鞘内注射布托啡诺对福尔马林诱导大鼠脊髓背角NMDAR的影响

目的观察鞘内注射布托啡诺对福尔马林炎性痛大鼠脊髓背角N-甲基-D-天冬氨受体（NMDAR）表达的影响。方法健康雄性SD大鼠20只,体重220～280g。随机分为4组（n=5）：对照组（C组）,鞘内注射生理盐水组（N组）,鞘内注射12．5μg布托啡诺组（B1组）,鞘内注射25μg布托啡诺组（B2组）。在大鼠的左后足掌面皮下注射5％福尔马林50μl致痛前30min,N组、B1组和B2组分别注射生理盐水、12．5μg布托啡诺、25μg布托啡诺,C组在福尔马林注射前不注射任何试剂。记录福尔马林诱导出注射后爪的双相理毛行为时间（在福尔马林注射后第1时相,0—5min;第2时相,10—60min）。所有大鼠均在注射后2h处死,用免疫组化法测定大鼠L5节段脊髓背角NMDA受体的表达。结果与C组相比,N组和B1组对福尔马林诱导的第1和2时相．影响差异无统计学意义（P〉0．05）,3组脊髓背角NMDA受体表达均增加,相比较差异无统计学意义（P〉0．05）。与C组比较,B2组引起第1和2时相的后爪理毛行为的总计反应时间明显减少,并显著降低福尔马林致痛大鼠L5脊髓背角NMDA受体表达（P〈0．05）。结论鞘内注射布托啡诺能够对福尔马林诱导的疼痛行为产生明显的抑制作用,并具有剂量依赖性,其镇痛机制与通过抑制NMDA受体激活产生有关。     

不同剂量布托啡诺用于无痛人流术的效果观察

目的 比较观察不同剂量布托啡诺用于无痛人流术的临床效果.方法 选择150例要求无痛人流术的早孕患者,分三组,各50例：布托啡诺＋丙泊酚组为实验组（B组）,其中B1组布托啡诺剂量为1 mg,B2组布托啡诺剂量为0.5 mg,丙泊酚组（P组）为对照组,观察B组给予布托啡诺后患者呼吸、神志的改变,控制麻醉深度,记录丙泊酚注药后100 s内SpO2最低值、手术时间、丙泊酚总量、意识恢复时间及注射痛、术中体动、术后恶心呕吐、躁动发生率和唤醒时视觉模拟评分（VAS）.结果 给予布托啡诺后B1组主诉呼吸费力、头昏的发生率明显高于B2组（P〈0.01）,三组手术时间、SpO2最低值、恶心呕吐发生率差异无统计学意义（P〉0.05）,但丙泊酚总量、注射痛、术中体动、术后躁动发生率、VAS评分P组明显高于B组（P〈0.01）,而意识恢复时间B1组较B2、P组比较明显延长（P〈0.01）.结论 在无痛人流术中,布托啡诺0.5 mg联合丙泊酚,可明显减轻注射痛,有效抑制宫缩痛,减少术中体动、术后躁动等不良反应且不影响术后意识恢复.     

静脉注射布托啡诺防治剖宫产术中及术后寒战的效果观察

目的评价布托啡诺对剖宫产患者术中及术后寒战的防治作用。方法选择90例择期在腰硬联合麻醉下行剖宫产手术患者，ASAⅠ～Ⅱ级，随机分为三组：布托啡诺组（B组）、曲马多组（T组）、对照组（N组），每组30例。胎儿娩出后B组给予布托啡诺（0．01mg／kg）加生理盐水10m1静脉注射，T组给予曲马多（1mg／kg）加生理盐水10ml静脉注射，N组给予生理盐水10ml静脉注射。分别记录用药前及用药后3、5min时循环、呼吸的变化，寒战的评分和不良反应。结果三纽患者循环呼吸的变化无统计学差异：与N组比较B组和T组患者均于用药后5min寒战停止，但T组出现恶心、呕吐及复发寒战不良反应发生率较B组高，两组有统计学差异（P〈0．05）。结论布托啡诺（0．01mg／kg）在治疗剖宫产患者术中及术后寒战中效果优于曲马多（1mg／虹）。     

