫饲料在室内进行连续饲养，对两组各代蛹期的蛹长、羽化率等进行比较观察，结果 第204代蛹长鱼粉组显著大于麦麸组；第5代蛹长鱼粉组与麦麸组差异无显著性。第1－4代羽化率两组差异无显著性，第5代羽化率鱼粉组显著性高于麦麸组。结论 鱼粉组蛹形态、羽化率等指标好于麦麸组，提示鱼粉组饲料饲养效果好于麦麸组饲料。     

不同饲料和湿度对丝光绿蝇羽化率的影响

目的 观察比较不同饲料和蛹期湿度对室内连续饲养的丝光绿蝇羽化率的影响。方法 将野外丝光绿蝇引入室内，分别以麦麸为主（A组）和以鱼粉为主（B组）的两种幼虫饲料在室内进行连续饲养，将各代蛹分别置于瓶中羽化，瓶内置湿棉球，另外挑取鱼粉粗蝇蛹于瓶中，但不置湿棉球（C组），观察各组各代羽化率。结果 鱼粉组（B组）与麦麸组（A组）第1－4代羽化率经t－检验两组间差异无显著性，第5代鱼粉组（B组）羽化率显著性高于麦麸组（A组），将鱼粉组饲养的蝇蛹置于瓶中置湿棉球（B组）与不置湿棉球（C组）作比较，两组间各代差异均无显著性。组内各代间比较，各组第2－5组内代间羽化率差异均无显著性，麦麸组（A组）第1代羽化率显著高于其第2－5代，鱼粉组（B组）第1代羽化率显著高于其第2，3，5代。结论 幼虫饲料添加渔粉对丝光绿蝇室内连续饲养羽化率的影响主要表现在饲养后期，而在饲养初期无显著性影响。在适宜的湿度范围内，丝光绿蝇的羽化率不受蛹期相对湿度变化的影响。     

不同饲料和湿度对丝光绿蝇羽化率的影响

目的　观察比较不同饲料和蛹期湿度对室内连续饲养的丝光绿蝇羽化率的影响。　方法　将野外丝光绿蝇引入室内 ,分别以麦麸为主 ( A组 )和以鱼粉为主 ( B组 )的两种幼虫饲料在室内进行连续饲养 ,将各代蛹分别置于瓶中羽化 ,瓶内置湿棉球 ,另外挑取鱼粉组蝇蛹于瓶中 ,但不置湿棉球 ( C组 ) ,观察各组各代羽化率。　结果　鱼粉组 ( B组 )与麦麸组 ( A组 )第 1～ 4代羽化率经 t-检验两组间差异无显著性 ,第 5代鱼粉组 ( B组 )羽化率显著性高于麦麸组 ( A组 ) ,将鱼粉组饲养的蝇蛹置于瓶中置湿棉球 ( B组 )与不置湿棉球 ( C组 )作比较 ,两组间各代差异均无显著性。组内各代间比较 ,各组第 2～ 5组内代间羽化率差异均无显著性 ,麦麸组 ( A组 )第 1代羽化率显著高于其第 2～ 5代 ,鱼粉组 ( B组 )第 1代羽化率显著高于其第 2、3、5代。　结论　幼虫饲料添加鱼粉对丝光绿蝇室内连续饲养羽化率的影响主要表现在饲养后期 ,而在饲养初期无显著性影响。在适宜的湿度范围内 ,丝光绿蝇的羽化率不受蛹期相对湿度变化的影响。     

两种饲料对丝光绿蝇产卵的影响

目的 观察比较鱼粉、麦麸两种幼虫饲料对丝光绿蝇室内连续饲养的各代成虫产卵的影响。方法 将丝光绿蝇引入室内，分成两组，即鱼粉组和麦麸组，在室内进行连续饲养，分别观察记录两组室内第1－5代成虫的产卵天数和产卵次数，并计算产卵指数，进行比较。结果 鱼粉组与麦麸组成虫室内第1、5代产卵天数、产卵次数、产卵指数两组间差异无显著性，第2－4代产卵天数、产卵次数、产卵指数，鱼粉组显著大于麦麸组。结论 鱼粉组产卵力好于麦麸组，可能提示鱼粉饲料更有利于丝光绿蝇的发育与繁殖。     

两种饲料对丝光绿蝇产卵的影响

目的观察比较鱼粉、麦麸两种幼虫饲料对丝光绿蝇室内连续饲养的各代成虫产卵的影响。方法将丝光绿蝇引入室内 ,分成两组 ,即鱼粉组和麦麸组 ,在室内进行连续饲养 ,分别观察记录两组室内第 1～ 5代成虫的产卵天数和产卵次数 ,并计算产卵指数 ,进行比较。结果鱼粉组与麦麸组成虫室内第 1、5代产卵天数、产卵次数、产卵指数两组间差异无显著性 ,第 2～ 4代产卵天数、产卵次数、产卵指数 ,鱼粉组显著大于麦麸组。结论鱼粉组产卵力好于麦麸组 ,可能提示鱼粉饲料更有利于丝光绿蝇的发育与繁殖。     