小剂量布托啡诺对剖宫产术患者清醒镇静的效果评价

目的：探讨全程应用小剂量布托啡诺对剖宫产患者清醒镇静的影响及安全性。方法：择期行剖宫产患者160例,随机分为4组（n=40）,即A组（n=布托啡诺5ug．kg^-1）,B组（布托啡诺7．5ug．kg-1）,C组（布托啡诺10ug．kg^-1）,D组（未用布托啡诺）,于入室即刻（T1）、切皮即刻（T2）、胎儿娩出断脐时（T3）、和术毕（T4）记录SP、DP、HR、SPO2和血糖;记录T2、T3、T4产妇镇静满意率,镇静评分采用Ramsay评分法。结果①A组T2、T3、T4镇静满意率在55．0％～75％之间,B组在80％～95％之间,C组在85％～92．5％之间。B、C组镇静满意率高于A组;②D组T2—3时SP、HR高于A、B、c组（P〈O．01）,D组T2—4时血糖高于A、B、c组（P〈O．01）。结论：①临产前静脉注射布托啡诺以7．5ug．kg。为宜,有效减轻临产前剖宫产患者焦虑、恐惧情绪;减少术中恶心呕吐、牵拉反应和寒颤发生。     

不同剂量布托啡诺用于无痛人流术的效果观察

目的 比较观察不同剂量布托啡诺用于无痛人流术的临床效果.方法 选择150例要求无痛人流术的早孕患者,分三组,各50例:布托啡诺+丙泊酚组为实验组(B组),其中B1组布托啡诺剂量为1 mg,B2组布托啡诺剂量为0.5 mg,丙泊酚组(P组)为对照组,观察B组给予布托啡诺后患者呼吸、神志的改变,控制麻醉深度,记录丙泊酚注药后100 s内SpO2最低值、手术时间、丙泊酚总量、意识恢复时间及注射痛、术中体动、术后恶心呕吐、躁动发生率和唤醒时视觉模拟评分(VAS).结果 给予布托啡诺后B1组主诉呼吸费力、头昏的发生率明显高于B2组(P<0.01),三组手术时间、SpO2最低值、恶心呕吐发生率差异无统计学意义(P>0.05),但丙泊酚总量、注射痛、术中体动、术后躁动发生率、VAS评分P组明显高于B组(P<0.01),而意识恢复时间B1组较B2、P组比较明显延长(P<0.01).结论 在无痛人流术中,布托啡诺0.5 mg联合丙泊酚,可明显减轻注射痛,有效抑制宫缩痛,减少术中体动、术后躁动等不良反应且不影响术后意识恢复.     

布托啡诺复合罗哌卡因用于乳腺癌术后硬膜外镇痛的效果及安全性

目的 观察布托啡诺复合罗哌卡因用于乳腺癌术后硬膜外镇痛的效果与不良反应.方法 选择60例在连续硬膜外麻醉下行乳腺癌改良根治术的患者,随机分为布托啡诺复合罗哌卡因镇痛组（B组,n=30）和吗啡复合罗哌卡因镇痛组（M组,n=30）,术后硬膜外镇痛,B组给予20 mg/L布托啡诺＋1 g/L罗哌卡因 M组给予20 mg/L吗啡＋1 g/L罗哌卡因.两组所用镇痛泵容量均为100 ml,背景剂量2 ml/h,自控给药量2 ml/h,锁定时间15 min.术后持续监测血压、心率、血氧饱和度.观察术后5 h、10 h、20 h、30 h不同时段疼痛评分及有无恶心呕吐、皮肤瘙痒、呼吸抑制等不良反应.结果 两组患者术后不同时段镇痛效果,VAS评分差异无统计学意义（P〉0.05）.对平均动脉压、心率、血氧饱和度等生理指标的影响,两组差异无统计学意义.B组恶心呕吐、皮肤瘙瘁的发生率明显低于M组（P〈0.05）,两组均未出现明显的呼吸抑制.结论 布托啡诺复合罗哌卡因用于乳腺癌术后硬膜外镇痛效果满意,安全,不良反应少.     

酒石酸布托啡诺复合丙泊酚用于控制无痛人工流产术后宫缩痛的临床观察

目的观察酒石酸布托啡诺（以下简称布托啡诺）复合丙泊酚用于控制无痛人工流产术后宫缩痛的临床效果。方法将200例自愿行无痛人工流产术患者随机分为两组：单纯丙泊酚组（A组）和小剂量布托啡诺（1mg）复合丙泊酚组（B组）,每组各100例。A组静脉注射丙泊酚2mg/kg,B组缓慢静脉注射1mg布托啡诺后静脉注射丙泊酚2mg／kg。结果B组患者意识恢复后0、10、20min视觉模拟评分（VAS）为差（VAS〉5分）的例数分别为8．4、1例,明显少于A组的31、21、12例,两组比较差异有统计学意义（P〈0．05）。结论小剂量布托啡诺复合丙泊酚用于控制无痛人工流产术后宫缩痛安全有效。     

布托啡诺与吗啡复合罗哌卡因用于剖宫产硬膜外镇痛的效果比较

目的比较布托啡诺与吗啡复合罗哌卡因用于剖宫产术后硬膜外镇痛的效果,探讨更佳的硬膜外镇痛药液配方。方法将150例(ASAI~Ⅱ级)采用腰麻-硬膜外联合麻醉剖宫产的产妇随机分成三组,吗啡(M组)、布托啡诺1(B1组)、布托啡诺2(B2组),每组各50例。M组应用0.13%罗哌卡因加0.008%吗啡;B1组应用0.13%罗哌卡因加0.0033%布托啡诺;B2组应用0.13%罗哌卡因加0.005%布托啡诺;三组药液均加入昂丹司琼4mg。均采用负荷量加持续剂量加自控给药的模式。对三组病例的镇痛效果(VAS评分)、舒适度(BCS评分)、镇静(Ramesay评分)和所出现的并发症进行总结分析。结果三组均能取得良好的镇痛效果,除8h点M组优于B1组,VAS评分、舒适度BCS评分差异有统计学意义(p<0.05)以外,其它时间点VAS评分、舒适度BCS评分、Ramesay评分均无统计学意义(p>0.05)。不良反应的发生率B1组明显少于M组,有统计学意义(p<0.05);B2组优于M组,但两组之间无明显差异。结论三组均取得了良好的镇痛效果,但是0.13%罗哌卡因加0.0033%布托啡诺大大减少了不良反应的发生。     

布托啡诺用于硬膜外麻醉后寒战预防性用药的临床研究

目的观察布托啡诺用于硬膜外麻醉寒战反应的预防性用药临床效果。方法 150例ASAⅠ-Ⅱ级在硬膜外麻醉后的患者,随机分为对照组、度氟合剂组、布托啡诺组,每组50例。麻醉效果确切后(即硬膜外注药15 min后),对照组没有用药,度氟合剂组给予度冷丁1 mg/kg,氟哌利多0.05 mg/kg,布托啡诺组给予布托啡诺0.1 mg/kg。记录药物应用前后MAP、HR、SpO2的变化及不良反应情况,观察给药术中、术毕、术后6 h内寒战发生率。结果度氟合剂组在给药后MAP、HR升高显著(均p﹤0.05),布托啡诺组不明显(均p﹥0.05),给药后度氟合剂组、布托啡诺组寒战发生率与对照组比较有显著性差异(均p﹤0.05),术后6 h内寒战发生率对照组、度氟合剂组明显高于布托啡诺组(均p﹤0.05)。结论布托啡诺作为硬膜外麻醉后预防性用药能明显减少寒战反应发生,且副作用小,临床应用是安全可行的。     