室内饲养丝光绿蝇的生物学特征观察

目的　观察比较丝光绿蝇室内连续饲养的各代生物学特性。　方法　将丝光绿蝇引入室内 ,采用改良的饲养方法对其进行室内连续饲养 ,对其室内第 1～ 5代生活史各期的生长发育、产卵、繁殖等各种生物学特性进行观察分析。　结果　结果经 t检验发现 ,第 3、4代间蛹重、蛹长差异均无显著性 ,但蛹重、蛹长都显著性 >第 2代及第 5代 ;第 2、5代间蛹重及蛹长差异均无显著性。第 1代羽化率显著高于第 2、3、5代 ,第 2～ 5代间羽化率差异无显著性。成虫头宽第 1代均显著 >其第 2～ 5代。第 1、2、4代雄性略多于雌性 ,第 5代雄性稍少于雌性 ,代间比较差异均无显著性。成虫室内第 1代产卵天数显著少于第 2、3、5代 ,与第 4代差异无显著性 ,第 2～ 5代间产卵天数差异均无显著性 ;产卵次数及产卵指数室内第 1～ 5代间均无显著性差异。丝光绿蝇室内各代饲养的生活史各期发育时间 ,卵期第 2～ 5代 <1d,幼虫期 6～ 7d,蛹期 6 d,产卵前期 6～ 7d。　结论　本次实验丝光绿蝇引入室内连续饲养的各代生物学特性比较稳定。     

丝光绿蝇抗菌物质的诱导

目的探讨针刺体壁诱导丝光绿蝇产生抗菌物质的规律。方法室内饲养的丝光绿蝇三龄幼虫，经针刺体壁处理后，分别于第0、12、24、36、48、60、72、84h记录幼虫活率，并提取其血淋巴，以金黄葡萄球菌作指示菌，采用平板法，作抑菌试验，37℃恒温24h，观察抑菌圈，并以抑菌圈直径表示抗菌活力的大小，借以指示诱导丝光绿蝇抗菌物质的产生规律。结果经针刺诱导后，丝光绿蝇幼虫活率逐渐降低，血淋巴对金黄葡萄球菌的抑菌活力峰出现在诱导后第48h。结论针刺体壁可诱导丝光绿蝇产生抗菌物质，免疫血淋巴收集在诱导后48h最为合适。     

吗啡对丝光绿蝇幼期发育的影响

法医昆虫毒理学是法医昆虫学和毒理学相结合的一门新兴学科的分支。以法医昆虫如蝇蛆、蝇蛹为毒理学材料的优点是：当传统的毒理学材料如血液、液尿和同体器官不复可得时，它们仍然可存在相当长时间，分析中不受尸体分解产物的干扰，有关分析结果，可以帮助推断死亡原因，修正死后间隔时间。本文研究在室内28℃恒温条件下吗啡对丝光绿蝇幼虫期发育的影响。现报告如下。     

丝光绿蝇致胃肠蝇蛆病1例

本文报道了1例由丝光绿蝇引起的胃肠蝇蛆病。     

温度对丝光绿蝇寿命的影响

蝇类是一种变温的节肢动物,蝇的生长发育受温度、湿度、食物、种群密度等多种自然因素和生物因素影响,其中温度对蝇幼虫、蛹的生长及成蝇的羽化有着非常重要的作用[1],而且成蝇对温度也十分敏感,温度影响成蝇的寿命、活动能力、繁殖能力及季节分布等,本研究观察了21℃、28℃恒温条件下雌、雄丝光绿蝇成蝇的寿命。1材料与方法丝光绿蝇为河北医科大学校园内以家兔内脏诱捕获得,在实验室内连续饲养3代,第3代成蝇作为实验蝇。收集24h内羽化的成蝇600只,分为2笼,每笼雌雄之比为1∶1。其中一笼放入21以℃恒温箱培养,另外一笼放入28℃恒温箱培养,相…     

丝光绿蝇抗菌物质针刺诱导及其性质研究

目的 探讨针刺体壁对丝光绿蝇抗菌物质的诱导表达。并研究丝光绿蝇免疫血淋巴的性质。方法 丝光绿蝇3龄幼虫针刺体壁处理后,分别于第0、12、24、36、48、60、72、84h提取血淋巴,以金黄葡萄球菌为指示菌,采用平板滤纸片法作抑菌试验,用于显示丝光绿蝇抗菌物质的诱导表达,以抑菌圈直径表示抑菌活力的大小。提取诱导后48h的血淋巴,设置3μl、5μl、7μl不同的血淋巴加样量实验组,记录不同加样量对金黄葡萄球菌的的抑菌效果;设置不同稀释浓度,记录不同浓度的血淋巴对金黄葡萄球菌的抑菌活力。诱导后48h的血淋巴置于冰箱保存后,设置36℃和100℃不同解冻温度,观察不同温度解冻的血淋巴对金黄葡萄球菌和大肠杆菌的抑菌效果。诱导后48h的血淋巴,100℃水浴3min,观察其对金黄葡萄球菌的抑菌活力。提取诱导后48h的血淋巴,观察其对大肠杆菌、金黄葡萄球菌、白色葡萄球菌、变形杆菌、伤寒杆菌、副伤寒杆菌、枯草杆菌、溶壁微球菌等8种细菌的抑菌效果。结果 丝光绿蝇幼虫针刺体壁处理后,对金黄葡萄球菌的抑菌活力高峰出现在处理后48h。平板滤纸片法抑菌试验中,以加7μl血淋巴的抑菌效果最佳。诱导后48h的血淋巴置于冰箱保存后,36℃和100℃不同温度解冻,对金黄葡萄球菌和大肠杆菌均有抑菌效果,36℃解冻效果好于100℃。     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.