布托啡诺预防和治疗开胸手术患者麻醉苏醒期躁动的临床观察

目的探讨椎托啡诺在预防和治疗开胸手术患者麻醉苏醒期躁动的有效性及安全性。方法120例ASAⅠ～Ⅱ级的择期开胸手术患者，随机双盲分为三组，每组40例，即B组（布托啡诺组）、F组（芬太尼组）和N组（对照组）。在缝合皮肤前静脉注射拟观察的药物，观察患者在拔管即刻和在苏醒室的躁动及镇静评分、药物不良反应、拔管时间及缓解情况等，N组患者若发生躁动则静脉注射布托啡诺20μg/kg.结果三组患者在拔管时间及苏醒时间差异无统计学意义，N组拔管后躁动的发生率明显高于F组及B组，差异有统计学意义（P〈0．01）。B组的镇静评分较高，但是没有过度镇静或苏醒延迟的发生，各组间比较差异有统计学意义（P〈0．05）。在苏醒室F组追加芬太尼后躁动缓解率为50．0％（8116），而B组追加布托啡诺后躁动缓解率为100．0％（6／6）。N组发生躁动的患者经静脉注射布托啡诺后躁动缓解或减轻率为88．0％（22／25），F组8例患者追加芬太尼后有5例出现一过性的呼吸抑制，而B组及N组均无呼吸抑制的发生。结论布托啡诺是预防和治疗术后躁动的理想选择，而芬太尼并不能有效防治术后躁动。     

缬沙坦/氨氯地平和依贝沙坦/双氢克尿噻联合治疗老龄高血压疗效的研究

Objective To compare the clinical effect of valsartan/amlodipine combination or irbesartan/hydrochlorothiazide(HCTZ)combination in very elderly hypertensives.Methods After a 4-week placebo period,94 hypertensives,aged 75-89 years were random given valsartan 160 mg/amlodipine 5 mg or irbesartan 300 mg/HCTZ 12.5 mg for 24 weeks according to a rospective study.After 4 weeks,amlodipine or HCTZ was doubled in non-responders.Patients were checked every 4 weeks.At each visit,sitting,lying and standing blood pressure(BP),systolic BP(SBP)and diastolic BP(DBP)were measured. At the end of placebo period and treatment period,electrolytes and uric acid were evaluated.Results Blood pressure was significantly decreased in both treatment groups,however,there was no statistical significance between two groups.BP changes from lying to standing position were significantly greater in the irbosartan/HCTZ group(-17.2/-9.1 mmHg)than that in the valsartan/amlodipine group(-10.1/-1.9 mmHg,t=2.14,P＜0.05 for SBP and t=3.11,P＜0.01 for DBP vs.irbesartan/HCTZ).Potassium significantly decreased and uric acid significantly increased(-0.4 mmol/L,t = 2.33,P＜ 0.05 and+29.7μ mol/L,t =2.54,P＜0.05 vs.baseline,respectively)only in the irbesartan/HCTZ group.Conclusions Both combinations had similarly effective in reducing clinical BP in very elderly hypertensives.However,valsartan/amlodipine offered some advantage and less pronounced BP orthostatic changes and absence of metabolic adverse effects.     

罗格列酮与全反式维甲酸对人胃癌SGC7901细胞株生长和凋亡的影响

Objective To investigate the influence of PPARγ excitomotor RSG and ATRA on gastric cancer SGC7901 cell line proliferation in vitro and its potential mechanism study.Methods Human gastric cancer SGC7901 cell line was cultured in vitro.Experiment samples were divided to blank group,10μmol/L ATRA group, 12.5μmol/L RSG group, 25μmol/L RSG group, 10μmol/L ATRA + 25μmol/L RSG group.Proliferation inhibitory effect was determined by MTI assay.Flow cytometry was used to detect cell cycle, H.E stain was used to observed micrography alteration.Expression of PPARγ protein in gastric cancer cells were measured by immunohistochemistry.PPARγ mRNA in gastric cancer cells were measured by RT-PCR.Results ATAR at concentration 10μmol/L, RSG at 12.5 μmol/L and RSG at 25 μmol/L could inhibit the proliferation of SGC7901 cells in a dose-and time-dependent, and when both agents were combined for 72h, growth inhibition ratio was (29.73 ± 0.69) %.Flow cytometry analysis revealed a cell cycle arrest at G1 and S phase, and when both agents combined, S% was (12.87 ± 0.35 )%, cell micrography tended to be normal when both agents combined.Up-regulation of PPARγ protein and PPARγ mRNA expressions were also observed, those effects were enhanced when both agents combined, and grey scale ratio was 0.646.Conclusion The ATRA and RSG could significantly induced growth inhibition of human gastric cancer SGC7901 cell, which may be associated with cell cycle arrest and inducing differentiation, activation of PPARγ protein and PPARγ mRNA expression.Synergistic effect could be caused by the combined use of the two agents.     

罗格列酮与全反式维甲酸对人胃癌SGC7901细胞株生长和凋亡的影响

Objective To investigate the influence of PPARγ excitomotor RSG and ATRA on gastric cancer SGC7901 cell line proliferation in vitro and its potential mechanism study.Methods Human gastric cancer SGC7901 cell line was cultured in vitro.Experiment samples were divided to blank group,10μmol/L ATRA group, 12.5μmol/L RSG group, 25μmol/L RSG group, 10μmol/L ATRA + 25μmol/L RSG group.Proliferation inhibitory effect was determined by MTI assay.Flow cytometry was used to detect cell cycle, H.E stain was used to observed micrography alteration.Expression of PPARγ protein in gastric cancer cells were measured by immunohistochemistry.PPARγ mRNA in gastric cancer cells were measured by RT-PCR.Results ATAR at concentration 10μmol/L, RSG at 12.5 μmol/L and RSG at 25 μmol/L could inhibit the proliferation of SGC7901 cells in a dose-and time-dependent, and when both agents were combined for 72h, growth inhibition ratio was (29.73 ± 0.69) %.Flow cytometry analysis revealed a cell cycle arrest at G1 and S phase, and when both agents combined, S% was (12.87 ± 0.35 )%, cell micrography tended to be normal when both agents combined.Up-regulation of PPARγ protein and PPARγ mRNA expressions were also observed, those effects were enhanced when both agents combined, and grey scale ratio was 0.646.Conclusion The ATRA and RSG could significantly induced growth inhibition of human gastric cancer SGC7901 cell, which may be associated with cell cycle arrest and inducing differentiation, activation of PPARγ protein and PPARγ mRNA expression.Synergistic effect could be caused by the combined use of the two agents.     

硝酸甘油对大鼠重症急性胰腺炎ET/NO、TXA2/PGI2的影响

Objective To explore the effect of nitroglycerin on ET/NO, TXA2/PGI2 and pancreas pathomorphology changes in severe acute pancreatitis (SAP) rats. Methods Sixty SD rats were random divided into five groups, including control group (A group, n = 12) and experimental group(B,C,D and E group, n = 12). The SAP was induced by injection of 5% sodium taurocholate through retrograde common biliopancreatic ducts via duodenal papilla with epidural catheter. Group C, D and E were intravenously injected with nitroglycerin 0.5μg/kg/min,1μg/kg/min and 2μg/min in 30 min respectively, and group A and B was injected with Sodium Chloride 0.5ml. The indexes of changed pathomorphology and ET/NO, TXB2/6-keto-PGF1a, were determined at the 6th and 12th hour after operations, respectively. Results The specimen data of the 6th and 12th hour displayed that the indexes of changed pathomorphology, ET, ET/NO, TXB2, and TXB2/6-keto-PGF1a of the group C,D and E degraded respectively, compared to group B(P < 0.05). Conclusion The nitroglycerin could degrade ET, ET/NO,TXA2 and TXA2/PGI2, improve the microcirculation of pancreas, and delay the pathological inflammation change in SAP rats.     

利尿剂在三聚氰胺及三聚氰酸致大鼠急性肾损伤中的治疗作用

Objective To investigate the effect of diuretic (furosemide) therapy on kidney injury induced by melamine and cyanuric acid in rats. Methods 36 male Spragne Dawley rats were random disided into 3 groups. Group A was treated with 2mL of water daily, group B was treated with melamine and cyanuric acid ( each 100 mg/kg) daily for 4 days and then 2ml of water daily, group C was treated with the same as group B at the first 4 days and then treatment with furosemide (20mg/kg) daily. Samples of blood and 24h urine were collected to detective biochemical indexes, and kidney sections were performed on days 4 and 11 ( each end point, n = 6). The kidneys were observed with histopathology and renal crystal deposition scores were determined. Results On the 4th day, group B and group C were resulted in acute kidney injury such as oliguria [ ( 3. 39 ± 1.02 ) ml, ( 3. 20 ± 0. 86 ) ml ] and high serum creatinine [ ( 153.54 ±27. 08)μmol/L, (160. 11 ± 19. 55)μmol/L] and renal melamine cyanurate crystal were found in the renal tissues. On the 11th day, the renal crystal deposition score in the rats was reduced by 9. 52% ( P >0. 05). Compared with those of the 4th day in group B, it reduced by 63.63%( P <0.05) in group C. Urine volume were increased significantly compared with those of the 4th day( P < 0. 05 ) in group C [ from (3.20±0. 86)ml to (25.96 ±5.97)ml] and group B [ from(3. 39 ± 1.02)ml to (8. 57 ± 1.66)ml] , and Urine volume in group C was increased significantly more than that in group B ( P < 0. 05 ). The serum creatinine was obviously reduced as compared with those of the 4th day in group B and C( P <0.05), from[ (153. 54±27.08) μmol/L] to [ ( 106. 10 ±5.53) μmol/L] in group B and from [ ( 160. 11 ± 19. 55) μmol/L] to [ (67. 17 ± 12. 80 ) μmol/L] in group C, but the serum creatinine in group B was still higher than that in group A and C ( P < 0. 05). Conclusions Furosemide can attenuate the damage of acute kidney injury induced by melamine and cyanuric acid.     

近视眼视网膜神经纤维层厚度与中央角膜厚度相关性研究

Objective To explore the relationship between retinal nerve fiber layer (RNFL) thickness and central corneal thickness (CCT) in myopia eyes.Methods 91 cases (91eyes) were selected from ophthalmological outpatients including 28cases with low myopia ( spherical equivalence [ SE ] ＞ -3.0D), 33 cases with moderate myopia (SE -0.3D ～ -6.0D) and 30 cases with high myopia (SE ＜ -6.0D).All patients received ocular standard examination including intraocular pressure, refraction, slitlamp biomicroscopy and fundus examination.Other ocular diseases except refractive error were excluded.RNFL thickness and CCT were measured by RTVue Fourior-OCT ( Optovue Inc, USA).Refraction diopter was shown as SE.Results The mean RNFL thickness and CCT was ( 108.5 ± 10.1 ) μm, (524.7 ±36.8)μm respectively.These were no significantly different among low, moderate and high myopia ( P ＞ 0.05 ).Temporal RNFL thickness( tl1 ,tu1 ) was significantly positive related with CCT( r =0.281,0.093 of tl1, r= 0.352,0.167 of tu1 respectively in single and multiple variable analysis; P ＜ 0.05 ), nasal ( nl2, nu2)and inferior nasal RNFL thickness( in2,in1 )was significantly positive related with SE( P ＜0.05), inferior temporal RNFL thickness( it2)was significantly negative related with SE( P ＜0.05), and RNFL thickness in other regions were not significant related with CCT and SE ( P ＞ 0.05 ) in single and multiple variable regressive analysis.Conclusion Relationship between RNFL thickness of local paradisc region and CCT in myopia eyes suggested that CCT should be correlative with some sensible structural parameters in glaucomatous neuropathy and might be important in the diagnosis and therapy of glaucoma.     

近视眼视网膜神经纤维层厚度与中央角膜厚度相关性研究

Objective To explore the relationship between retinal nerve fiber layer (RNFL) thickness and central corneal thickness (CCT) in myopia eyes.Methods 91 cases (91eyes) were selected from ophthalmological outpatients including 28cases with low myopia ( spherical equivalence [ SE ] ＞ -3.0D), 33 cases with moderate myopia (SE -0.3D ～ -6.0D) and 30 cases with high myopia (SE ＜ -6.0D).All patients received ocular standard examination including intraocular pressure, refraction, slitlamp biomicroscopy and fundus examination.Other ocular diseases except refractive error were excluded.RNFL thickness and CCT were measured by RTVue Fourior-OCT ( Optovue Inc, USA).Refraction diopter was shown as SE.Results The mean RNFL thickness and CCT was ( 108.5 ± 10.1 ) μm, (524.7 ±36.8)μm respectively.These were no significantly different among low, moderate and high myopia ( P ＞ 0.05 ).Temporal RNFL thickness( tl1 ,tu1 ) was significantly positive related with CCT( r =0.281,0.093 of tl1, r= 0.352,0.167 of tu1 respectively in single and multiple variable analysis; P ＜ 0.05 ), nasal ( nl2, nu2)and inferior nasal RNFL thickness( in2,in1 )was significantly positive related with SE( P ＜0.05), inferior temporal RNFL thickness( it2)was significantly negative related with SE( P ＜0.05), and RNFL thickness in other regions were not significant related with CCT and SE ( P ＞ 0.05 ) in single and multiple variable regressive analysis.Conclusion Relationship between RNFL thickness of local paradisc region and CCT in myopia eyes suggested that CCT should be correlative with some sensible structural parameters in glaucomatous neuropathy and might be important in the diagnosis and therapy of glaucoma.     

异丙酚对大鼠肺缺血/再灌注损伤的保护作用

Objective To study the protective effects of propofol against ischemia/reperfusion injury in rat lung. Methods Rat model of pulmonary ischemia/reperfusion injury was used in this study. Rats were randomly divided into three groups, including sham opera-tion group (group A), iachemia/reperfusion group (group B) and propofol group (group C), 15 rats in each group. The concentration of tumor necrosis factor -α and interleukin-6 in bronchoalveolar lavage fluid (BALF) were measured by enzyme linked immunosorbent assay (ELISA). Then blood gas analysis, lung wet/dry (W/D) weight ratio were detected in each group. Results Propofol could significantly improve PaO2, reduce the W/D value and the contents of TNF-α and IL-6 in BALF. Conclusion Propofol effectively suppressed the pro-duction and release of inflammatory cytokine, therefore it can protect the lung from isehemia/reperfusion injury.     

传染性单核细胞增多症患儿CD3+CD4-CD8-T细胞与T淋巴细胞亚群变化情况及临床意义

Objective To study on the changes of the DNT cells and T lymphocyte subtype in children with infectious mononucleosis (IM) and its clinical significance.Methods The flow cytometry (FCM) was used to detected the DNT cells and other T lymphocyte subtype in 48 cases of IM.Results The study showed that DNT cells( 9.39 ± 4.89 )% were greatly increased in comparison with normal controls (NC) (4.26 ± 1.68)% ( P ＜0.01 ).CD4 cells(21.45 ±9.87)% were decreased ( P ＜0.01 ) and CD8 cells increased in comparison with NC (32.43 ± 5.07) % ( P ＜ 0.01 ).Conclusion DNT cells and T lymphocyte subtype can be used to evaluate the immune function of children with infectious mononucleosis (IM) and provide guidance for adoptive immunotherapy.     

强直性脊柱炎合并骨质疏松的临床研究

Objective To investigate the incidence of osteoporosis (OP) in patients with ankylosing spondylitis and the relationship between OP and the clinical data. Methods Serum levels of bone alkaline phosphatase (BALP) and tartrate resistant acid phosphatase 5b (TRACP5b) were detected by enzyme linked immunosorbent assay ( ELISA ) in 60 cases with ankylosing spondylitis, and it was compared with normal controls. Bone mineral density (BMD) was measured through dual-energy X-ray absorptiometry ( DXA), including lumbar ( L2 - L4), bilateral femoral neck and greater trochanter. Some clinical data was collected and analyzed at the same time. Results The incidence of OP in AS patients was 35%, and the incidence of OP in the femoral proximal end was higher than that in lumbar. Compared with normal controls[ ( 1.06 ±0. 18 )U/L ], the levels of serum TRACP5b in AS[ (1.31 ± 0. 82 )U/L] patients was significantly higher ( P <0. 05 ). The levels of serum BLAP in OP combined AS group[ ( 21.65 ± 5.41 ) U/L]were significantly lower than non-OP group[ (32. 37 ± 16. 5 ) U/L] ( P <0. 05 ). The disease duration was negatively correlated with the BMD of femoral neck ( P < 0. 01 ). Conclusions There was higher incidence of OP in AS patients, which were related with the abnormality of bone metabolism and the disease duration.Multiple factors participated in the regulation of bone metabolism of AS.